冠状动脉内支架术后即刻明胶酶变化的研究

目的：探讨在急性心肌梗塞患者冠状动脉支架术（PTCA）明胶酶的变化；方法：15例确诊为单支血管病变的急性心肌梗塞患者，在PTCA术前及术后5分钟内冠状动脉窦内取抗凝血2ml，利用Gelatin—PAGE法测冠状动脉内明胶酶的活性表达；结果：在术后明胶酶A比术前高（P〈0．05），明胶酶B与术前无明显变化（P〉0．05）。结论：明胶酶可能参与支架术后再狭窄过程。     

Metabolic changes by acute insulin deficiency in rabbits injected with anti-insulin serum

Summary Acute insulin deficiency was produced in rabbits treated with anti-insulin guinea pig serum. The changes in the levels of blood sugar, free fatty acids, aceto-acetate, SGOT, SGPT, liver glutamicoxaloacetic and glutamic-pyruvic transaminases, and liver lactate dehydrogenase were examined. A significant increase of the blood glucose level and the free fatty acids as well as glycosuria was observed. The other examined parameters remained unchanged. The results confirm the statement that acute insulin deficiency causes a prompt impairment of glucose and fat metabolism.

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Zusammenfassung Ein akuter Insulin-Mangelzustand wurde in mit anti-Insulinserum vom Meerschweinchen behandelten Kaninchen hervorgerufen. Es wurden die Veraenderungen des Blutzuckerspiegels, der freien Fettsaeuren, des Azetoazetats, der SGOT, SGPT, der Glutaminsaeure-Oxalessigsaeure-Transaminase und der Glutaminsaeure-Pyruvat-Transaminase, sowie der LDH in der Leber untersucht. Es wurde eine signifikante Steigerung des Blutzuckers, der freien Fettsaeuren und auch des Harnzuckers festgestellt. Die anderen untersuchten Parameter blieben unveraendert. Die Resultate bestaetigen den Tatbestand, dass der akute Insulinmangelzustand eine sofortige Stoerung des Kohlenhydrat- sowie des Fettstoffwechsels ausloest.

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Resumen Una carencia insulínica aguda ha sido provocada en los conejos tratados con suero de cobayo anti-insulina. Han sido estudiadas las variaciones en los niveles de la glucemia, de los ácidos grasos libres, del acetoacetato, de la SGOT, de la SGPT, de las transaminasas glutámico-oxaloacética y glutámico-pirúvica hepáticas y de la láctico-dehidrogenasa hepática. Ha sido puesto de manifiesto un aumento significativo de la glucemia y de los ácidos grasos libres, y también de la glucosuria. Los otros parámetros examinados han quedado invariados. Los resultados ratifican el hecho de que la carencia aguda de insulina provoca una alteración inmediata en el metabolismo de los glúcidos y de los lípidos.

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Resume On a provoqué une carence en insuline aïgue chez des lapins traités au sérum de cobaye antiinsuline. On a étudié les variations de taux de glycémie, des acides gras libres, d'acéto-acétate, de SGOT, de SGPT, des transaminases glutamique oxalacétique et glutamico-pyruvique hépatiques ainsi que de la déshydrogénase lactique hépatique. On a observé une augmentation significative de la glycémie et des acides gras libres, et même de la glycosurie. Les autres paramètres étudiés sont restés inchangés. Les résultats confirment le fait que la carence aïgue en insuline provoque une perturbation immédiate du métabolisme des glucides et des lipides.

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Riassunto Una carenza insulinica acuta è stata provocata nei conigli trattati con siero di cavia anti-insulina. Sono state studiate le variazioni del livello della glicemia, degli acidi grassi liberi, dell'acetoacetato, della SGOT, della SGPT, delle transaminasi glutammico-ossalacetica e glutammico-piruvica epatiche e della lattico deidrogenasi epatica. E' stato rilevato un aumento significativo della glicemia e degli acidi grassi liberi, ed anche della glicosuria. Gli altri parametri esaminati sono rimasti invariati. I risultati confermano il fatto che la carenza acuta di insulina provoca un disturbo immediato del metabolismo glucidico e lipidico.

     

Plasma proteome of severe acute respiratory syndrome analyzed by two-dimensional gel electrophoresis and mass spectrometry

We have investigated the plasma proteome by using 2D gel electrophoresis and MS from patients with severe acute respiratory syndrome (SARS). A complete proteomic analysis was performed on four patients with SARS in different time courses, and a total of 38 differential spots were selected for protein identification. Most of the proteins identified are acute phase proteins, and their presence represents the consequence of serial cascades initiated by SARS-coronavirus infection. There are several proteins that have never been identified in plasma before using 2D gel electrophoresis, among which peroxiredoxin II was chosen for further study by analyzing additional 20 plasma samples from patients with probable and suspected SARS and patients with fever, respectively. The results showed that the level of plasma peroxiredoxin II in patients with SARS is significantly high and could be secreted by T cells. Taken together, our findings indicate that active innate immune responses, along with the oxidation-associated injuries, may play a major role in the pathogenesis of SARS.     

Initial Blood Pressure Response to Captopril in Patients with Acute Myocardial Infarction, Related Factors and Mechanism

在82例急性心肌梗塞（AMI）的病人中探测使用卡托普利的初始血压反应的因素及机制。结果显示AMI病人对卡托普利的初始血压反应与心肌梗塞的部位、基础血压水平、心功能状态、RAS的活性及使用卡托普利的时间有关，RAS对卡托普利的反应可能是初始血压反应的主要机制。     

部分去腐治疗乳牙急性龋的Meta分析

目的比较部分和完全去腐治疗乳牙急性龋的效果。方法计算机检索The Cochrane Library、Pub Med、EMbase、Wan Fang Data、CBM、CNKI数据库,查找相关随机对照实验(RCT),检索时限为1978年—2015年8月。由2位评价员独立筛选文献、提取资料和评价研究质量后,采用Rev Man 5.3软件进行Meta分析。结果最终纳入6个RCT,共计562例患者,758个患牙。Meta分析结果显示:部分去腐的牙髓暴露可能性低于完全去腐,差异有统计学意义[RR=0.13,95%CI(0.05,0.37)];在术后牙髓病变(RR=0.92,95%CI(0.34,2.50))和修复失败(RR=1.07,95%CI(0.67,1.70))方面,两者差异没有统计学意义。结论部分去腐法治疗乳牙急性龋的效果是肯定的,值得在临床工作中推广使用。由于纳入研究设计存在一些缺陷,还需要今后开展更多高质量,大样本的随机对照实验来验证此结论。     

Thrombin: Structure, Biochemistry, Measurement, and Status in Clinical Medicine

Thrombin remains a molecule of great interest to scientists and clinicians alike because of its important role in hemostasis, thrombosis, inflammation and vascular remodeling. Yet one of the great challenges has been the inhibition of thrombin generation to a degree that minimizes intravascular thrombosis while preserving physiologic hemostasis. It has become increasingly clear that high levels of anticoagulation with either direct or indirect thrombin antagonists are not beneficial and, in fact, are quite detrimental. Despite the overwhelming shift of interest toward the platelet in clinical trials of acute coronary syndromes, much can be gained through further investigation of coagulation processes responsible for thrombin generation and activity.     

Intracoronary stenting of a coronary occlusion resulting from an aortic dissection

Acute myocardial infarction secondary to aortic dissection may occur due to compression of the coronary arteries by a hematoma or extension of the dissection into the coronary arterial wall. We report a patient with this condition who was successfully treated by Palmaz-Schatz stenting, providing an alternative to thrombolysis or angioplasty. © 1995 Wiley-Liss, Inc.