毫米波与红外线和紫外线联合治疗烧伤创面的临床观察

目的探讨毫米波、红外线、紫外线联合应用对烧伤的治疗效果。方法将80例深Ⅱ度烧伤患者随机分为4组:毫米波治疗组(A组)、红外线治疗组(B组)、紫外线治疗组(C组)、联合治疗组(D组),分别采用毫米波、红外线、紫外线及联合应用治疗烧伤创面,观察临床效果。结果治疗14d后对4组疗效进行比较,D组与A、B、C组疗效间差别均有统计学意义(P<0.05),A、B、C3组比较疗效间差别无统计学意义(P>0.05)。结论3种仪器联合应用能使深Ⅱ度烧伤创面的愈合加快,疗效明显优于单独应用毫米波、红外线、紫外线。     

低温联合紫外照射增强苦参碱诱导Colo320细胞凋亡作用的研究

目的从细胞凋亡角度探讨低温、辐射、中药综合治疗大肠癌的机制。方法采用Hoechst33258荧光染色技术,检测低温、紫外线两种物理因素及中药苦参碱分别及联合运用时的Colo320细胞凋亡率。结果倒置显微镜下均可以观察到细胞凋亡情况。经单纯低温-4℃,0℃,4℃处理,凋亡率为30.98%、31.72%、33.06%;经单纯15W紫外灯下50、100、150cm处照射,凋亡率为35.56%,22.69%,15.08%;经低温与紫外照射共同影响,其凋亡率为42.31%;经低温联合紫外照射及苦参碱共同作用,凋亡率达48.17%。结论Co-lo320细胞凋亡反应在低温、紫外照射及苦参碱的共同影响下,其诱导凋亡作用明显提高。     

黄芩苷提取工艺研究

目的:对现有黄芩苷提取工艺中的水提、酸沉、水洗、醇洗各参数进行优化,为今后黄芩苷的提取和纯化提供参考。方法:以黄芩苷含量为指标,通过正交设计实验,对影响水提、酸沉、水洗、醇洗工艺的因素进行考察,并进行方差分析。结果:确定了提取黄芩苷的最佳工艺条件。结论:该提取工艺合理,黄芩苷收率高,质量易于控制。     

PMA诱导节律基因表达对细胞紫外线损伤的影响

目的研究诱导NIH3T3细胞节律基因表达对细胞紫外线损伤的影响。方法用体外节律诱导剂乙酰肉豆蔻佛波酯诱导NIH-3T3细胞节律基因表达。分别选择mPer2基因表达的谷值和峰值时刻对细胞进行紫外线照射,以非诱导节律的单纯照射组为对照,通过流式细胞术检测细胞凋亡和细胞周期分布,平板克隆形成试验检测细胞增殖情况。结果mPer2基因表达峰值时进行紫外线照射的细胞,与mPer2基因表达谷值时和非诱导节律紫外线照射细胞比较,增殖能力强、凋亡率低、G1期阻滞明显。结论节律基因诱导的NIH3T3细胞的紫外线损伤减小,并与mPer2的基因表达相关,其保护作用可能与mPer2基因过表达有关。     

A Bacterial DNA Repair Test Evaluating the Genotoxicity of Light Sources

A DNA repair test was used in order to assess its applicability for detecting the genotoxicity of sunlight and of the light emitted by halogen lamps and fluorescent lamps. This experimental system compares the lethality of test agents in the Escherichia coli wild-type WP2 and its isogenic counterparts lacking, either individually or in combination, various DNA repair mechanisms. DNA repair-deficient strains included WP2uvrA (uvrA^-), WP67 (uvrA^- polA^-), CM561 (lexA^-), CM571 (recA^-), WP100 (uvrA^- recA^-), and CM871 (uvrA^- recA^- lexA^-). All light sources produced a substantial killing of repair-deficient strains, with a maximum activity in the triple mutant CM871, at doses that did not affect survival of the wild type. The genotoxicity of uncovered quartz halogen bulbs was particularly potent, compared to fluorescent lamps and sunlight. Moreover, the mechanisms involved in repairing the DNA damage induced by halogen lamps were similar to those of a 254 nm UV source. The spectrum of genetic damage produced by sunlight and fluorescent lamps was conversely more comparable to that of a 365 nm UV source. These data demonstrated a harmful emission of appreciable amounts of genotoxic far-UV wavelengths by halogen lamps, thereby confirming our previous results in the his^-Salmonella typhimurium mutagenicity test. Genotoxicity of halogen lamps could be easily prevented in both experimental systems by suitable glass or plastic covers. Compared to the mutagenicity end point, the differential lethality end point provided even more clear-cut results in detecting the DNA-damaging ability of all light sources. Moreover, parallel assays provided evidence that the bacterial DNA repair test was far more sensitive than the mutagenicity test in evaluating the genotoxicity of the light produced by halogen lamps. On the whole, the DNA repair test in E. coli is even simpler and faster (24 vs. 48 h) than the Salmonella mutagenicity test, and compares favorably in terms of sensitivity to genotoxic light sources.     

Synergistic effect of donor pretreatment with 8-methoxypsoralen and ultraviolet irradiation of the graft plus azathioprine and prednisolone therapy in prolonging rat renal allograft survival

Summary Pretreatment of the kidney donor with 8-methoxy-psoralen (8-MOP) and direct longwave ultraviolet (UVA) irradiation of the kidney graft (PUVA therapy) significantly prolonged survival in allogeneic recipients. 40% of the recipients survived more than 100 days with normal transplant function. The addition of standard clinical immunosuppressive agents azathioprine and prednisolone (both at dosages of 15 mg/kg body weight/day for 21 days) to the PUVA therapy further improved graft survival rate, with a recipient survival rate of 62.5%. The two drugs alone were less effective in prolonging graft survival rate (28.5%). A synergistic effect of PUVA therapy and standard immunosuppressive treatment with azathioprine and prednisolone was demonstrated. This suggested a possible clinical application of this type of immunosuppression and immunoregulation.     

紫外光对离体羊晶状体内TNT的光化诱导作用

为探讨紫外光在三硝基甲苯（TNT）致晶状体损伤中的作用，观察了紫外光对离体羊晶状体内TNT光化反应的诱导作用，以及TNT光化反应物的致高铁血红蛋白形成作用。结果：随照射剂量增加羊晶状体内TNT光化反应产物同步增加；TNT光化反应比TNT原形有更强的致高铁血红蛋白形成作用。结论：紫外光照射对TNT致晶状体损伤作用有重要影响。     

万乃洛韦联合紫外线照射治疗玫瑰糠疹疗效观察

目的观察万乃洛韦联合紫外线照射治疗玫瑰糠疹的疗效。方法将93例患者随机分为两组,治疗组60例口服万乃洛韦300mg 2次/d,连续10天;对照组33例口服左西替利嗪10mg 1次/d,维生素C 0.2g 3次/d,葡萄糖酸钙1.0g 3次/d,连续10天。两组均同时隔日一次局部紫外线照射。结果治疗组有效率为83.33%,对照组为57.57%,二者差异有显著性(P<0.01)。结论万乃洛韦联合紫外线照射治疗玫瑰糠疹的疗效好。     

四膜虫对8-MOP处理的生长反应—真核原生生物四膜虫DNA损伤修复机理的研究(Ⅰ)

本文研究了四膜虫在8—MOP和紫外线(波长为365nm)照射联合处理以后细胞的生长反应。我们观察到被处理的细胞或暂时性受抑(<1μg—8MOP/ml细胞悬液)或永久性受抑(>1μg 8—MOP/1ml)细胞悬液)。暂时性受抑的细胞,在经过一定的迁延期以后可恢复其生长能力,其迁延期长短与8—MOP的剂量有关。     

“派莎”防晒乳膏对中波紫外线损伤人角质 形成细胞的保护作用

［摘要］ 目的探讨“派莎”防晒乳膏对中波紫外线（UVB）损伤人角质形成细胞（HKC）的保护作用及作用机制。方法用“派莎”防晒乳膏对HKC进行预处理24 h，采用不同强度的UVB 照射体外培养的HKC，24 h 后以噻唑蓝（MTT）法检测细胞生存率，用酶联免疫吸附（ELISA）法检测上清液中白细胞介素（IL 8）浓度，以酶联免疫吸附实验检测细胞上清液中肿瘤坏死因子（TNF） α的分泌量。结果经UVB照射后，细胞损伤程度与UVB照射剂量有关，“派莎”防晒乳膏可提高UVB照射后HKC的生存率，减少细胞因子TNF α的分泌。UVB 照射可使HKC 分泌IL 8 增加，“派莎”防晒乳膏组IL 8 浓度与对照组比较差异有显著性（P＜0.05）。结论UVB对HKC有损伤作用，且与照射强度相关，“派莎”防晒乳膏对HKC具有光保护作用。抑制TNF α和IL 8的释放，提高UVB照射后HKC的生存率，可能是其保护作用机制之一。