全文获取类型
收费全文 | 23082篇 |
免费 | 1889篇 |
国内免费 | 865篇 |
专业分类
耳鼻咽喉 | 91篇 |
儿科学 | 232篇 |
妇产科学 | 314篇 |
基础医学 | 1881篇 |
口腔科学 | 289篇 |
临床医学 | 1391篇 |
内科学 | 2345篇 |
皮肤病学 | 213篇 |
神经病学 | 872篇 |
特种医学 | 1324篇 |
外国民族医学 | 4篇 |
外科学 | 652篇 |
综合类 | 3703篇 |
预防医学 | 1520篇 |
眼科学 | 391篇 |
药学 | 6835篇 |
13篇 | |
中国医学 | 2278篇 |
肿瘤学 | 1488篇 |
出版年
2024年 | 61篇 |
2023年 | 334篇 |
2022年 | 838篇 |
2021年 | 998篇 |
2020年 | 766篇 |
2019年 | 646篇 |
2018年 | 598篇 |
2017年 | 695篇 |
2016年 | 752篇 |
2015年 | 828篇 |
2014年 | 1289篇 |
2013年 | 1384篇 |
2012年 | 1457篇 |
2011年 | 1487篇 |
2010年 | 1272篇 |
2009年 | 972篇 |
2008年 | 1125篇 |
2007年 | 1213篇 |
2006年 | 1037篇 |
2005年 | 948篇 |
2004年 | 823篇 |
2003年 | 740篇 |
2002年 | 615篇 |
2001年 | 544篇 |
2000年 | 504篇 |
1999年 | 374篇 |
1998年 | 314篇 |
1997年 | 308篇 |
1996年 | 243篇 |
1995年 | 223篇 |
1994年 | 234篇 |
1993年 | 166篇 |
1992年 | 197篇 |
1991年 | 247篇 |
1990年 | 224篇 |
1989年 | 148篇 |
1988年 | 163篇 |
1987年 | 129篇 |
1986年 | 112篇 |
1985年 | 170篇 |
1984年 | 168篇 |
1983年 | 141篇 |
1982年 | 96篇 |
1981年 | 68篇 |
1980年 | 59篇 |
1979年 | 47篇 |
1978年 | 30篇 |
1977年 | 16篇 |
1976年 | 10篇 |
1972年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
《Drug discovery today》2022,27(6):1733-1742
Compounds that exhibit assay interference or undesirable mechanisms of bioactivity are routinely encountered in assays at various stages of drug discovery. We observed that assays for the investigation of thiol-reactive and redox-active compounds have not been collected in a comprehensive review. Here, we review these assays and subject them to experimental optimization to improve their reliability. We demonstrate the usefulness of our assay cascade by assaying a library of bioactive compounds, chemical probes, and a set of approved drugs. These high-throughput assays should complement the array of wet-lab and in silico assays during the initial stages of hit discovery campaigns to pursue only hit compounds with tractable mechanisms of action. 相似文献
3.
薄层色谱鉴别在历版《中国药典》中的应用经历了从无到有、从少到多的过程;而薄层扫描含量测定在最近几版《中国药典》中的应用比例逐渐降低。随着对中药质量标准体系要求的进一步提高,薄层色谱法的不足之处陆续显现,如仪器普及率低、设备并不简单、结果重复性和稳定性较差、鉴别速度及准确性不及高效液相色谱法、展开剂毒性大等,逐渐不合时宜。在制定中药质量标准时,研究者不应该墨守成规,薄层色谱鉴别也不应该是雷打不动的定性鉴别必备选项。高效液相色谱法具备完全取代薄层色谱法的可行性,薄层色谱法可作为高效液相色谱法的补充。为充分降低检测成本、缩短检测周期、提高鉴别效率,笔者建议中药质量标准体系应该大幅减少薄层色谱鉴别方法的应用,增加高效液相特征图谱鉴定,尽量做到“一个条件,一张图谱”;除非确有必要,《中国药典》等国家质量标准体系应将薄层色谱鉴别作为推荐方法,而非强制标准。 相似文献
4.
5.
《Drug metabolism and pharmacokinetics》2019,34(1):87-94
The purpose of this study was to elucidate the involvement of Mate1 in the tubular secretion of trimethoprim and saturation of Mate1-mediated efflux to address the mechanisms underlying the pharmacokinetic drug interactions with trimethoprim. Trimethoprim is a more potent inhibitor of MATE2-K than MATE1 with Ki values (μM) of 0.030–0.28 and 2.4–5.9, respectively. Trimethoprim is a substrate of human MATE1 and MATE2-K with Km values of 2.3 ± 0.9 and 0.018 ± 0.004 μM, and mouse Mate1, but not human OCT2, mouse Oct1 and Oct2. Pyrimethamine significantly reduced the renal clearance (CLR) of trimethoprim (mL/min/kg) from 40.0 ± 5.1 to 20.1 ± 3.7 (p < 0.05). Trimethoprim was given to mice at three infusion rates (150, 500, and 1500 nmol/min/kg). Together with an increase in the plasma concentrations of trimethoprim, the CLR (mL/min/kg) of trimethoprim decreased to 25.9 ± 3.2, 13.5 ± 5.7, and 8.92 ± 1.50 at the respective rates. Trimethoprim decreased the CLR of rhodamine 123 in an infusion rate-dependent manner: 11.5 ± 1.3 (control), 5.17 ± 1.55, 1.31 ± 0.50, and 0.532 ± 0.180. These results suggest that Mate1 mediates the tubular secretion of trimethoprim, and at therapeutic doses, MATEs-mediated efflux can be saturated, and thereby, cause drug interactions with other MATE substrates. 相似文献
6.
目的研究并比较不同规格怀牛膝不同极性部位的HPLC指纹图谱,探索其内部质量差异,为该药材的规格标准完善及临床用药提供参考。方法将怀牛膝用体积分数75%乙醇水浴回流提取,得体积分数75%乙醇回流提取物,用40 mL水溶解后,依次用石油醚、三氯甲烷、乙酸乙酯、正丁醇进行萃取,得到各萃取相及萃取后的水相,浓缩至浸膏,采集各部位HPLC指纹图谱;运用相似度、综合聚类法进行数据分析,同时对其不同部位的指纹图谱进行比对分析。结果石油醚、氯仿部位均标定了11个共有峰,乙酸乙酯部位标定了10个共有峰,正丁醇部位标定了19个共有峰,水部位标定8个共有峰;氯仿部位指纹图谱相似度差异较大,其他部位指纹图谱相似度差异较小,均在0.9以上;石油醚部位指纹图谱差异主要体现在峰高,乙酸乙酯部位、氯仿部位、水部位的化学成分种类及峰高均存在差异;综合聚类分析能将不同规格怀牛膝区分开。结论不同规格怀牛膝内部质量存在差异;实验中所建立的HPLC指纹图谱可以全面反映不同规格怀牛膝的化学成分分布,为不同规格怀牛膝的整体质量评价提供参考。 相似文献
7.
8.
As a consequence of the time-varying magnetic field induced by eddy currents, frequency drifting occurs when the sampling window of localized spectroscopy continuously shifts. The frequency drifting and the concomitant phase variations can severely affect spectroscopy results when data are acquired with multiple echo times (TEs), such as in the measurement of glutamate (Glu) concentration using the TE-averaged method. Specifically, the averaged spectra are further broadened and distorted in the presence of residual eddy currents, and editing of the coupled spins of Glu C4 protons is affected, resulting in errors in the measured relative intensity ratio. Postacquisition correction using unsuppressed water as reference can effectively minimize this detrimental effect, as manifested by the significantly enhanced signal intensity. Also, it is demonstrated that the methyl signals of creatine (Cr) at 3.0 ppm and choline (Cho) at 3.2 ppm can be used as internal references in finding frequency and phase disparities between different TEs. 相似文献
9.
By substituting the saturated vapour phase tension of the pure normal hydrocarbons described by the Clausius-Clapeyron law into the accepted expression of the specific retention volume (Vg,T), a theoretically coherent and relatively simple mathematical evidence of the elution behaviour of the homologous members has been deduced. It gives exponential retention time dependence on carbon number for isothermal, and nearly equidistant (i.e., approximately linear retention-time dependence on carbon number) elution for linear temperature programmed gas chromatographic runs. The final equations are in close correlation with the experimental results. Special emphasis is placed on the fact that a good approximation—not strict physical laws—have been found. 相似文献
10.