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21.
22.
Keiji Ogura Shigeki Fukuzawa Tomonori Habuchi Osamu Ogawa Osamu Yoshida 《International journal of urology》1997,4(6):561-565
Background :
In an attempt to determine the biological significance of nuclear morphometric findings, measurements of mean nuclear volume (MNV) and nuclear roundness factor (NRF) were compared to the immunoreactivityof p53 expression and proliferating cell nuclear antigen (PCNA) in human bladder cancer.
Methods :
MNV and NRF were measured using stereological methods. Expression of p53 and PCNA were determined by immunohistochemical staining. Specimens from 111 patients with previously untreated bladder cancer were analyzed.
Results :
The mean MNV was 235.8 ± 1 33.6 μm3 for the 81 patients with p53-labeling index (LI) less than 10% and 337.2 ± 141.0 μn3 for the 30 patients with p53 LI greater than 10% (P = 0.008). There was Resign if icant correlation between NRF and expression of p53. The mean MNV was 220.1 ± 1 20.5 μm3 for the 67 patients with PCNA LI less than 28% (the mean value of PCNA LI) and 328.9 ± 149.2 μm3 in 44 patients with PCNA LI greater than 28% (P= 0.0001). The mean NRF was 80.7 ± 4.2 for the 67 patients with PCNA LI less than 28%, and 82.3 ± 3.4 for the 44 patients with PCNA LI more than 28% (P= 0.04). Conclusion: Nuclear morphometric findings may reflect the proliferative potential of cancer eel Is of the bladder, as indicated by findings of immunostaining for p53 and PCNA. 相似文献
In an attempt to determine the biological significance of nuclear morphometric findings, measurements of mean nuclear volume (MNV) and nuclear roundness factor (NRF) were compared to the immunoreactivityof p53 expression and proliferating cell nuclear antigen (PCNA) in human bladder cancer.
Methods :
MNV and NRF were measured using stereological methods. Expression of p53 and PCNA were determined by immunohistochemical staining. Specimens from 111 patients with previously untreated bladder cancer were analyzed.
Results :
The mean MNV was 235.8 ± 1 33.6 μm
23.
How may clonality be assessed in human tumours? 总被引:1,自引:0,他引:1
24.
泪腺肿瘤中P53蛋白表达的意义 总被引:4,自引:0,他引:4
目的了解P53基因在泪腺肿瘤的表达及其临床病理学意义。方法应用免疫组织化学ABC法检测57例泪腺上皮性肿瘤患者和10例正常人泪腺组织中P53蛋白的表达情况,并与病理分级、复发相联系。结果10例正常人泪腺组织中P53表达全部为阴性;恶性肿瘤患者的表达率为48.6%,良性肿瘤中P53达率为18%,二者相比有显著性差异(P<0.025);复发患者阳性率为76.9%,明显高于未复发患为31.8%(P=0.015)。结论P53基因参与了泪腺肿瘤的发生发展并与肿瘤的分化和复发有关。 相似文献
25.
Chihaya Maesawa Gen Tamura Yasushi Suzuki Kaoru Ishida Kazuyoshi Saito Ryoichi Satodate 《Cancer science》1992,83(12):1253-1256
For the rapid and sensitive detection of p53 gene mutations in esophageal endoscopic biopsy specimens, we combined cell sorting with the polymerase chain reaction and single-strand conformation polymorphism (PCR-SSCP) analysis. Mutations in exons 5–8 of the p53 gene were investigated by FCR-SSCF analysis using 103 sorted nuclei obtained from each endoscopic biopsy specimen of 16 patients with esophageal cancer. DNAs extracted from their respective surgical specimens were investigated by a conventional method of PCR-SSCP analysis. Mutations in the biopsy specimens were detected in 6 of the 12 aneuploid tumors but in none of the 4 diploid tumors. After tumor cell enrichment by cell sorting, one mutation in exon 8 became apparent, which could not be detected from the surgical specimen by a conventional method of PCR-SSCP analysis. This method should improve the sensitivity of detecting p53 gene mutations, and provides additional information concerning the DNA ploidy pattern in the tumors. 相似文献
26.
30例新鲜胃癌标本,采用多聚酶链反应技术检测人类乳头瘤病毒16、18感染、并采用多聚酶链反应-单链构象多态性技术对p53基因突变进行检测。结果:HPV15阳性率为40.0%,未发现HPV18感染:P53基因突变率为50.0%,HPV16感染和P53基因突变同时存在者为16.7%。 相似文献
27.
VEGF和p53在胰腺癌中的表达及意义 总被引:1,自引:0,他引:1
目的 探讨胰腺癌组织中血管内皮生长因子 (VEGF)及p5 3蛋白的表达及其临床病理意义。方法 采用SP免疫组织化学方法 ,对 46例胰腺癌组织中VEGF及 p5 3蛋白的表达进行检测。结果 VEGF与 p5 3表达率分别为 65 .2 %和 5 8.7%。p5 3表达与VEGF表达呈明显正相关(P <0 .0 5 )。VEGF表达与胰腺癌淋巴结转移 (P <0 .0 5 )和远处转移 (P <0 .0 1)显著相关 ,而与肿瘤大小 ,病理学分级无关 ,p5 3表达与胰腺癌远处转移 (P <0 .0 5 )显著相关 ,而与肿瘤大小 ,病理学分级及淋巴结转移无关。结论 在胰腺癌中 ,VEGF表达与 p5 3蛋白的表达呈正相关 ,在胰腺癌的转移中起重要作用 相似文献
28.
目的 :研究启东区肝癌中 P53基因第七外显子转录水平的表达及其与患者临床病理特征间的关系。方法 :通过 RT- PCR确定 P53基因第七外显子在 47对肝癌标本中的表达 ,并分析其与肝癌患者临床病理特征间的关系。结果 :P53基因第七外显子在肝癌及其癌旁组织中均显示差异表达 ,其中 1 7例为上调表达 ,30例为下调表达 ,上下调表达之间差异显著 ;女性、年龄≥ 45岁、AFP( + )、HBs Ag( + )、癌栓 ( + )、肝硬化、病理 Edmondson's 级的病例中 P53基因第七外显子均呈显著下调表达 ;Edmondson's I级病例肝癌组织中 P53基因的 EI显著高于 Edmondson's 级病例。结论 :启东肝癌中 P53基因第七外显子转录水平上存在表达差异 ,且其异常表达与肝癌的临床病理特征有关 相似文献
29.
M. Eiro T. Katoh F. Ushikubi S. Narumiya T. Watanabe 《Clinical and experimental nephrology》2002,6(2):85-90
Background. Thromboxane (TX) A2 inhibition or prostaglardin (PGI2) infusion prevents ischemic acute renal failure (ARF) in animal models. However, the pathophysiological roles of the prostanoid
receptors in the development of ischemic ARF are not fully understood, partly because of the limited specificity of their
inhibitors or antagonists.
Methods. We investigated whether targeted disruption of the PGI2 receptor (IP) or TXA2 receptor (TP) genes conferred susceptibility to renal ischemic-reperfusion injury, using IP and TP knockout mice.
Results. Serum creatinine concentration in TP knockout mice was not significantly different from that in wild-type controls. There
were no significant histological differences between TP knockout and wild-type mice. Likewise, IP knockout mice showed no
significant differences from the wild-type controls in regard to creatinine concentration or histological damage.
Conclusions. Lack of TP or IP had no influence on postischemic ARF in mice, indicating that receptors for TXA2 or PGI2 may have minimal roles in the development of this mouse model of ischemic ARF.
Received: July 5, 2001 / Accepted: April 8, 2002 相似文献
30.
Martina Plísková Jan Vondrácek Borivoj Vojtesek Alois Kozubík Miroslav Machala 《Toxicological sciences》2005,83(2):246-256
Polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene (BaP), are carcinogens suggested to be involved in development of human cancer. Several recent studies have reported that PAHs can activate estrogen receptors (ER), either directly or indirectly by producing estrogenic metabolites. We hypothesized that the activation of ER by PAHs or their metabolites could induce cell proliferation in estrogen-sensitive cells. In the present study, we found that two PAHs, benz[a]anthracene (BaA) and BaP, can stimulate proliferation of human breast carcinoma MCF-7 cells at concentrations 100 nM and higher. This effect was ER-dependent, because it was blocked by the pure antiestrogen ICI 182,780. Although both PAHs partially inhibited S-phase entry and DNA synthesis induced by 17beta-estradiol, they stimulated S-phase entry when applied to MCF-7 cells synchronized by serum deprivation. This was in contrast with model antiestrogenic aryl hydrocarbon receptor ligand, 2,3,7,8-tetrachlorodibenzo-p-dioxin, which fully suppressed S-phase entry. BaP, which is a strong mutagen, was found to induce p53 tumor suppressor expression, a partial S-phase arrest and at higher concentrations also cell death. Pifithrin-alpha, a synthetic inhibitor of p53 activity, abolished both S-phase arrest and apoptosis induced by genotoxic PAHs, and it potentiated the proliferative effect of BaP. Thus, both genotoxic and nongenotoxic events seem to interact in the effects of BaP on cell proliferation. Taken together, our data indicate that both BaA and BaP can stimulate cell proliferation through activation of ER. The proliferative effects of these carcinogenic compounds might contribute to tumor promotion in estrogen-sensitive tissues. 相似文献