记号笔代替无菌物品缸标签的方法

无菌物品缸在临床外科处置室广泛使用,包括无菌纱布缸,棉球缸、呋喃西林纱布缸、碘伏棉球缸等,各种无菌物品缸每日刷洗后需要高压蒸汽灭菌使用,并且做好标记。传统方法为打印相关物品名称后利用透明胶带粘贴在缸子盖上,经反复高压后字迹变淡、变黄,残留胶布印不易清洁,刷洗时常留有划痕。     

Detection of hyphal fusion in filamentous fungi using differently fluorescence-labeled histones

Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted merged fluorescence (HAMF) assay for the investigation of hyphal fusion between vegetative mycelia. For this purpose, two reporter vectors were constructed encoding the histone proteins HH2B or HH2A fused at their C terminus either with the cyan or yellow fluorescent protein, respectively. The chimeric proteins generate fluorescently labeled nuclei and thus enable the distinction between different strains in a mycelial mixture. For example, hyphae with nuclei that show both cyan as well as yellow fluorescence indicate the formation of a heterokaryon as a result of hyphal fusion. To test the applicability of our HAMF assay, we used two S. macrospora developmental mutants that are supposed to have reduced hyphal fusion rates. The simple and efficient HAMF assay described here could detect even rare fusion events and should be applicable to a broad range of diverse fungal species including those lacking male or female reproductive structures or asexual spores. Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. Christine Rech and Ines Engh contributed equally to this work.     

Use of controlled fascial tension and an adhesion preventing barrier to achieve delayed primary fascial closure in patients managed with an open abdomen

Open management of the abdomen has become an accepted technique for both the treatment and the prevention of abdominal compartment syndrome. It has also gained popularity as a treatment option in situations requiring multiple laparotomies such as uncontrolled intra-abdominal infections and severe abdominal injury necessitating damage control surgery. A significant number of patients managed with the open abdomen technique are unable to undergo complete abdominal wall closure and consequently develop large, complex anterior abdominal wall hernias. We report the use of a controlled fascial tensioning device, the Wittmann Patch (Starsurgical, Inc, Burlington, WI), in combination with an adhesion preventing barrier to allow for unhindered sequential medial advancement of the fascia toward the midline. The use of these 2 devices together may lead to a higher incidence of fascia-to-fascia abdominal wall closure than the use of fascial tension alone.     

实施零库存理念管理模式,优化医院药库工作流程

目的:为医院药库实施零库存理念管理提供参考。方法:以我院药库管理经验为蓝本,探讨实施零库存理念管理的模式。结果与结论:我院药库实施零库存理念管理模式后,药品周转次数达每季度8~9次,大大降低了药品的储存成本,极大地盘活了流动资金,使我院药品资金降至仅占医院流动资金的15%左右。因此,建立适合院情的库存管理模式,应用零库存理念管理医院药库,对降低医院运营成本、提高工作效率具有重大意义。     

无菌接合机在白细胞过滤中对血液质量的影响

目的探讨无菌接合机(SCD)在白细胞过滤中对血液成分质量的影响。方法收集正常献血者标本20份,保存1、10、20和30d。采用SCD法和传统针穿刺法对标本进行处理,测定SCD处理组、针穿刺组和无处理对照组的游离血红蛋白含量,计算溶血率,采用统计学方法比较差异和两种方法的相关性。结果3组血液标本不同保存时间的游离血红蛋白和溶血率的差异无统计学意义(P>0.05),SCD法和针穿刺方法两组溶血率的相关系数>0.9。结论SCD法和传统的针穿刺法在接合白细胞过滤器的应用上对血液成分质量的影响无差异。     

红外线照射预防外周中心静脉置管术后无菌性静脉炎的研究

目的探讨红外线照射对外周中心静脉置管术(PICC)后无菌性静脉炎的预防与控制效果。方法对122例行PICC术的住院患者,随机分为两组,对照组PICC术后患者给予常规护理,试验组PICC术后患者在常规护理的基础上行早期红外线照射,观察两组行PICC术后无菌性静脉炎的发生率和相关因素以及置管后的成功率。结果试验组置管后的成功率明显高于对照组,无菌性静脉炎发生率为0,对照组无菌性静脉炎发生率为21.9%;而无菌性静脉炎的发生与插管不畅、反复送管的关系密切。结论早期行红外线照射可以预防无菌性静脉炎的发生,提高置管后的成功率,保证治疗有效进行,减轻患者痛苦和降低患者的医疗费用。     

Sterile production and mechanical reliability of autoclaves

The failure rate of a steam autoclave used in a hospital sterile-fluid production unit has been analysed by fitting observed breakdowns to the Weibull distribution. The results indicate a rapid initial breakdown rate which clearly represents an unsatisfactory situation for the pharmacy department trying to provide a consistent, reliable service. It is concluded that greater attention should be paid to auctoclave reliability if cost-effective sterile-fluid production is to be achieved     

沟通在无菌物品全程优质供应服务中的应用

目的 以沟通的方式发挥消毒供应科与各临床科室的协同作用,达到无菌物品管理工作全程质量监控良好运行.方法 2004年1月-2006年9月将灭菌专业知识编排、打印成彩页逐期下发科室,将无菌物品全程质量监控、管理工作、安全使用注意事项等纳入日常沟通工作中.结果 经过沟通起到了与科室间无菌物品供应及需求的和谐,畅通了无菌物品的下送全程优质保供服务管理工作.结论 消毒供应科与各临床科室在无菌物品全程质量管理中的沟通十分重要.     

针尖穿刺力实验室间比对验证

本文主要通过组织8个实验室进行一次性使用无菌注射针“针尖刺穿力”的能力比对验证实验,验证该项目检验方法的有效性和可比性,以及各检验实验室检验该项目的差异。