八味地黄汤各药味对附子生物碱影响的电喷雾质谱研究

目的以八味地黄汤为研究对象,考察各药味对附子生物碱的影响规律,揭示附子配伍组方的科学内涵,为科学阐释八味地黄汤配伍规律提供化学依据。方法利用电喷雾质谱技术分别对八味地黄汤,附子加山茱萸、山药、肉桂、丹皮、泽泻、地黄、茯苓共煎液以及次乌头碱加山茱萸、山药共煎液进行系统考察。结果次乌头碱加山药、山茱萸,附子加山药等共煎液中次乌头碱含量较高;八味地黄汤、附子加山茱萸共煎液中次乌头碱含量较低;肉桂、丹皮、泽泻、地黄、茯苓等对附子生物碱的影响不明显。结论八味地黄汤中乌头碱和中乌头碱水解和溶出比较完全,次乌头碱的含量较少,次乌头碱的减少是由于水解以及溶出受到抑制导致。其中山茱萸对制附子起减毒作用,其减毒机制主要是抑制了制附子中次乌头碱的溶出。而山药则能导致附子毒性增加。     

以血清药理学方法研究黄连解毒汤对小鼠的药效动力学

目的以含药血清对小鼠肝匀浆体外生成丙二醛(M DA)的抑制作用为指标,研究黄连解毒汤及单味药水煎剂的药效动力学。方法测定小鼠ig黄连解毒汤及单味药所得含药血清对M DA生成作用的经时过程,并考察黄连解毒汤含药血清、单味药煎剂及小檗碱、黄芩苷抑制M DA生成作用的剂量-效应关系。结果小鼠ig黄连解毒汤、黄连、黄芩、黄连 黄芩后,含药血清均可抑制体外肝匀浆M DA生成,且作用呈剂量依赖性。各药的Em ax分别为86%、76%、79%及81%;tm ax分别为180、120、5及15 m in;其煎剂对M DA生成抑制作用呈剂量依赖性;小檗碱在0.12~10 ng/mL无明显的抑制作用;黄芩苷在1.11~10μg/mL有抑制作用,且作用亦呈剂量依赖性。结论黄连解毒汤体内抗氧化作用强于各单方。黄芩苷可能是黄芩药液和含药血清中的抗氧化成分之一,而小檗碱可能与含药血清抗氧化作用无关;小鼠ig黄连解毒汤及其单味药所得含药血清对M DA生成呈时间和剂量依赖性。     

补肾方含药血清促进PC12细胞增殖及其拮抗谷氨酸神经毒作用的研究

目的:运用中药血清药理学方法研究补肾复方含药血清(BS)促进体外培养的PC12细胞增殖及其拮抗谷氨酸兴奋性神经毒损伤的作用.方法:以MTT法观察BS对体外培养的PC12细胞活力的影响及其拮抗谷氨酸损伤的作用,采用流式细胞术观察BS对谷氨酸所诱导PC12细胞凋亡的影响.结果:BS含药血清可以增强PC12细胞活力,拮抗谷氨酸的兴奋性神经毒作用且在20%浓度时作用最强,并且能够抑制谷氨酸诱导的PC12细胞早期凋亡.结论:补肾方BS可能具有神经保护作用.     

平肝熄风汤对脑出血大鼠海马细胞色素C氧化酶活性和细胞凋亡影响同步研究

目的 从神经元线粒体能量代谢和细胞凋亡的角度探讨中药复方的平肝熄风汤对脑出血神经元损伤的保护作用机制。方法 采用Ⅶ型胶原酶诱导大鼠脑出血模型，以组织化学方法结合灰度值半定量测定细胞色素C氧化酶(cytochromc c oxidase，CCO)活性，采用Tunel法检测细胞凋亡结果造模12h，脑出血大鼠海马CA1区CCO活性较假手术组明显降低(P＜0.01)，凋亡细胞较假手术组明显增多(P＜0．01)随后均呈进行性加重。用平肝熄风汤治疗后町维持其CCO活性，减少细胞凋亡。结论 脑出血神经元损伤与神经元CCO活性降低及细胞凋亡有关平肝熄风汤能维持线粒体关键酶CCO活性，改善细胞有氧代谢，减少细胞凋亡，此可能为本方对脑出血继发性神经元损伤保护机制之一。     

助黄汤对小鼠胚泡着床数的影响

目的:探讨助黄汤对小鼠胚泡着床数的干预作用。方法:选用8～10周龄昆明种雌性小鼠,分别给予不同剂量的助黄汤,以及克罗米芬、助黄汤加克罗米芬,灌胃4d,于妊娠第8d处死小鼠,观察平均着床胚泡数,并进行了毒性实验。结果:助黄汤组平均着床胚泡数明显高于克罗米芬组及正常组,且药理毒性实验为阴性。结论:助黄汤能提高小鼠胚泡着床数,可间接反应该药有可能促进卵泡发育,增加排卵数量及卵的受精数目,增强小鼠的生殖功能的作用;且无毒副作用。     

Intervention Effects of Jianpi Liqi Huoxue Decoction (健脾理气活血汤) on Lipid Peroxidative Liver Injury by Alcohol

Objective: To study the intervention effects of Jianpi Liqi Huoxue Decoction ( 健脾理气活血汤, JLHD) on lipid peroxidative liver injury induced by alcohol. Methods: The rat alcoholic model of liver disease (ALD) induced by Lieber-DeCarli liquid diet was established. Thirty-two male SD rats were randomly divided into 4 groups: the normal group ( n = 5), the control group ( n = 9), the model group ( n = 9) and the JLHD group (n = 9). From the 4th week after modeling, the rats were given JLHD or distilled water by gastrogavage respectively, and the samples of blood and liver tissues were taken out from the rats for determination by the end of the 8th week. The hepatic pathological changes were observed with HE staining; the liver injury related indices, including activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, γ-glutamyl transpeptidase (γ-GT) activity and triglyceride (TG) content in liver tissues, as well as the lipid peroxidation related indices, including malonaldehyde (MDA) content and nitric oxide synthase (NOS) activity in liver tissue, serum Fe2 level, and the anti-peroxidation capacity related indices, including superoxide dismutase (SOD) activity, glutathion ( GSH) content and reactive oxygen species (antiROS) activity in liver tissues were determined. Results: ( 1 ) There were obvious figures of fatty degeneration and inflammatory infiltration in liver tissues of the model group. As compared with the control group, in the model group, the activity of ALT and AST, and Fe2 content in serum, γ-GT and NOS activity, TG and MDA content in liver tissues were significantly higher ( P＜0.01 ), while the activity of SOD, GSH and anti-ROS in liver tissues were significantly lower ( P＜0.01 ). (2) The fatty degeneration and inflammatory infiltration of liver tissues in the JLHD group were significantly lessen as compared with those in the model group; and the abnormalities of all the indexes revealed in the model rats were restored to certain extent in the JLHD group,and especially significant were the levels of ALT activity, MDA content and Fe2 , which were nearly normal.Conclusion: JLHD has significant effects against alcoholic liver injury, the acting mechanism of which is likely to be related with its anti-lipid peroxidative effect.     

活血通脉汤防治全膝关节置换术后深静脉血栓33例

目的探讨自拟中药活血通脉汤预防全膝关节置换术(TKA)后深静脉栓塞的效果。方法33例住院患者,均予以全膝关节置换术后第一天给予活血通脉汤口服每日1剂,同时进行CPM机功能锻炼和配合足底和下肢静脉泵,手术后7天做下肢彩色多普勒检查,观察血栓形成与否。结果8侧下肢彩色多普勒检查显示深静脉形成。结果33例患中6例8侧出现DVT占18·6%;比较无任何预防措施的TKA术后DVT发生率有明显降低。所有病例均无肺栓塞临床表现。结论利用中药活血通脉汤防治人工膝关节术后深静脉血栓形成有效。     

当归六黄汤加减对32例混合痔术后盗汗患者临床疗效观察

[目的]探讨当归六黄汤加减对混合痔术后盗汗的疗效。[方法]对32例混合痔术后盗汗患者采用当归六黄汤加减。[结果]治愈11例(占34·4%),显效16例(占50·0%),有效5例(占16·6%)。[结论]当归六黄汤能有效治疗混合痔术后盗汗。     

通淋活血法治疗输尿管结石临床疗效观察

泌尿系统结石属临床常见病、多发病,患病率约1%~5%,治疗后易复发[1]。我们自拟通淋活血汤Correspondence to:A-KANG GE.E-mail:xinhuayiyuan@126.com治疗输尿管结石,临床疗效明显,兹报道如下。1资料与方法1.1一般资料2003年6月~2005年6月,选择来我院中医科门诊治疗,且中医辨证属下焦湿热、下焦瘀滞的输尿管结石患者共60例(所有患者均经B型超声检查、X线摄片或静脉肾盂造影确诊为输尿管结石)。60例患者随机分为通淋活血汤治疗组和复方金钱草治疗组。通淋活血汤治疗组30例,其中男24例,女6例;年龄25~66岁,平均42.5岁,病程1周~16个月,平均7…     

软坚消瘿汤治疗慢性淋巴细胞性甲状腺炎的临床研究

目的观察软坚消瘿汤治疗慢性淋巴细胞性甲状腺炎的临床疗效。方法80例慢性淋巴细胞性甲状腺炎患者随机分为软坚消瘿汤治疗组和西药治疗组,每组40例。治疗16周后,检测两组患者甲状腺功能及血清抗甲状腺自身抗体滴度水平,并进行临床疗效评价。结果软坚消瘿汤治疗组总有效率92.5%,西药治疗组总有效率60.0%,两组比较差异有统计学意义。两组患者经治疗后,血清游离三碘甲状腺原氨酸、游离甲状腺素水平均较治疗前明显升高,差异有统计学意义;而血清促甲状腺素水平、血清抗甲状腺球蛋白抗体及抗甲状腺微粒体抗体滴度水平则均较治疗前明显下降,差异有统计学意义。结论软坚消瘿汤具有较好的软化、缩小肿大甲状腺及其结节的作用,可以提高机体的免疫功能、减轻甲状腺的自身免疫反应、促进甲状腺细胞功能的恢复。