益气活血化痰法对血管性痴呆大鼠脑组织中内皮素和降钙素相关基因肽的影响

目的 :通过对VD大鼠脑组织ET 1和CGRP含量的测定 ,探讨其在VD形成过程中的发病机制和中药干预机制。方法 :采用放射免疫分析法测定脑组织ET 1和CGRP浓度。结果 :1 模型组、正常组与假手术组比较ET 1含量升高 ,CGRP含量降低 ,均有非常显著性差异 (P <0 0 1) ;2 大剂量组ET 1含量变化较治疗前明显降低 ,CGRP含量较治疗前明显升高 ,均有非常显著性差异 (P <0 0 1)。讨论 :VD在形成过程中ET 1含量增高 ,CGRP含量降低 ,失去二者的平衡 ,中药能有效调节二者的平衡 ,是防治VD的可能机制之一。     

山东新华PS-100低温等离子灭菌器故障维修

低温等离子灭菌器是医院消毒供应中心一种较为常见的医疗设备,其工作原理主要是利用过氧化氢在极度真空的灭菌舱环境中汽化后均匀扩散到整个空间,再由高频电压产生高频电场使得过氧化氢气体形成等离子体体系,多个因子间共同起到杀菌的效果。灭菌处理的全部过程均是在干燥的低温情况下进行,所以基本不会对医疗器械产生破坏作用,且不会残留任何毒性,因此其在金属与非金属的灭菌处理中均可使用。本院使用山东新华PS-100低温等离子灭菌器,为医疗器械灭菌消毒工作提供了诸多便利,但使用期间曾出现真空期超时、提纯器温度太低、扩散期压力太低、线路等故障,现就其检修过程进行分析,以供参考。     

王檀教授从"火"论治哮病

王檀教授认为,哮病具有迁延难治且反复发作的特点,在北方严寒之地其发病率更为突出.引起哮病发作的原因错综复杂,其病因无外乎外感及内因.提出"哮病主于火"及"五脏六腑皆令人哮"为哮病的病机关键.从脏腑相干理论出发提出哮病因火、热而哮,因痰而作.治疗时,当辨清五脏六腑的寒热虚实,调整脏腑功能,尤其脾胃的功能的健运.从火、热论治哮病,发则治其标,缓则治其本.     

谭新华工作室无子(男性不育症)中医诊疗方案

夫妇婚后同居1年以上,有正常规律的性生活,未采用任何避孕措施,由于男方原因,造成女方不能受孕,或能使女方受孕但不能成功分娩者称为男性不育症.男性不育症不是一个独立的疾病,是由一种或多种疾病因素、理化因素及不良生活方式作用于生殖众多环节后导致的一种病证.本症临床上可分为绝对性不育、相对性不育、原发性不育、继发性不育4种类型.本病属于中医学“无子”、“无嗣”、“男子艰嗣”范畴.     

Tanshinone IIA inhibits cell growth by suppressing SIX1-induced aerobic glycolysis in non-small cell lung cancer cells

Aerobic glycolysis plays a key role in cancer cell metabolism and contributes to tumorigenesis, including that of non-small cell lung cancer (NSCLC). Tanshinone IIA (Tan IIA), an active compound of Salvia miltiorrhiza, exhibits antitumor properties. Multiple mechanisms are involved in the antitumor action of Tan IIA in lung cancer, such as inhibiting cell growth, promoting cell apoptosis and influencing cellular metabolism. However, the effects of Tan IIA on NSCLC cells and its mechanisms of action remain unclear. The present study shows Tan IIA dose-dependently attenuated the growth of NSCLC cells and in vitro in a dose-dependent manner. Moreover, Tan IIA markedly decreased the ATP level, glucose uptake and lactate production in the NSCLC cells in vitro. Tan IIA also inhibited tumor growth in a xenograft model in vivo. Mechanically, Tan IIA treatment decreased sine oculis homeobox homolog 1 (SIX1) mRNA and protein levels, thus leading to the downregulation of pyruvate kinase isozyme M2, hexokinase 2 and lactate dehydrogenase A (LDHA) expression in A549 cells. SIX1 knockdown with small interfering-RNA inhibited glycolysis in NSCLC cells, suggesting that SIX1 plays a role in the antitumor effect of Tan IIA on NSCLC cells. More importantly, it was demonstrated that SIX1 expression was stimulated in patients with NSCLC and was positively correlated with the LDH serum level. Finally, SIX1 low expression levels predicted the poor prognosis of patients with NSCLC. In conclusion, the present study showed that Tan IIA functioned as an anti-glycolysis agent in NSCLC cells by downregulating SIX1 expression and inhibiting cell proliferation.     

牛磺酸拮抗溶血磷脂酸诱导的大鼠血管平滑肌细胞增殖作用

目的　观察牛磺酸 (Tau)对溶血磷脂酸 (LPA)诱导的大鼠血管平滑肌细胞 (VSMC)增殖作用的影响。方法　在培养的大鼠血管平滑肌细胞上 ,测定3 H 胸腺嘧啶核苷(3 H TdR)参入和丝裂素活化蛋白激酶 (MAPK)活性 ;硫代巴比妥酸盐 (TBA)法测脂质过氧化终产物丙二醛 (MDA)含量。结果　LPA (1× 10 -9～ 1× 10 -7mol·L-1)呈浓度依赖性增加VSMC 3 H TdR参入 ,5、10和 2 0mmol·L-1Tau及MAPK抑制剂PD0 980 59预孵育后分别使LPA (1× 10 -7mol·L-1)诱导的3 H TdR参入较单独LPA组减少 15 %、34%、4 8%和 5 4 % (P <0 0 5或P <0 0 1)。LPA增加VSMCMAPK活性 ,5、10和 2 0mmol·L-1Tau预孵育降低LPA(1× 10 -7mol·L-1)刺激的MAPK活性 ,较单纯LPA孵育低14 %、35 %和 4 4 % (P <0 0 5或P <0 0 1)。随着加入LPA的浓度增加 ,细胞MDA含量增加。与 1× 10 -7mol·L-1LPA组比较 ,5、10和 2 0mmol·L-1Tau预处理的细胞较LPA单独孵育组细胞MDA含量分别低 18%、2 7%、4 5 % (P<0 0 5或P <0 0 1) ,LPA诱导细胞MDA生成的作用不受PD0 980 59预孵育的影响 (P >0 0 5 )。结论　LPA呈浓度依赖性促进VSMC增殖和脂质过氧化 ,前者与其细胞内信号传导MAPK途径有关 ,Tau可有效拮抗LPA诱导的VSMC增殖、MAPK激活和脂质过氧化损伤 ,Tau     

补脑振痿胶囊治疗缺血性中风后遗症的临床研究

补脑振瘘胶囊根据张锡纯所创汤剂,研制出的一种新剂型。用于治疗缺血性中风后遗症30例,并与偏瘫复原丸治疗的20例对比研究。结果显示：补脑振瘘胶囊组临床痊愈、显效、有效及有效率均高于偏瘫复原丸组,且治疗效果随疗程的增加而递增,对病程较长的患者疗效明显优于偏瘫原复丸组,肌力改善情况明显优于偏瘫复原丸组。     

Australian adolescents' sun protection behavior: who are we kidding?

PURPOSE: To examine the influence of tan preference and skin type on sun protection behaviors of Australian adolescents. METHODS: The Australian Secondary School Alcohol and Drug Questionnaires were conducted in 1993, 1996, 1999 and 2002 among randomly selected students aged 12--17. Schools were randomly selected from each education sector in each state. The self-administered questionnaire contained questions about tan preferences, skin type and usual SunSmart behavior (use of sunscreen, hats and covering clothing). RESULTS: The routine use of SunSmart behavior was low in all survey years. There was a significant decrease over time in the proportion of students who practiced SunSmart behavior, with prevalence rates lower in 2002 than in any other survey year (males: p<0.01 and females: p<0.01). As desire for a tan increased, routine practice of SunSmart behaviors decreased. Across the four survey periods, male (p<0.01) and female (p<0.01) students who preferred no tan were significantly more likely to practice SunSmart behavior than students who preferred any sort of tan. Across the four survey years, male (p<0.01) and female (p<0.01) students with skin that 'just burns' were most likely to routinely practice SunSmart behavior. CONCLUSIONS: Sun protection practices among adolescents have continued to decline significantly over time. Future educational programs require an innovative approach to modify adolescent behaviors in relation to sun exposure and sun protection.     

Recurrent pyogenic cholangitis – an independent poor prognostic indicator for resectable intrahepatic cholangiocarcinoma: A propensity score matched analysis

Background

Recurrent pyogenic cholangitis (RPC) is a known risk factor for intrahepatic cholangiocarcinoma (ICC), whether it represents a poor prognostic factor remains controversial. The aim of this study was to investigate the post-hepatectomy oncological outcomes of patients with ICC and coexisting RPC.