Network pharmacological analysis on the active ingredients of Yigan Powder in treating Alzheimer' s disease with depressive disorder

Objective: To study the potential therapeutic effect of Yigan Powder on Alzheimer's disease (AD) comorbid Depressive Disorder with Network Pharmacology. Methods: The active ingredients of Yigan Powder were screened from Traditional Chinese Medicine Systems Pharmacology database and analysis platform (TCMSP) by ADME parameters, targets are predicted by Swiss Target Prediction database, disease targets are downloaded from GeneCards, OMIM and PharGKB database. Using R to run GO terms enrichment and KEGG pathway enrichment analysis. Protein interaction data is downloaded from the String database. Cytoscape software is used to build network. AutoDock Vina and PyMOL software are used for molecular docking. Results: There are 125 active ingredients in Yigan Powder with 953 predicted targets, 85 of predicted targets are related to Alzheimer's Disease comorbid Depressive Disorder, shows highly enriched signaling pathways and biological processes. PPI network shows APP, MAPK1 and STAT3 may be important potential treatment of Alzheimer's Disease comorbid Depressive Disorder. The results of AutoDock Vina docking showed that the active ingredients of Yigan Powder had good binding activity with important receptors. Conclusion: Yigan Powder shows effect on neurotransmitter metabolism, synaptic transmission, neuroinflammation and other aspects in the treatment of Alzheimer's Disease comorbid Depressive Disorder.     

乳香、没药挥发油促九分散方中生物碱类成分的HaCaT细胞摄取及其机制研究

目的研究九分散中乳香、没药挥发油促进角质形成细胞HaCaT摄取马钱子碱、士的宁及盐酸麻黄碱的作用及其机制。方法采用CCK-8法检测乳香、没药挥发油单用/药对与九分散方中马钱子碱、士的宁、盐酸麻黄碱配伍对HaCaT细胞存活率的影响;采用液相色谱串联质谱(LC-MS/MS)定量分析法结合BCA蛋白试剂盒测定在乳香、没药挥发油作用下HaCaT细胞对3种生物碱(马钱子碱、士的宁及盐酸麻黄碱)细胞摄取的影响;以DiBAC4(3)为荧光探针,利用流式细胞仪测定乳香、没药挥发油对HaCaT细胞膜电位的影响。结果不同乳香、没药挥发油均对3种生物碱有一定促透作用,且对脂溶性成分马钱子碱、士的宁的促透效果较水溶性成分盐酸麻黄碱好。乳香、没药挥发油分别作用于DiBAC4(3)标记的HaCaT细胞后,可降低HaCaT细胞膜电位,随着乳香、没药挥发油浓度增加,细胞膜电位荧光强度逐渐增强,具有浓度相关性,表现出类似氮酮的作用方式。结论九分散中乳香、没药挥发油均可促进HaCaT细胞摄取3种生物碱,促进机制可能通过影响皮肤表面负电荷而改变皮肤活性表皮屏障作用,从而有利于药物透过皮肤活性表皮层,其具体机制有待进一步探明。     

银翘散君药之辨

银翘散为《温病条辨》代表方剂之一,该方具有辛凉透表,清热解毒之功效,主治温病初起,风温之邪侵袭肌表,组方配伍严谨精妙,也具有很高的学术价值和临床意义。分析条文解读吴鞠通的辨治思路,从配伍、治法多角度阐述银翘散应以辛凉解表药为君药,以解表透邪为立法,对指导临床辨证和选方用药有重要意义,以此能更好继承和发扬古代名医吴鞠通的思想精神。     

复方聚乙二醇电解质散在老年肠道准备的临床观察

目的 比较甘露醇、蓖麻油、复方聚乙二醇电解质散在老年科肠道镜检肠道准备的效果、耐受性及不良反应发生率.方法 选取门诊及同期住院进行肠镜检查的93例老年病人(分为甘露醇组31例,蓖麻油组32例,复方聚乙二醇电解质散组30例),对肠道准备过程中清洁肠道的效果、病人的耐受性和胃肠道反应情况进行比较.结果 甘露醇组、蓖麻油组、复方聚乙二醇电解质散组发生肠胃肠道反应者分别为7例、12例和3例,复方聚乙二醇电解质散组与前两组比较,p＜0.05;不能耐受者甘露醇组、蓖麻油组和复方聚乙二醇电解质散组分别为7例、9例和1例,复方聚乙二醇电解质散组与前两组比较,p＜0.05;肠道清洁度Ⅰ Ⅱ级者甘露醇组、蓖麻油组和复方聚乙二醇电解质散组分别为12例、11例和28例,复方聚乙二醇电解质散组明显优于前2组,p＜0.05.结论 复方聚乙二醇电解质散有明显的泻下作用,病人耐受性好,胃肠道反应少而轻,清洁肠道效果明显优于甘露醇和蓖麻油.     

玉屏风颗粒联合脾氨肽口服冻干粉在咳嗽变异性哮喘中的疗效观察

目的研究玉屏风颗粒联合脾氨肽口服冻干粉在咳嗽变异性哮喘中的临床疗效。方法收集CVA患儿99例,采用随机数字表法分成A、B、C组,每组33例,A组:玉屏风组;B组:脾氨肽组;C组:玉屏风联合脾氨肽组。每组均加用正规抗哮喘药物,总疗程3个月,观察3组的疗效情况,随访18个月。结果 3组疗效对比,差异无统计学意义(P>0.05);复发情况显示C组有较低的复发率;三组患儿均无明显不良反应。结论玉屏风颗粒联合脾氨肽口服冻干粉在中医辨证为肺气亏虚型咳嗽变异性哮喘中的临床疗效确切,复发率低,值得推广。     

徐荣谦教授运用大青龙汤合银翘散治疗小儿时行感冒经验

针对小儿时行感冒的临床特点和病因、病机,徐荣谦教授提出疫寒杂邪为其致病因素,清疫散寒为其基本治则,临床运用大青龙汤合银翘散治疗小儿时行感冒收效显著。     

胰岛素粉雾剂与注射剂治疗2型糖尿病的效果分析

目的：探讨胰岛素粉雾剂治疗2型糖尿病的临床效果。方法选取2012年1月~2014年4月本院收治的120例2型糖尿病患者为研究对象,将患者随机分成观察组与对照组,每组各60例。对照组患者给予胰岛素注射剂皮下注射治疗,观察组患者给予胰岛素粉雾剂吸入治疗,比较两组患者的血糖控制及不良反应的发生情况。结果观察组空腹血糖、餐后2h血糖以及糖化血红蛋白水平较对照组均明显下降,低血糖反应的发生率也明显低于对照组（P〈0.05）。结论胰岛素粉雾剂治疗2型糖尿病不仅能够有效控制患者血糖水平,提高临床效果,还能有效降低低血糖反应的发生率,提高临床治疗的安全性,是临床治疗2型糖尿病的有效药物,值得临床推广应用。     

布拉酵母菌预防新生儿感染性肺炎继发腹泻的临床研究

目的 探讨布拉酵母菌预防新生儿感染性肺炎继发腹泻的临床疗效。方法 2012年2月—2014年2月延安市妇幼保健院收治的感染性肺炎新生儿108例,随机分为治疗组（54例）和对照组（54例）。两组患者均给予相应的抗生素治疗,同时给予补液、补充维生素等支持治疗。治疗组患儿在服用抗生素的同时给予口服布拉酵母菌,125 mg/次,2次/d。对两组出现腹泻的患儿均口服蒙脱石散,1.0 g/次,3次/d,并且补液,直至治愈。两组均持续治疗14 d。治疗后,对两组患儿的临床疗效进行评价,同时对其细胞免疫学指标进行检测。结果 治疗组和对照组的总有效率分别为94.44%、75.93%,两组比较差异有统计学意义（P<0.05）。治疗组腹泻的发生率为38.89%,对照组为61.11%;治疗组腹泻发生时间明显晚于对照组,每天腹泻次数少于对照组,腹泻的持续时间也明显短于对照组,两组比较差异有统计学意义（P<0.05）。治疗后,治疗组的CD4⁺﹑CD4⁺/CD8⁺计数较治疗前显著升高;治疗组CD8⁺计数均较治疗前显著降低,治疗前后差异有统计学意义（P<0.05）,治疗组这些观察指标与对照组相比差异有统计学意义（P<0.05）。结论 布拉酵母菌用于预防新生儿感染性肺炎继发腹泻具有良好的临床效果,能够提高机体的免疫力,值得临床推广应用。     

不同冻干保护剂对利巴韦林冻干粉针的影响研究

目的研究Emprove低内毒素蔗糖、无水乳糖、Emprove低内毒素葡萄糖、Emprove低内毒素甘露醇、Emprove低内毒素山梨醇、Emprove低内毒素氯化钾、Emprove低内毒素甘氨酸7种不同类型常用冻干保护剂对利巴韦林冻干粉针性能的影响。方法以外观和复溶效果为指标,考察了预冻时间、冻干保护剂用量、冻干时间的影响。测定了空白粉针剂和利巴韦林粉针剂冻干后含水量、p H值和利巴韦林质量分数。结果以无水乳糖为冻干保护剂,预冻时间6 h,冻干时间9 h,保护剂用量4%;以Emprove低内毒素氯化钾为冻干保护剂,预冻时间9 h,冻干时间9 h,保护剂用量4%;以Emprove低内毒素甘露醇为冻干保护剂,预冻时间6 h,冻干时间6 h,保护剂用量4%;以Emprove低内毒素甘氨酸为冻干保护剂,预冻时间12 h,冻干时间9 h,保护剂用量4%。所得冻干粉针外观饱满、平整,迅速、完全复溶。结论无水乳糖、Emprove低内毒素氯化钾、Emprove低内毒素甘露醇、Emprove低内毒素甘氨酸4种冻干保护剂更适合制备利巴韦林冻干粉针,可为水溶性药物冻干粉针剂的制备提供了参考。     

A Study on the Antidepressant Effect of Danzhi xiaoyao Powder

Background

Danzhi Xiaoyao Powder is used for regulating qi and blood, soothing liver-qi stagnation and adjusting the function of internal organs in the clinical. The objective of this paper was to study the antidepressant effect of Danzhi Xiaoyao Powder.

Methods

Experimental animals were grouped and given different doses of xiaoyao powder and Fluoxetine Hydrochloride tablets respectively. Mouse models of behavioural despair such as tail suspension test and forced swimming test were used to evaluate the antidepressant effect of Xiaoyao.

Results

Danzhi Xiaoyao Powder could shorten the immobility time of mice in the tail suspension and forced swimming despair models, and had a significant effect on them, while having no significant effect on locomotor activity in mice.

Conclusion

Danzhi Xiaoyao Powder has a good antidepressant effect. The material basis and physiological and biochemical mechanisms of its action should be determined by further study.