原发性甲状腺机能减退症患者血清甲状腺激素自身抗体放射免疫法测定

了解临床甲减病人血中甲状腺素自身抗体（T4Ab）及3，5，3'-三碘甲状腺原氨酸自身抗体（T3Ah）分布状况。方法：采用放射免疫法测定120例原发性甲减患者的T3Ab、T4Ab。结果：甲减患者T3Ab、T4Ab的测定值分别为9．42±4．43％及10．73±4．67％．较正常组升高非常显著（n=120．P＜0．01）；其T3Ab、T4Ab的阳性率分别为37．50％及40．83％。对甲减分组，伴TGAb和（或）TMAb升高组的T3Ab、T3Ab测定值及阳性率均较不升高组显著升高（P＜0．01）。结论：T3Ab、T4Ab在原发性甲减患者血中阳性率较高．其测定有助于甲减的诊断。     

Effect of GH and IGF-I treatment on reproduction,growth, and plasma hormone concentrations in domestic nutria (Myocastor coypus)

The role of GH and IGF-I in the control of reproduction, growth, and hormone secretion in domestic nutria was examined. In the first series of experiments, we studied the effects of single and multiple (daily for 20 days) injections of recombinant hGH (15 microg/animal) on plasma triiodothyronine (T3), thyroxine (T4), and progesterone (P) concentrations, as well as on the duration of pregnancy (time between start of mating and birth of pups), number of pups born, and body weight of adult females and their newborn pups. In the second series of experiments, the effects of single and multiple (daily for 28 days) injections of recombinant hIGF-I (1 microg/animal) on plasma IGF-I, IGFBP-3, T3, T4 concentrations, the duration of pregnancy, and number of offspring delivered were assessed. It was found that either single or multiple GH treatment resulted in significant increase in plasma T3, T4, but not P concentration. Furthermore, it significantly increased the body weight of adults and newborn pups. No influence of GH on the duration of pregnancy and the number of offspring was observed. IGF-I treatment caused an increase in plasma IGF-I concentration, a reduction in plasma IGFBP-3, T3, and T4 concentrations, and a shorter duration of pregnancy but did not alter the number of pups delivered. Our observations suggest that GH and IGF-I may be involved in the control of hormone secretion, growth, and reproduction in domestic nutria. Reproductive processes are controlled by IGF-I rather than by GH, whilst GH may be involved in the stimulation of prenatal and postnatal growth. The differential effects of these substances on thyroid hormones and reproductive parameters suggest that the actions of GH on these processes are probably not mediated by IGF-I.     

A study on role of triiodothyronine (T3) hormone on the improvement of articular cartilage surface architecture

The present study was aimed to investigate the effect of triiodothyronine (T3) on the improvement of articular cartilage surface architecture at in vitro level. The T3 hormone was applied to neo-tissues in the range of 50, 100, 150 and 200 ng/ml for 5 weeks. At the end of the treatment, biochemical and histological evaluation was carried out in the neo-tissues. T3 hormone application significantly increased the collagen production in neo-cartilage tissues. The properties of tensile and compressive were significantly increased compared to the controls. However, T3 hormone application also induced hypertrophy. At the higher dose concentration of T3 hormone application, tensile and compressive properties were tremendously increased 4.3 and 4.6 fold respectively. Taking all these data together, it suggested that the T3 hormone application could be a potential agent to increase the functional properties such tensile and compressive in neo-tissues.     

三碘甲状腺原氨酸对缺血再灌注损伤后未成熟心肌细胞中Bax、Bcl-2的影响

目的通过三碘甲状腺原氨酸对缺血再灌注损伤后未成熟心肌细胞中Bax、Bcl-2表达的影响,探讨其作用机制。方法培养原代乳鼠未成熟心肌细胞,通过缺氧复氧建立乳鼠心肌细胞缺血再灌注模型。于缺氧复氧前期给予三碘甲状腺原氨酸处理,通过聚合酶链反应仪检测三碘甲状腺原氨酸干预后心肌细胞的Bax mRNA和Bcl-2 mRNA表达情况。结果缺氧复氧前短期给予三碘甲状腺原氨酸可下调乳鼠未成熟心肌细胞Bax的表达,上调Bcl-2的表达,减少心肌细胞凋亡。结论三碘甲状腺原氨酸可以提高未成熟心肌抗缺血再灌注损伤的能力,而对心肌细胞凋亡这种保护作用与心肌细胞Bcl-2和Bax的表达相关。