高效液相色谱法测定通脉降糖颗粒中盐酸小檗碱的含量

目的 :测定通脉降糖颗粒中盐酸小檗碱的含量。方法 :高效液相色谱法。SpherigelC1 8柱 ,乙腈 水 (55∶35)为流动相 ,每 1 0 0 0ml流动相中含磷酸二氢钾 3 .4g,十二烷基硫酸钠 1 .7g ,流速 1ml·min- 1 ,检测波长 345nm。结果 :通脉降糖颗粒中盐酸小檗碱与多种杂质能有效分离。盐酸小檗碱进样量在 0 .0 2 65～ 0 .2 1 2 0 μg范围内线性关系良好 ,γ =0 .9998。平均回收率为 1 0 1 .6 % (n =5 ,RSD =1 .8% )。结论 :本方法简便、快速、准确     

介质环境对小檗碱荧光光谱影响的研究

探讨介质环境对小檗碱荧光光谱性质的影响。方法配制在不同溶剂及ＰＨ条件下的２＊１０＾－５ｍｏｌ．Ｌ＾－１小檗碱溶液，测定灾光光谱。结果：随着溶剂极性的降低，其荧光发射光谱紫移并且荧光强度显著提高。结论小檗碱所具有的特殊荧光性质，可作为生物荧光探针的优选试剂。     

小檗碱对非酒精性脂肪性肝病患者血清骨钙素的影响

目的:探讨小檗碱对伴糖代谢紊乱的非酒精性脂肪性肝病(NAFLD)患者的血清未羧化骨钙素(ucOC)水平的影响。方法:对伴有糖耐量受损或2型糖尿病的114例NAFLD患者,进行生活方式干预,其中57例(小檗碱组)加小檗碱治疗(0.5 g/次,每日3次),共干预16周,其余57例仅进行生活方式干预,作为对照组。评价干预前后患者ucOC水平变化及血糖、血脂和肝脏脂肪含量(HFC)等指标,分析ucOC变化的影响因素。结果:与对照组相比,小檗碱组患者血清ucOC水平升高更明显(P0.001)。校正年龄、性别和体质指数(BMI)后,小檗碱组血清ucOC的改善情况[4.60(3.50, 5.70)]仍明显优于对照组[0.96(-0.14, 2.05),P0.001],平均差异为3.64 (95%CI 1.74～5.55)。亚组分析显示,在非肥胖老年男性患者中,小檗碱升高ucOC的作用更加明显。偏相关分析显示,在校正年龄、性别后,治疗前后血清ucOC水平差值与BMI、体质量、腰围、HFC、糖化血红蛋白(HbA1c)、总胆固醇(TC)的差值负相关(P0.05)。结论:小檗碱能改善伴糖代谢紊乱的非酒精性脂肪性肝病患者代谢异常,可能与其升高血清ucOC水平有关。     

Berberine and its structural analogs have differing effects on functional profiles of individual gut microbiomes

ABSTRACT

The understanding of the effects of compounds on the gut microbiome is limited. In particular, it is unclear whether structurally similar compounds would have similar or distinct effects on the gut microbiome. Here, we selected berberine (BBR), an isoquinoline quaternary alkaloid, and 16 structural analogs and evaluated their effects on seven individual gut microbiomes cultured in vitro. The responses of the individual microbiomes were evaluated by metaproteomic profiles and by assessing butyrate production. We show that both interindividual differences and compound treatments significantly contributed to the variance of metaproteomic profiles. BBR and eight analogs led to changes in proteins involved in microbial defense and stress responses and enrichment of proteins from Verrucomicrobia, Proteobacteria, and Bacteroidetes phyla. It also led to a decrease in proteins from the Firmicutes phylum and its Clostridiales order which correlated to decrease proteins involved in the butyrate production pathway and butyrate concentration. Three of the compounds, sanguinarine, chelerythrine, and ethoxysanguinarine, activated bacterial protective mechanisms, enriched Proteobacteria, increased opacity proteins, and markedly reduced butyrate production. Dihydroberberine had a similar function to BBR in enriching the Akkermansia genus. In addition, it showed less overall adverse impacts on the functionality of the gut microbiome, including a better maintenance of the butyrate level. Our study shows that ex vivo microbiome assay can assess differential regulating effects of compounds with subtle differences and reveals that compound analogs can have distinct effects on the microbiome.     

健脾康儿片的质量控制研究

目的:进行健脾康儿片的质量控制研究。方法:采用反相高效液相色谱法对健脾康儿片中主要成分盐酸小檗碱进行定量分析;同时对制剂中的主要药味人参、黄连、陈皮进行薄层鉴别。结果:用高效液相色谱法测定盐酸小檗碱的含量,在0.054～0.648μg,盐酸小檗碱峰面积值与进样量有良好的线性关系(r=0.999 6)。平均加样回收率97.5%,RSD 1.51%(n=6),薄层图谱斑点清晰,阴性对照无干扰。结论:方法简便,重复性好,可有效控制制剂的质量。     

HPLC测定消炎栓中黄芩苷、盐酸小檗碱的含量

目的 采用高效液相色谱法同时测定消炎栓中黄芩苷和盐酸小檗碱的含量,以提高其质量标准.方法 Waters SymmetryShield-C18柱(4.6 mm× 250 mm,5 μm)为固定相,以乙腈-0.4％磷酸溶液进行梯度洗脱,流速为1.0 mL/min,检测波长为264nm.结果 黄芩苷、盐酸小檗碱的线性范围分别...     

高效液相色谱法测定二妙丸中盐酸小檗碱的含量

目的建立二妙丸中盐酸小檗碱的含量测定方法。方法采用高效液相色谱法,DiamonsilODSlC18柱,以乙腈-0．1％磷酸溶液（50：50,每100mL加十二烷基磺酸钠0．1g）为流动相,流速为1．0mL／min,检测波长265nm。结果盐酸小檗碱在0．1386～2．772μg范围内呈良好线性,回归方程为,：2572．354X＋79．851,r=0．9997。平均加样回收率为98．67％,RSD=1．26％（n=6）。结论方法简便、准确,专属性强,测定结果重复性好,为二妙丸中盐酸小檗碱的定量分析提供了科学有效的方法。     

HPLC测定岩黄连生物总碱中脱氢卡维丁、盐酸巴马汀和盐酸小檗碱

目的:建立高效液相色谱法测定岩黄连生物总碱中脱氢卡维丁、盐酸巴马汀及盐酸小檗碱含量的方法,为完善岩黄连生物总碱的质量评价提供依据。方法:采用高效液相色谱法,以Agilent XDB C18(4.6 mm×150 mm,5μm)为色谱柱,以乙腈-0.01 mol.L-1磷酸二氢钾水溶液(18.5∶81.5)为流动相,流速1.0 mL.min-1,柱温30℃,检测波长347 nm。结果:脱氢卡维丁进样量在15.375～92.25 mg.L-1(r=0.999 9)呈良好的线性关系,平均加样回收率96.43%,RSD 1.13%;盐酸巴马汀进样量在14.91～89.46 g.L-1(r=0.999 9)呈良好的线性关系,平均加样回收率96.28%,RSD 1.22%;盐酸小檗碱进样量在3.525～28.2 mg.L-1(r=0.999 7)呈良好的线性关系,平均加样回收率为103.02%,RSD 1.35%。结论:所建立的方法简便、准确、重复性好,可以用于控制岩黄连生物总碱的质量。     

高效毛细管电泳法测定三棵针中3种生物碱的含量

目的:建立毛细管区带电泳同时测定不同产地三棵针中盐酸小檗碱、盐酸药根碱、盐酸巴马汀含量的方法。方法:空心石英毛细管(75 cm×50μm)柱,以50 mmol.L-1硼砂-甲醇(2∶1)的溶液为电泳介质,运行电压25 kV,柱温15℃,检测波长230 nm。结果:盐酸小檗碱、盐酸药根碱及盐酸巴马汀分别在0.816 8～32.672 0 mg.L-1(r2=0.999 4),0.852 0～8.520 0 mg.L-1(r2=0.999 5),0.815 2～8.152 0 mg.L-1(r2=0.999 6)线性关系良好;其平均加样回收率分别为103.8%(RSD 1.14%),100.7%(RSD 3.46%),100.2%(RSD 2.87%)。结论:实验方法快速简便,准确可靠,适用于三棵针等药材中盐酸小檗碱、盐酸巴马汀及盐酸药根碱的含量测定。