辛芷滴鼻剂质量标准研究

目的:建立辛芷滴鼻剂的质量控制标准.方法:采用高效液相色谱法对方中白芷所含欧前胡素进行含量测定,以十八烷基硅烷键合硅胶为填充剂,以甲醇-水(58:46)为流动相,检测波长300nm;采用薄层色谱法对方中所含白芷、辛夷进行定性鉴别.结果:欧前胡素进样量在0.116～2.320ug范围内,线性关系良好(r=0.9992),平均回收率100.6%.KSD为2.41%(n=6);薄层色谱显示方中白芷、辛夷专属性强,阴性无干扰.结论:该方法准确、可靠、重现性好,可作为辛芷滴鼻剂的质量控制标准.     

3种金属底板托槽喷砂处理前后粘结强度的比较及其意义

目的：探讨在人工唾液条件下临床正畸常用的3种金属底板托槽及脱落后经喷砂处理3种托槽的粘结强度,评价其粘结性能。方法：收集60颗因正畸需要拔除的上颌前磨牙,随机分为国产直丝弓新亚托槽（新亚）组、进口直丝弓MBT超薄托槽（MBT）组和日本直丝弓TOMY自锁托槽（TOMY）组,每组20颗。托槽脱落后将牙齿随机分为6组,分别粘结新的和脱落后经喷砂处理的3种托槽,每组10个。采用万能电子力学实验机测定每组的抗剪切强度,放大镜观察每组牙釉质表面粘结剂残留指数（ARI）,扫描电镜观察不同底板托槽及脱落后经喷砂处理后的托槽底板形貌。结果：在人工唾液条件下,TOMY组初次粘结新托槽和再次粘结新托槽的抗剪切强度高于新亚组和MBT组（P<0.05）,而新亚组的抗剪切强度与MBT组比较差异无统计学意义（P>0.05）;再次粘结脱落后经喷砂处理的3组托槽中,TOMY组抗剪切强度大于新亚组和MBT组（P<0.05）,而新亚组的抗剪切强度与MBT组比较差异无统计学意义（P>0.05）;TOMY组和MBT组脱落后经喷砂处理的托槽抗剪切强度较原托槽均增大（P<0.05）。各组的ARI比较差异无统计学意义（P>0.05）。扫描电镜观察,3种托槽底板均为纵横相间的网格,TOMY组更为致密,喷砂后砂粒嵌入网格中,TOMY组和MBT组砂粒更多嵌入倒凹处;再次喷砂后,砂粒嵌入范围增加,并且新亚组网底的网格结构出现破坏,其他2组未见明显异常。结论：3种底板托槽的粘结强度均能满足临床需要,其中TOMY组托槽的粘结强度优于其他2组。喷砂法处理脱落的TOMY和MBT托槽后可以提高托槽的粘结强度。     

TC—I型磁场扫描式理疗机对高血压病人血压的影响

目的：观察TC－I型磁场扫描式理疗机对高血压病人血压的影响。方法：用此机给病人每日磁疗1次,每次30分钟,14天为一个疗程,测量磁疗前后血压。结果：高血压病人经磁疗2次后血压明显下降,3次后达最低值,疗效持续至疗程终了。结论：此机对高血压病人有温和但明显的降压作用。     

木香CO2超临界萃取物中木香烃内酯、去氢木香内酯的鉴别与含量测定

用薄层色谱法（TLC）,以ψ（石油醚：乙酸乙酯：苯）＝70：5：15为展开剂,质量分数为5％香草醛硫酸为显色剂对木香超临界萃取物中的木香烃内酯与去氢木香内酯进行鉴别;并建立高效液相色谱法同时测定木香烃内酯与去氢木香内酯的含量法,色谱柱为Merck Lichrocart C18;流动相为ψ（甲醇：水）＝70：30;检测波长为225nm。测得木香烃内酯和去氢木香内酯的线性范围分别为0．4178－2．0888μg和0．3950－1．9752ug。回收率分别为100．3％和99．4％,方法简便,灵敏,快速。     

云芝糖肽的单糖组成分析

目的 对多孔菌科真菌云芝的活性成分云芝糖肽的单糖组成进行研究。方法 采用薄层色谱法和高效液相色谱－蒸发光用射检测器（HPLC－ELSD）联用技术。结果 云芝糖肽的单糖组成及摩尔比为：葡萄糖：D－甘露糖：半乳糖：木糖＝1：0.074:0.067:0.0178。结论 建立了云芝糖肽的单糖组成的检测方法,各单糖之间的比例与文献报道有所不同。     

氟化物转化细胞的超微结构观察

研究氟化物的致突变性和潜在致癌性,探讨其诱变机理。方法应用NaF诱导转化金黄地鼠胚胎细胞(SHE),以扫描和透射电镜观察其超微结构。结果该细胞群中少数细胞凋亡,大部分细胞则已逃脱凋亡转化成恶性细胞。结论氟化物对哺乳动物细胞可能具有诱变致癌性。     

微波提取法对槟榔中槟榔碱的提取率的影响

目的探讨提高槟榔中槟榔碱提取率的方法。方法用微波提取法提取槟榔碱,用薄层扫描仪检测以考察方法的可行性。结果槟榔碱的提取率明显提高。结论本方法具有快速、省时、节能等诸多优点,可适用于槟榔碱的工业化大生产。     

Scanning electron microscopic observation: three-dimensional architecture of the collagen in hepatic fibrosis rats

Background In the process of hepatic fibrosis, the accumulation of collagen fibers is strongly related to the hepatic function. The aim of this study was to investigate the three-dimensional architecture of the collagen network in the liver of rats with hepatic fibrosis. Methods Healthy adult male Wistar rats （n=32） were randomly divided into a control group （n=16） and a hepatic fibrosis group （n=16）. In the control group, the rats were treated with peanut oil while the rats in hepatic fibrosis group were treated for 10 weeks with 60% CCI4 diluted in peanut oil. The quantity of collagen fibers was detected by Western blotting; distribution of the collagen was detected by sirius red staining and polarized microscope; the three-dimensional architecture of collagen in the liver was observed under the scanning electron microscope after fixed tissues were treated with cell-maceration using NaOH. Statistical analysis was performed using the u test. Results The quantity of collagen fibers increased significantly in the hepatic fibrosis group. With the aggravation of hepatic fibrosis, collagen fibers gradually accumulated. They interlaced the reticulation compartment and formed a round or ellipse liver tissue conglomeration like a grape framework that was disparate and wrapped up the normal liver Iobule. The deposition of collagen fibers was obvious in adjacent hepatic parenchyma, especially around the portal tracts. Conclusion Our experiment showed the collagen proliferation and displays clearly the three-dimensional architecture of collagen fibers in rat liver with hepatic fibrosis by scanning electron microscope. It can provide a morphological foundation for the mechanisms of changed haemodynamics and portal hypertension in hepatic fibrosis.     

薄层扫描法测定玉台净注洗液中苦参碱的含量

目的:建立玉台净注洗液中苦参碱含量测定方法.方法:用薄层扫描法测定苦参碱的含量.结果:苦参碱在0.5～2.5 μg间线性关系良好,r=0.9996,平均回收率为98.3%.结论:所建方法简便、准确、重现性好,适用于该制剂的质量控制.     

前列舒乐泡腾片质量标准研究

目的:研究与修订前列舒乐泡腾片的质量标准。方法:化学鉴别法鉴别复方中药中的川牛膝和车前草;薄层色谱法鉴别中药复方中的蒲黄和黄芪。高效液相色谱法测定淫羊藿苷的含量,色谱柱为Polairs C18(150×4.6mm,5μm),流动相为乙腈-水(25∶75),检测波长270nm,流速1.0mL/min。结果:在薄层色谱中可鉴别出黄芪、蒲黄,无阴性干扰。淫羊藿苷在0.0493~0.4437μg范围内有良好的线性关系(r=0.9999),平均加样回收率(n=6)为99.33%(RSD=1.28%)。结论:该定性定量方法简便易行,重现性好,可作为前列舒乐泡腾片的质量标准基础。