透明质酸钠关节内注射治疗膝骨性关节炎

目的　观察透明质酸钠注射液对膝骨关节炎的疗效。方法　关节内注射透明质酸钠 2ml,每周一次 ,5周一疗程 ,疗程结束后连续随诊一年半以上。结果　疗程结束时总有效率为 92 7% ,随访过程 82 %患者无疼痛、肿胀、渗出等症状复发。结论　透明质酸钠可有效地抑制膝骨关节炎患者的肿胀、渗出、疼痛等症状     

自体骨膜游离移植修复关节软骨缺损进展

探讨自体骨膜游离移植修复关节软骨缺损的研究进展。从骨膜的解剖生理功能和骨膜再生软骨能力两方面进行分析,并阐述了关节内营养环境、关节活动、骨膜生发层不同朝向、年龄及手术技巧等因素对移植修复的影响。同时总结了该方法的临床应用状况、效果及存在的问题。实验及临床的研究证明自体骨膜游离移植是修复关节软骨缺损的重要手段,局部环境因素影响骨膜成软骨。     

带血管蒂髌骨移位修复膝关节面缺损的应用解剖

目的：研究用带血管蒂髌骨移位的方法修复股骨髁和胫髁关节面缺损。方法：用37例经动脉红色乳胶灌注的下肢标本,经解剖、厚切片和铸型研究髌骨的血供。观察30块髌骨和胫骨内外侧髁关节面的形态和面积。结果：供应髌骨的各支动脉在先在髌骨周围吻合成髌周动脉环,再由该环发支进入髌骨;参与形成髌周动脉环的主要动脉有膝上外动脉、膝下外动脉、膝降动上支,膝降动脉髌下支和膝下内动脉;髌骨关节面与股骨内外侧髁下关节面、胫骨内外侧形态大小相似。结论：带血管蒂骨可以移位至股骨内外侧髁、或胫骨内外侧髁,利用髌骨的关节面修复股骨髁或胫骨髁关节面缺损,以期最大限度地保留膝关节的功能。     

Three rare cases of benign mixed tumour of the skin displaying mature bone and cartilage elements

Mixed tumour of the skin is a rare adnexal tumour of sweat gland origin, which can be benign or malignant. Histologically, it is defined by the presence of epithelial, myoepithelial and mesenchymal elements. The latter can take several forms, but both bone formation and cartilage formation are independently rare features. We present three rare cases of mixed tumour of the skin. Two of these displayed both mature bone and cartilaginous mesenchymal elements, while the third displayed mature bone formation without cartilage.     

软骨组织工程种子细胞的基础和应用研究进展

软骨种子细胞的老化和缺乏是限制软骨组织工程在临床应用的瓶颈问题。本文概述了目前对软骨种子细胞研究的两个方面 :扩大种子细胞的来源及预防和延缓种子细胞功能老化 ,并展望软骨组织工程在监床的应用前景。     

Design Characteristics for the Tissue Engineering of Cartilaginous Tissues

Tissues like the temporomandibular joint (TMJ) disc and the knee meniscus are often mistakenly viewed as a tantamount to hyaline cartilage, largely due to the absence of a comprehensive understanding of the distinguishing properties of cartilaginous tissues. Because of this confusion, fibrocartilaginous tissue engineering attempts may not be based on suitable experimental designs. Fibrocartilaginous tissues are markedly different than hyaline cartilage; however, the dearth of knowledge related to their cellular and biochemical composition, as well as their biomechanical characteristics, is stunning. Hyaline articular cartilage is exclusively composed of chondrocytes that produce primarily type II collagen, whereas the TMJ disc and the knee meniscus have a mixed cell population of fibroblasts and cells similar to chondrocytes, which predominantly secrete type I collagen. Additionally, fibrocartilaginous tissues have a low glycosaminoglycan content, a low compressive modulus, and a high tensile modulus when compared to hyaline cartilage. Therefore, it is crucial for fibrocartilaginous tissue engineering attempts to be tissue-specific, utilizing the knowledge of the distinct and unique properties of these tissues. At the same time, advances and insights related to the science and engineering aspect of hyaline cartilage regeneration must be carefully considered for the in vitro engineering of fibrocartilaginous tissues.     

兔膝关节软骨下自体骨移植后不同固定时间对软骨的影响

目的：研究兔膝关节软骨下自体骨转移后不同固定时间对关节软骨的影响。方法：健康大耳白兔５４只按术后膝关节不同固定时间随机分为Ａ、Ｂ、Ｃ３组。建立膝关节软骨下自体骨移植的动物模型,于术后８周取材,采用光镜、透射电镜对关节软骨进行形态学观察。结果：Ａ组２４％的关节软骨出现退变;Ｂ组１３％的关节软骨出现退变;Ｃ组２０．３％关节软骨出现退变。Ｂ组与Ａ组、Ｃ组相比,有显著性差异。结论：术后合理负重可降低关节软     

鲨鱼软骨制剂对三种细胞系的抑制效应

目的：研究鲨鱼软骨制剂（ＳＣＰ）对人胃癌细胞（ＭＧＣ８０－３）、红白血病细胞Ｋ５６２）、人脐静脉血管内皮细胞（ＶＥＣ３４０）的生长抑制作用,探讨其抗癌作用机制。方法：采用盐酸胍抽提、丙酮分级沉淀、烘干、微孔滤膜过滤等步骤从鲨鱼软骨粉中提取鲨鱼软骨制剂;采用ＭＴＴ法检测不同浓度ＳＣＰ对体外培养的ＭＧＣ８０－３细胞系、Ｋ５６２细胞系和ＶＥＣ３４０细胞系的生长抑制作用。结果：ＳＣＰ明显抑制ＭＧＣ８０－３