Three rare cases of benign mixed tumour of the skin displaying mature bone and cartilage elements

Mixed tumour of the skin is a rare adnexal tumour of sweat gland origin, which can be benign or malignant. Histologically, it is defined by the presence of epithelial, myoepithelial and mesenchymal elements. The latter can take several forms, but both bone formation and cartilage formation are independently rare features. We present three rare cases of mixed tumour of the skin. Two of these displayed both mature bone and cartilaginous mesenchymal elements, while the third displayed mature bone formation without cartilage.     

软骨组织工程种子细胞的基础和应用研究进展

软骨种子细胞的老化和缺乏是限制软骨组织工程在临床应用的瓶颈问题。本文概述了目前对软骨种子细胞研究的两个方面 :扩大种子细胞的来源及预防和延缓种子细胞功能老化 ,并展望软骨组织工程在监床的应用前景。     

Design Characteristics for the Tissue Engineering of Cartilaginous Tissues

Tissues like the temporomandibular joint (TMJ) disc and the knee meniscus are often mistakenly viewed as a tantamount to hyaline cartilage, largely due to the absence of a comprehensive understanding of the distinguishing properties of cartilaginous tissues. Because of this confusion, fibrocartilaginous tissue engineering attempts may not be based on suitable experimental designs. Fibrocartilaginous tissues are markedly different than hyaline cartilage; however, the dearth of knowledge related to their cellular and biochemical composition, as well as their biomechanical characteristics, is stunning. Hyaline articular cartilage is exclusively composed of chondrocytes that produce primarily type II collagen, whereas the TMJ disc and the knee meniscus have a mixed cell population of fibroblasts and cells similar to chondrocytes, which predominantly secrete type I collagen. Additionally, fibrocartilaginous tissues have a low glycosaminoglycan content, a low compressive modulus, and a high tensile modulus when compared to hyaline cartilage. Therefore, it is crucial for fibrocartilaginous tissue engineering attempts to be tissue-specific, utilizing the knowledge of the distinct and unique properties of these tissues. At the same time, advances and insights related to the science and engineering aspect of hyaline cartilage regeneration must be carefully considered for the in vitro engineering of fibrocartilaginous tissues.     

兔膝关节软骨下自体骨移植后不同固定时间对软骨的影响

目的：研究兔膝关节软骨下自体骨转移后不同固定时间对关节软骨的影响。方法：健康大耳白兔５４只按术后膝关节不同固定时间随机分为Ａ、Ｂ、Ｃ３组。建立膝关节软骨下自体骨移植的动物模型,于术后８周取材,采用光镜、透射电镜对关节软骨进行形态学观察。结果：Ａ组２４％的关节软骨出现退变;Ｂ组１３％的关节软骨出现退变;Ｃ组２０．３％关节软骨出现退变。Ｂ组与Ａ组、Ｃ组相比,有显著性差异。结论：术后合理负重可降低关节软     

鲨鱼软骨制剂对三种细胞系的抑制效应

目的：研究鲨鱼软骨制剂（ＳＣＰ）对人胃癌细胞（ＭＧＣ８０－３）、红白血病细胞Ｋ５６２）、人脐静脉血管内皮细胞（ＶＥＣ３４０）的生长抑制作用,探讨其抗癌作用机制。方法：采用盐酸胍抽提、丙酮分级沉淀、烘干、微孔滤膜过滤等步骤从鲨鱼软骨粉中提取鲨鱼软骨制剂;采用ＭＴＴ法检测不同浓度ＳＣＰ对体外培养的ＭＧＣ８０－３细胞系、Ｋ５６２细胞系和ＶＥＣ３４０细胞系的生长抑制作用。结果：ＳＣＰ明显抑制ＭＧＣ８０－３     

从人软骨提取总RNA和CDMP－1成熟肽片段的克隆

目的 克隆人软骨来源形态发生蛋白－１（ＣＤＭＰ－１）成熟肽编码区的基因片段。方法 用异流氰酸胍一步法从人软骨组织和酶消化法原代培养的软骨细胞中分别抽提总ＲＮＡ,利用ＲＴ－ＰＣＲ方法扩增出人ＣＤＭＰ－１成熟区的基因片段（约１．０ｋｂｐ）,将所得基因片段插入ｐＢｌｕｅｓｃｒｉｐｔ）质粒载体,重组质粒ＤＮＡ,通过酶切分析和核苷酸序列分析鉴定阳性克隆。结果 从罗骨组织直接取到总ＲＮＡ,其质量与从等重量软骨     

雷公藤单体T4对IL-1刺激引起的类风湿患者关节滑膜成纤维细胞增殖和产生IL-6的影响

目的　探讨雷公藤单体 T4对经 IL- 1刺激的体外培养的类风湿关节炎 (RA)患者的关节滑膜成纤维细胞增殖和产生 IL- 6的影响。方法　关节滑膜组织来自行关节置换术或滑膜切除术的类风湿患者。滑膜组织经体外消化使细胞分散后 ,传代培养。以培养 3代后的滑膜成纤维细胞为对象 ,先给予 IL- 1刺激 ,再加入雷公藤 T4单体。结果　 T4可抑制由 IL- 1刺激的滑膜成纤维增殖 ,但不影响 IL- 6的产生。结论　 T4的上述作用可能是其治疗RA的机制之一 ,本研究结果为 T4早日应用于临床治疗 RA提供了一定的理论依据     

带蒂皮瓣修复关节损伤的实验研究

目的：将家兔带蒂皮瓣移植于破坏掉关节软骨的股骨头上,探讨皮肤成纤维细胞的成软骨潜能。方法：用家兔２０只,每只任选一侧股骨头作为实验组,对侧作为对照组。将带蒂皮瓣移植于破坏掉了关节软骨的股骨头上后,将股骨头复位,作为实验组。对侧则以游离全厚皮片作对照。分别在术后８ｗｋ、１６ｗｋ时各探查１０只动物,通过肉眼观察,光镜观察移植物的转归。结果：实验组皮瓣成活并以骨头愈合,表皮逐渐变性消失,真皮纤维组织增生