补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究

目的:运用中药血清药理学方法研究补肾复方含药血清(BS)促进体外培养的PC12细胞增殖及其拮抗谷氨酸兴奋性神经毒损伤的作用.方法:以MTT法观察BS对体外培养的PC12细胞活力的影响及其拮抗谷氨酸损伤的作用,采用流式细胞术观察BS对谷氨酸所诱导PC12细胞凋亡的影响.结果:BS含药血清可以增强PC12细胞活力,拮抗谷氨酸的兴奋性神经毒作用且在20%浓度时作用最强,并且能够抑制谷氨酸诱导的PC12细胞早期凋亡.结论:补肾方BS可能具有神经保护作用.     

乐胃饮干预功能性消化不良大鼠SP和VIP的研究

[目的]通过实验研究,揭示乐胃饮治疗功能性消化不良的作用机制。[方法]Wistar大鼠随机分为正常对照组、自然恢复组、吗叮啉组、参苓白术丸组、乐胃饮低剂量组、乐胃饮中剂量组、乐胃饮高剂量组等7组,每组6只。分别给予各组按2ml/100g按组分别灌服蒸馏水、吗叮啉、参苓白术丸液、乐胃饮低中高剂量液,每天1次,自造模结束后一天起连续给药14天。观察胃黏膜形态,检测胃肠激素SP和VIP。[结果]乐胃饮能有效增加实验鼠胃窦组织中SP和降低VIP含量。[结论]乐胃饮能提高胃肠道神经兴奋性,调节胃肠道平滑肌的收缩,这可能是乐胃饮治疗FD的主要作用机制。     

当归六黄汤加减对32例混合痔术后盗汗患者临床疗效观察

[目的]探讨当归六黄汤加减对混合痔术后盗汗的疗效。[方法]对32例混合痔术后盗汗患者采用当归六黄汤加减。[结果]治愈11例(占34·4%),显效16例(占50·0%),有效5例(占16·6%)。[结论]当归六黄汤能有效治疗混合痔术后盗汗。     

Stretch of mammalian nerve in vitro: effect on compound action potentials

Stretch of nerve has been reported to decrease the amplitude of the compound action potential (CAP) with a complete block appearing in approximately 30 minutes. But for the most part, those experiments were carried out in vivo, and it is generally accepted that the failure of responses was due to a closure of vessels supplying the nerve with a resulting ischemia and anoxia. These studies were undertaken to determine if stretch of nerve has effects that are independent of interference with its vascular supply. In the studies, lengths of rat sciatic and dog peroneal nerves were removed and placed in a chamber supplied with oxygen in which their CAPs were continuously elicited and recorded. This in vitro preparation obviated interference with the nerve's metabolism on stretching. We have previously shown that the form change termed 'beading,' appearing within 10 seconds and reversing as quickly on relaxation, can be elicited with tensions of only several grams. We wished to determine if stretch adequate to produce beading could alter CAPs with the same rapidity. Tensions below 2 g had little effect. On applying tensions of 10-100 g, levels well above those needed to bead the fibers, both increases and decreases of CAP amplitude were seen. The changes occurred within 10 seconds of stretch application, the time at which beading arises with stretch. Although the decreases of CAP amplitudes could be accounted for by beading, the degree of CAP change did not correspond to the amount of tension applied. We hypothesize that the constrictions in the beaded fibers increase axial resistivity and diminish local currents so as to block conduction. The lack of an increasing degree of decreased CAP amplitude with increases in tension is ascribed to the inhibition of elongation offered by the collagen fibrils present in nerve. Collagenase applied to nerves allowed a further increase in length, producing a 'hyperbeading,' showing much longer lengths of beading constrictions on stretch. This would further increase axial resistance and is taken to account for the greater decreases of CAP amplitudes seen following collagenase treatment. To account for those cases where increases of CAP amplitude were seen on stretch, we hypothesize that stretch can also cause an increase in the excitability of the nodes. The outcome of stretch in any given nerve would be the resultant of two opposing actions; beading of the internodes causes a decrease of local currents leading to block of CAPs, while an increased excitability of the nodes acts to augment the responses.     

复方苍术汤治疗老年肥胖或超重合并糖耐量异常32例临床观察

目的:观察复方苍术汤治疗老年肥胖或超重合并糖耐量异常(IGT)的疗效.方法:以复方苍术汤治疗老年肥胖或超重合并IGT患者32例,并与盐酸二甲双胍治疗30例作对照,对治疗前后患者体重、腰围、臀围、腰臀比(WHR)、糖耐量、空腹血清胰岛素水平、血脂的变化进行对比观察.结果:复方苍术汤组治疗后患者的体重、腰围、臀围及WHR、糖耐量试验的血糖水平、空腹血清胰岛素水平、血脂与治疗前比较明显降低(P＜0.01或P＜0.05),与对照组比较差异无显著性(P＞0.05).结论:复方苍术汤对肥胖或超重合并IGT患者的体重、腰围、臀围及WHR、血糖、空腹血清胰岛素水平、血脂有明显降低作用,可用于老年肥胖或超重合并IGT患者的减重及干预治疗.     

葛根通络饮治疗糖尿病脑梗死36例临床观察

目的:观察中药葛根通络饮治疗糖尿病性脑梗死的临床疗效.方法:将72例糖尿病脑梗死患者随机分为治疗组36例,对照组36例,对照组采用常规治疗,治疗组在常规治疗基础上加服葛根通络饮,于治疗前后观察临床疗效,并检测血糖、血脂和血液流变学参数.结果:治疗组总有效率94.44%,对照组为72.22%,差异有显著性(P＜0.05);治疗组显效率63.89%,明显高于对照组(36.11%,P＜0.05).治疗组神经功能缺损有明显改善(P＜0.01),血糖、血脂和血液流变学参数均有明显改善(P<0.05或P<0.01).结论:葛根通络饮与常规治疗合用对糖尿病脑梗死有显著疗效.     

养阴清热煎剂对系统性红斑狼疮患者黏附分子表达及狼疮损伤指数的影响

目的:研究中医药对系统性红斑狼疮(SLE)的治疗作用机制及其对SLE器官损害的远期疗效.方法:1999～2003年住院及门诊的SLE患者51例,随机分为以中药养阴清热煎剂为主的中西医结合治疗组(简称治疗组)和单纯西药治疗的对照组(简称对照组),进行了治疗前后血清细胞黏附分子1(ICAM-1)、黏附分子3(ICAM-3)、P-选择素以及SLE损伤指数(SLICC/ACRDI)的研究.结果:SLE患者的ICAM-1、ICAM-3、P-选择素均较正常人明显增高;治疗组治疗后ICAM-1、P-选择素比对照组明显下降;而SLE损伤指数积分显示,治疗组比较稳定,对照组较治疗前有明显增加.结论:提示以中药养阴清热煎剂为主的治疗组对SLE患者的血清黏附分子高表达有明显的抑制作用,并对SLE的器官损害有明显的保护与修复作用.     

中药复方研究中指纹图谱的应用

本文综述了指纹图谱在中药复方研究中的作用以及中药复方研究的新思路，为中药复方的开发研究以及指纹图谱用于质量控制方面的研究提供借鉴。     

复方三维亚油酸胶丸Ⅰ中甲基橙皮苷和维生素C的HPLC分析

目的:建立测定复方三维亚油酸胶丸Ⅰ中甲基橙皮苷和维生素 C 的反相高效液相色谱法,并用该方法测定2种不同厂家的药品。方法:采用反相高效液相色谱法,以 Zorbax SB-C_(18)(150 mm×4.6mm,3.5μm)为色谱柱;甲醇-0.03mol·L~(-1)磷酸二氢钠水溶液(pH=2.5)(53:47)为流动相;流速为1.0 mL·min~(-1);检测波长为220 nm 和242 nm;柱温为30℃。结果:甲基橙皮苷在0.64～3.84μg 范围内线性关系良好,其高、中、低3个量的平均加样回收率分别为99.40%,98.18%,97.82%;RSD 分别为0.6%,0.9%,1.0%。维生素 C 在0.50～3.00μg 范围内线性关系良好,其高、中、低3个量的平均加样回收率分别为98.67%,99.22%,96.00%;RSD 分别为0.3%,0.7%,0.4%。结论:该法是一种快速、简便、准确的分析方法,可以为复方三维亚油酸胶丸Ⅰ的质量控制提供科学依据。     

健脾降脂汤治疗高脂血症28例临床观察

目的 :观察中药健脾降脂汤治疗高脂血症的疗效。方法 :将56例患者随机分两组。治疗组28例 ,采用中药健脾降脂汤内服 ;对照组28例服舒降之(辛伐他汀)治疗。治疗2个月后 ,观察两组对甘油三酯(TG)、血清总胆固醇(TC)、高密度脂蛋白(HDL_C)的改善情况 ,以及药物的不良反应率。结果 :治疗组对甘油三酯、高密度脂蛋白的改善明显优于对照组(P<0.05) ,治疗组的不良反应率也明显低于对照组 ,但两组对胆固醇的改善程度相当。结论 :健脾降脂汤治疗高脂血症的疗效显著 ,不良反应率低。