糖性白内障大鼠晶状体水不溶性蛋白的改变

用D一半乳糖诱发了大鼠白内障,分析了其膜固有蛋白和尿素溶蛋白的改变。对正常大鼠晶状体纤维细胞膜蛋白电泳图谱的分析表明,其26KDa的主要膜固有蛋白(MIP26)约占膜固有蛋白总量的42.9％,22KDa的膜固有蛋白约占6.4％。糖性白内障大鼠晶状体纤维细胞膜固有蛋白以22KDa为主,约占30.5％,MIP26几乎消失。并在24KDa处有一深染区带。在白内障发病过程中18KDa的膜蛋白的相对含量无明显改变。这些结果提示在糖性白内障发病过程中MIP26发生了降解,产生了22KDa的降介产物。 以每克湿重计,白内障晶状体尿素溶蛋白降低约22.5％。但白内障晶状体尿素溶蛋白占总蛋白的比例升高。正常大鼠尿素溶蛋白电泳图谱主要显示四条区带,其近似分子量分别为22.5KDa、22.4KDa、20.6KDa和19.2KDa。白内障晶状体20.6KDa和19.2KDa的二条区带所占的比例明显减小。这个结果提示正常晶状体尿素溶蛋白主要含有α—和β—晶体蛋白,而且白内障晶状体尿素溶蛋白则主要含有β—晶体蛋白。     

α_1—抗胰蛋白酶异质体的测量新方法及其临床意义的探讨

本文对a_1-抗胰蛋白酶异质体的检测方法(小扁豆凝集素亲合交叉免疫电泳法)进行改进。改进后的方法操作流程短、成本低、结果清晰、操作简便、不需预测a_1-抗胰蛋白酶总浓度,适合于临床广泛应用。本文用该法对原发性肝癌、转移性肝癌,其它器官癌肿、肝硬化及正常人血清中的a_1-抗胰蛋白酶异质体进行分析,发现小扁豆凝集素结合型a_1-抗胰蛋白酶百分比(LCA-R-AAT％)原发性肝癌组明显高于其它各组,以LCA-R-AAT(％)>25％为阳性标准,则原发性肝癌诊断的敏感性、特异性、准确性分别为80.6％、86.2％和84.3％,与小扁豆凝集素结合型甲胎蛋白联合检测,可使原发性肝癌诊断的敏感性提高至100％(P<0.05),特异性和准确性分别为82.8％和89.9％(P均>0.05),表明LCA-R-AAT(％)的检测对原发性肝癌的诊断及鉴别诊断有一定的价值。     

有晶状体眼虹膜夹前房型人工晶状体植入矫治高度近视

有晶状体眼虹膜夹前房型人工晶状体植入矫治高度近视是矫治高度近视的一种新方法,因有良好的术后视觉质量、保留调节力、潜在的可逆性等优点,在国内已进入临床应用阶段。本文就此种晶状体的发展历史、该技术安全性、有效性、可预测性、准确性、稳定性,及其展望作一综述。     

miR-211对高糖诱导下晶状体上皮细胞氧化应激反应的调控作用

目的 探讨miR-211对高糖诱导下晶状体上皮细胞氧化应激反应的调控作用及其可能存在的作用机制.方法 将HLEC-B3细胞在含不同浓度(0、10、20、40 mmol/L)葡萄糖的培养基中培养48 h,RT-PCR检测细胞miR-211表达,Western blot检测细胞SIRT1蛋白表达,试剂盒检测总抗氧化能力(T...     

Generation of a Soluble African Horse Sickness Virus VP7 Protein Capable of Forming Core-like Particles

A unique characteristic of the African horse sickness virus (AHSV) major core protein VP7 is that it is highly insoluble, and spontaneously forms crystalline particles in AHSV-infected cells and when expressed in vitro. The aggregation of AHSV VP7 into these crystals presents many problems in AHSV vaccine development, and it is unclear whether VP7 aggregation affects AHSV assembly or contributes to AHSV pathogenesis. Here, we set out to abolish VP7 self-assembly by targeting candidate amino acid regions on the surface of the VP7 trimer via site-directed mutagenesis. It was found that the substitution of seven amino acids resulted in the complete disruption of AHSV VP7 self-assembly, which abolished the formation of VP7 crystalline particles and converted VP7 to a fully soluble protein still capable of interacting with VP3 to form core-like particles. This work provides further insight into the formation of AHSV VP7 crystalline particles and the successful development of AHSV vaccines. It also paves the way for future research by drawing comparisons with similar viral phenomena observed in human virology.     

糖尿病患者白内障摘除人工晶体植入的疗效观察

目的 探索糖尿病患者的白内障摘除及人工晶体植入术的疗效。方法 对６８例（８２眼）糖尿病患者白内障和６８例（７８眼）老年性白内障行晶体囊外摘除后房型人工晶体植入术,从术后视力、术后并发症进行比较。结果 术前没有或仅有轻微视网膜病变的糖尿病患者术后视力恢复与老年性白内障患者无明显差别（Ｐ〉０．０５）;糖尿病患者术后眼前节炎症性反应明显增高（Ｐ〈０．０１）;术后发生糖尿病性视网膜病变进展者皆为血糖控制不     

92只眼非球面人工晶状体囊袋内植入后偏心与倾斜临床分析

[目的]比较2种非球面人工晶状体（IOL）在囊袋内植入后偏心与倾斜的差异,探讨IOL的攀设计与特性对在囊袋内稳定性的影响．[方法]选择行超声乳化白内障摘除联合人工晶状体植入术的老年性白内障患者61例（92只眼）,随机分为2组植入L形Acrysof IQ SN60WFIOL（50眼）和一体四点Akreos Adapt AO IOL（42眼）．术后6个月用眼前节成像系统Pentacam采集各眼的Scheimpflug图像,分析得出IOL的偏心值与倾斜度．[结果]AcrysofIQSN60WFIOL和AkreosAdaptAOIOL两组患者IOL的水平偏心值分别为（0．21±0．05）mm,（0．17±0．04）mm,相比较差异有统计学意义（P=0．021）．两组患者IOL的垂直与水平方向倾斜度分别为1．72°±0．76°,1．51°±0．57°,1．64°±0．71°,1.59°±0．41°,差异均无统计学意义（P=0．674,P=0．521）．两组患者的最佳矫正视力和对比敏感度差异亦无统计学意义（P〉0．05）．[结论]2种IOL囊袋内植入后在倾斜度方面无明显差异,而在居中性方面,AcrysofIQSN60WFIOL明显优于AkreosAdaptAOIOL,不同非球面IOL的襻设计可影响在囊袋内的稳定性．     

Outbreak of silicosis in Spanish quartz conglomerate workers

Abstract

Objectives: To describe the epidemiological and clinical characteristics of an outbreak of occupational silicosis and the associated working conditions.

Methods: Cases were defined as men working in the stone cutting, shaping, and finishing industry in the province of Cádiz, diagnosed with silicosis between July 2009 and May 2012, and were identified and diagnosed by the department of pulmonology of the University Hospital of Puerto Real (Cádiz). A census of workplaces using quartz conglomerates was carried out to determine total numbers of potentially exposed workers. A patient telephone survey on occupational exposures and a review of medical records for all participants were conducted.

Results: Silicosis was diagnosed in 46 men with a median age of 33 years and a median of 11 years working in the manufacturing of countertops. Of these cases, 91·3% were diagnosed with simple chronic silicosis, with an abnormal high-resolution computerized tomography (HRCT) scan. One patient died during the study period. Employer non-compliance in prevention and control measures was frequently reported, as were environmental and individual protection failures.

Conclusions: The use of new construction materials such as quartz conglomerates has increased silicosis incidence due to intensive occupational exposures, in the context of high demand fuelled by the housing boom. This widespread exposure poses a risk if appropriate preventive measures are not undertaken.     

Corneal injection track:an unusual complication of intraocular lens implantation and review

Phacoemulsification is the main gold standard for cataract operation in the developed world together with foldable intraocular lens (IOL) implantation by injection, allowing for stable wound construction and less postoperative astigmatism. It is a safe procedure with high success rate with the advancement in machines, improvement of IOL injection systems and further maturation of surgeons’ techniques. Despite the large number of operations performed every day, foldable IOL injection leading to an intra-stromal corneal track is a very rare complication. We report a case of this unusual finding in a 70-year-old gentleman who has undergone cataract operation in November 2011 in our hospital and will review on the complications related to foldable IOL injection.