新生儿重组酵母乙肝疫苗接种免疫的研究

目的：探讨重组酵母乙肝疫苗新生儿接种后抗体应答持续时间，强化复种的必要性。方法：常规对新生儿0d(出生后当天)、1月、6月接种重组酵母乙肝疫苗后检测抗体应答情况，再随机分两组，一组为强化组，一组为常规组，并随访其5年的抗体应答情况。结果：新生儿常规接种法，于12个月后有一明显抗体应答下降期(0d、1月、6月、12月)，强化组乙肝表面抗体形成率高，持续时间均高于常规接种法。结论：新生儿乙肝疫苗0d、1月、6月、12月接种法有较好的远期保护效果，对现有的0d、1月、6月新生儿乙肝疫苗常规接种法，有强化接种的必要。     

秦巴山区野生与栽培猪苓菌核主要成分的测定

采用原子吸收分光光度法、高效液相色谱等方法对秦巴山区野生与栽培猪苓菌核的氨基酸、水分、灰分、粗蛋白、粗脂肪、粗纤维、矿质元素、猪苓多糖等主要成分及含量进行测定.结果表明,野生和栽培猪苓在所测定的17种氨基酸中,7种必需氨基酸占氨基酸总量的质量分数分别为33.8%,23.6%.每100g野生与栽培猪苓菌核中水分质量分别为13.22g,11.94g;灰分质量分别为2.86g,2.61g;粗蛋白质量分别为6.87g,4.91g;粗纤维质量分别为27.62g,26.02g;粗脂肪质量分别为1.87g,1.71g;每100g野生和栽培猪苓质量分别为1.87g,1.71g;猪苓多糖质量分别为0.51g,0.40g.     

Implantation of Active Can Implantable Defibrillators in the right Pectoral Region

Routinely the active can ICD is placed in the left side pectoral position, which theoretically gives optimal conditions for a low defibrillation threshold. Some patients, bowever, demand a right pectoral position, which possibly could result in a bigger defibrillation threshold. A right pectoral position was used in 3 of 85 active can ICDs implanted in our institution from 1994. the DFT was 12 J in two and 18 f in one patient. Thus, right pectoral implantation is feasible and offers an alternative approach in selected patients.     

Multiple Implants for First Molar Prosthodontics

Problems that can occur when single implants are utilized to restore first molar teeth include the frequent loosening of screws, as well as screws and/or implant breakage. These may result from torquing and rotational movements of the prosthesis during masticatory and parafunctional mandibular movements. When sufficient bone and mesio-distal restorative space is present, the placement of two implants should be considered.     

A protease inhibitor attenuates gastric erosions and microcirculatory disturbance in the early period after thermal injury in rats

The effects of camostat mesilate, a synthetic serine protease inhibitor on gastric microcirculation and active oxygen species generated by leucocytes from the gastric and jugular veins in the early period after thermal injury were assessed. Male Wistar rats were anaesthetized and a 30% full skin-thickness dorsal burn was inflicted. Camostat mesilate (100 mg/kg) was dissolved in distilled water and administered orally to rats 40 min before thermal injury (the camostat group). The control animals (the vehicle group) were administered distilled water orally. Rolling leucocytes as well as Monastral blue B deposits in venules were observed using in vivo microscopy. Active oxygen species were measured by chemiluminescence. Camostat mesilate decreased the total length of gastric erosion, venular deposits of Monastral blue B, and rolling of leucocytes in venules, and relatively increased luminol-dependent chemiluminescence activity generated by zymosan-stimulated leucocytes 15 min after thermal injury. These results suggest that serine proteases are involved in the formation of gastric erosions and gastric microcirculatory disturbance in the early period after thermal injury.     

活血祛风法治疗慢性活动性乙型肝炎临床观察

用活血祛风法治疗反复发作的慢性活动性乙型肝炎７０例，总有效为９５．７％，对照组６５例健脾清热利湿法治疗，总有效率为７８。５％（Ｐ＜０．０５）．治疗组抗ＬＳＰ，ＬＭＡ，抗核抗体，类风湿因子阴转率，ＨＢｅＡｇ，抗ＨＢｃＩｇＭ，ＨＢＶ－ＤＮＡ阴转率，抗ＨＢｅ阳转率均优于对照组（Ｐ＜０．００１）。     

新型阳离子(苯乙烯—丙烯酰胺)共聚物的研究 Ⅱ.阳离子(苯乙烯——丙烯酰胺)共聚物与染料的相互作用

以P(St-Am·MG)对染料活性艳红(X-3B)溶液进行絮凝试验,考察了高聚物剂量及外加盐对絮凝效果的影响,并与无疏水基团的阳离子聚丙烯酰胺PAm·MG的絮凝能力进行了比较。发现絮凝体系无外加盐时,高聚物与染料的作用主要是通过静电力;有外加盐时,高聚物的电荷受到屏蔽,高分子链卷曲,PAm·MG的絮凝效果受到较大影响。而P(St-Am·MG)与X-3B之间存在疏水相互作用,受影响较小,此时P(St-Am·MG)的絮凝性能优于PAm·MG。用平衡渗析法研究了不同温度时甲醇和脲存在下P(St-Am·MG)与X-3B相互作用的热力学。证明了它们之间确实存在疏水相互作用。     

Tyrosine hydroxylase and serotonin containing cells in embryonic rat rhombencephalon: a whole-mount immunocytochemical study

Rhombencephala from rat embryos were processed as whole-mounts for immunocytochemical detection of monoaminergic cell populations, using antibodies to tyrosine hydroxylase (TH) and serotonin (5-HT). Specific advantages of the whole-mount technique over the classical serial-section method were that even isolated immunoreactive (IR) cells could be detected easily, and three-dimensional relationships could be ascertained without the need for serial reconstruction. Embryos between embryonic days (E) 12 and 16 (the day following nocturnal mating being considered as E1) were used in this study. Both TH and 5-HT immunoreactivities were already detectable at E12, even in the smallest embryos (crown-rump length: 6 mm), but there was a striking difference in the number and regional distribution of these two types of IR cells. TH was expressed in several cell groups located in the rostral rhombencephalon (the presumed anlage of the A4-7 complex) as well as in the caudal rhombencephalon (the presumed anlagen of groups A1-2 and C1-3), whereas 5-HT was expressed in very few cells located near the rostral border of the rhombencephalon (presumed anlage of the B4-9 complex). Although the three-dimensional distribution of the TH-IR cell groups underwent some modifications during the period studied, its general pattern remained relatively stable after E12. This contrasted with the sequential appearance of the 5-HT-IR cell groups and their spatial transformations during this period. Using the rhombencephalic isthmus as a landmark, we found that conspicuous 5-HT-IR fibre bundles penetrated into the mesencephalon from E13 onwards, but that the 5-HT IR cell bodies were exclusively located caudal to the borderline between the mesencephalon and the rhombencephalon (the rhombencephalic isthmus). We therefore suggest the term "rostral rhombencephalic raphe nuclei" for the rostral 5-HT cell groups instead of "mesencephalic raphe nuclei," which is a misnomer. Close spatial association between TH and 5-HT-IR elements was observed mainly in the caudal rhombencephalon, where 5-HT-IR fibres coursed through an area containing numerous TH-IR cell bodies (the presumed anlagen of groups A1-2 and C1-3).     

活动性肺结核患者血清Fad D9重组蛋白、sCD14-ST及单核细胞P糖蛋白水平及其临床意义

目的探讨活动性肺结核(APTB)患者血清Fad D9重组蛋白、可溶性白细胞分化抗原14亚型(sCD14-ST)、单核细胞P糖蛋白(Pgp)表达及其临床意义。 方法选取96例APTB患者(APTB组),按照肺部病灶有无空洞和病灶肺叶数分亚组。同期选择体检健康且经γ干扰素释放试验检测阴性者(健康对照组,HC组)及阳性者(结核潜伏感染组,LTBI组)各48例。比较各组血清Fad D9重组蛋白、sCD14-ST、Pgp水平。评估Fad D9重组蛋白、sCD14-ST、Pgp诊断APTB和鉴别APTB、LTBI的价值。 结果Fad D9重组蛋白、sCD14-ST、Pgp水平APTB组＞LTBI组＞HC组,且有空洞者高于无空洞者,病灶肺叶≥3个者高于＜3个者(P＜0.05)。Fad D9重组蛋白、sCD14-ST、Pgp对APTB诊断和APTB、LTBI鉴别有良好效能(P＜0.05)。 结论APTB患者血清Fad D9重组蛋白、sCD14-ST及Pgp明显升高,且与肺部病灶的严重程度有关。     

Transfer factor (TF) treatment of patients with HBs-Ag-positive chronic active hepatitis

Summary It is a clinically and experimentally well supported working hypothesis that infection with hepatitis B virus may result in chronic active hepatitis in patients with suspected immune deficiencies. On this basis, a pilot study was performed in order to evaluate the effect of specific transfer factor (TF) in the treatment of HB_s-Ag-positive chronic active hepatitis. From the leukocytes of 500 ml venous blood each of 40 volunteers that had completely recovered from acute virus hepatitis B within the last 6 months, a unique TF pool (40 units of TF) was prepared according to the method of Lawrence. Preexaminations indicated that this preparation was able to enhance cellular immune reactions in vitro. Thirteen patients with HB_s-antigenemia and chronic active hepatitis (i.e., two liver biopsies within the last 6 or more months with the histological criteria of chronic aggressive hepatitis according to de Groote, elevated serum levels of bilirubin, alkaline phosphatase, transaminase activities, and/or -globulines) were randomized: Seven received s.c. injections of two units of TF each on days 1 and 15, the other six saline. Conversion of skin reactions to some ubiquitous antigens occurred in the TF group, but no significant and constant drop of HB_s-Ag serum titers was observed. Although some of the biochemical parameters seemed to ameliorate in the TF group, the differences versus the control group did not prove to be significant within the limited number of patients under observation. The in vitro reactivity of patients' lymphocytes to HB_s-Ag, tested by means of the³H-thymidine uptake, was never found enhanced after TF application. In the used doses, specific TF was not effective in the treatment of HB_s-Ag-positive chronic active hepatitis; unfavorable side-effects were not observed.