谈勇辨治恶阻临床经验

介绍谈勇教授治疗恶阻经验。认为主证为肝胃不和,可兼脾胃虚弱、痰湿阻滞;治疗时在相应方药基础上辅以中药熏蒸,效果良好。并附验案1则。     

百岁女医谈允贤传奇医传——从《女医杂言》细论前明妇科、性医学的进步

2015年中国女医屠呦呦获得诺贝尔生理学及医学奖后,中医借此大善,于今岁再祭前明女医谈允贤氏。岁首电视剧《女医·明妃传》热映,故事情节虽跌宕,然非历史真实之谈允贤也。1999年中国中医研究院医史文献研究所郑金生撰文《明代女医谈允贤及其医案女医杂言》,第一次向世间介绍了这位著名的明朝女医生。此前该人医事且并不为人知,究为何?《女医杂言》在前明出版两次,今仅以后版(万历年版)存世,经初考似曾另著别"医案"(或医著)存世,而今仍无善本见存,内中缘由深矣。今经较详细考证有关史料,发现女医谈允贤或其家族成员,似于500年前曾卷进一桩被《明史》记为"壬寅宫变"的冤案当中。至于这宗晚明时期的杀医要案,久不查者甚矣,今当昭雪于世界,以彰显为医者之正气!     

慢性酒精中毒对大鼠学习记忆功能和脑组织内CaN和Tau-Thr231的影响

建立慢性酒精中毒致大鼠学习记忆障碍模型,通过免疫组织化学法检测大鼠大脑皮层、海马和丘脑内钙神经素(calci-neurin,CaN)和Tau蛋白Thr231位点(Tau-Thr231)的分布与表达,探讨其在慢性酒精中毒致大鼠学习记忆障碍发病机理中的作用。成年雄性SD大鼠随机平均分为对照组和染毒组。对照组大鼠以生理盐水灌胃,染毒组则以55%酒精灌胃。运用Morris水迷宫检测大鼠学习记忆功能损伤情况,免疫组织化学法检测大脑皮层、海马和丘脑CaN和Tau-Thr231分布及表达,并测定CaN和Tau-Thr231免疫阳性细胞数。慢性酒精中毒后大鼠学习记忆功能有不同程度损伤。染毒组大脑皮层和海马CaN表达较对照组上调(P<0.05);染毒组大脑皮层和海马Tau-Thr231表达较对照组下调(P<0.05)。丘脑未见CaN表达,对照组丘脑可见Tau-Thr231免疫阳性细胞分布,染毒组Tau-Thr231免疫阳性细胞数量逐渐减少,提示慢性酒精中毒致大鼠学习记忆功能损伤可能与大鼠大脑皮层、海马和丘脑内CaN和Tau-Thr231的表达变化相关。     

The protective effects of nucleotides on the histamine hypersensitivity of Bordetella pertussis-treated mice

The effects of exogenous nucleotides on histamine hypersensitivity induced by Bordetella pertussis in mice were investigated. Female mice (HLA-SW/ICR), 26 to 28 Gm., were injected with one milligram of histamine intraperitoneally 4 days after the intravenous administration of B. pertussis. At different time intervals before histamine challenge, various doses (0.5 to 16 μmoles in saline) of nucleotides were administered intravenously. Control animals, likewise sensitized with B. pertussis, were given saline instead of nucleotides. Each group consisted of 10 mice, and mortality of control groups averaged approximately 70 per cent. Ribonucleotides of adenosine, AMP, and ADP showed clear, dose-response protection against histamine death of pertussis-treated mice, and the protective effect was highest at 90 minute intervals between nucleotide and histamine. cyAMP and DBcyAMP showed partial protection (average of 20 per cent mortality instead of 70 per cent) at very narrow dose ranges (cyAMP, 3 to 9 and DBcyAMP, 4 to 6 μmoles). Lower and higher doses gave equivocal or no protection. ATP (1 to 10 μmoles) was protective when it was given 10 minutes before histamine, and simultaneous or earlier administration reduced this effect. Deoxyribonucleotides of adenosine, AMP and ADP, did not show the protective effects at the same doses as corresponding ribonucleotides did. GMP and IMP were similar in potency to AMP in protection, but cyGMP, cyIMP, and pyrimidine nucleotides were not protective. Similar protective effect of nucleotides was observed in passive systemic anaphylaxis. Four days after pertussis treatment, mice were passively sensitized by intraperitoneal injection of mouse anti-EA and challenged with EA 5 hours later. The nucleotides were given 90 minutes before antigen challenge. The nucleotides that protected mice from histamine death also protected them from anaphylaxis. The possibility that exogenous nucleotides protect mice from histamine hypersensitivity through increase or stabilization of intracellular cyAMP levels, despite impairment of adenyl cyclase in pertussis-treated mice, is discussed.     

丹参酮ⅡA对肠缺血再灌注大鼠肺损伤的保护作用

目的：研究丹参酮ⅡA（tanshinone ⅡA）对肠缺血再灌注的大鼠肺损伤的保护作用及其可能的作用机制。方法：采用肠系膜上动脉夹闭 1 h-灌注 2 h 的方式复制肠缺血再灌注（ischemia reperfusion,IR）大鼠损伤的模型;将 SD 大鼠随机分为假手术组、模型组及丹参酮ⅡA （5,10,20 mg/kg ）组,各组于缺血前 20 min 舌下静脉给药,测定再灌注后大鼠血清及肺组织中 SOD 活力、MDA 和 NO 水平,并在光镜下观察肺组织形态学的变化。结果：模型组与假手术组相比,前者大鼠血清及肺组织中的 SOD 活力降低,MDA 和 NO 水平升高,经镜检发现大鼠肺脏组织中有明显的组织形态学的损伤;丹参酮ⅡA 组与模型组相比,前者呈剂量依赖性地增强大鼠 SOD 活力,降低 MDA 及 NO 水平,减轻大鼠肺组织中形态学的损伤。结论：丹参酮ⅡA对肠 IR 所致肺组织损伤有显著的剂量依赖性地保护作用,可能与其自由基清除作用、减轻中性粒细胞聚集及活化、抑制大量 NO释放有关。     

治疗癌痛从痰瘀虚论治浅析

目的:从痰、瘀、虚论治癌痛。方法:痰、瘀、虚即是癌痛形成的原因,又是癌痛的结果。结果:从痰、瘀、虚论治癌痛符合癌痛的病因病机。结论:祛痰活血,补虚泻实是治疗癌痛的主要方法。     

垂黄清脉冲剂干预治疗冠心病稳定型心绞痛热毒痰瘀证患者NF-κB信号通路的研究

目的 探讨垂黄清脉冲剂对冠心病稳定型心绞痛（热毒痰瘀证）患者NF-κB信号通路的调控。方法 本试验研究方法采用单盲、随机、对照法，选取就诊于宁夏医科大学总医院临床诊断为冠心病稳定型心绞痛（热毒痰瘀证）患者94例，将其随机分入对照组48例和试验组46例。两组均给予内科常规西药治疗，对照组加服银丹心脑通软胶囊，试验组加服垂黄清脉冲剂，疗程为3个月。于治疗前、后记录患者的基本情况，并采集血清样本，用酶联免疫吸附测定法（Elisa法）检测血清中TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1、ET-1、NO浓度水平进行对比，用SPSS22.0将数据进行统计学分析。结果 与治疗前比较，两组治疗后Gensini积分、血清中TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1、ET-1浓度水平均较治疗前显著降低，NO浓度水平显著升高（P < 0.05，P < 0.01），其中试验组显著程度尤为明显（P < 0.01）。与治疗后对照组比较，试验组Gensini积分、血清中TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1、ET-1浓度水平降低，NO浓度水平升高趋势更明显（P < 0.01）。结论 垂黄清脉冲剂治疗冠心病稳定型心绞痛（热毒痰瘀证）在改善患者症状；改善冠状动脉管腔狭窄程度；通过降低炎症标志物TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1的水平，减小ET-1/NO比值，从而对NF-κB信号通路进行部分调控，以有效抑制或截断血管内皮的炎症级联反应过程，清除炎症损伤因素以及保护血管内皮功能等方面均有显著优势，临床疗效确切值得肯定和推广应用。     

陆氏针灸“理气调经法”验案5则

<正>陆氏针灸疗法于2010年被纳入国家级非物质文化遗产名录,其作为"海派中医"的代表之一也同时被收录上海市非物质文化遗产名录。陆氏针灸在针刺、配穴、切诊方法以及经络理论等方面,均有不少独特的见解。陆氏针灸的创始人及核心人物为陆瘦燕先生。陆氏针灸的诸多理论形成,不仅凝聚了陆瘦燕先生毕生的心血,也同时囊括了包括朱汝功先生和很多受业弟子的努力挖掘和汇总、发展。谈月涓医师师从陆瘦燕先生,不仅将陆瘦燕先生