ENDOSCOPIC MICROVASCULAR ARCHITECTURE OF THE PORTAL HYPERTENSIVE GASTRIC MUCOSA ON NARROW BAND IMAGING

Background: We evaluated the endoscopic microvascular architecture of the gastric mucosa in portal hypertension patients using the prototype of narrow band imaging (NBI). Material and Methods: The study included 103 Helicobacter pylori‐negative patients with chronic liver disease (22 without portal hypertension (group 1), 81 with portal hypertension (group 2)). Results: (i) Abnormality of collecting venules, reddening mucosa, red spots, a mosaic‐like pattern, and gastric antral vascular ectasia (GAVE) were observed on the gastric mucosa, and an obscure change in collecting venules (73% vs 14%; P < 0.001), reddening mucosa (49% vs 5%; P < 0.001), red spots (36% vs 5%; P < 0.01) and a mosaic‐like pattern (40% vs 5%; P < 0.01) were more frequently observed in group 2 than in group 1. (ii) On magnifying endoscopy with NBI, the mucosa with an obscure change in collecting venules was demonstrated as dilation of the capillaries surrounding the gastric pits in various degrees, and reddening mucosa was observed as extended and swollen gastric pits and various degrees of dilated and convoluted capillaries surrounding the gastric pits. Red spots were demonstrated as extended and swollen gastric pits, dilated and convoluted capillaries surrounding the gastric pits, and intramucosal hemorrhage around these capillaries. GAVE was recognized as partial and marked dilatation of the capillaries surrounding the gastric pits. Conclusion: Abnormality of collecting venules, swelling of gastric pits, dilatation of capillaries surrounding the gastric pits, intramucosal hemorrhage around capillaries, and partial and marked dilatation of the capillaries were observed on the gastric mucosa in portal hypertension patients.     

Quantification of the expression level of integrin receptor alpha(v)beta3 in cell lines and MR imaging with antibody-coated iron oxide particles.

Targeted imaging requires site-specific accumulation of a contrast agent (CA), and the properties of that agent must be selected according to the abundance of the target to obtain a signal above the detection limit of the instrument. However, numerical estimates of receptors per cell are rarely found in the literature. Integrin receptors would be particularly promising targets because of their accessibility from the blood stream and expression on activated neovascular endothelial cells. We systematically estimated the number of integrin receptors of cell lines and primary cells by flow cytometry analysis. Since integrin receptors are heterodimeric molecules, and alpha(v) forms complexes with various beta subunits, the numbers of alpha(v) and beta(3) subunits are therefore dissimilar. The observed values are 3 . 10(3)-1.4 . 10(4)/cell for alpha(v), and 5.3 . 10(2)-1.1 . 10(4)/cell for beta(3). Despite the low number of exposed receptors, we show that up to single-cell MR visualization can be achieved with the use of iron oxide beads complexed with antibodies as CAs.     

高脂血症患者血小板体积及有关参数的研究

本文报道242例高脂血症患者血小板(PLT)、平均血小板数(MPLT)及平均血小板体积(MPV)等参数的测定结果,其中高胆固醇并高甘油三酯患者,PLT、MPLT及巨大血小板比例(Macro-PLT)高于正常,MPV正常;而单纯高胆固醇或高甘油三酯患者,上述指标与正常人无显著性差异。提示高胆固醇并高甘油三酯患者,存在血小板生成动力学异常,其血小板的破坏增加,生成率加速、但处于一种高水平的动态平衡之中。这可能是高脂血症出现高凝状态的原因之一。同时表明此类患者使用抗血小板疗法具有一定的合理性和必要性。     

Smoothing the mosaic subclinical melanoderma by calcipotriol

BACKGROUND: The observation of photo-exposed skin under ultraviolet light reveals a mosaic pattern of varying intensity in epidermal melanization. Several patterns of mosaic subclinical melanoderma (MSM) have been described using a specially designed CCD camera and the ultraviolet light-enhanced visualization (ULEV) method. Vitamin D(3) and its analogues influence the biology of keratinocytes and melanocytes. OBJECTIVE: To assess the effect of calcipotriol on MSM. METHODS: This randomized split-face study was conducted in 27 men to compare the effect of once daily applications of 5% calcipotriol cream or a moisturizing cream on the heterogeneity of facial MSM. Computerized image analysis of video images was used at 1-month intervals before and during a 2-month treatment, as well as during a 3-month follow-up. RESULTS: At both sites, the average melanin content of the epidermis showed no significant change over time. However, the mottled appearance was smoothened at the calcipotriol site, whereas it was increased at the site receiving the moisturizer. CONCLUSION: The decreased heterogeneity in MSM after calcipotriol applications suggests a control of the epidermal melanocyte unit by the vitamin D(3) derivative.     

脊柱化脓性骨髓炎的诊断及治疗

目的探讨脊柱化脓性骨髓炎的诊断及治疗方法.方法1999年7月～2003年8月,收治以腰痛伴发热为主要临床表现的患者12例,男7例,女5例;年龄13～61岁,平均36.6岁.患者先有胸、腰背部疼痛,11例出现剧烈疼痛,伴有体温升高(超过39℃),局部无红肿,有深压痛及叩击痛,脊柱活动受限,但无脊髓及马尾神经压迫症状,二便正常.对患者行白细胞计数、血沉和MR等检查.12例患者中,10例血沉超过60 mm/1 h,1例血沉为28mm/1 h,1例血沉为50mm/1 h.2例患者白细胞计数超过1.0×109/L.4例患者经椎弓根穿刺活检,阳性率为75%.入院后即给予静脉滴注抗生素治疗至少6周,继而口服抗生素治疗6周,11例患者血沉于治疗后1个月下降50%以上,约91.7%的患者保守治疗有效.结果12例患者9例获得随访,随访时间6个月～4年,平均2年2个月.获得随访的9例患者中,7例无临床症状,1例有轻微背痛,1例有慢性严重背痛的患者,经保守治疗无效后,行手术治疗;7例行MR检查,2例显示局部椎体及椎间盘仍有破坏,T1加权像示低信号范围缩小,T2加权像示仍为高信号,但无腰背痛等临床症状.结论脊柱化脓性骨髓炎患者血沉较白细胞计数敏感;MR及病理检查对明确诊断有重要意义.采用静脉滴注抗生素及口服抗生素序贯疗法治疗1个月后血沉下降是保守治疗成功的标志,保守治疗无效者可手术治疗.     

血细胞彩色图像的微机自动计数方法探讨

目的　探讨血细胞自动计数的方法。方法　采用微机彩色图像处理技术对血液细胞图像进行图像分割和细胞计数。结果　与人工计数相平行 ,相关系数r =0 .96 1～ 0 .997,P >0 .0 5。结论　该方法可行且计数相对精确、迅速、客观     

螨类接种豚鼠气管引起的肺部病变

本文首次报导了螨类接种豚鼠气管所引起的肺部病变。螨类接种后的第6天发现肺部已形成典型的结节性病灶。结节呈圆锥形,淡黄色,直径1～5mm之间。镜下主要表现为细支气管和细支气管周围的病变。和早年报导的猴肺螨病肺部病变比较,有以下不同,肺实质内有明显的异物肉芽肿形成,炎症反应中嗜酸性细胞浸润不明显,而有更多的异物巨细胞形成;细支气管周围的小动脉受到损害:部分小动脉壁平滑肌明显增厚,以致腔隙变窄,部分小动脉腔内可见到虫体。根据我们实验研究证明,螨类接种豚鼠气管可引起肺部的典型病变.因而认为用豚鼠作为肺螨病的动物模型是有希望的。     

人体蠕形螨感染的皮肤组织病理学观察

目的:观察人体蠕形螨感染所致皮肤组织病理学改变。方法:收集7例面部皮肤标本经HE染色后在显微镜下观察。结果:在患者面部皮损处扩大的毛囊和增生的皮脂腺中检查出大量蠕形螨成虫、若虫和虫卵。人体毛囊蠕形螨多寄生于毛囊的根部或毛囊周围组织内,被寄生的毛囊均有不同程度的扩张,虫体周围常可见淋巴细胞为主的慢性炎症细胞浸润,毛根角质化较常见。人体皮脂蠕形螨寄生部位为皮脂腺体及导管部,可见腺细胞增生而失去正常结构,但较少有炎症细胞浸润,导管部有轻度扩张。结论:人体蠕形螨寄生与皮肤组织病理学改变有密切关系。     

股骨多段闭合骨折病人术前凝血功能的变化

目的 探讨股骨多段闭合骨折病人术前凝血功能的变化。方法 选择创伤股骨多段闭合骨折后当天入院的病人20例,年龄19-45岁,ASA Ⅰ级,为试验组(Ⅰ组);选择健康成年人15人,年龄21-39岁,作为对照组(Ⅱ组);Ⅱ组于清晨空腹采上肢静脉血标本,Ⅰ组病人入院后于骨折的第2天、第6天(手术当天清晨)空腹采上肢静脉血样本,检测血栓弹力图(TEG)指标[R时间、K时间、α角、血栓最大幅度(MA)、血栓硬度(G)]、D-二聚体浓度(D-Di)、血小板计数(PLC)及血小板聚集率(PAgR)的变化。结果 Ⅱ组TEG指标、D-Di、PLC及PAgR均在正常范围。与Ⅱ组比较,Ⅰ组骨折后第2天,K时间缩短(P<0.05),α角、MA、G及D-Di增高(P<0.01);骨折后第6天,R时间缩短(P<0.05),α角、MA、G、D-Di、PLC及PagR增高(P<0.01)。与骨折后第2天比较,Ⅰ组骨折后第6天MA、G、PLT及PAgR增高(P<0.05或0.01)。结论 病人创伤骨折后凝血功能24 h内增强,随时间的延长至术日呈高凝状态,应加强术中管理,并采取相应措施预防术中静脉血栓的发生。     

大学生蠕形螨感染的调查分析

目的调查广州医学院2001级大学生人体蠕形螨的感染情况,探讨蠕形螨感染与皮肤疾病、宿舍分布的关系。方法采用透明胶纸粘贴法对468名在校大学生蠕形螨感染进行调查,同时观察其面部皮肤状况,询问其所住宿舍状况并记录于调查表内。结果广州医学院2001级大学生468人,检出人体蠕形螨者85人,感染率18.16%。男学生感染率为18.37%(52/283人),女学生为17.84%(33/185人),两者无显著性差异(P>0.05)。从学生来源地看,城镇学生感染率为15.94%(51/320人),农村学生为22.97%(34/148人),两者无显著性差异(P>0.05)。感染者的感染度以每条透明胶纸1～3条虫为多,占87.06%(74/85人)。检获的螨大多为毛囊蠕形螨,占70.59%(60/85人),单一感染皮脂蠕形螨仅15.29%(13/85人),两者混合感染为14.12%(12/85人)。面部有痤疮、毛囊炎或毛囊扩张者蠕形螨感染率均高于面部皮肤正常、有痒感者(P<0.05);有痒感者蠕形螨感染率与面部皮肤正常者无显著性差异(P>0.05)。感染蠕形螨者具有显著的宿舍聚集性(P<0.01)。结论广州医学院2001级大学生人体蠕形螨感染较为普遍。感染大多为毛囊蠕形螨,少数为皮脂蠕形螨及两者混合感染,两种蠕形螨的感染度都属轻度。蠕形螨感染可能是痤疮、毛囊炎或毛囊扩张等皮肤疾病的致病因素之一。蠕形螨感染可以通过同