Modulation of genotoxicity and DNA repair by plant monoterpenes camphor, eucalyptol and thujone in Escherichia coli and mammalian cells

The aim of this work was to examine the antigenotoxic potential of plant monoterpenes: camphor, eucalyptol and thujone in prokaryotic and eukaryotic cells and to elucidate their effect on DNA repair. We compared the effect of monoterpenes on spontaneous, UV- and 4NQO-induced mutagenesis in Escherichia coli K12 repair proficient, and MMR and NER deficient strains. Positive controls tannic acid and vanillin were included in bacterial tests. We also examined protective effect of monoterpenes against 4NQO-induced genotoxicity in Vero cell line by alkaline comet assay. The results obtained in repair proficient strain indicated antimutagenic potential of monoterpenes against UV- and 4NQO-induced mutagenesis, which was diminished with NER deficiency. Camphor and eucalyptol maintained UV-induced SOS response longer than in controls, while thujone decreased SOS response and reduced general protein synthesis and the growth rate. The three monoterpenes increased spontaneous and UV-induced recombination in recA730 and camphor additionally in recA⁺ cells. Incubation of 4NQO-pretreated Vero cells with monoterpenes resulted in significant reduction of tail moment. However, higher concentrations of monoterpenes induced DNA strand breaks. Obtained results indicate that by making a small amount of DNA lesions camphor, eucalyptol and thujone can stimulate error-free DNA repair processes and act as bioantimutagens.     

论无痰不作眩

本文重点从《丹溪心法》中“无痰不作眩”观点出发,论述风、气、郁、瘀、湿、热相关因素均可致痰或与痰相结而成眩晕之六大证型,并分析其病因病机、治则、方药,为临床中痰证所致眩晕提供辨证治疗思路。     

痰热清注射液联合抗生素治疗老年下呼吸道感染临床观察

目的比较痰热清联合抗生素治疗老年下呼吸道感染的临床疗效。方法124例被随机分成两组,治疗老年下呼吸道感染。结果老年下呼吸道感染耐药情况比较严重,痰热清合并抗生素治疗能缩短疗程。结论痰热清是治疗老年下呼吸道感染的安全有效的药物。     

参南瘫速康胶囊治疗缺血性中风失语临床观察

目的 :观察参南瘫速康胶囊治疗缺血性中风的临床疗效。方法 :观察组给予参南瘫速康胶囊 (西洋参、胆南星、白附子、红花、水蛭、金钱白花蛇、全蝎、蜈蚣、天麻、石菖蒲 ) 5 3例 ,对照组采用针刺疗法治疗。结果 :观察组 5 3例中 ,临床治愈 18例 ,有效率 88.6 8% ;对照组 34例中 ,临床治愈 8例 ,有效率 6 7.6 5 % ,两组疗效比较有显著差异 (P <0 .0 1)。提示 :参南瘫速康胶囊对缺血性中风失语有补气培元、祛风化痰、活血化瘀、开窍益神之效。     

Epidemiology of preterm birth in China in 2015 and 2016: a nationwide survey

Background

Preterm birth is the most common cause of perinatal morbidity and mortality. China has one of the highest numbers of preterm births worldwide each year. However, the epidemiology of preterm birth in China remains unclear.

A digitally guided dual technique for both gingival and bone resection during crown lengthening surgery

This paper presents a digitally guided dual technique that provides references for gingival and bone resection during crown lengthening surgery. The architecture of the teeth, gingiva, and alveolar bone is scanned and registered to design dual guides consisting of a gingivectomy guide and an alveolectomy guide that are used in periodontal surgery for esthetic rehabilitation.     

芪菖治瘫口服液对脑缺血再灌注损伤大鼠脑组织的实验指标的研究

目的：观察芪菖治瘫口服液对脑缺血及再灌注损伤大鼠脑组织时，氧自由基有关参数的变化及一氧化氮（NO）、一氧化氮合成酶（NOS）、超氧化物酶（SOD）及过氧化脂质的代谢产物丙二醛（MDA）、微量元素的变化。方法：36只健康大鼠分为6组：模型组、实验/治疗组、包括手术对照组，实验药物高低剂量组及阳性药物对照组。结果：芪菖治瘫口服液可明显减少脑缺血时丙二醛、锌的含量，NO、NOS、SOD、Cu^2 含量不同程度的升高。芪菖治瘫口服液高剂量组作用强于低剂量组，与阳性药物相比，此方剂优于其他方剂。结论：芪菖治瘫口服液有明显减轻脑缺血时，自由基对血液屏障与脑组织的损害作用。此研究为临床和基础研究提供实验依据。     

导痰汤加减临床应用举隅

导痰汤临床应用病机为痰邪,审证求因,谨守病机,临证以导痰汤加减脑供血不足、脂肪肝、帕金森病疗效满意。     

痰热清注射液治疗小儿颈部急性淋巴结炎疗效观察

目的:观察痰热清注射液治疗儿童颈部急性淋巴结炎的临床疗效。方法:46例小儿颈部急性淋巴结炎患儿随机分为治疗组与对照组各23例,治疗组23例采用痰热清注射液加常规抗生素治疗,对照组23例采用抗生素加病毒唑静脉滴注治疗。均7d为1个疗程。结果:治疗组治愈20例,有效2例,无效1例,总有效率96.65%;对照组治愈16例,有效4例,无效3例,总有效率86.96%;两组淋巴结消退时间比较,治疗组明显优于对照组。结论:痰热情注射液有较好的治疗儿童颈部急性淋巴结炎作用,可明显缩短疗程,有临床推广使用的价值。