HPLC法测定复方醋酸地塞米松搽剂中醋酸地塞米松和樟脑的含量

目的采用HPLC法测定复方醋酸地塞米松搽剂中醋酸地塞米松和樟脑的含量。方法色谱柱为C18,流动相为甲醇-水（60：40,V／V）,检测波长醋酸地塞米松为240nm和樟脑为290am,流速1mL·min^-1,柱温为室温。结果醋酸地塞米松和樟脑分别在5．05～80．8mg·L^-1（r=0．9999）和0．1～5．0g·L^-1（r=0．9999）浓度内线性关系良好,平均回收率分别为100．6％（RSD=0．49％）和100．7％（RSD=0．61％）。结论本方法简便,准确性、重现性好,适用于复方醋酸地塞米松搽剂中醋酸地塞米松和樟脑的含量测定。     

毛细管气相色谱法测定复方麝香草酚搽剂的含量

目的建立气相色谱（GC）法同时测定复方麝香草酚搽剂中樟脑、麝香草酚含量的方法。方法采用DB-624弹性石英毛细管柱程序升温法。结果2组分能很好分离;樟脑、麝香草酚分别在0．2087～2．087、0．1140～1．140mg／ml浓度范围内呈良好线性关系;平均回收率分别为99．6％（RSD=0．9％）、99．6％（RSD=1．9％）。结论本方法准确、灵敏,可用于该制剂的质量控制。     

两种方法治疗玫瑰糠疹的临床疗效对比分析

目的观察泛昔洛韦与加用氯雷他定、复方青黛胶囊和复方樟脑乳膏联合两种治疗方法对玫瑰糠疹的临床疗效。方法 64例玫瑰糠疹患者随机分为单用泛昔洛韦治疗32例(对照组)和加用氯雷他定、复方青黛胶囊和复方樟脑乳膏联合治疗32例(实验组)。对照组口服泛昔洛韦,每次0.3克,2次/天。实验组在对照组的基础上口服氯雷他定,每次10mg,1次/天;口服复方青黛胶囊,一次4粒,3次/天;复方樟脑乳膏搽患处,3次/天。两组均以7天为一个疗程,14天后观察比较临床疗效。结果治疗后两组的疗效比较有显著统计学差异(P<0.01),实验组患者的疗效明显好于对照组;实验组患者未出现皮肤刺痒等并发症及明显的副作用。结论泛昔洛韦、氯雷他定、复方青黛胶囊和复方樟脑乳膏联合治疗玫瑰糠疹疗效显著且安全、无明显毒副作用。     

Distribution of Volatiles in Artemisia cana.

Abstract

The chemical composition of essential oils isolated from aerial parts of wild sage, Artemisia cana. Pursh (Asteraceae), was investigated. Analyses were performed by GC and GC/MS using two columns of different polarities, polyethylene glycol (DB-Wax) and 5% phenyl 95% polydimethylsiloxane (HP-5), which enabled the separation of several co-eluting components. Up to 2.3% of oil (w/w, dry basis) was obtained by hydrodistillation of aerial parts, excluding leafless woody stalks. The stalks of Artemisia. species contained insignificant amounts of essential oils. Flowers, leaves, and stalks yielded 2.8%, 2.4%, and 0.1% of oil, respectively. A total of 34 components were identified accounting for 92.6–87.6% of the oils. The chemical composition of the oils extracted from different plant parts was very similar. High contents of 1,8-cineole (20.8–11.0%) and camphor (55.2–37.4%) were found in Artemisia cana. oils. For pilot-scale testing purposes, aerial parts were separated from leafless woody stalks and processed in a 10L DIG-Maz extractor. The chemical composition of the recovered product was similar to that of the lab-distilled oil. The oil was characterized by a light yellow to green liquid with an herbaceous, balsamic, and fresh-camphoraceous odor.     

Improving photoprotection: 4-methylbenzylidene camphor microspheres

Abstract

We propose a new approach for photoprotection. 4-Methylbenzylidene camphor (4-MBC), one of the most widely used UV filters, was encapsulated in microspheres, with a view to overcoming problems (percutaneous absorption, photodegradation and lack of lasting effect) arising with organic sunscreens, and to achieve safe photoprotection. We focused on this filter in the light of the Cosmetics Europe opinion concerning its possible effects on the thyroid gland. Microspheres were prepared by emulsification–solvent evaporation, using different amounts of 4-MBC and characterized for morphology, encapsulation efficiency and particle size. The particles were then mixed in O/W emulsions. The in vitro sun protection factors, in vitro release and photostability were investigated and compared with emulsions containing the free sunscreen. The new microspheres offer good morphology and loading (up to 40%), and the same photoprotection as the free filter while at the same time protecting it from photodegradation. The systems also give a slower release from the emulsions.     

樟树挥发油的提取及包合物制备与鉴定研究

目的比较湖南樟树老叶、嫩叶、落叶、籽中挥发油含量差异,制备樟树挥发油的β-环瑚精包合物并对其进行鉴定。方法采用水蒸气蒸馏法提取挥发油;以挥发油包合率为评价指标,对研磨法、饱和水溶液法、超声法进行比较,并采用正交试验法优选研磨法的最佳包合工艺条件;通过薄层色谱法、紫外可见吸收光谱法、差示扫描量热法对包合物进行鉴定。结果 6-7月的样本中,樟树挥发油的平均得率分别为老叶1.58%、嫩叶1.52%、落叶0.84%、籽1.39%。优选的包合方法为研磨法,最佳包合工艺为β-环瑚精与挥发油的投料比为10∶1,加4倍量水,研磨2 h。樟树挥发油包合前后的薄层色谱、紫外可见吸收图谱均无差异;包合物、β-环瑚精、挥发油、β-环瑚精与油物理混合物的热分析图谱有显著差异。结论樟树挥发油的含量以老叶较高;本试验优选的包合工艺合理、稳定。     

冰片对黄芩苷角膜透过性的影响

目的研究冰片对黄芩苷通过离体角膜的影响,考察其与黄芩苷联合用药的可行性。方法采用改良的立式Franz扩散池,以林格氏液为扩散介质考察在不同浓度冰片的促进渗透下黄芩苷的离体兔角膜通透性。结果质量分数0.01%、0.03%、0.05%、0.08%和0.1%冰片分别使黄芩苷的渗透增强比为3.86、11.0、14.27、21.27和36.55,与黄芩苷对照组比较呈显著性差异(P<0.01)且质量分数0.1%冰片渗透效果最佳。结论冰片对黄芩苷通过离体角膜有显著的促进作用,具有良好的开发应用前景。     

中药六神丸+樟脑酚间接无痛失活乳牙的临床应用

目的:观察中药六神丸 樟脑酚在不穿髓情况下无痛失活乳牙的临床效果. 方法:选择未露髓的急慢性牙髓炎乳磨牙280例,用六神丸 樟脑酚无痛法间接失活乳牙与常规使用乳牙失活剂开髓后封药失活乳牙对照研究,比较其优缺点. 结果:使用中药六神丸 樟脑酚无痛法间接失活乳牙牙髓安全有效,痛苦少,成功率为90.1%,略低于使用乳牙失活剂失活. 结论:使用中药六神丸 樟脑酚无痛法间接失活乳牙牙髓有较高的成功率,操作简便,且较开髓后封乳牙失活剂失活痛苦少,更容易被患儿接受,具有一定临床实用价值.     

气相色谱法同时测定祛伤消肿酊中樟脑、薄荷脑、龙脑的含量

目的 建立祛伤消肿酊中同时测定樟脑、薄荷脑及龙脑含量的方法.方法 采用气相色谱法,FID检测器.色谱柱:HP- INNOWAX(30m×0.32mm×0.25μm);柱温:130℃;进样口温度:250℃;检测器温度:300℃.结果 樟脑、龙脑、薄荷脑回归方程的相关系数均在0.999以上,平均回收率(n=6)分别为97....     

GC法同时测定麝香追风膏中7个挥发性成分的含量

目的建立同步测定麝香追风膏中桉油精、樟脑、薄荷脑、冰片(龙脑和异龙脑)、水杨酸甲酯、桂皮醛和丁香酚等7种成分含量的气相色谱方法。方法采用气相色谱法,HP-INNOWAX毛细管色谱柱,氮气为载气,FID检测器,升温程序为80℃保持5 min,60℃/min升温至150℃保持5 min,80℃/min升温至200℃保持2 min,内标法测定样品中7种成分的含量。结果麝香追风膏中7种成分及内标物萘在同一色谱条件下获得良好分离。回收率分别为96.23%(RSD=2.48%)、100.31%(RSD=0.23%)、96.76%(RSD=0.76%)、96.90%(RSD=1.39%)、98.52%(RSD=1.57%)、96.32%(RSD=2.01%)和103.31%(RSD=2.12%)。采用此法对3批样品进行含量测定,均取得了满意的结果。结论该方法简便、准确,分离度好,可用于控制麝香追风膏的质量。