尿微量蛋白尿酶对高血压肾损害早期诊断的临床意义

通过检测尿中微量白蛋白及尿中的多项酶,能预测高血压病对肾脏的早期损害。如何使用简单无创的方法在肾损害的最早期做出诊断成为众所关注问题和焦点。目前,尿微量白蛋白尿、α1微球蛋白、β2微球蛋白、视黄醇结合蛋白、N-乙酰-β-D氨基葡萄糖苷酶、尿溶菌酶测定已被证实可反映损害的部位和程度,并可作为判断疗效和监测预后的指标。     

2种方法检测梅毒螺旋体结果分析

目的对2种方法检测梅毒螺旋体的结果进行分析。方法对47555人次无偿献血者分别用甲苯胺红不加热血清试验(TRUST)、酶联免疫吸附试验(ELISA)两种方法做梅毒螺旋体血清学检测,对初检阳性的标本进行梅毒螺旋体明胶颗粒凝集试验(TPPA)加以确认。结果47555个样本中,TRUST法阳性率0.39%,ELISA法阳性率0.8%。TPPA法确认的340份标本中,TRUST法检出153份,占45%(153/340),ELISA法检出340份,占100%(340/340)。结论ELISA法检测敏感度高于TRUST法,但ELISA法存在假阳性,只有两种方法联合使用,才能保证血液安全。     

双酚A对雄性小鼠生殖毒性的影响

目的观察环境雌激素双酚A（BPA）对雄性小鼠生殖功能的损害作用。方法采用小鼠腹腔注射双酚A，染毒成年小鼠5d，饲养30d。观察小鼠精子畸形率、测定血清中一氧化氮（NO）的含量、一氧化氮合酶（NOS）的活性。以雌二醇（E2）作为阳性对照物。结果BPA染毒组小鼠的精子畸形率较对照组高，睾丸和附睾的脏器系数均低于对照组，血清NO含量、NOS活性均高于对照组。结论一定剂量的BPA对小鼠的精子有致畸作用，并可使血清NO含量、NOS活性增高。     

IL-13Rα2、VEGF和PTEN在人脑胶质瘤中的研究进展

胶质瘤是中枢神经系统发生率最高的恶性肿瘤,其治疗仍然是一大难题。近年来,对脑胶质瘤发病的相关分子机制和凋亡机制已进行了广泛而深入的研究,为进一步探讨胶质瘤的分子病因、分子分型、恶性进展、基因治疗及预后评估奠定了基础,尤其是对白细胞介素13受体α2(IL-13Rα2)、血管内皮生长因子(VEGF)及人10号染色体上缺失的磷酸酶和张力蛋白类似物(PTEN)的深入研究。本文总结了IL-13Rα2、VEGF和PTEN的研究进展及其临床意义。     

知母皂苷元对HEK293sw细胞APP和BACE1的影响

目的 探讨知母活性成分知母皂苷元(ZMS)对HEK293sw细胞淀粉样前体蛋白(APP)和β-位点APP切割酶1(BACE1)的影响.方法 常规培养HEK293sw细胞,加入不同浓度ZMS预处理24 h后,换为无血清培养,继续处理48 h后观察ZMS的作用.Western blotting和RT-PCR技术测定APP和主要β-分泌酶BACE1的表达;荧光分析法测定BACE1活力.结果有效终浓度1 μmol/L和10 μmol/L的ZMS对BACE1的mRNA和蛋白表达均有显著降低作用,但对APP的mRNA和蛋白表达无明显影响.荧光分析结果显示,10 μmol/L ZMS能显著降低BACE1活力.结论 ZMS显著降低HEK293sw细胞主要β-分泌酶BACE1的表达及活力,而对APP的表达无明显影响.     

Peroxisome proliferator-activated receptor α agonist attenuates oxidized-low density lipoprotein induced immune maturation of human monocyte-derived dendritic cells

Accumulating evidence suggests that the Thl immune .response induced by various antigens such as oxidized low density lipoprotein （ox-LDL） and heat shock proteins （HSPs） play a key role in the process of atherosclerosis.1Dendritic cells （DCs） are the most potent antigen-presenting cells （APCs） in the body with the unique ability to initiate a primary immune response to certain antigens by the activation of ＂naive＂ T cells.2 The maturation of DC with the upregulation of costimulatory molecules such as CD83, CD40, CD86, and major histocompatibility complex （MHC） class molecules such as human leukocyte antigen （HLA）-DR, is required for DC to activate T cells. Pathologic studies have shown that immature DCs are present in normal arterial while abundant mature DCs clustered with T cells could be visualized in atherogenic vessels suggesting that DC 3 maturation is linked to the progression of atherosclerosls. Peroxisome proliferator-activated receptors （PPARs） a, one member of the family of PPARs, was found to have favorable effects on slowing the progression of atherosclerosis and reducing the risk of coronary heart disease in high-risk patients independent from their metabolism effects.4＇5 Furthermore, PPAR-α is also expressed on monocytes and monocyte-derived DCs.6 The effects of PPAR-α on DCs maturation and immune function remain unknown now, we therefore observed the effects of fenofibrate, a PPAR-α agonist, on the maturation and immune function of oxidized LDL-treated DCs in this study.     

Serum hepatic enzyme manifestations in patients with severe acute respiratory syndrome： Retrospective analysis

AIM: To evaluate the hepatic function in patients with severe acute respiratory syndrome (SARS) and possible causes of hepatic disorder in these patients. METHODS: One hundred and eighty-two patients with SARS were employed in a retrospective study that investigated hepatic dysfunction. Liver alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactic dehydrogenase (LDH) were analyzed in these patients. Patients with different hospital treatments were further investigated. RESULTS: Of the 182 patients, 128(70.3%) had abnormal ALT activity, 57(31.3%) had abnormal AST activity and 87(47.8%) had abnormal LDH activity. The peak of elevated hepatic enzyme activities occurred between the sixth day and the tenth day after the first day of reported fever. Of the 182 patients, 160(87.9%) had been treated with antibiotics, 137(75.2%) with Ribavirin, and 115(63.2%) with methylpredisolone. There was no statistically significant correlation between the duration of Ribavirin treatment and hepatic dysfunction. CONCLUSION: Abnormal liver functions were common in patients with SARS and could be associated with virus replication in the liver.     

降酶汤治疗转氨酶异常32例临床观察

目的:观察以"复合物"假说为指导组方的降酶汤治疗转氨酶异常的疗效。方法:将60例难治性转氨酶异常患者随机分为治疗组(32例)和对照组(28例),对照组采用甘利欣胶囊治疗,治疗组运用甘利欣合降酶汤治疗,观察治疗前后的临床疗效、ALT及HBeAg的变化。结果:治疗组的临床疗效及ALT优于对照组(P<0.01),HBeAg优于对照组(P<0.05),具有显著差异。结论:降酶汤对转氨酶和HBeAg的复常有一定的疗效。     

三种止血药治疗胸部闭合性损伤的最小成本分析

目的：评价3种止血药在胸部闭合性损伤治疗中的成本-效果比。方法：对本院急诊科收治的121例患者用成本-效果分析方法进行评价。结果：血凝酶组治疗效果显著优于另外两组，且成本效果比最佳。结论：血凝酶是治疗胸部闭合性损伤的最佳方案。