Localized Sudeck's dystrophy and distal interphalangeal osteoarthritis of a finger: anatomicoradiologic study

After an injury to the right ring finger, a 44-year-old woman experienced pain in the distal interphalangeal joint. A subcutaneous mucoid cyst was noted near the joint. The x-ray films revealed no fracture but a narrowing of the joint space and a well-defined radiolucent area in the ulnar condyle of the middle phalanx. The biopsy specimen of this area excluded infection or tumor and revealed active osteogenic remodelling. These elements permitted a diagnosis of distal interphalangeal osteoarthritis that was associated with highly localized Sudeck's dystrophy (also called " parcellar algodystrophy"). This unusual case suggests the possibility that this dystrophy might be favored by an "osteoarthritic terrain" and might also play a role in the evolution of the osteoarthritis.     

Minimal interventions for weight control: a cost-effective alternative

Two studies were conducted to evaluate simpler, less intensive interventions for weight control which presumably would be more cost-effective and efficient than a "full-length" behavioral treatment program. In Study 1, participants in a minimal intervention (MI1) program who attended no regularly scheduled meetings and initially only received three simple verbal instructions about how to lose weight, lost an average of 11.1 lb. by 7-month follow-up. Subjects in three variations of a shortened, less intensive, 6-week behavioral weight loss program lost 7.8, 6.5 and 6.3 lb. but did not significantly differ from MI1 subjects in the amount of weight lost. In Study 2, MI2 subjects lost 5.5 lb. compared to subjects in two variations of a full-length program who lost 8.1 and 11.1 lb. by 6-month follow-up. Again, none of the groups significantly differed from each other in the amount lost. It was concluded that a minimal intervention program seems to produce weight loss and to be a cost-effective and efficient method for some subjects. The difference between the two minimal intervention programs may be related to the payment of a monetary deposit; a model for future research was presented to investigate simpler, less intensive interventions in combination with more complex ones in a "stepped-care" fashion.     

Gadd45β和Gadd45γ在亚砷酸钠所致的MIHA细胞周期改变中的作用

目的 探索Gadd45β和Gadd45γ在亚砷酸钠所致的MIHA细胞周期改变中的作用,为砷中毒的人群防治提供依据。方法 收集贵州省兴仁县雨樟镇交乐病区砷中毒人群以及格沙屯正常人群分别作为砷中毒组和对照组,实时荧光定量PCR检测各组人群Gadd45β和Gadd45γ基因mRNA表达水平。同时,分别以不同剂量亚砷酸钠、不同时间处理MIHA细胞,流式细胞术测定细胞周期改变情况,实时荧光定量PCR和免疫印迹法分别检测Gadd45β和Gadd45γ基因mRNA表达水平和蛋白表达情况。在上述实验基础上,针对Gadd45β和Gadd45γ基因分别设计的siRNA作用于染砷的MIHA细胞,反向验证Gadd45β和Gadd45γ基因在砷致细胞周期改变过程中的影响。统计学分析采用独立样本t检验比较两组间差异,采用单因素方差比较多组间差异。结果 人群实验研究发现,与对照组相比,砷中毒患者Gadd45β和Gadd45γ基因mRNA表达水平升高（t = 2.576,P = 0.011;t = 2.312,P = 0.022）;MIHA细胞中,随亚砷酸钠染毒剂量、染毒时间增加,细胞G2/M期比例明显升高（F剂量 = 340.136,P＜0.001;F时间 = 49.194,P＜0.001）;在相对较低亚砷酸钠浓度（低于20 μmol/L）、一定时间内（低于48 h）Gadd45β和Gadd45γ基因mRNA和蛋白表达水平随染砷剂量、染砷时间升高而升高,超过该浓度范围和作用时间后,出现表达下降的现象（Gadd45β:F剂量-蛋白 = 37.568,P＜0.001;F剂量- mRNA = 9.771,P＜0.001;F时间-蛋白 = 61.144,P＜0.001;F时间- mRNA = 46.366,P = 0.001;Gadd45γ:F剂量-蛋白 = 12.989,P = 0.001;F剂量- mRNA = 23.613,P＜0.001;F时间-蛋白 = 27.425,P＜0.001;F时间- mRNA = 37.969,P＜0.001）。转染siRNA分别下调Gadd45β和Gadd45γ的表达后,细胞周期都出现G2/M期比例下调（t = 3.053,P = 0.038;t = 14.47,P＜0.001）。结论 砷致Gadd45β和Gadd45γ基因表达水平升高在其诱导MIHA细胞出现G2/M期阻滞过程中发挥重要作用。     

急性白血病患者血清可溶性白细胞介素-2受体水平及临床意义

用双抗体夹心酶联免疫吸附试验检测 30例急性白血病患者血清可溶性白细胞介素 - 2受体 (SIL -2R)水平。结果　急性白血病均显著高于正常对照组 ,且和病情的严重程度密切相关 ,是判定预后的一个有用的指标。     

先天性心脏病儿童白细胞介素2活性及膜表面白细胞介素2受体的研究

目的 研究先天性心脏病（先心病）患儿的免疫功能状态。方法 采用间接免疫荧光检测膜表面白细胞介素2受体（Tac）、植物血凝素刺激后的膜表面白细胞介素2受体（Tac-a),MTT比色法检测白细胞介素2活性（IL－2）。结果 与健康儿童比较,先心病患儿IL－2活性和Tac-a都显著降低（P＜0．01）;与非紫绀型先心病患儿比较,紫绀型先心病患儿IL－2活性显著降低（P＜0．01）。结论 先心病患儿免疫功能缺陷可能是其易患感染性疾病的原因之一,增强免疫治疗对预防先心病患儿感染十分必要。     

大鼠心肌缺血再灌注时心肌细胞凋亡情况及缺血预处理对其影响

目的探讨缺血再灌注及缺血预处理对大鼠缺血再灌注心肌细胞凋亡的影响.方法制备大鼠缺血预处理(IP)、缺血再灌注损伤(I/R)模型,采用末端脱氧核苷酸转换酶介导的生物素平移缺口末端标记技术(TUNEL)检测心肌细胞凋亡情况;同时检测心肌梗死范围.结果I/R组细胞凋亡率(43.37±4.82%)高,IP组虽然也有一定的心肌细胞凋亡率(24.53±2.95%),但较I/R组明显降低(P＜0.001).IP组心肌梗死范围较I/R组明显减小.结论心肌缺血再灌注损伤可诱发或加重心肌细胞凋亡,IP能明显减少缺血再灌注诱导的心肌细胞凋亡的发生率,能明显减少心肌梗死范围,减轻缺血再灌注损伤;IP能减少心肌梗死范围、减轻缺血再灌注损伤的机理可能与其能明显减少心肌细胞凋亡有关.     

肺癌患者血清sIL-6R的测定

目的 :测定肺癌患者血清sIL 6R的水平。方法 :采取ELISA法 ,检测 40例各期肺癌患者及 2 0名健康成年人血清sIL 6R分泌水平。结果 :肺癌患者血清中sIL 6R水平高于正常人 (P <0 .0 5 ) ,化疗后患者血清中sIL 6R水平明显下降 (P <0 .0 1) ,并且也明显低于对照组 (P <0 .0 1)。结论 :肺癌患者血清sIL 6R水平随病情进展而升高 ,化疗后可下降。检测sIL 6R可为肺癌临床分期、疗效评价等提供参考     

Inhibition of Smad3 promotes the healing of rotator cuff injury in a rat model

To investigate the effect of inhibiting transforming growth factor‐β (TGF‐β1)/Smad2/3 signaling on rotator cuff (RC) healing. A bilateral supraspinatus tendon detachment‐repair model of Sprague‐Dawley (SD) rats was utilized. A total of 120 SD rats were randomly assigned to six groups and each group received the subacromial injection of normal saline, empty vectors, or lentiviral vectors containing small interfering RNA against TGF‐β1, Smad2, Smad3 at the bone‐tendon junction. Biomechanical and histological analyses were performed to evaluate bone‐tendon junction healing quality at 8 weeks after repair. Histologically, scar healing was found in all surgical groups. Animals with inhibited Smad3 exhibited better bone‐tendon junction structures with higher density, parallel orientation, and collagen fiber continuity than other surgical group animals. Immunohistochemistry revealed that the protein expression level of collagen I in animals with inhibited Smad3 was more prominent compared with all other surgical groups. Biomechanically, Animals with inhibited Smad3 showed better results in the maximum load at 4, 6, and 8 weeks after surgery compared with other surgical groups. Besides, C3H10T1/2 (Smad3?) cells increased TT‐D6 cell migration and tendon‐associated genes expression (scleraxis, tenascin C, collagen I) in coculture system. We conclude that inhibition of Smad3 promotes RC tendon healing in the rat supraspinatus model.     

Berberine plays a cardioprotective role by inhibiting macrophage Wnt5a/β-catenin pathway in the myocardium of mice after myocardial infarction

Myocardial infarction (MI) is one of the diseases with high fatality rate. Berberine (BBR) is a monomer compound with various biological functions. And some studies have confirmed that BBR plays an important role in alleviating cardiomyocyte injury after MI. However, the specific mechanism is unclear. In this study, we induced a model of MI by ligation of the left anterior descending coronary artery and we surprisingly found that BBR significantly improved ventricular remodeling, with a minor inflammatory and oxidative stress injury, and stronger angiogenesis. Moreover, BBR inhibited the secretion of Wnt5a/β-catenin pathway in macrophages after MI, thus promoting the differentiation of macrophages into M2 type. In summary, BBR effectively improved cardiac function of mice after MI, and the potential protective mechanism was associated with the regulation of inflammatory responses and the inhibition of macrophage Wnt5a/β-catenin pathway in the infarcted heart tissues. Importantly, these findings supported BBR as an effective cardioprotective drug after MI.