Fabrication of tissue‐engineered cell sheets by automated cell culture equipment

Most cells for regenerative medicine are currently cultured manually. In order to promote the widespread use of regenerative medicine, it will be necessary to develop automated culture techniques so that cells can be produced in greater quantities at lower cost and with more stable quality. In the field of regenerative medicine technology, cell sheet therapy is an effective tissue engineering technique whereby cells can be grafted by attaching them to a target site. We have developed automated cell culture equipment to promote the use of this cell sheet regenerative treatment. This equipment features a fully closed culture vessel and circuit system that avoids contamination with bacteria and the like from the external environment, and it was designed to allow 10 cell sheets to be simultaneously cultured in parallel. We used this equipment to fabricate 50 sheets of human oral mucosal epithelial cells in five automated culture tests in this trial. By analyzing these sheets, we confirmed that 49 of the 50 sheets satisfied the quality standards of clinical research. To compare the characteristics of automatically fabricated cell sheets with those of manually fabricated cell sheets, we performed histological analyses using immunostaining and transmission electron microscopy. The results confirmed that cell sheets fabricated with the automated cell culture are differentiated in the same way as cultures fabricated manually.     

医用紧凑型回旋加速器水冷系统设计

本文介绍了一种医用紧凑型回旋加速器水冷系统的设计和实现方法。该系统采用双循环结构以确保制冷量和温控精度,通过混床旁路去离子处理满足电导率要求,在确保设计参数的前提下,还考虑到整体结构的紧凑性。此外,开发了基于LabVIEW的人机界面,以实现系统的便捷监控,有利于与其他系统的集成。应用结果表明,该水冷系统能够满足医用加速器各子系统冷却需求,保证了设备的正常运行。     

骨科患者病历管理数据库系统的设计

为了提高医院骨科临床工作人员的业务水平,向科研人员提供大量的实际病例资料,为教师和学生提供丰富的题材,本文针对本地区骨科患者的具体情况,结合国际骨科疾病的分类与分型标准,提出了一种用数据库实现骨科患者病历管理的方法。首先给出了骨科患者病历管理数据库系统程序架构设计,将系统分为骨创、脊柱外科、关节外科等多个模块,并对每一个模块又进一步详细分型;然后对程序主界面和程序功能进行设计;最后对系统进行测试。测试结果显示,该骨科患者病历管理数据库系统的设计可以满足临床,科研和教学的要求。     

慢性病患者生命质量测定量表共性模块的难度与区分度再次评价

目的用经典测量理论(Classical test theory,CTT)分析慢性病患者生命质量测定量表体系共性模块(QLICD-GM)每个条目的难度与区分度。方法调查8种慢性病的1024例患者,采用经典测量理论计算每个条目的难度、区分度系数,采用SPSS软件进行计算和作图。结果共性模块条目难度适中,不是太难也不是太简单。条目的区分度总体适中,但是个别条目区分度过低,比如生理领域的条目PH2、PH6、PH7,需要对条目加以考虑。结论慢性病患者生命质量测定量表体系共性模块具有较好的难度和区分度,可以作为我国慢性病患者生命质量的测评工具,但QLICD-GM(V1.0)的理论结构还有待进一步补充完善。     

野战方舱医院抗震救灾妇产专业模块化抽组体会

目的:探讨野战方舱医院妇产模块化人员及装备的配置。方法:结合某野战方舱医院参加四川汶川及青海玉树抗震救灾,在灾区设立妇产模块,成立妇产科门诊及妇产科检查室。结果:在汶川和玉树抗震救灾中,通过设立妇产科模块,极大地提高了灾区妇产科伤病员的诊治水平。结论:野战方舱医院设立妇产科模块,是适应野战方舱医院执行抗震救灾的需要。     

Combined Radiofrequency Ablation-Cooling Catheter for Reversible Cryothermal Mapping and Ablation

Reversible cryothermal mapping of cardiac arrhythmias has beenperformed intraoperatively. However, a steerable cooling catheter forreversible mapping has not yet been developed. We therefore developed andtested a cooling system consisting of a +15°C hypertonic salinereservoir and a 7F steerable catheter also capable of radiofrequency (RF)ablation. Using excised ovine hearts placed in a 37°C circulating salinebath, we measured the temperatures at depths of 0 mm, 1 mm, and 2 mm. Thetemperature after 90 seconds of cooling was 16.5 ± 2.1°C at 0 mmcompared to 23.9 ± 4.1°C at 1 mm and 31.1 ± 3.9°C at 2mm depth (p < 0.01). These data suggest that a 7F steerable combined RFablation–cooling catheter may achieve temperatures suitable formapping arrhythmias such as atrial tachycardias and right ventricularoutflow tract tachycardias. Further enhancements to achieve lowertemperatures at depth may be needed to reversibly map other arrhythmiassuch as left ventricular tachycardias.     

基于医院信息系统的死亡病例报告模块应用研究

北京市卫生局京卫办字[2013]78号文件,《北京市卫生局关于开展死亡病例报告管理模块研发试点工作的通知》中,拟定北京协和医院等五家医院开展HIS系统死亡病例报告管理模块研发的试点工作。信息化死亡病例报告系统本着简化流程、提高效率、保证安全的原则进行设计。模块的功能包括证书编号和基本信息的自动获取、逻辑校验、电子签名、统计功能等方面。电子化死亡病例报告模块的推广和使用,还必须结合各医疗机构自身工作特点,同时与国家疾控中心、民政部、公检法等相关部门的多方沟通,获得认可后方可大规模推广和使用。     

郑小伟教授辨治支气管扩张症经验

支气管扩张症的病机为本虚标实,整体的亏虚与局部的邪实并存,可因实致虚,也可因虚生实。治疗上当辨清邪正虚实,标本缓急,分期论治。急性期当泻火凉血治其标,化痰行瘀清其源;缓解期宜滋阴润肺治其本。     

Wire Arc Additive Manufacturing of Al-Mg Alloy with the Addition of Scandium and Zirconium

The proposed paper considers the opportunity of expanding the application area of wire arc additive manufacturing (WAAM) method by means of increasing the strength properties of deposited material, due to the implementation of aluminum wire with the addition of scandium and zirconium. For the experimental research, the welding wire 1575 of the Al-Mg-Sc-Zr system containing 0.23% Sc and 0.19% Zr was selected. The optimal welding parameters, ensuring the defect-free formation of deposited material with low heat input, were used. Porosity level was estimated. The thermal state was estimated by finite element simulation. Simulated thermal state was verified by comparison with thermocouples data. Post-heat treatment parameters that lead to maximum strength with good plasticity were determined. The maximum yield strength (YS) of 268 MPa and ultimate strength (UTS) of 403 MPa were obtained, while the plasticity was determined at least 16.0% in all WAAM specimens.     

Coupling Raman,Brillouin and Nd3+ Photo Luminescence Spectroscopy to Distinguish the Effect of Uniaxial Stress from Cooling Rate on Soda–Lime Silicate Glass

Evolution of spectroscopic properties of a soda–lime silicate glass with different thermal history and under applied uniaxial stress was investigated using Raman and Brillouin spectroscopies as well as Nd³⁺ photoluminescence techniques. Samples of soda–lime silicate with a cooling rate from 6 × 10⁻⁴ to 650 K/min were prepared either by controlled cooling from the melt using a differential scanning calorimeter or by a conventional annealing procedure. Uniaxial stress effects in a range from 0 to −1.3 GPa were investigated in situ by compression of the glass cylinders. The spectroscopic observations of rearrangements in the network structure were related to the set cooling rates or the applied uniaxial stress to calculate an interrelated set of calibrations. Comparing the results from Raman and Brillouin spectroscopy with Nd³⁺ photoluminescence analysis, we find a linear dependence that can be used to identify uniaxial stress and cooling rate in any given combination concurrently. The interrelated calibrations and linear dependence models are established and evaluated, and equations relating the change of glass network due to effects of cooling rate or uniaxial stress are given.