芦荟对银屑病患者外周血单核细胞和角质形成细胞增殖和凋亡的研究

目的探讨芦荟对银屑病患者外周血单一核细胞(PBMC)和角质形成细胞增殖和凋亡影响.方法选择寻常性银屑病患者PBMC,共20例,正常人16例,及角质形成细胞株Colo-16细胞.采用MTT方法检测细胞增殖,ELISA方法检测细胞凋亡.结果芦荟对正常人和银屑病患者PBMC增殖均有抑制作用(P＜0.01),无促进细胞凋亡作用,对角质形成细胞株Colo-16细胞有明显的抑制增殖和促进凋亡作用(P＜0.01).结论芦荟抑制银屑病患者PBMC的增殖和诱导角质形成细胞凋亡可能是芦荟治疗银屑病的重要药理作用之一.     

芦荟制品祛黄褐斑作用的人体试食观察研究

[目的 ]了解芦荟制品祛黄褐斑的保健作用。 [方法 ] 3 0例黄褐斑志愿者每人每日服用以芦荟为主要原料的软胶囊 2次 ,每次 1粒 ,每粒 1g ,连续 3 0d。比较受试者黄褐斑的颜色和面积。 [结果 ]受试者黄褐斑面积平均下降3 1 17± 2 6 5 9mm2 ,颜色明显变浅 ,色卡平均降低 0 5 9± 0 5 0度 (P <0 0 1) ,总有效率达 80 0 0 % ;且受试者血清SOD的活性较试食前有显著提高。试食前后血常规、尿常规及血生化指标无异常。 [结论 ]软胶囊具有明显的祛黄褐斑作用 ,且对试食者身体健康无明显影响     

Aloe vera: A review of toxicity and adverse clinical effects

The Aloe plant is employed as a dietary supplement in a variety of foods and as an ingredient in cosmetic products. The widespread human exposure and its potential toxic and carcinogenic activities raise safety concerns. Chemical analysis reveals that the Aloe plant contains various polysaccharides and phenolic chemicals, notably anthraquinones. Ingestion of Aloe preparations is associated with diarrhea, hypokalemia, pseudomelanosis coli, kidney failure, as well as phototoxicity and hypersensitive reactions. Recently, Aloe vera whole leaf extract showed clear evidence of carcinogenic activity in rats, and was classified by the International Agency for Research on Cancer as a possible human carcinogen (Group 2B). This review presents updated information on the toxicological effects, including the cytotoxicity, genotoxicity, carcinogenicity, and adverse clinical effects of Aloe vera whole leaf extract, gel, and latex.     

芦荟多糖对体外培养人表皮细胞分泌细胞因子及一氧化氮的影响

目的研究芦荟多糖对体外培养人表皮细胞分泌细胞因子及一氧化氮(NO)的影响。方法测定经25、50、100、200和400mg/L,不同浓度芦荟多糖作用后的人表皮细胞培养上清液中转化生长因子-α(TGF-α)、TGF-β、白细胞介素-1β(IL-1β)、IL-6、IL-8、肿瘤坏死因子(TNF)及NO的水平;对照组则用等体积的细胞培养液处理。结果与对照组比较,经芦荟多糖作用后,培养液中TGF-α、TGF-β1、IL-1β、IL-6、IL-8和TNF水平呈不同程度升高,其差异有显著性(P<0.05或P<0.01);且随着芦荟多糖作用浓度的增加,其效应与剂量明显相关(P<0.01);而NO水平与对照组比较呈显著性下降(P<0.01),量-效关系明显(P<0.01)。结论芦荟多糖促进人表皮细胞分泌TGF-α、TGF-β1、IL-1β、IL-6、IL-8及TNF,而对NO释放则具有抑制作用。     

芦荟临床外用研究进展

对芦荟外用功能的临床应用研究进展进行了综述。芦荟具有医疗、保健、美容、食用、观赏等多种功能,近年来芦荟外用品的开发研究,倍受青睐,应用范围日趋扩大,成为全世界关注的药食多用植物。     

辨证分型治疗甲状腺功能亢进症35例临床观察

[目的]观复辨证分型治疗甲状腺功能亢进症疗效。[方法]使用前瞻性设计方法,对30例门诊患者辨证治疗。肝郁痰火,海藻玉壶汤(海藻、昆布各15g,海带、法半夏各20g,陈皮10g,青皮8g,连翘、象贝、当归各15g,川芎8g,独活10g,甘草5g)。阴虚火旺,清肝芦荟丸合玉女煎(夏枯草25g,黄芩20g,黄连15g,生石膏先煎30g,知母、地骨皮、昆布各15g,海藻20g,白芍、生地、玉竹、麦冬各15g,牡蛎先煎30g,芦荟1.5g);胃腑热甚,津液耗伤,大便秘结加大黄后下30g,芒硝12g。气阴两虚,黄芪汤(黄芪、党参各20g,天冬15g,生地20g,白芍、花粉、茯神各15g,五味子10g,甘草5g);心烦善怒、性情急躁加夏枯草、龙胆草、黄芩各15g,栀子20g;心悸、口舌生疮加黄连、枣仁、远志各10g;大便稀溏加白术、山药各15g,神曲10g,谷芽15g。连续治疗30d为1疗程。1剂/d,水煎350m L。病情稳定后,配制蜜丸,9g/丸,1丸/d,早晚温水服用,巩固疗效,防止复发。观测临床症状、血清总三碘甲腺原氨酸(TT3)、血清总甲状腺素(TT4)、血清游离甲状腺素(FT4)、游离三碘甲腺原氨酸(FT3)、促甲状腺激素(TSH)、不良反应。连续治疗3疗程,判定疗效。[结果]临床治愈8例,基本治愈7例,显效6例,好转9例,无效5例,总有效率为85.71%(30/35)[结论]辨证分型治疗甲状腺功能亢进症疗效显著,无严重不良反应,值得推广。     

Reduction of 1,2-Dimethylhydrazine-induced Colorectal Proliferative Lesions in Mice by Aloe arborescens var. natalensis (Kidachi Aloe)

We examined the modifying effects of freeze-dried whole-leaf Aloe arborescens Miller var. natalensis Berger (Japanese name, Kidachi aloe; designated as ‘ALOE’) on 1,2-dimethylhydrazine (DMH)-induced colorectal tumorigenesis in mice. Female ICR mice (7-weeks old) were given a basal diet or a diet containing 1, 0.5 or 0.1% ALOE for 32 weeks. One week later, all mice were injected i.p. with DMH (20?mg/kg, once weekly for 10 weeks) or vehicle (1?mM?EDTA solution, pH 6.5). At 32 weeks, animals were killed by exsanguination, and the colorectums were processed for histological examination. The administration of ALOE (1, 0.5 or 0.1% in diet) did not induce diarrhea or reduction of body weight. In mice given DMH and 1% ALOE (Group 2), the incidence and multiplicity of colorectal adenomatous hyperplasias were significantly decreased as compared with mice given DMH alone (Group 1) (both p < 0.05), whereas the incidence and multiplicity of tumors (adenoma and adenocarcinoma) in Group 2 tended to be lower than those in Group 1. In addition, the incidence and multiplicity of the colorectal proliferative lesions (the total of adenomatous hyperplasias, adenomas and adenocarcinomas in mouse colorectum) in Group 2 were significantly decreased as compared with Group 1 (both p < 0.01). No colorectal proliferative lesions were found in animals that did not receive DMH. These results indicated that ALOE reduces the incidence and multiplicity of DMH-induced colorectal proliferative lesions, especially adenomatous hyperplasia, in mice.     

The efficacy of aloe vera gel in the treatment of oral lichen planus: a randomized controlled trial

BACKGROUND: Oral lichen planus (OLP) is a chronic inflammatory disease that can be painful especially in the atrophic and erosive forms. Several therapies have been tried, with varying results. There is one case report in which aloe vera (AV) was used successfully in the treatment of lichen planus. OBJECTIVES: To compare the efficacy of AV and placebo in the topical management of OLP. METHODS: A randomized, double-blind, placebo-controlled trial was designed. Fifty-four patients were randomized into two groups to receive AV gel or placebo for 8 weeks. RESULTS: Fifty-four consecutive patients (34 women and 20 men) participated in the study. We found erosive and ulcerative lesions in 83% and 17%, respectively. The most common site of OLP was the lower lip. Twenty-two of 27 patients treated with AV (81%) had a good response after 8 weeks of treatment, while one of 27 placebo-treated patients (4%) had a similar response (P<0.001). Furthermore, two patients treated with AV (7%) had a complete clinical remission. Burning pain completely disappeared in nine patients treated with AV (33%) and in one treated with placebo (4%) (P=0.005). Symptomatology improved by at least 50% (good response) in 17 patients treated with AV (63%) and in two treated with placebo (7%) (P<0.001). No serious side-effects were found in both groups. CONCLUSIONS: AV gel is statistically significantly more effective than placebo in inducing clinical and symptomatological improvement of OLP. Therefore, AV gel can be considered a safe alternative treatment for patients with OLP.