许建中教授治疗肺间质纤维化学术经验

许建中教授认为,肺间质纤维化的主要病机为本虚标实,"痰"、"瘀"为标,肺肾两虚为本,治疗上应标本同治,既注重祛血瘀、化痰浊、通肺络,同时兼顾补肺益肾,益气养阴,此外,少佐清热解毒之品在治疗肺纤维化中也起到了不可忽视的作用。     

许润三学术思想初探

许润三教授是全国名老中医药专家之一,对于妇科疑难病的治疗有独到之处。学术思想主要有以中医为本,重视辨证论治,但不排斥西医,主张辨病辨证相结合。许教授临证时善于四诊合参,尤其重视舌脉。在妇科疾病的辨证治疗中,注重调养肝肾,认为肾为经孕之本,肝肾密切相关。治疗上善用经方,并勇于创新,用药力求药少力专。     

高效液相色谱法测定瑞巴匹特血浆浓度

目的 :建立测定血浆内瑞巴匹特浓度的高效液相色谱法。方法 :KromasilC18色谱柱 ,甲醇 -0 .0 5mol·L- 1磷酸二氢钾 (55∶45,pH3.0 )为流动相 ,并由序贯单纯形法确定此为最佳配比点 ,流速 1 .0ml·min- 1,检测波长 2 30nm。结果 :瑞巴匹特在 1 5～ 1 2 0 0 μg·L- 1范围内 ,血浆药物浓度C与色谱峰高H呈现良好线性相关 ,血浆中瑞巴匹特平均相对回收率为(97.5± 0 .5) %(n =5) ,平均绝对回收率为 (90 .3± 1 .8) %(n =5) ;平均日内和日间RSD分别为 4.6 8%和 5.6 6 %(n =5) ;方法对血浆瑞巴匹特最低检出浓度为 2 μg·L- 1。结论 :本方法适于血浆中瑞巴匹特的测定。     

Double minute chromosomes in a case of acute myeloblastic leukemia

A case of acute myeloblastic leukemia is reported in which, at diagnosis, 50% of the cells in bone marrow and blood contained double minutes (DMs). These cells disappeared from the marrow and blood following chemotherapy. Three postrelapse samples have remained negative for DMs. The relationship between DMs, homogeneous staining regions, and malignancy is discussed.     

Development and optimisation of a radioimmunoassay for plasma captopril

A specific and sensitive radioimmunoassay has been developed to monitor plasma levels of captopril, the first orally active angiotensin-converting enzyme inhibitor. Because of the reactive nature of the captopril thiol group, captopril was measured as the captopril-N-ethylmaleimide complex (captopril-NEM). Accuracy studies, using samples with known added concentrations of captopril, were satisfactory (r = 0.95, p less than 0.001, y = 0.97x - 2.02), and the radioimmunoassay results compared well with those determined by a gas chromatography/mass spectrometric method (r = 0.98, p less than 0.0005, y = 1.2x - 19). The minimum detection limit of the assay was 2 micrograms captopril/litre plasma.     

Activities of Biapenem against Mycobacterium tuberculosis in Macrophages and Mice

Objective To assess the activities of biapenem against multidrug-resistant and extensively drug-resistant Mycobacterium tuberculosis. Methods Biapenem/clavulanate(BP/CL) was evaluated for in vitro activity against Mycobacterium tuberculosis(Mtb) multidrug-resistant(MDR) isolates, extensively drug-resistant(XDR) isolates, and the H37 RV strain. BP/CL activity against the H37 Rv strain was assessed in liquid cultures, in macrophages, and in mice. Results BP/CL exhibited activity against MDR and XDR Mtb isolates in liquid cultures. BP/CL treatment significantly reduced the number of colony forming units(CFU) of Mtb within macrophages compared with control untreated infected macrophages. Notably, BP/CL synergized in pairwise combinations with protionamide, aminosalicylate, and capreomycin to achieve a fractional inhibitory concentration for each pairing of 0.375 in vitro. In a mouse tuberculosis infection model, the efficacy of a cocktail of levofloxacin + pyrazinamide + protionamide + aminosalicylate against Mtb increased when the cocktail was combined with BP/CL, achieving efficacy similar to that of the positive control treatment(isoniazid + rifampin + pyrazinamide) after 2 months of treatment. Conclusion BP/CL may provide a new option to clinically treat MDR tuberculosis.     

Di-2-Ethylhexyl Phthalate Induces Ovarian Toxicity and Alters Protein Expression of Hormone-regulated Receptors in Rats

<正>Phthalates are multifunctional compounds that are used in various commercial and industrial products, such as food packagings, toys, cosmetics, building materials, and personal care products[1-3], Di(2-ethylhexyl) phthalate(DEHP), one of the most commonly used phthalates, is ubiquitously present in humans[4]. Studies have shown that DEHP has