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81.
Francesco Tedesco Maria A. Villa Peter Densen Girolamo Sirchia 《Molecular immunology》1983,20(1):47-51
Structural and functional studies were performed on a dysfunctional C8 molecule present in the serum of two siblings and an unrelated individual. The C8 in these three sera exhibited a pattern of partial immunologic identity with C8 in normal serum but was devoid of functional activity. The C8 was immunoprecipitated from the three sera and from a control serum with an antihuman C8 antiserum and analyzed by SDS-PAGE using highly purified human C8 as a reference. A selective absence of a band of 62,000 mol. wt was observed in the immunoprecipitates from the sera containing dysfunctional C8. Experiments performed with the purified α-γ and γ subunits showed that the hemolytic activity of the C8 deficient sera could be reconstituted by the addition of the β chain but not the α-γ dimer. Binding of the dysfunctional C8 to C was excluded by the following observations: (1) EAC 1–7 treated with the C8 deficient sera and then washed could not be lysed after the addition of the β subunit and C9; and (2) the abnormal molecules did not interfere with the consumption of normal C8 by the soluble complex SC5b-7. 相似文献
82.
Shao Zezhi Li Yuping Krishnamoorthy Ramesh Chermak Todd Mitra Ashim K. 《Pharmaceutical research》1993,10(2):243-251
Various surfactants were investigated to compare their effects on insulin dissociation, -chymotryptic degradation, and rat enteral absorption. With a circular dichroism technique, sodium dodecyl sulfate (SDS) at a 5 mM concentration was found to completely dissociate procine-zinc insulin hexamers (0.5 mg/ml) into monomers. The catalytic activity of -chymotrypsin (0.5 µM) was also abolished by 5 mM SDS. When insulin was injected into the distal jejunum/ proximal ileum segment of the rat, 5 mM SDS greatly enhanced its pharmacological availability, from a negligible value to 2.8%. Being a cationic surfactant, hexadecyl trimethylammonium bromide (CTAB) also efficiently dissociated insulin hexamers at concentrations of 1–5 mM. However, extensive charge–charge interaction was observed below a CTAB concentration of 0.6 mM, leading to insulin precipitation at a molar CTAB:insulin ratio of 1:1 to 2:1. An -chymotryptic degradation study also revealed near-complete dissociation of insulin hexamers at 1 mM CTAB. Above 1 mM, however, CTAB acted as an enzyme inhibitor, most likely by means of charge repulsion. Enteral absorption studies showed a much lower pharmacological availability, only 0.29%. Nonionic surfactants such as Tween 80 and polyoxyethylene 9 lauryl ether were ineffective in dissociating insulin hexamers. Tween 80, at 5 mM, neither significantly altered the -chymotryptic degradation pattern nor enhanced the enteral absorption of insulin. The relative effectiveness of different species of bile salts on insulin hexamer dissociation appeared to be similar. Sodium glycocholate at a 30 mM concentration also significantly increased insulin pharmacological availability, to 2.3%. A morphological study did not reveal any significant alteration of the rat intestinal mucosal integrity after exposure to 5 mM SDS for 30 min. The results further emphasize the importance of the degree of insulin aggregation on its enteral transport. 相似文献
83.
目的 :探讨硫酸镁对缺血再灌注兔脑损伤的作用。方法 :15只兔随机均分至 :对照组、缺血组和缺血+硫酸镁处理组。阻断兔脑血管 6分钟 ,诱导全脑缺血。缺血再灌注 3天后 ,分别用HE染色和TUNEL染色 ,检测海马CA1 区神经元密度和凋亡神经元密度。结果 :缺血 +硫酸镁处理组海马CA1 区正常神经元密度为 140 .5 2±16 .2 3个 mm ,显著高于缺血组 (P <0 .0 1) ;缺血神经元密度为 2 4.18± 3 .16个 mm ,显著低于缺血组 (P <0 .0 1) ;凋亡神经元密度为 18.40± 8.0 8个 mm ,显著低于缺血组 (P <0 .0 1)。结论 :硫酸镁具有减轻兔缺血再灌注性全脑损伤的作用 ,其作用机制可能同抑制缺血后神经元凋亡有关 相似文献
84.
The selectivity of different external binding sites for quaternary ammonium ions in cloned potassium channels 总被引:1,自引:0,他引:1
W. Jarolimek K. V. Soman M. Alam A. M. Brown 《Pflügers Archiv : European journal of physiology》1995,430(5):672-681
Tetraethylammonium (TEA) is thought to be the most effective quaternary ammonium (QA) ion blocker at the external site of K+ channels, and small changes to the TEA ion reduce its potency. To examine the properties of the external QA receptor, we applied a variety of QA ions to excised patches from human embryonic kidney cells or Xenopus oocytes transfected with the delayed rectifying K+ channels Kv 2.1 and Kv 3.1. In outside-out patches of Kv 3.1, the relative potencies were TEA > tetrapropylammonium (TPA) > tetrabutylammonium (TBA). In contrast to Kv 3.1, the relative potencies in Kv 2.1 were TBA > TEA > TPA. In Kv 3.1 and Kv 2.1, external tetrapentylammonium (TPeA) blocked K+ currents in a fast, reversible and, in contrast to TEA, time-dependent manner. The external binding of TPeA appeared to be voltage independent, unlike the effects of TPeA applied to inside-out patches. External n-alkyl-triethylammonium compounds (C8, C10 chain length) had a lower affinity than TEA in Kv 3.1, but a higher affinity than TEA in Kv 2.1. In Kv 3.1, the decrease in QA affinity was large when one or two methyl groups were substituted for ethyl groups in TEA, but minor when propyl groups replaced ethyl groups. Changes in the free energy of binding could be correlated to changes in the free energy of hydration of TEA derivatives calculated by continuum methodology. These results reveal a substantial hydrophobic component of external QA ion binding to Kv 2.1, and to a lesser degree to Kv 3.1, in addition to the generally accepted electrostatic interactions. The chain length of hydrophobic TEA derivatives affects the affinity for the hydrophobic binding site, whereas the hydropathy of QA ions determines the electrostatic interaction energy. 相似文献
85.
Ba2+ differentially inhibits the Rb+ efflux promoting and the vasorelaxant effects of levcromakalim and minoxidil sulfate in rat isolated aorta 总被引:1,自引:0,他引:1
Ulrich Quast Yves Baumlin Cornelia Löffler 《Naunyn-Schmiedeberg's archives of pharmacology》1995,353(1):86-93
The K+ channel openers activate ATP-sensitive K+ channels (KATP) in vascular smooth muscle and induce relaxation. In this study, the relationship between these two effects was examined in rings of rat aorta using levcromakalim and minoxidil sulfate as the openers and Ba2+ as the K+ channel blocker; K+ channel opening was assessed by determining the rate constant of 86Rb+ efflux from the preparation.Ba2+ inhibited the 86Rb+ efflux stimulated by levcromakalim in a noncompetitive manner with an IC50 value of 29 M and a Hill-coefficient of 1.2. At concentrations > 300 M, Ba2+ increased the tension of rat aortic rings concentration-dependently. Levcromakalim relaxed contractions to Ba2+ (0.5 and 1 mM) with potencies similar to those determined against KCl (25 mM) or noradrenaline as spasmogens (EC50 values 15–40 nM). The vasorelaxant effect against Ba2+ was inhibited by the KATP channel blockers, glibenclamide and tedisamil, and abolished in depolarizing medium (55 mM KCl). At 3 mM Ba2+, levcromakalim was still able to transiently induce complete relaxation; however, within 1 h oscillations in tension developed, leading to a stable level of only 15% relaxation. A similar level of relaxation was achieved against 10 mM Ba2+ whereas the combination of 0.5 mM Ba2+ and 3 M tedisamil blocked the relaxant effect of levcromakalim completely. With minoxidil sulfate as the KATP channel opener the results of the 86Rb+ efflux and tension experiments were similar to those obtained with levcromakalim.It is concluded that Ba2+ is more potent in inhibiting the K+ channel opening than the vasorelaxant effects of the openers. On the basis of the 86Rb+ efflux experiments it is estimated that at least 97% of the channels opened by the activators can be blocked without major effects on vasorelaxation suggesting a dissociation between the two effects. However, if the block is pushed to extremes ( 99.95%) the vasorelaxant effect of the openers is also abolished suggesting a link between both effects. This paradoxon remains to be solved. 相似文献
86.
十二烷基硫酸钠在天然水体中的降解作用 总被引:1,自引:0,他引:1
本文研究了十二烷基硫酸钠在天然水体中的降解作用,结果表明十二烷基硫酸钠在天然水体中的降解主要依赖于微生物的降解。微生物最适降解条件为pH6.5~9.4和36℃左右。降解作用随温度升高(4~36.5℃)和微生物浓度增大而加快,用十二烷基硫酸钠预先驯化微... 相似文献
87.
为研究镁在脑缺血早期对神经元保护作用的机制,用电凝法建立大鼠大脑中动脉缺血模型,在造模前给大鼠注射20g/L硫酸镁,观察镁对脑组织中谷氨酸(Glu)及一氧化氮(NO)含量的影响。结果显示,高浓度镁可明显降低脑缺血皮质中Glu、NO含量,在缺血组Glu、NO的峰值浓度分别为9.87、44.97nmol/g,而在加镁组两者浓度分别为7.68、33.92nmol/g。提示:镁对脑缺血早期神经元的保护作用与其降低脑组织中Glu及NO含量有关。 相似文献
88.
季铵盐消毒剂中检出一株洋葱假单胞菌 总被引:4,自引:0,他引:4
目的 观察从复合季铵盐消毒剂中分离得到的洋葱假单胞菌对常用消毒剂的抗力。方法 以常用的氯和戊二醛消毒剂对其进行杀菌试验,并与对大肠杆菌和金黄色葡萄球菌相应的杀菌效果进行比较,以除菌后污染样液对大肠杆菌、金黄色葡萄球菌进行了杀菌试验和HBsAg 抗原性破坏试验,对芽胞和白色念珠菌进行抑菌试验。结果 该洋葱假单胞菌对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近,污染样液除菌后1 000 倍稀释对大肠杆菌和金黄色葡萄球菌仍有较好的杀菌效果,20 倍稀释可完全破坏HBsAg 抗原性,和抑制枯草杆菌黑色变种芽胞的生长,1 600 倍稀释可抑制白色念珠菌生长,而原液则不能抑制分离菌株的生长。结论 洋葱假单胞菌可在高浓度(1 .6% )季铵盐消毒剂中大量生长,其对该类消毒剂抗力明显高于大肠杆菌、金黄色葡萄球菌、白色念珠菌、枯草杆菌黑色变种芽胞和HBsAg,其对氯和戊二醛的抗力与大肠杆菌和金黄色葡萄球菌接近 相似文献
89.
硫酸奈替米星注射液与硫酸丁胺卡那注射液临床疗效及安全性的比较 总被引:5,自引:0,他引:5
为评价硫酸奈替米星的临床疗效及安全性,在170例急性细菌感染性病人中作多中心临床研究。结果显示:硫酸奈替米星的有效率(95.3%)显著高于对照药硫酸丁胺卡那的有效率(84.1%),试验组病人咳痰和腰痛症状的平均下降显著高于对照组;硫酸奈替米星治疗后细菌清除率达97.2%;2组病人不良反应发生率均为4.8%,对照组中有2例(3.2%)出现耳鸣、1例(1.6%)听力减退,但试验组病人用药后未发现听力异常。结论:硫酸奈替米星临床疗效优于硫酸丁胺卡那且较少耳毒性。 相似文献
90.
Several oxazolidinones having amine moiety were prepared to form a quaternary ammonium salt with cephalosporin nucleus, and antibacterial activity of the quaternary ammonium cephalosporin derivatives bearing oxazolidinone moiety were examined particularly with expectation of dual activity. However, the cephalosporin-oxazolidinone compounds revealed rather weaker antibacterial activity in vitro than their parent oxazolidinone and cephalosporin without showing any characteristic activity as expected. 相似文献