Culture and differentiation of chondrocytes entrapped in alginate gels

Summary We studied the response to culture conditions and the differentiative ability in suspension culture in alginate gels of resting chondrocytes from the preosseous cartilage of adult pig scapula. It was found that the maximum rate of chondrocyte duplication is reached at the fourth day in culture whereas the rate of proteoglycan synthesis and alkaline phosphatase expression do not gain a maximum value before the seventh day. During the culture time, the chondrocytes undergo differentiation as it is demonstrated by the alkaline phosphatase specific activity increase and by morphological criteria (hypertrophy, increase of the number of mitochondria per cell, increased endoplasmic reticulum, matrix vesicle production). The alginate gels can be easily dissolved to obtain cell populations in which the variation of cytosolic calcium concentration following a proliferative stimulus can be conveniently observed using the conventional procedure of Fura 2.     

大孔乙酸乙烯酯－异氰尿酸三烯丙酯共聚物珠体的合成与结构表征

以乙酸丁酯和２００＃汽油为混合致孔剂，采用悬浮聚合方法合成了大孔乙酸乙烯酯－异氰尿酸三烯丙酯共聚物珠体。对其孔结构、表面结构、溶胀性能和热稳定性进行了详细研究。     

钢丝悬吊综合矫治杯状耳畸形

目的　为先天性杯状耳行综合矫治 ,减少术后畸形复发提供一种新的可靠的手术方法。方法　在将杯状耳畸形软骨复位的同时 ,利用不锈钢丝将耳廓软骨上提悬吊于颅骨骨膜 ,共行手术 96例。结果　随访 5 2例 ,最长时间 3年余 ,证实效果满意可靠 ,达到了预期目的。结论　本术式是综合矫治轻中度杯状耳畸形的一个切实可行的方法     

Evaluation, using Salmonella typhimurium, of the mutagenicity of seven chemicals found in cosmetics

Six chemicals used as ingredients in cosmetics were evaluated for mutagenic activity in Salmonella typhimurium. Two of these ingredients, trans-4-phenyl-3-butene-2-one and 2,2′,4,4′-tetrahydroxybenzophenone, were mutagenic in the presence of rat liver S-9 towards strains TA100 and TA1537 respectively. An impurity found in some cosmetic products, N-nitrosodiethanolamine, was mutagenic to S. typhimurium stains TA1535 and TA100 in the presence of hamster-liver S-9 but not rat-liver S-9.     

Effects of sugar ester and hydroxypropyl methylcellulose on the physicochemical stability of amorphous cefditoren pivoxil in aqueous suspension

The improvement in physicochemical stability of amorphous cefditoren pivoxil (CDTR-PI) in aqueous suspensions by addition of sugar ester (SE) and hydroxypropyl methylcellulose (HPMC) was explained by prolonging the induction period prior to crystallization and the reduction in crystal peak intensity. Furthermore, the stabilizing effect of these additives in a multiple additive system was greater than in a single additive system. To determine the mechanism, by which these additives stabilized the amorphous CDTR-PI, we evaluated the surface states of CDTR-PI in suspension by measuring Raman spectra and zeta potential. The change in Raman spectra demonstrated that SE and HPMC interacted with CDTR-PI at the same interaction sites on CDTR-PI. The zeta potential reflected the adsorption phenomena of the additives and indicated that both SE and HPMC adsorbed onto particles of CDTR-PI with no apparent competitive interaction and the response was complementary. It was considered, based on this study, that HPMC and SE would stabilize amorphous CDTR-PI by different mechanisms; HPMC would mainly inhibit crystal growth by small amount of adsorption and SE would inhibit both crystal growth and nucleation by large amount of adsorption. This was considered to result in the hybrid effect in the multiple additive system.     

直接喉镜与电子喉镜下手术治疗会厌囊肿疗效比较

目的:探讨直接喉镜和电子喉镜下会厌囊肿摘除术的手术方法及各自的优缺点.方法:会厌囊肿患者72例,随机分为两组,治疗组38例行电子喉镜下手术,对照组34例行直接喉镜下手术.两组患者均在术前及术后1个月、5个月行电子喉镜检查,观察并比较两组术后疗效.结果:术后随访1～3 a,治疗组治愈34例、好转2例、未愈2例,治愈率89.4％;对照组治愈23例、好转6例,未愈6例,治愈率64.7％.两组患者治愈率比较,差异有统计学意义(P＜0.01).结论:电子喉镜下会厌囊肿摘除属微创手术,具有视野清晰、操作准确、创伤小、痛苦少、花费少等优点,值得临床推广.     

应用双线悬吊法行兔输精管吻合术的实验研究

目的 应用双线悬吊法行兔输精管吻合术,并探讨其安全性和有效性.方法 将30只雄性新西兰白兔随机分为3组,Ⅰ组在剪断输精管后即刻行双线悬吊法输精管吻合术,即以10 -0无创伤缝合线行输精管全层缝合两针,牵引缝线使两侧输精管断端无张力、无扭曲对合,牵引线缝出皮肤,打结固定于切口,10天后抽出牵引线.Ⅱ组和Ⅲ组分别在输精管...     

布地奈德混悬液雾化吸入配合猪肺磷脂防治新生儿呼吸窘迫综合征效果观察

目的探讨布地奈德混悬液雾化吸入配合猪肺磷脂（商品名：固尔苏）防治新生儿呼吸窘迫综合征（neonatal respiratory distress syndrome,NRDS）的效果。方法选择2009年9月至2010年9月我院新生儿病房收治的胎龄29～34周、生后2小时内入院的早产儿46例。按入院顺序单双号随机分成治疗组（A组）和对照组（B组）各23例,两组均给予综合治疗及猪肺磷脂120 mg气管内滴入,在此基础上,A组给予布地奈德混悬液0.5 mg雾化吸入,盐酸氨溴索针剂10 mg/kg静脉滴注,每8小时1次,连用5天;B组仅给予盐酸氨溴索针剂10 mg/kg静脉滴注,每8小时1次,连用5天。检测两组早产儿入院第1、2、3天动脉血氧分压（PaO2）、动脉血二氧化碳分压（PaCO2）水平及发生NRDS的例数。结果两组PaO2、PaCO2第1天差异无统计学意义,第2、3天差异有统计学意义（P〈0.05）;A组无NRDS发生,B组发生NRDS4例,发生率17%,两组比较差异有统计学意义（P〈0.05）。结论布地奈德混悬液雾化吸入配合猪肺磷脂能较快改善早产儿通气及换气功能,改善血气指标,提高机体供氧,有效预防NRDS的发生,降低早产儿的病死率。     

HPLC法测定雷奈酸锶干混悬剂的含量

目的建立雷奈酸锶干混悬剂含量的高效液相色谱测定方法 .方法采用Angilent Zorbax C18(4.6mm×250mm,5μm)色谱柱,流动相为0.2%(v/v)磷酸溶液-甲醇(85∶15)用三乙胺调节pH5.0,流速为1.0mL·min-1,检测波长234nm.结果在该色谱条件下,雷奈酸锶在4.96~248.00μg·mL-1浓度范围内线性关系良好,重复性试验RSD为0.71%,平均回收率为99.15%(RSD=0.57%).结论本法灵敏、准确、重复性好,可用于雷奈酸锶干混悬剂的质量控制.     

声带息肉的手术治疗

目的比较纤维喉镜法及支撑喉镜法治疗声带息肉的治疗效果,探讨不同手术方法的临床应用。方法回顾性分析我院2000年1月至2006年11月期间采用这两种手术方法治疗的声带息肉患者共300例,其中支撑喉镜150例,纤维喉镜150例,治疗后1个月复查电子喉镜,对比分析两组的疗效。结果支撑喉镜组有效率为98．67％,纤维喉镜组有效率为95．33％。经,检验,支撑喉镜组和纤维喉镜组相比无统计学意义,P〉0．05。结论两种手术方式治疗声带息肉的临床效果无明显差异,根据患者的具体情况和声带息肉特点选择相应的手术方法是保证治疗效果的重要步骤。