复方螺旋藻片对高血脂症小鼠血脂的影响

目的：观察复方螺旋藻片（FST）对高脂血症小鼠血脂的影响。方法：用FST1.5mg/kg,3g/kg分别灌胃给予高脂血症小鼠,连续14d后,眼眶取血测血脂。结果：与高脂血症模型对照组相比,FST高、低剂量能显著降低血清TC、TG和LDL－C水平,明显升高血清HDL－C水平与HDL－C／TC比值。同时FST还能升高血清apoAI并降低apoB(但FST 1.5g/kg对apoB无明显影响）。结论：FST能明显降低高脂血症小鼠血脂水平,并对载脂蛋白有一定的调节作用。     

螺旋藻对实验性矽肺大鼠体内抗氧化水平的作用

为研究螺旋藻对实验性矽肺大鼠体内抗氧化水平的作用,实验设空白对照组、染尘对照组和染尘螺旋藻实验组,用非暴露式气管注入法复制大鼠矽肺模型,饲养１２周。结果表明：螺旋藻能显著提高染尘大鼠超氧化物歧化酶和谷胱甘肽过氧化物酶抗氧化酶的活性,降低肺组织中丙二醛的含量和支气管肺泡灌洗液中乳酸脱氢酶、酸性磷酸酶的含量。结果提示：（１）螺旋藻具有提高实验性矽肺大鼠体内抗氧化水平的作用;（２）螺旋藻在一定程度上能延缓或抑制矽肺病变的发展;（３）螺旋藻提高机体抗氧化水平的作用机理可能与其含有的微量元素Ｓｅ和β胡萝卜素等营养成分有关。     

螺旋藻对DMBA-TPA诱发地鼠口腔癌血管生成的研究

目的:通过研究螺旋藻对金黄地鼠口腔癌血管生成的影响,探讨螺旋藻对口腔癌的阻断作用机理,为临床应用提供理论依据.方法:91只金黄地鼠随机分为5组,阳性对照组及螺旋藻3个不同剂量的用药组,于地鼠左侧颊囊涂二甲基苯并蒽和12-O-四葵酰基-佛波-13-乙酸酯溶液,螺旋藻用药组在涂12-O-四葵酰基-佛波-13-乙酸酯的同时给予不同剂量(200mg/kg;400 mg/kg;800 mg/kg)的螺旋藻混悬液灌胃治疗,同时设阴性对照组.实验结束处死动物进行肿瘤血管生成相关指标的检测.结果:螺旋藻可显著降低地鼠口腔癌的血管密度,表明螺旋藻对血管生成有一定的抑制作用;但是对血管内皮生长因子没有影响.结论:通过抑制新生血管生成,螺旋藻可以阻断口腔癌前病变的进一步发展,但其抑制新生血管的机制需进一步探讨.     

Effects of Spirulina supplementation on obesity: A systematic review and meta-analysis of randomized clinical trials

ObjectiveEvidence has suggested that Spirulina supplementation may affect anthropometric indices. Therefore, a systematic review and meta-analysis was performed to summarize published randomized clinical trials which assess the effect of Spirulina supplementation on obesity.SettingPertinent studies were identified using Embase, Scopus, ISI Web of Science, PubMed and Cochrane library databases up to May 2019. Mean Differences (MD) were pooled using a random-effects model. Heterogeneity, sensitivity analysis and publication bias were reported using standard methods.ResultsResults of 5 studies (7 treatment arms) showed a significant reduction in weight (MD: -1.56 Kg, 95% CI: -1.98 to -1.14) after Spirulina supplementation. Subgroup analysis based on health status revealed that weight change in obese subjects (MD: -2.06 Kg, 95% CI: -2.45 to -1.68) was greater than overweight participants (MD: -1.28 Kg, 95% CI: -1.62 to -0.93) following Spirulina supplementation. Also, pooled analysis showed that Spirulina supplementation led to a significant reduction in body fat percent (MD: -1.02, 95% CI: -1.49 to -0.54) and waist circumference (MD: -1.40, 95% CI: -1.40 to -1.39), but not in body mass index and waist to hip ratio.ConclusionSpirulina supplementation significantly reduces body weight, especially in obese individuals.     

藻参胶囊的辐射防护作用

统具有明显的保护作用.     

谷氨酸脱氢酶法测定螺旋藻蛋白质

建立一种用谷氨酸脱氢酶测定螺旋藻蛋白质的方法,将螺旋藻蛋白质消化成（ＮＨ４）２ＳＯ４,再用谷氨酸脱氢酶动力法在自动生分析仪上测定（ＮＨ４）２ＳＯ４浓度,计算螺旋藻蛋白质含量。     

螺旋藻多糖对糖尿病小鼠抗氧化能力的影响

腹腔注射四氧嘧啶（ＡＬＸ）２００mg/kg建立糖尿病模型小鼠,并随机分为４组,分别用螺旋藻多糖（ＰＳＰ）１００mg/kg、２００mg/kg及阳性对照药优降糖２mg/kg灌胃给药,糖尿病模型对照组则给等容积生理盐水,连续１４d后,分别测定各组小鼠血糖、血清ＳＯＤ活性、ＭＤＡ含量及全血ＧＳＨ－Ｐx活性与ＧＳＨ含量。结果与糖尿病模型对照组相比,ＰＳＰ能使ＡＬＸ所致糖尿病小鼠血糖降低（Ｐ＜０.０１）;血清ＳＯＤ活性、全血ＧＳＨ－Ｐx活性及ＧＳＨ一显著回升（Ｐ＜０.０１）;血清ＭＤＡ含量明显下降（Ｐ＜０.０１）。结果表明ＰＳＰ能降低ＡＬＸ性糖尿病小鼠高血糖并显著增强其抗氧化能力。提示ＰＳＰ降血糖作用的机制可能与其增强糖尿病鼠抗氧化能力有关。     

螺旋藻硒多糖对小鼠免疫功能的影响

本文研究了螺旋藻中的硒多糖 (selenium polysaccharide,Se PS)抑制肿瘤细胞和对小鼠免疫功能的影响。发现硒多糖对肿瘤细胞的抑制率均大于相同剂量的多糖 (PS)。高浓度的 Se PS和 PS(10 0 mg· kg-1)处理能显著提高小鼠的脾指数 ;PS和 Se PS都能显著提高 CY处理后的胸腺指数和脾指数 ;剂量 5 0 mg· kg-1以上的 Se PS或 PS与 Con A协同作用能显著刺激小鼠淋巴细胞增殖 ;Se PS两个剂量 (5 0 mg· kg-1和 10 0mg· kg-1) NK细胞对靶细胞的杀伤率分别达到 33.2 %和 4 0 .8% ,与对照组 (19.0 % )相比 ,达到极显著差异 ,PS(5 0 mg· kg-1)达到显著差异 (2 9.9% ) ,PS(10 0 mg· kg-1)达到极显著差异 (38.0 % )     

Antineoplastic activity of γ-linolenic acid extract from Spirulina platensis on HepG2 cells and its inhibition effect on platelet aggregation

Spirulina platensis (SP) is well recognised for its abundant unsaturated fatty acids, especially γ-linolenic acid (GLA). In this study, GLA was extracted from SP. Antineoplastic and antithrombotic activities of GLA were evaluated by measuring the concentrations of reactive oxygen species (ROS) and malondialdehyde, as well as inducing apoptosis in HepG2 human hepatoma cells and diphosphatase-induced platelet aggregation inhibition assay, respectively. The study suggests that the GLA extraction from SP using ethanol achieved a higher extraction yield of 8.3 g kg^?1 ± 0.17 g kg^?1 (GLA/dry biomass) and inhibited cell proliferation in a dose- and time-dependent manner. GLA extract (250 µM) strongly induced ROS generation and lipid peroxidation as well as apoptosis. The extract also caused a concentration-dependent antithrombotic effect on platelet aggregation in vitro. Thus, the GLA extract from SP can be applied in health care and functional foods.     

Effects of different probiotic combinations on the components and bioactivity of Spirulina

Spirulina acts as a good dietary nutritional supplement. However, few research studies have been conducted on its fermentation. Three groups of probiotic combinations, lactic acid bacteria, Bacillus strains, and their mixture, were used to investigate Spirulina fermentation. The results showed that lactic acid bacteria significantly increased the content of amino acids and the ratio of essential amino acids to total amino acids in the fermented Spirulina, compared with the unfermented Spirulina, and this trend was enhanced by the strains' mixture. However, compared to unfermented Spirulina, the amino acid levels were significantly decreased after fermentation with Bacillus strains and so was the total free amino acid and essential amino acid content. Fermentation significantly reduced the contents of the offensive components of Spirulina, with significant differences among the three mixed bacterial treatments. Moreover, Bacillus strain fermentation increased the contents of flavonoids and polyphenols compared to the unfermented Spirulina, and significantly enhanced 1,1-diphenyl-2-trinitrophenylhydrazine free-radical scavenging ability and total antioxidant ability. On the contrary, treatments with lactic acid bacteria and the mixture of lactic acid bacteria and Bacillus strains endowed the fermented supernatants with good antibacterial ability. The results showed that probiotic fermentation has a good effect on Spirulina and can serve as a new procedure for developing new Spirulina-containing food items.