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31.
Ken Yamaguchi Katsumi Aoyagi Ken-ichi Urakami Toyoharu Fukutani Noboru Maki Shigehiro Yamamoto Kotomi Otsubo Yoshio Miyake Tetsuro Kodama 《Cancer science》1995,86(7):698-705
Our previous study demonstrated that pro-gastrin-releasing peptide(31–98), or ProGRP, is a specific tumor marker in patients with small cell lung carcinoma (SCLC). Using a newly developed, highly sensitive enzyme-linked immunosorbent assay (ELISA) for ProGRP, we analyzed 1,446 samples including those obtained from 478 lung cancer patients to evaluate the clinical usefulness of this ELISA. Several properties indicated that ProGRP is a useful tumor marker for SCLC. First, ProGRP was specifically elevated in SCLC patients. In non-SCLC patients and patients with non-tumorous lung diseases, its serum level was very rarely elevated. Secondly, ProGRP was a reliable marker, in terms of the marked elevation of serum ProGRP levels in SCLC patients. Thirdly, serum ProGRP levels were elevated in SCLC patients even at a relatively early stage of this disease. Fourthly, changes in the serum ProGRP level showed an excellent correlation with the therapeutic responses in SCLC patients. Neuron-specific enolase (NSE) is accepted as a tumor marker of SCLC patients. With the aim of comparing ProGRP and NSE as tumor markers for SCLC patients, we measured serum NSE levels in all samples collected in the present study. We found that ProGRP was superior to NSE in terms of sensitivity, specificity and reliability. Therefore, we consider that ProGRP can play a major role as a clinical tumor marker for SCLC patients. 相似文献
32.
本文采用S-100蛋白为免疫学标记,对34例恶性肿瘤局部淋巴结内树突状细胞进行免疫组化定量研究。结果按S-100蛋白阳性细胞数目多少分为增多(7例)、减少(20例)及正常(7例)3组,统计学分析增多组均值(164.4个/mm^2)明显高于对照组(58.3个/mm^2);减少组均值(16.5个/mm^2)组显低于对照组;而正常组均值(69.8个/mm^2)与对照组无显著差异。 相似文献
33.
Anti-tumor necrosis factor (TNF) antibodies inhibit passively transferred experimental allergic encephalomyelitis (EAE) in SJL mice. The possibility that this occurs through interference in TNF's upregulation of endothelial cell adhesion molecules was investigated. Expression of both vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) on spinal cord vessels increased during EAE. The upregulation of VCAM-1 was markedly reduced or prevented by anti-TNF treatment. Leukocytic infiltration was 15-fold lower in anti-TNF-treated than diseased animals. Spinal cord endothelial expression of VCAM-1, though not ICAM-1 or fibronectin, positively correlated with the extent of T cell, B cell or monocyte infiltration in each animal. 相似文献
34.
甲型流流感病毒鼠肺适庆型75-39株治疗S37腹水型荷瘤小鼠,存活率达93.3%。体外实验观察流感病毒感染S37肿瘤细胞,到3天时S37细胞经胎盘蓝染色,100%的S37细胞死亡,而对照组肿瘤细胞染色率只有10%左右。 相似文献
35.
旋毛虫幼虫分泌物抗原在小鼠体内诱生肿瘤细胞毒因子的研究 总被引:6,自引:0,他引:6
本文应用L929细胞杀伤法,对注射旋毛虫肌肉期幼虫分泌物(L1ES)的小鼠血清进行了检测,发现L1ES对已注射卡介苗(BCG)或感染旋毛虫的小鼠,均能诱生肿瘤细胞毒因子(TCF),而正常对照鼠则不能发生,表明L1ES诱生TCF需要首先激活巨噬细胞(Mφ)这一基本条件。将L1ES置37℃、1h,56℃、30min或100℃、2min处理后,其诱生TCF的能力均明显高于对照组(P<0.05)。L1ES 相似文献
36.
Ko-Huang Lue Yung-Hsiang Lin Hai-Lun Sun Ko-Hsiu Lu Jie-Cheng Hsieh Ming-Chih Chou 《Pediatric allergy and immunology》2006,17(6):408-415
Immunotherapy through oral routes is thought to be a valuable therapeutic option for asthma. The clinical and immunologic effects of sublingual immunotherapy (SLIT) in children with asthma caused by mites were evaluated in a double-blind, placebo-controlled study for 6 months. Patients (aged 6-12 yr) with mild-to-moderate asthma, with single sensitization to mite allergen, received either SLIT or placebo with a standardized Dermatophagoides pteronyssinus (D.p.)/D. farinae (D.f.) 50/50 extract. The cumulative dose was around 41824 IR, equivalent to 1.7 mg of D.p. and 3.0 mg of D.f. allergen. Symptom and medication scores were assessed throughout the study. Serum total immunoglobulin (Ig)E, eosinophil count, eosinophil cationic protein, specific IgE, specific IgG4, and skin sensitivity were evaluated before starting the treatment and after the treatment period. Twenty patients completed the study. At the beginning of the treatment, no differences were observed between the groups for symptom and medication scores, skin sensitivity, or immunologic parameters. After 6 months of treatment, there was a significant difference in nighttime asthma symptom scores and specific IgG4 (p < 0.05) in the SLIT group compared with the placebo group. Daytime symptom and medication scores, total IgE, eosinophil count, forced expiratory volume in 1 s, and mean evening peak expiratory flow rate reached significant differences in the SLIT group during the treatment period (p < 0.05). No severe adverse effects were reported. Our results revealed that treatment for 6 months with SLIT is clinically effective in decreasing asthmatic symptoms and medication use in children with mild-to-moderate asthma because of mite sensitivity. The clinical usefulness of this form of immunotherapy and the mechanism underlying its immunologic effects deserve further studies. 相似文献
37.
38.
本文观察16例大肠癌病人的癌组织培养上清液对人T细胞的增殖,ZL—2产生的影响和病理Dukes分期的关系,发现癌组织的TDSF对T细胞的增殖有显著抑制作用,对ZL—2的产生抑制作用不明显,对T细胞增殖率在DukesA与B期,其值与对照有显著差异(0.01P<0.05).在C期有非常显著差异(P<0.01).说明大肠癌组织确实分泌有能造成宿主免疫功能下降的抑制物质.且免疫作用不是通过ZL—2环节发生. 相似文献
39.
目的:探讨着色干皮病(xeroderma pigmentosum,XP)伴发鳞状细胞癌(squamous cell careinoma,SCC)的临床病理特点。方法:采用组织病理学方法对1993年~2004年间收集的XP伴发SCC患者进行分析。结果:4例中,男性1例,女性3例。发病年龄最小1岁,最大4岁,平均2.5岁。并发肿瘤年龄,最小7岁,最大18岁,平均12.8岁。其中有明确近亲婚配者2例(50%)。4例患者临床症状及病理结果均典型。结论:XP为常染色体隐性遗传性皮肤病,是一种癌前病变,以早年并发恶性肿瘤为其特征,其中以鳞状细胞癌和基底细胞癌最为常见。与皮肤损害和紫外线损伤程度密切相关。 相似文献
40.
Patch clamp techniques were used to study whole cell ionic currents in Schwann cells (SC) from a tropical marine fish, the bicolor damselfish, Pomacentrus partitus. The bicolor damselfish is affected by a disease termed damselfish neurofibromatosis (DNF), being developed as an animal model of neurofibromatosis-type 1 (NF1) in humans. NF1 affects SC, fibroblasts, and perineurial cells. The sole depolarization-activated ionic current present in cultured SC from normal fish peripheral nerve and from neurofibromas of fish with induced or spontaneously occurring DNF was an inactivating K+ current (K current), with a strong dependence on the Nernst potential for K+. This K current activated at depolarizations to -40 mV and above and inactivated during a maintained test pulse (0.2-1 s), but inactivation was significantly greater in tumored SC. Both currents were inhibited by 4-aminopyridine (Kd ? 1 mM) and by dendrotoxin (15 μM) but were insensitive to extracellular tetraethyammonium (≤ 150 mM), indicating that the whole cell currents were similar pharmacologically. The currents could be distinguished on the basis of their sensitivity to depolarized holding potential, with normal cells less sensitive. Half-inactivation of the current was -32 mV in normal cells and -38 mV in tumored cells. Inactivation curves constructed from the average normalized current for many SC were significantly different in normal and tumored cells. When the depolarized holding potential was maintained between test depolarizations, greater voltage-dependent inactivation in tumored cells was apparent. Normal cells maintained an average of 36% of peak current at a holding voltage of ?40 mV, while in tumored cells this average was 12%, a significant difference. © 1994 Wiley-Liss, Inc. 相似文献