兔主动脉内皮细胞制备方法的优化

目的为构建骨组织工程血管化提供优质种子细胞。方法采用血管内膜外翻和胶原酶消化法分离培养兔主动脉内皮细胞并传代;采用倒置显微镜、透射电镜观察和免疫荧光法鉴定。结果培养的血管内皮细胞在第3~5天为对数生长期,倒置显微镜下可见内皮细胞呈紧密排列、短梭多角形的"鹅卵石"形态;透射电镜可见内皮细胞特征性Weible-Palade小体;经免疫荧光法鉴定证明该细胞CD31抗原阳性。结论采用血管内膜外翻等方法制备的兔血管内皮细胞的成活率高,且纯度高、数量多、具有良好的生物学活性。该法可为骨组织工程血管化的研究提供种子细胞。     

The dynamic fetal brain

PURPOSE: To evaluate fetuses with normal intracranial anatomy in the second trimester that became abnormal in the third trimester. METHODS: We sonographically examined 6 fetuses with a normal second-trimester head sonogram that presented later in pregnancy with an abnormal head sonogram. RESULTS: Four categories of intracranial pathology were depicted: obstructive hydrocephalus, intraventricular intracranial hemorrhage, non-intraventricular intracranial hemorrhage, and porencephaly. CONCLUSIONS: Despite a normal midtrimester intracranial examination, evaluation of the fetal intracranial contents should be undertaken in subsequent sonographic examinations, because significant pathology can develop spontaneously.     

先天性二叶主动脉瓣畸形27例临床分析

27例住院治疗的先天性二叶主动脉瓣(CBAV)临床分析,结果显示:合并心功能不全21例(78%),合并感染性心内膜炎(IE)12例(44%),与其它心血管畸形并存7例(26%).IE为CBAV的主要死亡原因.本文认为,并发IE或存在明显血流动力学障碍时外科换瓣术为CBAV治疗的根本措施,结果还提示,CBAV一旦心功能失代偿发展迅速,准确掌握外科治疗指征及尽早手术至关重要.     

尿道下裂术后尿道狭窄的尿流率特点

目的 研究尿道下裂术后尿道狭窄的尿流率特点,为尿道狭窄的诊断提供客观依据.方法 采用Dantec尿动力学检验仪,对10例尿道下裂术后尿道狭窄的患者进行手术矫治前、后的尿流率测定,分析其尿流率特点.并选择10例正常男性作为对照,进行对比观察.结果 10例尿道下裂术后尿道狭窄患者的尿流率曲线明显低平,最大尿流率降低,排尿时间显著延长.尿道狭窄矫治术后,患者的最大尿流率得到提高,排尿时间缩短,其中3例患者的尿流率曲线接近正常.结论 尿道下裂术后尿道狭窄的尿流率曲线为特征性的低平曲线,尿流率明显降低.因此,尿流率测定可以作为尿道下裂术后尿道狭窄的诊断依据.     

Preliminary results of Zenith Fenestrated abdominal aortic aneurysm endovascular grafts

Background

Patients with juxtarenal aortic aneurysms who are unfit for open repair may be considered for fenestrated endovascular repair (fenEVAR). We report our initial experience with fenEVAR.

Methods

We reviewed the data on all our patients receiving fenEVAR for juxtarenal aortic aneurysms.

Results

Eight patients, average age 75 years, underwent fenEVAR. Endografts were designed from details obtained from preoperative computed tomography angiography. There were 6 grafts with superior mesenteric scallops and bilateral renal fenestrations, 1 with bilateral renal scallops, and 1 with a single renal fenestration. All patients survived 30 days. There was no renal failure requiring dialysis. At 10 weeks, 1 patient died from acute intestinal ischemia and multisystem organ failure, and another died from respiratory failure.

Conclusions

It is feasible to offer fenEVAR to patients who are poor candidates for open repair. However, these procedures are technically challenging. Early outcomes are less favorable than other aortic endovascular procedures.