MicroRNA-21对食管鳞状上皮癌细胞TE-1生物学特性的影响

目的:食管鳞状上皮癌TE-1细胞中microRNA-21(miR-21)高表达,本研究探讨miR-21对TE-1细胞生物学特性的影响。方法:转染miRZip-21慢病毒,持久抑制TE-1细胞中高表达的miR-21,将由此获得的稳定细胞系命名为anti-miR-21细胞系,实时定量PCR方法验证,以TE-1细胞作为对照。细胞增殖实验检测anti-miR-21细胞增殖能力,Transwell侵袭实验观察细胞侵袭能力,Transwell迁移实验及划痕实验观察细胞迁移能力,克隆形成实验及细胞毒性实验分别观察放射线及药物敏感性变化。结果:Anti-miR-21细胞的相对增殖率较对照组TE-1细胞低,差异具有统计学意义(P<0.05)。Transwell侵袭实验及迁移实验显示穿过小室的anti-miR-21细胞较TE-1细胞分别减少51.97%及64.31%,差异具有统计学意义(P<0.05)。划痕实验显示anti-miR-21细胞较TE-1细胞的迁移率降低,差异具有统计学意义(P<0.01)。克隆形成实验分析显示anti-miR-21细胞的D0值(2.73Gy vs 3.60Gy)和Dq值(1.25Gy vs 2.75Gy)均低于对照组,差异具有统计学意义(P<0.05)。细胞毒性实验显示anti-miR-21细胞在顺铂及氟尿嘧啶各个梯度浓度下细胞存活率均降低,差异具有统计学意义(P<0.05)。结论:MiR-21影响食管鳞状上皮癌TE-1细胞的生物学特性。抑制miR-21表达后,降低了TE-1细胞的增殖、侵袭及迁移能力,同时增加了其对放射及化学药物的敏感性。MiR-21可能成为食管鳞癌治疗的潜在靶点。     

Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma

Transitional bladder carcinoma (BCa) is prevalent in developed countries, particularly among men. Given that these tumors frequently recur or progress, the early detection and subsequent monitoring of BCa at different stages is critical. Current BCa diagnostic biomarkers are not sufficiently sensitive for substituting or complementing invasive cystoscopy. Here, we sought to identify a robust set of urine biomarkers for BCa detection. Using a high-resolution, mass spectrometry-based, quantitative proteomics approach, we measured, compared and validated protein variations in 451 voided urine samples from healthy subjects, non-bladder cancer patients and patients with non-invasive and invasive BCa. We identified five robust biomarkers: Coronin-1A, Apolipoprotein A4, Semenogelin-2, Gamma synuclein and DJ-1/PARK7. In diagnosing Ta/T1 BCa, these biomarkers achieved an AUC of 0.92 and 0.98, respectively, using ELISA and western blot data (sensitivity, 79.2% and 93.9%; specificity, 100% and 96.7%, respectively). In diagnosing T2/T3 BCa, an AUC of 0.94 and 1.0 was attained (sensitivity, 86.4% and 100%; specificity, 100%) using the same methods. Thus, our multiplex biomarker panel offers unprecedented accuracy for the diagnosis of BCa patients and provides the prospect for a non-invasive way to detect bladder cancer.     

老年人肺结核的临床X线特点分析

目的：探讨并提高对老年人肺结核X线表现及特点的认识。方法：收集本院的86例年龄在60-76岁患者胸片与86例中青年肺结核胸片进行对照分析。结果：根据X线征象分析为：（1）多双侧肺发病，并且病变范围广泛；（2）病变性质往往为干酪性坏死；（3）常形成支气管播散；（4）易形成空洞，且空洞数目为多发；（5）并发症较多。结论：常规胸片仍是目前诊断老年肺结核首选的检查方法，结合临床和实验室资料可获得正确诊断。     

Validation of the Defining Characteristics of Altered Sexuality Patterns

Human sexuality is a complex and multifaceted phenomenon that encompasses physical, psychological, and social components. The nursing diagnosis altered sexuality patterns labels the human response to alterations in sexuality that occur as the result of various health problems. This validation of the defining characteristics of the diagnosis was based on a substantive theory derived from a qualitative grounded theory study. In the original study, data from 11 adult insulin-dependent diabetics living in a rural area of the Midwest were analyzed and relevant experiences conceptualized through the core concept of transforming and the supporting concepts of valuing self and meeting intimacy needs. The theory was then used as a framework to formulate specific defining characteristics for the nursing diagnosis. A single diagnosis of altered sexuality has emerged that encompasses the perception of change in sexuality as well as the change itself.     

A review of dramatherapy research in schizophrenia: methodologies and outcomes

Abstract

This article aims to examine all the different types of research studies in the existing literature regarding the impact of dramatherapy on patients with a schizophrenic disorder to provide a springboard for future advances in the application of dramatherapy as an adjunct therapeutic approach for this client group. Quantitative research approaches in the form of randomized clinical trials have proven to be feasible in dramatherapy when they aim at providing evidence of the improvement in the condition of patients with schizophrenia or of the diminution of their symptoms. Qualitative approaches explore the therapeutic processes through which dramatherapy practice can confront more effectively the schizophrenic psychopathology. Furthermore, theater-based research brings to light the creative side of patients with schizophrenia despite their psychopathology and promotes through action the discourse between patients “in role” and their audience in relation to the social stigma attached to people with schizophrenia.

Dieser Artikel zielt darauf ab, alle unterschiedlichen Arten der Untersuchung der Effekte von Dramatherapie bei Patienten mit einer schizophrenen Störung durchzugehen, um einen Fortschritt in der Anwendung von Dramatherapie als einem Hilfsprogramm für diese Patientengruppe zu ermöglichen. Quantitative Untersuchungen in Form von randomisierten klinischen Studien haben sich für Dramatherapie als möglich erwiesen, wenn sie auf der Basis einer Verbesserung des Zustandes der Schizophreniepatienten durchgeführt werden oder auf eine Verringerung ihrer Symptome abzielen. Qualitative Vorgehensweisen erforschen die therapeutischen Prozesse, durch die die Anwendung von Dramatherapie schizophrene Psychopathologie positiv beeinflussen kann. Darüber hinaus zeigen Theater-basierte Untersuchungen die kreativen Seiten von schizophrenen Patienten auf, die sie trotz ihrer bestehenden Psychopathologie haben und fördern den handlungsbezogenen Diskurs zwischen Patienten in einer Rolle und ihre Zuhörerschaft hinsichtlich des sozialen Stigmas, das Menschen mit Schizophrenie anhaftet.

Cet article vise à examiner les différents types de recherches dans la littérature sur l'impact de la thérapie par le théâtre (dramatherapy) pour les patients avec un trouble schizophrénique dans le but de fournir un tremplin pour des avancées futures dans l'application de la thérapie par le théâtre comme approche thérapeutique complémentaire pour ce groupe de patients. Les approches quantitatives, sous la forme d’études randomisées contrôlées, ont démontré leur faisabilité pour la thérapie par le théâtre lorsque celle-ci vise à fournir la preuve de l'amélioration de la condition des patients schizophrènes ou de la diminution de leurs symptômes. Les approches qualitatives explorent les processus par lesquels la pratique de la thérapie par le théâtre peut faire face plus efficacement à la psychopathologie schizophrénique. En outre, les recherches basées sur le théâtre éclairent le côté créatif des patients avec une schizophrénie et encouragent par l'action un discours entre le patient «?dans son rôle?» et son audience en relation avec la stigmatisation sociale attachée au personnes avec une schizophrénie.

Este artículo se propone examinar los diferentes tipos de investigaciones en la literatura existente sobre el impacto de la dramaterapia en pacientes con desorden esquizofrénico, a fin de proveer un trampolín para futuros avances en la aplicación de la dramaterapia como adjunto terapéutico para este grupo de clientes. Los enfoques de investigación cuantitativa en forma de ensayos clínicos randomizados han demostrado ser factibles en dramaterapia cuando buscan evidencia de mejoría en la condición de pacientes con esquizofrenia o en la disminución de sus síntomas. Los enfoques cualitativos exploran los procesos terapéuticos a través de los cuales la práctica de la dramaterapia puede hacer frente más eficazmente a la psicopatología esquizofrénica. Más aún, la investigación basada en el teatro trae a luz el aspecto creativo de los pacientes con esquizofrenia a pesar de su psicopatología y promueve, a través de la acción, el discurso entre pacientes “en el rol” y su audiencia, en relación con el stigma social que afecta a las personas con esquizofrenia.

Este artigo procura examinar todos os diferentes tipos de estudos de investigação existentes na literatura existente relativos ao impacto da terapia de drama nos pacientes com perturbaç[otilde]es de esquizofrenia, para assim providenciar uma plataforma para futuros avanços na aplicação desta terapia como uma abordagem terapêutica adicional para este grupo de pacientes. Os estudos de abordagens quantitativas com grupos clínicos aleatoriamente seleccionados têm-se mostrado serem viáveis para a terapia de drama quando o seu objectivo era fornecer evidência para melhorias nas condiç[otilde]es dos pacientes com esquizofrenia ou para a diminuição dos seus sintomas. As abordagens qualitativas exploram os processos terapêuticos através da prática da terapia de drama podem mais eficazmente abordar a psicopatologia esquizofrénica. Para além disso, estudos baseados no teatro evidenciam o aspecto criativo do paciente com esquizofrenia, apesar da sua psicopatologia, e promovem através da acção o discurso entre pacientes “a representar” e a sua audiência em relação ao estigma social associado às pessoas com esquizofrenia.

Questo articolo ha lo scopo di esaminare tutti i differenti tipi di studi di ricerca nella letteratura esistente per quanto riguarda l'effetto della dramma-terapia sui pazienti con schizofrenia, per fornire un trampolino di lancio per progressi futuri nell'applicazione della dramma-terapia come approccio terapeutico aggiuntivo per questo gruppo di pazienti.

I metodi di ricerca quantitativi, nella forma degli esperimenti clinici randomizzati, sono risultati essere fattibili nella dramma-terapia quando mirano a provare l'evidenza del miglioramento nei pazienti con schizofrenia o della diminuzione dei loro sintomi.

I metodi qualitativi esplorano i processi terapeutici con cui la pratica della dramma-terapia può confrontare pi[ugrave] efficacemente la psicopatologia schizofrenica.

Ancora, la ricerca basata sul teatro mette in luce il lato creativo dei pazienti con schizofrenia malgrado la loro psicopatologia e promuove, attraverso l'azione, il discorso tra i pazienti “nel ruolo” e il loro uditorio rispetto allo stigma sociale, attaccato alle persone con schizofrenia.

     

Acoustics characteristics of voice and vocal care in acting and other students

Based on voice‐history data, a χ² test was used to investigate the difference between students of acting (n = 45) and other students (n = 45). A t‐test was used to calculate the differences in acoustic parameters between the two groups. It was expected that students of acting spent significantly more time practicing voice exercises, took more acting instructions, and generally spoke more in larger rooms and did warm up exercises (p<.001). However, it was not expected that they smoked more than non‐professionals (p = .003), and that they drank alcoholic drinks as much as other students. Male students of acting had significantly lower ?₀ SD means (p = .015), which means that they had a more stable pitch throughout phonation. Students of acting also showed a significantly higher Harmonics‐to‐Noise Ratio (HNR) than other students (p = .001 for males; p = .01 for females). The data showed the importance of the appropriate use of voice, which reflected relatively good voice quality despite the bad living habits of the future professional voice users.     

云南省昭通市2005-2014年老年人HIV/AIDS流行特征分析

目的 了解云南省昭通市2005-2014年≥50岁老年人艾滋病的流行特点,为制定防治策略提供依据。方法 通过中国疾病预防控制信息系统艾滋病综合防治信息系统收集昭通市2005-2014年≥50岁老年人艾滋病疫情报告资料,对其基本情况、传播途径和流行趋势等进行分析。结果 昭通市2005-2014年累计报告≥50岁艾滋病病毒感染者/艾滋病病人(HIV/AIDS)540例,平均年龄(62.1±8.9)岁,男性67.8%(366/540),女性32.2%(174/540)。文化程度以文盲和小学为主,分别为42.2%(228/540)和40.4%(218/540);职业以农民为主为76.6%(414/540);婚姻状况为已婚者较多,占65.0%(351/540)。传播途径以异性接触传播为主,占93.3%(504/540);老年人HIV/AIDS主要分布在疫情较重、经济发达的县(区)。≥50岁HIV/AIDS占当年新报告HIV/AIDS比例逐渐增加,从2005年的1.7%上升到2014年的36.0%,差异有统计学意义(χ_趋势²=197.9,P<0.01);异性接触传播占当年报告比例从2005年的10.0%增加到2014年的37.0%,差异有统计学意义(χ_趋势²=119.5,P<0.01)。结论 昭通市老年人艾滋病疫情比较严重,应将老年人艾滋病防治工作纳入常规工作,根据老年人特点开展有针对性的综合干预服务。     

成都地区2009-2013年儿童人博卡病毒感染分析

目的 了解四川成都地区急性呼吸道感染住院患儿人博卡病毒(HBoV)的感染情况,分析其流行特征。方法 采集2009年10月-2013年10月年成都市某妇女儿童医院因呼吸道感染而住院的患儿鼻咽抽提物,提取核酸,用巢式PCR方法检测人博卡病毒;同时对流感病毒等其他7种呼吸道病毒进行检测,了解混合感染情况;用统计学软件对检测结果及病例信息进行分析。结果 在1842份标本中,HBoV检出率为6.84%(126/1842),与其他呼吸道病毒混合感染率为58.73%(74/126);患儿以下呼吸道感染为主,1~2岁检出率最高,为7.83%(108/1379);HBoV感染未见明显的季节性分布特点。结论 HBoV为成都地区儿童发热呼吸道症候群的主要病原之一,>2岁儿童检出率最高,且与其他病毒有较高的混合感染率。     

台州市黄岩区气传真菌孢子散布规律与气象因素关系

目的 探讨浙江省台州市黄岩区气传真菌孢子的散布规律及其与气象因素之间的关系,为防治气传真菌引起的过敏性变态反应疾病提供参考依据。方法 在浙江省台州市黄岩区采用连续24 h曝片法收集2012年9月1日-2013年08月31日气传真菌样品,并记录每日气象资料,分析气传真菌孢子的散布与季节、天气变化的消长规律。结果 共收集曝片336片,检出真菌孢子60余种共13 822个,其中黑孢子菌检出数最多(31.42%),其次为芽枝菌(23.66%)和毛霉菌(21.83%);3-10月空气中漂浮的真菌孢子较多,并呈双峰分布,3-5月和7-9月为高峰期;春季、夏季、秋季和冬季空气中检出真菌孢子数分别为(1 705.41±303.28)、(1 384.71±242.14)、(760.44±109.24)和(221.35±60.46)个,4个季节真菌孢子数差异有统计学意义(F=900.634,P < 0.001);日均气温 < 15 ℃时,空气中真菌孢子数量随温度升高增量明显,≥15 ℃真菌孢子数量逐渐趋于稳定;风速与气传真菌孢子数量存在反向增长的关系;气传真菌孢子数随着降雨量的增大呈现出先增多而后减少的趋势。结论 气传真菌孢子在空气中广泛存在,其数量与气温、风速和降雨量等气象因素有关。摄氏度     

Study of the Dynamic Model and Vibration Performance of Pot-Shaped Metal Rubber

Metal rubber has been extensively used in recent years due to its several unique properties, especially in adverse environments. Although many experimental studies have been conducted, theoretical research on metal rubber is still in its infancy. In this work, a dynamic model for the nonlinear characteristics of pot-shaped metal rubber is established on the basis of the asymmetric dynamic model of the wire rope shock absorber and the trace method model. In addition, the corresponding parameters in the model are identified based on the parameter-separation method. The theoretical hysteresis loop obtained using the model and the measured hysteresis loop agree with each other. The results show that the asymmetric dynamic model can better describe the asymmetric dynamic characteristics of pot-shaped metal rubber. Furthermore, a pot-shaped metal rubber vibration reduction system is built to further verify the correctness of the model. This study provides an experimental reference and a theoretical basis for the practical application of pot-shaped metal rubber in the field of three-dimensional vibration reduction.