应用蛋白质芯片技术筛选人舌鳞癌细胞株差异性炎症因子的初步研究

目的研究应用蛋白质芯片(抗体芯片)技术筛选、分析人舌鳞状细胞癌细胞株中差异性炎症因子的效果,为进一步探讨口腔癌与炎症的关系奠定基础。方法体外培养人舌鳞癌细胞株UM-1、CAL-27、Tca-8113和人正常口腔黏膜上皮细胞,消化后分别提取胞内、胞外蛋白,上芯片孵育,封闭,清洗。采用激光扫描仪扫描芯片,Axon GenePix Pro6.0软件提取芯片数据,对获得的80种炎症因子表达数据采用AAH-CYT-G5软件分析,上调因子信号值以大于200、比值大于2.0为纳入标准;下调因子信号值以大于200、比值小于0.66为纳入标准,分别对3种人舌鳞癌细胞株(UM-1、CAL-27、Tca-8113)与人正常口腔黏膜上皮细胞,以及高侵袭性细胞株(UM-1、CAL-27)与低侵袭性细胞株(Tca-8113)进行两两比较,筛选出差异性炎症因子。挑选3种显著差异性炎症因子,用酶联免疫吸附法再次检测其蛋白表达,所得结果用单因素方差分析进行统计学分析,进一步验证芯片法所获得的结果。结果根据纳入标准,从检测的80种因子中筛选出有表达的炎症因子9个,包括干扰素诱导蛋白10(inter-feron inducible protein10,IP-10)、巨噬细胞炎症蛋白-1β(macrophage inflammatory protein1β,MIP-1β)、巨噬细胞炎症蛋白-3α(macrophage inflammatory protein-3α,MIP-3α)、骨保护素蛋白(osteoprotegerin,OPG)、调节活化蛋白(regulated upon activation normal T-cell expressed and secreted,RANTES)、白细胞介素1β(interleukin1β,IL-1β)6种舌鳞癌细胞株中高表达的细胞因子,及单核细胞趋化因子4(monocyte chemoattractant protein4,MCP-4)、胰岛素样生长因子结合蛋白4(insulin-like growth factor binding protein-4,IGFBP-4)、转化生长因子-β3(transforminggrowth factor-β3,TGF-β3)3种舌鳞癌细胞株中低表达的细胞因子。鳞状细胞癌细胞和正常口腔鳞状上皮细胞之间比较,胞内IL-1β呈高表达,MCP-4呈低表达,胞外OPG呈高表达;高、低侵袭性细胞株之间比较,胞内RANTES呈高表达,IGFBP-4、TGF-β3呈低表达,胞外IP-10、MIP-1β、MIP-3α呈高表达。酶联免疫吸附法检测发现UM-1和CAL-27胞外IP-10、MIP-1β、MIP-3α的表达量和Tca-8113相比呈高表达,差异有统计学意义(P<0.05)。其结果与芯片筛选结果一致。结论蛋白质芯片技术能够较为准确地筛选出不同舌鳞癌细胞株之间差异性炎症因子,这些差异性炎症因子可能与肿瘤细胞的生物学行为有一定的联系。     

模拟失重及高+Gx对猴舌横纹肌细胞结构及HSP70表达的影响

目的探讨模拟失重及高+Gx对猴舌横纹肌组织结构和热休克蛋白70(heat shock protein 70,HSP70)表达的影响。方法 12只雄性猕猴随机分为4组,正常对照组(A)、30d模拟失重组(B),+13Gx/230s组(C)、30d模拟失重后超重组(D),其中D组根据过载峰值又分为+11Gx/270s(D1)、+13Gx/230s(D2)和+15Gx/200s(D3)。动物放血处死后取材,采用组织病理学和免疫组化的方法,观察猴舌横纹肌组织结构及HSP70的表达情况。结果 A组可见正常的舌横纹肌结构,其他组舌横纹肌细胞结构基本正常,偶见细胞间质稀疏,细胞排列紊乱以及横纹不清或消失。A组舌横纹肌细胞HSP70呈阴性表达,细胞核与细胞浆均无明显着色;B组、C组和D组舌横纹肌细胞胞核与胞浆均着色明显,呈阳性表达,较A组变化显著。HSP70免疫组化积分显示,D组比B组和C组变化显著(P<0.05),但D1、D2、D3无明显差别(P>0.05)。结论 30d模拟失重和高+13Gx/230s均可使猴舌横纹肌细胞产生应激反应,引起HSP70的表达增强,并可能伴有损伤。模拟失重与过载超重具有协同作用,两者可能加重了对舌横纹肌的影响。     

异氟醚对人舌癌细胞基质金属蛋白酶-2表达的影响

目的 评价异氟醚对人舌癌细胞(Tea8113细胞)基质金属蛋白酶-2表达的影响.方法 取对数生长期的Tea8113细胞,制成单细胞悬液,接种于96孔培养板(5×103/ml,200 μl/孔)、6孔培养板(1×105/ml,5 ml/孔)、培养皿(5×105/ml,5 ml/皿)和Transwell小室[上层小室加入细胞悬液200μl(细胞密度5×105/ml),下层小室加入10％血清培养基500 μl],常规培养待细胞完全贴壁后进行实验.采用随机数字表法,将细胞分为3组(n=30):对照组(C组)、2％异氟醚2h组(Ⅰ1组)、2％异氟醚4h组(Ⅰ2组).C组不给予异氟醚,Ⅰ1组和Ⅰ2组细胞培养液中加入2％异氟醚分别孵育细胞2或4h.采用MTT法检测细胞活力,流式细胞术检测细胞凋亡率;以细胞划痕实验及Transwell小室实验检测细胞水平迁移和侵袭能力;采用免疫细胞化学及RT-PCR检测细胞MMP-2及其mRNA表达水平.结果 与C组比较,Ⅰ1组、Ⅰ2组细胞活力和细胞水平迁移率升高,细胞凋亡率降低,MMP-2及其mRNA表达上调,Ⅰ2组透膜细胞数增多(P＜0.05).与Ⅰ1组比较,Ⅰ2组细胞活力和细胞水平迁移率升高,细胞凋亡率降低,透膜细胞数增多,MMP-2及其mRNA表达上调(P＜0.05).结论 异氟醚可能通过上调人舌癌Tca8113细胞的MMP-2表达来促进癌细胞的迁移侵袭能力.     

Screening for Smoking in a General Hospital: Scale Validation,Indicators of Prevalence,and Comorbidity

PURPOSE: The study aims to conduct a psychometric study of the Fagerström Test for Nicotine Dependence (FTND) and to assess the prevalence, sociodemographic profile, and psychiatric comorbidities of smokers in a general hospital. DESIGN AND METHODS: Two hundred sixty‐four inpatients were elegible for inclusion in the study and assessed with Structured Clinical Interview for the DSM‐IV (Module E), Patient Health Questionnaire, Fast Alcohol Screening Test, and FTND. FINDINGS: The cut‐off note two of the FTND is the most adequate to identify tobacco abuse/dependence (sensibility: 0.59–0.76). The reliability was estimated to be 0.68–0.96. A 44% rate of tobacco abuser/dependent patients was detected, with a 28% rate of psychiatric comorbidity. PRACTICE IMPLICATIONS: Detection of the smoking habits at the time of hospitalization can be opportune, favoring the planning and the beginning of treatment.     

Évaluation gnoso-praxique linguale des enfants de 4 ans et demi à 9 ans et demi

The study of lingual gnosis and praxis skills of children aged from 4.5 years old to 9.5 years old has been realized on normal children and on children under speech therapy for speech and language disorder. The comparison of normal children between our research and the one of Tardieu shows that today's children aged between 6.5 and 9.5 years have lower gnostic skills than in 1973. We observe a lingual gnostic deficit for children of all ages receiving speech therapy, the gap tending to fade as they get older. It seems appropriate to integrate a speech-language assessment test to better understand the gnostic deficit of children with a verbal orality and/or eating disorder. Such information would help to define the therapeutic approach of the speech therapy and thus promote the education gnoso-oral praxis early.     

Effect of polyglycolic acid sheets with fibrin glue (MCFP technique) on the healing of wounds after partial resection of the border of the tongue in rabbits: a preliminary study

The aim of this study was to examine the effectiveness of covering wounds to the tongue with a polyglycolic acid (PGA) sheet and fibrin glue. Eighteen mature male Japanese white rabbits had a unilateral glossectomy involving an area 10mm×10mm×2mm. After glossectomy the tongues were covered with PGA sheets 8mm×8mm in size and fibrin glue (mucosal defect covered with fibrin glue and polyglycolic acid sheet=MCFP) 1 week after the operation (n=3), after 2 weeks (n=3), and after 4 weeks (n=3). In control groups, after 1, 2, and 4 weeks (n=3 in each group), the partially resected tongues were closed with absorbable sutures (polyglactin 910). One week (experimental and control groups 1), 2 weeks (experimental and control groups 2) and 4 weeks (experimental and control groups 3) after operation the tongues were harvested and stained for microscopic examination. Histological examination showed that the covered wound surface had not epithelialised and the basal layer had yet to form in experimental group 1, but had formed in experimental group 2. However, in control group 1, epithelialisation of the sutured wound had begun. Immunohistochemical examination showed that, in experimental group 1, the non-uniform epithelial layer of the covered wound surface expressed cytokeratin AE1/AE3, and the epithelial and connective tissue layers stained strongly for FGF-2. Similar results were obtained in experimental group 2, whereas in experimental group 3, FGF-2 was expressed only in the connective tissue layer, and epithelialisation was complete. However, in control group 1, AE1/AE3 was expressed in the epithelial layer, and FGF was expressed in the connective tissue layer beneath the basal layer. In control groups 2 and 3, AE1/AE3 and FGF-2 were expressed in patterns similar to those in experimental groups 2 and 3. We suggest that this method is useful and the operation is simple. However, further testing of the method is needed and it should be widely used clinically before it is recommended.     

Controlling posture using a plantar pressure-based,tongue-placed tactile biofeedback system

The present paper introduces an original biofeedback system for improving human balance control, whose underlying principle consists in providing additional sensory information related to foot sole pressure distribution to the user through a tongue-placed tactile output device. To assess the effect of this biofeedback system on postural control during quiet standing, ten young healthy adults were asked to stand as immobile as possible with their eyes closed in two conditions of No-biofeedback and Biofeedback. Centre of foot pressure (CoP) displacements were recorded using a force platform. Results showed reduced CoP displacements in the Biofeedback relative to the No-biofeedback condition. The present findings evidenced the ability of the central nervous system to efficiently integrate an artificial plantar-based, tongue-placed tactile biofeedback for controlling control posture during quiet standing.     

Inter-individual variability in sensory weighting of a plantar pressure-based, tongue-placed tactile biofeedback for controlling posture

The purpose of the present experiment was to investigate whether the sensory weighting of a plantar pressure-based, tongue-placed tactile biofeedback for controlling posture could be subject to inter-individual variability. To achieve this goal, 60 young healthy adults were asked to stand as immobile as possible with their eyes closed in two conditions of No-biofeedback and Biofeedback. Centre of foot pressure (CoP) displacements were recorded using a force platform. Overall, results showed reduced CoP displacements in the Biofeedback relative to the No-biofeedback condition, evidencing the ability of the central nervous system to efficiently integrate an artificial plantar-based, tongue-placed tactile biofeedback for controlling posture during quiet standing. Results further showed a significant positive correlation between the CoP displacements measured in the No-biofeedback condition and the decrease in the CoP displacements induced by the use of the biofeedback. In other words, the degree of postural stabilization appeared to depend on each subject's balance control capabilities, the biofeedback yielding a greater stabilizing effect in subjects exhibiting the largest CoP displacements when standing in the No-biofeedback condition. On the whole, by evidencing a significant inter-individual variability in sensory weighting of an additional tactile information related to foot sole pressure distribution for controlling posture, the present findings underscore the need and the necessity to address the issue of inter-individual variability in the field of neuroscience.     

How a plantar pressure-based,tongue-placed tactile biofeedback modifies postural control mechanisms during quiet standing

The purpose of the present study was to determine the effects of a plantar pressure-based, tongue-placed tactile biofeedback on postural control mechanisms during quiet standing. To this aim, 16 young healthy adults were asked to stand as immobile as possible with their eyes closed in two conditions of No-biofeedback and Biofeedback. Centre of foot pressure (CoP) displacements, recorded using a force platform, were used to compute the horizontal displacements of the vertical projection of the centre of gravity (CoG _v ) and those of the difference between the CoP and the vertical projection of the CoG (CoP-CoG _v ). Analysis of the CoP-CoG _v displacements showed larger root mean square (RMS) and mean power frequencies (MPF) in the Biofeedback than in the No-biofeedback condition. Stabilogram-diffusion analysis further showed a concomitant increased spatial and reduced temporal transition point co-ordinates at which the corrective processes were initiated and an increased persistent behaviour of the CoP-CoG _v displacements over the short-term region. Analysis of the CoG _v displacements showed decreased RMS and increased MPF in the Biofeedback relative to the No-biofeedback condition. Stabilogram-diffusion analysis further indicated that these effects mainly stem from reduced spatio-temporal transition point co-ordinates at which the corrective process involving CoG _v displacements is initiated and an increased anti-persistent behaviour of the CoG _v displacements over the long-term region. Altogether, the present findings suggest that the main way the plantar pressure-based, tongue-placed tactile biofeedback improves postural control during quiet standing is via both a reduction of the correction thresholds and an increased efficiency of the corrective mechanism involving the CoG _v displacements.     

孕期保健及营养指导对妊娠期糖尿病孕妇母婴结局及行为习惯的影响

目的探讨孕期系统性、针对性保健及营养指导对妊娠期糖尿病(GDM)孕妇母婴结局及行为习惯的影响,为GDM的治疗提供理论依据。方法选取2014年1月-2016年12月成都市金牛区妇幼保健院妇产科收治的320例GDM孕妇为研究对象,采用随机数表法分为对照组和观察组,每组各160例,对照组给予常规孕期保健,观察组在对照组的基础上给予孕期系统性、针对性保健及营养指导。观察两组孕妇干预前后空腹血糖、餐后2h血糖及糖化血红蛋白水平,对比两组孕妇妊娠结局和围生儿结局及两组科学饮食、适当运动、自测血糖、定时产检、讲究卫生等行为习惯的差异。结果干预前,两组空腹血糖、餐后2h血糖及糖化血红蛋白水平比较,差异无统计学意义(P>0.05);干预后,两组空腹血糖、餐后2h血糖及糖化血红蛋白水平均明显降低,且观察组低于对照组,两组相比差异有统计学意义(P<0.05)。两组孕妇剖宫产、妊娠期高血压疾病、羊水过多、感染、胎膜早破、产后出血发生率比较,差异有统计学意义(P<0.05)。两组早产、新生儿低血糖、巨大儿、胎儿窘迫、新生儿窒息、围生儿死亡发生率比较,差异有统计学意义(P<0.05)。观察组孕妇合理膳食、适当运动、卫生习惯、定期产检、自测血糖等行为习惯评分均高于对照组,两组比较,差异有统计学意义(P<0.05)。结论对GDM孕妇实施孕期系统的、针对性保健及营养指导效果显著,可有效控制血糖,改善母婴结局及孕妇不良行为习惯。