地高辛、维生素B1混合粉末在常温下的稳定性考察

目的：研究地高辛与维生素B1混合粉末在常温下的稳定性。方法：在25℃条件下，采用紫外分光光度法，测定其含量、重量在10、20、30、40、50天的变化情况。结果：在25℃下，混合粉末在30天前基本稳定。结论：地高辛、维生素B1混合粉末保存不应大于30天。     

姜黄素对长波紫外线所致DNA损伤的拮抗作用

目的　观察长波紫外线引起人表皮样癌细胞 DNA链断裂损伤的情况 ,以及姜黄素对这种损伤的拮抗作用。方法　用长波紫外线照射细胞 ,用单细胞凝胶电泳法检测 DNA损伤。结果　长波紫外线剂量依赖性地诱发表皮细胞的 DNA链断裂损伤 ,损伤的高峰出现在照射后 1h。而姜黄素可以拮抗长波紫外线引起的DNA链断裂 ,表现为彗星细胞百分比的降低和彗星尾巴长度的缩短 ,并有量效关系。结论　姜黄素可以预防长波紫外线照射引起的皮肤细胞 DNA链断裂损伤。     

紫外分光光度法测定加替沙星眼凝胶中加替沙星的含量

目的:建立加替沙星眼凝胶中加替沙星的含量测定方法.方法:采用紫外分光光度法测定,测定波长292 nm.结果:加替沙星在2.4～6.4μg·ml-1浓度范围内呈良好的线性关系,平均回收率99.77%,RSD 0.22%.结论:本法操作快速简便,结果准确,适用于加替沙星眼凝胶制剂半成品含量的快速测定.     

紫外分光光度法测定盐酸川芎嗪氯化钠注射液中盐酸川芎嗪含量

目的:建立盐酸川芎嗪氯化钠注射液中盐酸川芎嗪的含量检测方法.方法:采用紫外分光光度法,检测波长为295nm.结果:盐酸川芎嗪在3.0～15.0μg·ml-1范围内呈良好线性关系(r=1.0000,n=5),回收率为100.3%,RSD为0.65%(n=5).结论:本法操作简便、快捷,结果准确,尤适用于药厂、医院对该制剂半成品的质量控制和快速分析.     

中波段紫外线对角质形成细胞凋亡的诱导和钙信号的影响

目的　观察中波段紫外线 (UVB)照射诱导人永生化角质形成细胞 (HaCaT细胞 )的凋亡和对细胞静息Ca2 + 及thapsigargin和adrenaline引起的Ca2 + 运动影响。方法　采用四甲基偶氮唑盐 (MTT)染色法分析受UVB照射后细胞存活率的变化 ,用流式细胞术、琼脂糖凝胶电泳和Hoechst332 5 8核染色法观察UVB对HaCaT细胞凋亡的影响 ,采用Fura 2 /AM荧光分光光度法测定胞浆游离Ca2 + 浓度的变化。结果　在 1 2 5～ 6 2 5J·m-2 的UVB照射剂量之间和照射后培养时间 2 4h内的条件下 ,UVB照射以剂量和时间依赖的方式抑制细胞的存活率 ;在照射后培养时间 1 8h内以剂量依赖的方式诱导细胞凋亡 ;2 5 0J·m-2 UVB照射后 ,细胞的静息钙迅速下降 ,在照射后第 6h静息钙降至最低 ,且thapsigargin(1 0μmol·L-1 )和adrenaline(1 0 μmol·L-1 )所引起的钙释放及由此产生的钙内流也随照射后培养时间的延长而逐渐减少。结论　UVB照射可诱导HaCaT细胞的凋亡及引起细胞钙稳态的失衡。     

臭氧·紫外线器械消毒柜消毒效果与影响因素的实验研究

目的探讨臭氧*紫外线消毒柜的机器性能和消毒效果与影响因素. 方法按卫生部<消毒技术规范>测定开机不同时间消毒柜中臭氧浓度、工作环境空气中臭氧浓度和消毒柜中臭氧残留浓度,以及消毒柜对不同微生物的杀灭效果. 结果按消毒柜自动程序开机20min柜内臭氧浓度达253.69mg/m3,柜中平均紫外线照射强度为1 862μW/cm2;消毒柜开机工作35min对不锈钢载体上金黄色葡萄球菌、大肠埃希菌、白色念珠菌杀灭率达100%;开机40min对不锈钢载体上枯草杆菌黑色变种芽胞杀灭率达100%,对染于手术剪和镊子上的金黄色葡萄球菌杀灭率达99.99%,消毒效果可靠而且安全. 结论肯格王牌臭氧*紫外线消毒柜容量大、自动、安全、消毒效果可靠、适用范围广泛的医疗器械消毒产品.     

Minimal and maximal incidence rates of skin cancer in Caucasians estimated by use of sigmoidal UV dose–incidence curves

BackgroundSigmoidal (S-shaped) dose–cancer incidence relationships are often observed in animal bioassays for carcinogenicity. Ultraviolet (UV) radiation is an established skin carcinogen. The aim of this study is to examine if S-shaped curves describe the relationship between solar UV doses and skin cancer incidences, and if such relationships can be used to estimate threshold levels of non-carcinogenic UV exposure, as well as maximal incidence rates.MethodsWe studied the incidence rate–annual erythema-effective UV dose relationship for squamous cell carcinoma (SCC), basal cell carcinoma (BCC) and cutaneous melanoma (CM) among different Caucasian populations in Europe, Australia and New Zealand.ResultsOur analysis indicates that S-shaped associations describe the data well (P < 0.0001). The age-adjusted incidence rates for cases expected to be due to other causes than solar UV exposure (at zero UV dose) were found to be around 0.6, 9.7 and 4.0 per 100,000 for women in 1997–2007 for SCC, BCC and CM, respectively, and around 1.2, 14.3 and 2.6 per 100,000 for men. The analysis indicates that SCC, BCC and CM have maximal incidence of 361 ± 24, 1544 ± 49 and 36 ± 4 per 100,000 for women, and 592 ± 35, 2204 ± 109 and 50 ± 4 per 100,000 for men.ConclusionsBetween 89 and 95% of the annual CM cases, around 99.8% SCC and 99.4% BCC cases are caused by solar UV exposure. The analysis did not identify any “safe” UV dose below which the risk for skin cancer was absent. Avoidance of UV radiation has a potential to reduce the incidence of skin cancer in fair-skinned population.     

An evaluation of the treatment of parapsoriasis with phototherapy

Whether parapsoriasis represents an early stage of T-cell cutaneous lymphoma is still the subject of controversy. We evaluated the efficacy of phototherapy in the treatment of parapsoriasis and its relation with TCCL. Patients diagnosed with parapsoriasis and treated with phototherapy PUVA or UVB-NB were selected. Between 1 to 8 years following treatment the evolution of their disease was evaluated. In 62 patients the cure rate was 79.3% and 17.2% showed improvement of the lesions. Only two patients developed full blown T-cell cutaneous lymphoma. Phototherapy is an excellent treatment for parapsoriasis, with high cure rates, regardless of the type of phototherapy employed. Of the 62 patients under study, parapsoriasis showed no general tendency to progress to T-cell cutaneous lymphoma.     

光疗治疗银屑病的研究进展

目前治疗银屑病的方法较多,但治疗效果令人满意的方法尚少。光疗是治疗银屑病的一种有效方法,与药物联合应用不仅可以提高疗效,还可减少不良反应的发生。现将近年光疗治疗银屑病的研究进展综述如下。