补肾鹿仙汤合胞二磷胆碱治疗神经系统损伤疗效观察

目的观察补肾鹿仙汤合胞二磷胆碱治疗神经系统损伤的疗效。方法将55例神经系统损伤患者按就诊顺序分为2组,治疗组予补肾鹿仙汤合胞二磷胆碱治疗,对照组单用胞二磷胆碱治疗,疗程均为90 d。观察2组治疗前后意识、感觉、运动等多方面情况及脑电地形图、肌电图变化。结果治疗组愈显率61%,总有效率89%;对照组愈显率35%,总有效率75%,2组比较有显著性差异。脑电地形图及肌电图治疗后2组比较均有显著性差异(P均0.05)。结论补肾鹿仙汤合胞二磷胆碱治疗神经系统损伤有较好的临床疗效。     

口服胞磷胆碱钠片联合中药治疗弱视的疗效

目的：探讨胞磷胆碱联合中药治疗弱视的疗效。方法66例弱视患者,随机分为2组,对照组33例给予常规物理疗法加中药,治疗组33例在常规物理疗法加中药基础上联合胞磷胆碱钠0．2g,3次/d,对视力等结果进行统计分析。结果治疗组对不同年龄弱视均有效,且视力,视敏度指标与对照组差异均有统计学意义( P<0．05)。结论胞磷胆碱钠联合中药治疗弱视效果较好。     

胞二磷胆碱、氯脂醒、神经节苷脂对大鼠癫(癎)的影响

目的 探讨胞二磷胆碱、氯脂醒、神经节苷脂(GM1)对青霉素致(癎)大鼠行为学及脑皮层电图的影响,为临床治疗颅脑损伤和外伤后癫(癎)提供理论依据.方法 制作大鼠癫(癎)模型,观察并比较对照组、模型组、胞二磷胆碱组、氯脂醒组、GM1组大鼠的行为学及脑皮层电图表现.结果 胞二磷胆碱、氯脂醒、GM1均不能阻止青霉素诱导的大鼠癫(癎)发作.胞二磷胆碱、GM1可以延长青霉素诱导的大鼠癫(癎)(癎)波潜伏期、阵挛强直波潜伏期,减少阵挛强直总时间.结论 胞二磷胆碱、氯脂醒、GM1均不能阻止青霉素诱导的大鼠癫(癎)发作;胞二磷胆碱、GM1可以延长青霉素诱导的大鼠癫(癎)(癎)波潜伏期、阵挛强直波潜伏期,减少青霉素腹腔注射后50 min内的阵挛强直总时间.     

高效液相色谱法测定胞磷胆碱钠注射液的有关物质

目的:建立胞磷胆碱钠注射液中有关物质的HPLC测定方法。方法:HPLC法：色谱柱为phenomener ODS2，流动相：0．05mol／L磷酸盐缓冲液，检测波长为280nm，流速为1．0ml／min。结果:样品中最相邻两杂质峰R＝1．8，胞苷酸回收率99．2％，胞磷胆碱钠线性范围为2．06--14．4μg，最低检测浓度为0．06μg/ml，胞苷酸线性范围为0．02—0．18μg，最低检测浓度为0．002μg/ml。结论：该方法简单、准确、灵敏度高。     

穴位注射胞二磷胆碱对创伤性脑损伤大鼠神经功能障碍的影响

目的：观察足三里穴位注射胞二磷胆碱疗法对创伤性脑损伤大鼠神经功能的影响。方法：成年健康雄性SD大鼠40只,采用改进的Feeney法建立大鼠脑损伤模型,设假手术组大鼠8只（A组）,造模成功大鼠32只随机分为穴位注药组（B组）、穴位注水组（c组）、腹腔给药组（D组）和对照组（E组）各8只。造模后每日B、C、D、E组分别给予足三里穴位注射胞二磷胆碱及生理盐水、腹腔注射胞二磷胆碱及生理盐水处理,连续14d。采用神经功能缺损评分、斜板试验及平衡试验观察造模后第1、2、4、6、8、10、12、14天各处理方法对大鼠的神经行为的影响。结果：致伤后第1天B、C、D、E组大鼠神经行为学评分及平衡能力评分均较致伤前及A组明显增加（P〈O．05）。伤后评分逐渐下降,B组大鼠得分除第1天外均低于c、D、E组（P〈0．05）,C、D、E组各时间点比较评分差异无统计学意义。致伤后B、C、D、E组爬坡角度均较致伤前及A组明显下降（P〈0．05）,B组大鼠在致伤第2天开始爬坡角度明显大于C、D、E组（P〈0．05）。结论：穴位注射胞二磷胆碱能明显促进脑创伤大鼠神经功能的恢复。     

Improvements in quantitative EEG following consumption of a natural citicoline-enhanced beverage

The present study examined the impact of a taurine-free drink enhanced with citicoline and other natural ingredients on electrophysiological markers of mental alertness. Ten healthy adult participants enrolled in a double-blind, placebo-controlled crossover study and were randomized to receive either placebo or the citicoline supplement on the first visit. Measures of electrical brain activity using electroencephalogram (EEG) were collected 30 min after consuming the beverage. Seven days after the initial assessment participants completed the alternative condition (placebo or citicoline beverage). Compared to placebo, significant improvements were found in frontal alpha EEG and N100 event related potentials (ERP) associated with the citicoline-enhanced supplement. These preliminary findings suggest that a novel brain drink containing compounds known to increase choline in the brain significantly improved attention as measured by ERP and EEG. These findings suggest that a viable and alternative brain supplement without potential compounds such as taurine may augment attentional mechanisms in healthy individuals.     

Cytidine-5'-diphosphocholine affects CTP-phosphocholine cytidylyltransferase and lyso-phosphatidylcholine after transient brain ischemia

Cytidine-5'-diphosphocholine (CDP-choline, also referred as citicoline), the key intermediate in phosphatidylcholine (PtdCho) synthesis, provided significant benefit in experimental central nervous system (CNS) injury including cerebral ischemia. CDP-choline is synthesized by CTP:phosphocholine cytidylyltransferase (CCT), the key rate-limiting enzyme in PtdCho synthesis. Phospholipase A(2) (PLA(2)) hydrolyzes PtdCho to produce free fatty acids and lyso-PtdCho, an inhibitor of CCT. We investigated the status of CCT and lyso-PtdCho after 10-min transient brain ischemia in gerbils with reperfusion up to 2 days. Ischemia with no reperfusion resulted in loss of CCT activity in cytosol (408 +/- 8 pmol/min/mg protein compared to sham 695 +/- 45; P < 0.01) and membrane (383 +/- 61 compared to sham 532 +/- 54; P < 0.05). CCT activity remained low over 24-hr reperfusion, and returned to sham levels at Day 2 in membrane but remained low in cytosol. CDP-choline significantly increased CCT activity in cytosol at 1 hr reperfusion (saline, 339 +/- 35 compared to CDP-choline, 430 +/- 70; P < 0.05) and in membrane at 6 hr (saline, 381 +/- 32 compared to CDP-choline, 489 +/- 50; P < 0.01) and 24 hr (saline, 417 +/- 24 compared to CDP-choline, 594 +/- 45; P < 0.01), but had no effect on CCT activity at Day 2. Lyso-PtdCho increased at 1-hr reperfusion (219 +/- 5 nmol/g tissue compared to sham, 92 +/- 8; P < 0.01), and remained elevated over 2 days. CDP-choline attenuated lyso-PtdCho levels at 1-hr reperfusion (162 +/- 21, P < 0.01 compared to saline). These data indicate that PtdCho synthesis is impaired after brain ischemia, and CDP-choline may increase PtdCho levels by attenuating the loss of CCT activity and lyso-PtdCho formation.     

1,6-二磷酸果糖治疗新生儿窒息并发缺氧缺血性脑病的研究

目的　观察 1,6-二磷酸果糖对新生儿窒息并发缺氧缺血性脑病 (HIE)的治疗作用。方法　将 2 0 2例HIE患儿随机分为 2组 ,治疗组给予 1,6-二磷酸果糖 ,对照组给予胞二磷胆碱治疗。结果　治疗组有效率为 96.2 % ,对照组有效率为 80 .6% ,两组比较有显著性差异(P <0 .0 5 )。结论　 1,6-二磷酸果糖治疗新生儿HIE有效率高 ,神经系统后遗症少 ,且无明显的副作用     

纳络酮、脑活素、胞二磷胆碱、尼莫地平治疗新生儿缺血缺氧性脑病的临床研究

【目的】研究新生儿缺氧缺血性脑病的治疗方法,为临床治疗提供参考依据。【方法】将302例患儿随机分为实验组和对照组。两组均在供氧、控制惊厥、减轻脑水肿、维持水电解质平衡等综合治疗的基础上,实验组先给予纳络酮,疗程3 d,第4 d应用脑活素、胞二磷胆碱治疗,疗程7~10 d;并于入院第1 d口服尼莫地平,疗程7 d。对照组给予脑活素、胞二磷胆碱治疗,疗程10~14 d。对两组患儿治疗后主要临床症状、体征恢复正常需要的时间,临床疗效及NBNA评分进行比较。【结果】实验组呼吸、肌张力、面色、哭声、心率、前囟张力、意识状态恢复正常需要的时间明显低于对照组。实验组临床效果明显优于对照组,治疗后实验组NBNA评分明显高与对照组,差异均有显著性(P均<0.01)。【结论】应用纳络酮、脑活素、胞二磷胆碱、尼莫地平治疗新生儿缺氧缺血性脑病效果显著。     

生脉注射液对缺氧缺血性脑病新生儿脑血流动力学影响

目的利用彩色多普勒超声研究缺氧缺血性脑病(HIE)新生儿脑血流动力学变化,观察生脉注射液对HIE新生儿脑血流动力学的影响,探索HIE早期治疗干预措施。方法将50例HIE患儿随机分为生脉组(30例)和胞磷胆碱组(20例),分别于治疗前及治疗1周后进行脑血流动力学参数。包括大脑中动脉和大脑前动脉的收缩期峰值流速(PSFV)、舒张末期血流速度(EDFV)、搏动指数(PI)、阻力指数(RI)检测,并与正常对照组进行对比研究。结果HIE患儿PSFV、PI及RI均低于正常对照组,差异均有显著性(P均<0.05);两组治疗后PSFV、EDFV、RI较治疗前明显提高(P<0.01,0.05),生脉组大脑前动脉血流速度的改善明显高于胞磷胆碱组(P<0.01,0.05)。结论HIE新生儿脑血流动力学出现明显紊乱,应用无损伤性彩色超声诊断仪监测脑血流动力学变化有助于早期评估其预后,并指导临床治疗;生脉注射液、胞磷胆碱治疗HIE均有效,但生脉注射液优于胞磷胆碱。