喘泰颗粒联合布地奈德治疗儿童哮喘急性发作的临床研究

目的观察喘泰颗粒联合布地奈德治疗儿童哮喘急性发作的临床效果。方法选择2016年10月-2019年10月在郑州大学第二附属医院治疗的哮喘急性发作患儿199例,随机分成对照组(100例)和治疗组(99例)。对照组雾化吸入吸入用布地奈德混悬液,1 mg/次,2次/d。治疗组在对照组治疗基础上口服喘泰颗粒,3 g/次,4次/d。两组均连续治疗7 d。观察两组患者临床疗效,同时比较治疗前后两组患儿用力肺活量(FVC)、最大呼气流量(PEF)、一秒用力呼气容积(FEV1)、免疫球蛋白G(IgG)、免疫球蛋白E(IgE)、免疫球蛋白M(IgM)、白介素-8(IL-8)和白介素-4(IL-4)水平,及临床症状改善时间。结果治疗后,对照组临床有效率为74.00%,显著低于治疗组的94.95%,两组比较差异具有统计学意义(P<0.05)。治疗后,两组患儿FVC、PEF和FEV1水平均明显升高(P<0.05),且治疗组患儿明显比对照组高(P<0.05)。治疗后,两组患儿IgG和IgM水平均明显升高(P<0.05),而IgE水平均明显降低(P<0.05),且治疗组患儿IgG、IgE和IgM水平明显比对照组好(P<0.05)。治疗后,两组患儿IL-8和IL-4水平均明显降低(P<0.05),且治疗组患儿明显低于对照组(P<0.05)。治疗后,治疗组患儿肺部哮鸣音、咳嗽、喘憋改善时间明显比对照组早(P<0.05)。结论喘泰颗粒联合布地奈德治疗小儿哮喘急性发作,可改善患儿临床症状,提高肺功能,改善机体免疫水平,临床疗效较好。     

胎肾细胞悬液对去卵巢骨质疏松大鼠骨生物力学性能的影响

目的探讨胎肾细胞悬液对去卵巢(OVX)骨质疏松大鼠股骨、腰椎骨生物力学性能的影响。方法取3月龄未交配健康SD雌性大鼠39只,随机分为4组,即A、B、C、D组。A组行假手术,B、C、D组行OVX手术。术后12周,A、B组给予生理盐水,C组给予尼尔雌醇,D组给予胎肾细胞悬液。药物治疗4周后,对左侧股骨、第2腰椎骨分别行三点弯曲力学试验、压缩力学试验,测量生物力学参数,检测血清雌二醇水平。结果与A组比较,B组大鼠股骨、腰椎骨的生物力学性能下降(P<0.05),血清雌二醇下降(P<0.01);与B组相比,C组、D组股骨、腰椎骨的生物力学性能明显改善,血清雌二醇的水平升高(P<0.05);C组与D组差异无显著性(P>0.05)。结论胎肾细胞悬液能够改善去卵巢大鼠的骨生物力学性能,提高血清雌二醇的水平,作用与尼尔雌醇相似。说明胎肾有防治去卵巢大鼠骨质疏松的作用。     

Systematic evaluation of suspension MDCK cells,adherent MDCK cells,and LLC-MK2 cells for preparing influenza vaccine seed virus

Suspension Madin–Darby canine kidney (MDCK) cells (MDCK-N), adherent MDCK cells (MDCK-C), and adherent rhesus monkey kidney LLC-MK2 cells (LLC-MK2D) were systematically evaluated for the preparation of influenza vaccine seed viruses for humans on the basis of primary virus isolation efficiency, growth ability, genetic stability of the hemagglutinin (HA) and neuraminidase (NA) genes, and antigenic properties in hemagglutination inhibition (HI) test of each virus isolate upon further passages. All the subtypes/lineages of influenza viruses (A(H1N1), A(H1N1)pdm09, A(H3N2), B-Victoria, and B-Yamagata) were successfully isolated from clinical specimens by using MDCK-N and MDCK-C, whereas LLC-MK2D did not support virus replication well. Serial passages of A(H1N1) viruses in MDCK-N and MDCK-C induced genetic mutations of HA that resulted in moderate antigenic changes in the HI test. All A(H1N1)pdm09 isolates from MDCK-C acquired amino acid substitutions at the site from K153 to N156 of the HA protein, which resulted in striking antigenic alteration. In contrast, only 30% of MDCK-N isolates showed amino acid changes at this site. The frequency of MDCK-N isolates with less than two-fold reduction in the HI titer was as high as 70%. A(H3N2) and B-Yamagata isolates showed high antigenic stability and no specific amino acid substitution during passages in MDCK-N and MDCK-C. B-Victoria isolates from MDCK-N and MDCK-C acquired genetic changes at HA glycosylation sites that greatly affected their antigenicity. When these cell isolates were applied to passages in hen eggs, A(H1N1), B-Victoria, and B-Yamagata viruses grew well in eggs, while none of the cell isolates of A(H3N2) viruses did. Thus, we demonstrate that MDCK-N might be useful for the preparation of influenza vaccine seed viruses.     

Approche exploratoire du discours autour de la suspension dans le cadre du body art

This semantic analysis led by a discourse analysis software (Tropes) proposes a reflection concerning a speech about body art's practice: suspension. It is an exploratory research presenting an analysis of a text written by a young man practicing the suspension. The purpose being to focus on the speech concerned the act of suspension.     

Influence of Benzyl Benzoate as Oil Core on the Physicochemical Properties of Spray-Dried Powders from Polymeric Nanocapsules Containing Indomethacin

Estradiol transdermal application still represents a relevant topic due to the recent controversies about hormon replacement therapy, its increasing potential in the treatment of skin aging and wound healing, and the new use of its derivatives in the transdermal contraceptive treatment. The aim of our work was to verify if fresh or frozen rabbit ear skin can be a suitable skin model to study in vitro estradiol transdermal absorption. Fresh rabbit ear skin demonstrated a reasonable model of human epidermis in the investigation of estradiol permeation starting from both solutions and commercial patches.     

几种含氯消毒剂对金黄色葡萄球菌杀菌效果的研究

目的：通过实验室试验,探讨不同有机干扰物条件下几种含氯消毒剂杀灭金黄色葡萄球菌的最低有效剂量。方法：依据《消毒技术规范》（2002年版）,应用悬液定量杀菌试验。结果：在有效氯含量为250 mg/L、3%牛血清白蛋白条件下,三氯异氰尿酸和优氯净分别作用7.5 min和15 min可杀灭金黄色葡萄球菌,平均杀灭对数值均〉5.00;某消毒泡腾片和某84消毒液分别作用22.5 min和45 m in均不能杀灭金黄色葡萄球菌,杀灭对数值分别为4.57和3.13。在有效氯含量为50 mg/L、0.3%牛血清白蛋白条件下,三氯异氰尿酸、优氯净、某消毒泡腾片和某84消毒液分别作用7.5、15、22.5和45 min可杀灭金黄色葡萄球菌,平均杀灭对数值均〉5.00。结论：单一成份含氯消毒剂在3%和0.3%牛血清白蛋白条件下,最低有效浓度分别为250 mg/L和50 mg/L,而加入了稳定剂或泡腾剂的效果较差。     

硝普钠对保存期间悬浮红细胞变形性的作用研究

目的 探讨硝普钠对保存期间悬浮红细胞变形性的影响.方法 来源于血站的50袋悬浮红细胞,200mL/袋,在保存期的第1、3、5、7、14、21、28、35天(D1、D3、D5、D7、D14、D21、D28、D35)共8个时间点,各取20 mL,分成4管(5 mL/管),其中,1管不做处理为对照组(A组),另外3管分别添加硝普钠,配置成终浓度为B组1 μmol/L、C组5μmol/L和D组10 μmol/L.检测4组的红细胞变形指数、聚集指数和刚性指数.结果 与A组红细胞变形指数比较,B组和D组差异无统计学意义(P＞0.05),C组明显降低(P＜0.05);与A组聚集指数和刚性指数比较,B、C、D3组之间差异无统计学意义(P＞0.05).结论 浓度为5μmol/L的硝普钠能够改善悬浮红细胞的变形指数,但对聚集指数和刚性指数无明显影响.     

Polymer incorporation method affects the physical stability of amorphous indomethacin in aqueous suspension

This study reports the potential of different polymers and polymer incorporation methods to inhibit crystallisation and maintain supersaturation of amorphous indomethacin (IND) in aqueous suspensions during storage. Three different polymers (poly(vinyl pyrrolidone) (PVP), hydroxypropyl methylcellulose (HPMC) and Soluplus® (SP)) were used and included in the suspensions either as a solid dispersion (SD) with IND or dissolved in the suspension medium prior to the addition of amorphous IND. The total concentrations of both IND and the polymer in the suspensions were kept the same for both methods of polymer incorporation. All the polymers (with both incorporation methods) inhibited crystallisation of the amorphous IND. The SDs were better than the predissolved polymer solutions at inhibiting crystallisation. The SDs were also better at maintaining drug supersaturation. SP showed a higher IND crystallisation inhibition and supersaturation potential than the other polymers. However, this depended on the method of addition. IND in SD with SP did not crystallise, nor did the SD generate any drug supersaturation, whereas IND in the corresponding predissolved SP solution crystallised (into the recently characterised η polymorphic form of the drug) but also led to a more than 20-fold higher IND solution concentration than that observed for crystalline IND. The ranking of the polymers with respect to crystallisation inhibition potential in SDs was SP ≫ PVP > HPMC. Overall, this study showed that both polymer type and polymer incorporation method strongly impact amorphous form stability and drug supersaturation in aqueous suspensions.     

CO2激光喉显微手术与电子喉镜手术治疗声带息肉效果比较

目的 比较支撑喉镜下CO₂激光显微手术与电子喉镜下手术治疗声带息肉的临床疗效。方法 选取2014年1月至2015年1月芜湖市第二人民医院收治的144例声带息肉行支撑喉镜下CO₂激光显微手术患者(激光组)及同期门诊75例电子喉镜下手术的声带息肉患者(喉镜组),比较分析两组患者的临床治疗效果。结果 激光组显效120例,有效19例,无效5例,总有效率为96.52%;喉镜组治愈60例,有效12例,无效3例,总有效率为96.00%。两组总有效率比较,差异无统计学意义(P>0.05)。结论 声带息肉的手术方式应根据患者的自愿、声带息肉的基底部以及声门暴露情况选择不同的手术。