三磷酸腺苷生物荧光液闪测定法检测活细胞计数

为建立一种检测活细胞数的方法,作者采用三磷酸腺苷(ATP)生物荧光液闪测定法检测小鼠纤维瘤细胞株L929.ATP标准曲线工作范围为10 -9～10-5mol/ml,相关系数r=0.9963(P＜0.001);当活细胞数在3×1 02～106个/ml时与发光计数值有良好的线性关系,r=0.9922(P＜0.001),变异系数 CV = 1%～3%.用ATP生物荧光液闪测定法检测活细胞数灵敏、简便、准确、重复性好,用液闪计数仪即可完成测定,有较大的实用价值.     

脐血造血干细胞移植对肿瘤化疗病人骨髓功能的影响

1目的　探讨脐血造血干细胞移植对因化疗所致骨髓功能抑制的恶性肿瘤病人骨髓造血功能重建的临床意义。 2方法　从健康人脐血中纯化制备造血干细胞 ,每例病人每天静脉输注 2× 10 9个造血干细胞 ,连续5 d.以治疗前后自身对照进行疗效评价。 3结果　治疗前白细胞计数为 (2 .78± 1.72 )× 10 9/ L,治疗后第 7天为(4.5 5± 1.0 1)× 10 9/ L,两组比较有显著性差异 (F=18.5 0 ,q=6 .95 ,P<0 .0 1) ;治疗前血红蛋白含量为 (12 1.5 6±11.2 7) g/ L,治疗后第 14天为 (130 .72± 12 .5 8) g/ L,治疗前后比较差异有极显著意义 (F=14.0 2 ,q=4.90 ,P<0 .0 1) ,且治疗后第 2 1天比治疗后第 7天增高 (F=14.0 2 ,q=6 .2 6 ,P<0 .0 1) ;治疗前血小板计数为 (192 .38±18.2 1)× 10 9/ L,治疗后第 2 1天为 (2 0 1.5 7± 17.86 )× 10 9/ L,治疗前后比较差异有极显著意义 (F=9.0 1,q=3.99,P<0 .0 1)。 4结论　脐血造血干细胞移植对于重建或改善骨髓造血功能有一定疗效。提升白细胞速度较血红蛋白及血小板为快。     

ITP患者骨髓及血液检测指标与疗效关系的探讨

目的：为观察血小板表面相关抗体Ｇ（ＰａＩｇＧ）、外周血血小板计数（ＢＰＣ）、平均血小板容积（ＭＰＶ）、骨髓巨核细胞计数（ＭＫ）与产板型巨核细胞比例（ＰＰＭＫ）对特发性血小板减少性紫癜（ｉｄｉｏｐａｔｈｉｃ ｔｈｒｏｍｂｏｃｙｔｏｐｅｎｉｃｐｕｒｐｕｒａ,ＩＴＰ）患者疗效的预计价值。方法：采用Ｃｏｕｌｔｅｒ全自动血球分析仪及双抗体夹心ＥＬＩＳＡ法等,对７３例ＩＴＰ患者治疗前和治疗后的ＢＰＣ、ＭＰＶ、     

Mendelian randomization suggests a potential causal effect of eosinophil count on influenza vaccination responsiveness

Currently, the clinical factors affecting immune responses to influenza vaccines have not been systematically explored. The mechanism of low responsiveness to influenza vaccination (LRIV) is complicated and not thoroughly elucidated. Thus, we integrate our in-house genome-wide association studies (GWAS) analysis result of LRIV (N = 111, Ncase [Low Responders] = 34, Ncontrol [Responders] = 77) with the GWAS summary of 10 blood-based biomarkers (sample size ranging from 62 076−108 794) deposited in BioBank Japan (BBJ) to comprehensively explore the shared genetics between LRIV and blood-based biomarkers to investigate the causal relationships between blood-based biomarkers and LRIV by Mendelian randomization (MR). The applications of four MR approaches (inverse-variance-weighted [IVW], weighted median, weighted mode, and generalized summary-data-based MR [GSMR]) suggested that the genetically instrumented LRIV was associated with decreased eosinophil count (β = −5.517 to −4.422, p = 0.004−0.039). Finally, we conclude that the low level of eosinophil count is a suggestive risk factor for LRIV.     

The predictive value of basal follicle stimulating and growth hormone levels as determined by immunofluorometry during assisted reproduction

Purpose Our purpose was (a) to investigate relationships of baseline endocrine serum levels with selected assisted reproduction (AR) parameters and (b) to evaluate the clinical applicability of an immunofluorometric assay (IFMA) as an alternative to a radioimmunoassay (RIA).Methods Basal endocrine values (analyzed by RIA—rFSH, rLH, and rE₂; analyzed by IFMA—fFSH, fLH, and fGH) were determined for female patients (n = 142) preceding ovarian stimulation for AR.Results Specific AR parameters correlated significantly with RIA- and IFMA-determined FSH levels, although IFMA correlations consistently exceeds that of RIA. Cutoff values of fFSH 11.68 IU/L or rFSH 15.0 IU/L indicated a poor response. The high-basal fFSH group was older (34 vs 31 years; P = 0.0334) and yielded fewer oocytes (2.9 vs 4.6 oocytes; P = 0.0018) than the low-basal fFSH group (<11.68 IU/L). Lower cumulative embryo scores and conception rates were also associated with the high-fFSH group, compared to the low-fFSH group.Conclusions This study confirms the negative impact of elevated basal FSH levels on AR. Basal LH, GH, and E₂ levels are, in comparison to baseline FSH levels, unsuitable indices for estimating ovarian responsiveness. IFMA can be regarded as an alternative to RIA in both clinical and research laboratories.     

Ovarian stimulation in women undergoing in-vitro fertilization and embryo transfer using recombinant human follicle stimulating hormone (Gonal-F) in non-down-regulated cycles

In order to assess the efficacy and safety of recombinant humanfollicle stimulating hormone (FSH) in routine clinical use,ovarian stimulation with recombinant human FSH was performedin 71 patients prior to in-vitro fertilization (IVF) withoutgonadotrophin-releasing hormone (GnRH) analogues in a multicentre,non-comparative study. Human chorionic gonadotrophin (HCG) wasadministered to 58 patients (81.7%), 15 of whom underwent 19cycles with an initial dosage of three ampoules daily of recombinantFSH and 43 of whom underwent 152 cycles with four ampoules dailyfrom day 3 onwards. No significant differences were detectedbetween these two groups in all test parameters. The mean durationof treatment was 9.06 and 8.86 days respectively with a meannumber of 24.06 and 23.25 vials of recombinant human FSH administered.A mean number of 6.26 and 5.88 oocytes respectively was collected.The number of transferred embryos was 2.4 and 2.2. A clinicalpregnancy rate of 23.8% (10 out of 42) per transfer was achieved(30.9 and 20.6% respectively). Local tolerance of s.c. administrationwas excellent. Mild pain at the injection site was the dominantfinding in <20% of patients. Two cases of ovarian hyperstimulationsyndrome were noted. Recombinant human FSH is very attractiveto patients because it can be self-administered s.c. and thepreparation does not come from a human source. In conclusion,these data support the safety and efficacy of recombinant humanFSH in routine use for IVF.     

武汉市旅店业卫生间三大卫生用品消毒前后细菌污染调查

目的了解武汉市旅店业卫生间用品的卫生状况。方法随机采样监测了武汉市１０家旅店卫生间三大卫生用品（坐便器座垫、浴池、洗面盆）消毒前后细菌污染情况。结果武汉市旅店业卫生间三大卫生用品细菌污染较严重;消毒处理后,细菌总数均值虽有降低但仍较高,并可检出大肠菌群。结论旅店卫生间清洗消毒工作不力,亟待加强     

当归补血汤对细胞免疫调节作用及超微结构的影响

本实验采用环磷酰胺(CY)制造免疫功能低下小鼠的动物模型,研究当归补血汤组成药物的不同比例配伍,对机体免疫功能调节作用的影响。实验过程中将当归、黄芪分别配制成1∶1(A方)、1∶5(B方)、5∶1(C方)的不同比例中药方剂,对小鼠施以灌胃,并设对照组,以WBC总数、T淋巴细胞转化率及胸腺细胞超微结构变化作为检测指标。结果表明:三组不同比例配伍的方剂,对小鼠机体免疫功能均有调节作用。三组之间比较,提高WBC总数以A方效果最明显;三个方剂均有促进淋巴细胞转化的作用,但转化率三者之间无明显差异;保护胸腺细胞作用以B方效果最好。应用:当归补血汤对机体免疫功能有明显调节作用,但不同比例配伍,对机体免疫功能调节作用各有所侧重     

Cohort study of the total leukocyte count and periodontal disease among company employees

There have been few epidemiological studies of the relationship between the leukocyte count and dental disease. In the present study, therefore, we investigated the relationship between oral health indicated by the Community Periodontal Index of Treatment Needs (CPITN) and the total leukocyte count in the cohort study. The 1,035 subjects were male factory workers employed at a chemical factory in Osaka, Japan. Their oral conditions were recorded as the CPITN score. The relationship between the total leukocyte count and the oral condition of the subjects classified according to their smoking habits was investigated over a 5-year period. Among the current smokers, the total leukocyte count was highest each year for the group with CPITN level 4, followed by those with CPITN levels 3 and 2 in descending order, showing that the total leukocyte count was reduced as the periodontal diseases ameliorated. Among the nonsmokers, the total leukocyte count was high every year in the group with CPITN levels 4 or 3 compared to that of the group with CPITN level 2. The fluctuations of the total leukocyte count in current smokers and nonsmokers whose CPITN score increase or decreased in five years was investigated. There were no significant differences among the all groups.     

ITP患者脾动脉栓塞前后血小板的检测及其临床意义

应用自动血细胞计数仪检测ＩＴＰ患者脾动脉栓塞前后不同时期血小板的变化。结果栓塞后患者血小板计数（ＰＬＴ）和平均血小板体积（ＭＰＶ）的变化呈负相关,但ＰＬＴ恢复正常后,ＭＰＶ仍高于正常。提示脾动脉栓塞后血小板计数增高的机理不同于激素治疗。