分子排阻色谱法测定螺旋藻多糖纯度和分子质量

使用分子排阻色谱法，用已知平均分子质量的硫酸化葡聚糖作为标准，分别用5种不同的流动相，在Shodex Sugar KS柱上测定螺旋藻多糖的纯度以及分子质量，使用线性回归得出校正曲线。并且通过对结果进行多种方法学的考查，选取最佳的分析条件。     

Development of coaxial alginate-PCL nanofibrous dressing for controlled release of Spirulina extract

Spirulina has widely been highlighted as a source of bioactive material that can be impregnated into dressing materials. The aim of this study was to widen the application fields of Spirulina extract-containing nanofiber, which has been suggested as an attractive dressing material in several previous studies. The bioactivity release pattern, water absorbance, and mechanical strength must be controllable. Spirulina extract was physically impregnated inside a nanofiber without significant chemical bonding to polycaprolactone or alginate polymers. This led to an initial burst and continual release of bioactive molecules from the nanofiber. By altering the concentration of Spirulina extract, mechanical strength and water absorbance were controllable. In addition, the dressing patch showed no cytotoxicity towards human epithelial cells, not causing skin-irritation. This indicates that the coaxially fabricated patch is a controllable dressing material that can be customized to have a specific mechanical strength, water absorbance, and bioactive release pattern, making it suitable for wide applications.     

复合螺旋藻多糖对人乳腺癌MB-231细胞株体外抗肿瘤作用研究

目的:研究复合螺旋藻多糖对体外培养的人乳腺癌MB-231肿瘤细胞的抑制作用及可能的作用机制。方法:以人乳腺癌MB-231细胞株为研究对象,将螺旋藻多糖(PSP)与银杏叶提取物(GBE)按不同比例复合,并用不同浓度的复合制剂分别对细胞作用后,分别在48h、72h后,显微镜下观察细胞形态学变化,MTT法测其生长抑制率。48h后,PI及AnnexinV-FITC/PI染色,用流式细胞仪测其细胞周期及凋亡率。结果:复合螺旋藻多糖对人乳腺癌MB-231细胞均有不同程度的抑制作用,复合组优于单一成分组,抑制生长效果与时间和剂量呈依赖关系;将人乳腺癌MB-231细胞阻滞于G0/G1期,抑制肿瘤细胞的有丝分裂;促进肿瘤细胞凋亡,与空白组相比差异显著。结论:复合螺旋藻多糖通过阻断细胞周期达到抑制体外培养的人乳腺癌MB-231细胞的生长的目的,具有较好的抗肿瘤活性。     

In vitro and in vivo safety assessment of edible blue-green algae, Nostoc commune var. sphaeroides Kützing and Spirulina plantensis

Blue-green algae (BGA) have been consumed as food and herbal medicine for centuries. However, safety for their consumption has not been well investigated. This study was undertaken to evaluate in vitro and in vivo toxicity of cultivated Nostoc commune var. sphaeroides Kützing (NO) and Spirulina platensis (SP). Neither NO nor SP contained detectable levels of microcystin (MC)-LA, MC-RR, MC-LW and MC-LR by LC/MS/MS. Cell viability remained ∼70-80% when HepG2 cells were incubated with 0-500 μg/ml of hexane, chloroform, methanol and water-extractable fractions of NO and SP. Four-week-old male and female C57BL/6J mice were fed an AIN-93G/M diet supplemented with 0%, 2.5% or 5% of NO and SP (wt/wt) for 6 months. For both genders, BGA-rich diets did not induce noticeable abnormality in weight gain and plasma alanine aminotransferase (ALT) and aspartate aminotransferase concentrations except a significant increase in plasma ALT levels by 2.5% NO supplementation in male mice at 6 month. Histopathological analysis of livers, however, indicated that BGA did not cause significant liver damage compared with controls. In conclusion, our results suggest that NO and SP are free of MC and the long-term dietary supplementation of up to 5% of the BGA may be consumed without evident toxic side-effects.     

铅的神经毒性及螺旋藻的拮抗效应

目的探讨螺旋藻对铅致神经毒性的拮抗作用。方法采用口服醋酸铅(PbAC2)溶液方法建立小鼠铅中毒模型,用螺旋藻灌胃进行实验性治疗。持续6周后,实验检测小鼠学习、记忆成绩和抗疲劳能力,并测定脑组织和血浆中丙二醛(MDA)含量。结果与对照组相比,模型组的学习、记忆能力下降,抗疲劳能力降低;MDA含量增高,差异均有统计学意义(P0.05或P0.01)。与模型组相比,治疗组的学习、记忆能力和抗疲劳能力又得到改善,MDA含量也降低,差异均有统计学意义(P0.05或P0.01)。结论铅可降低小鼠的学习、记忆能力和抗疲劳能力,而螺旋藻对铅导致的神经毒性有一定的拮抗作用。     

螺旋藻胶囊的药理与毒理研究

螺旋藻胶囊由螺旋藻（ｓｐｉｌｕｉｎａｐｌａｌｅｎｓｉｓ）的干粉组成。实验结果表明，螺旋藻能明显延长小鼠负重游泳时间，降低小鼠血清总胆固醇，对Ｃｏ（６０）所致小鼠白细胞减少有明显升高作用，有提高小鼠失血后的白细胞及小鼠碳廓清速率。长期毒性实验表明，螺旋藻粉无明显毒副作用，急性毒性实验未测出小鼠致死剂量。     

Protective effect of Spirulina on lead induced deleterious changes in the lipid peroxidation and endogenous antioxidants in rats

The present study aims to investigate the protective effect of Spirulina on lead-induced changes in the levels of lipid peroxidation and endogenous antioxidants in liver, lung, heart, kidney and brain of rats. Levels of elemental lead were also measured in the organs of rats in all experimental groups. In the liver, lung, heart and kidney of lead-exposed animals, there was a significant (p < 0.001) increase in the lipid peroxidation and a decrease in the levels of endogenous antioxidants. Although, Spirulina did not affect the deposition of lead in organs apart from the brain, simultaneous administration of Spirulina to lead exposed animals significantly (p < 0.001) inhibited lipid peroxidation and restored the levels of endogenous antioxidants to normal. To conclude, Spirulina had a significant effect on scavenging free radicals, thereby protecting the organs from damage caused by the exposure to lead. Further more, Spirulina showed a significant (p < 0.05) decrease in the deposition of lead in the brain.     

螺旋藻藻蓝蛋白的研究进展

本文综述了近年来螺旋藻藻蓝蛋白的提取、纯化、理化特性、生理活性及产品开发等方面的研究进展情况，并提出了未来几年研究的主要方向在于提取工艺、药品开发和质控方法。     

盐泽螺旋藻多糖SP抑制肿瘤细胞的研究

热水抽提法提取盐泽螺旋藻多糖,DE-52柱层析纯化,Sephadex G-100柱层析鉴定其纯度,获得纯化组分SP。通过体外细胞实验发现纯化的多糖可以选择性的抑制肿瘤细胞的生长。通过对肿瘤细胞形态的观察,发现在SP作用下,细胞出现凋亡小体;结合Hochest-33342染色,初步认为多糖是通过促进肿瘤细胞的凋亡来抑制肿瘤细胞的生长。     

钝顶螺旋藻糖缀合物SPPA-1的理化性质及活性的研究

目的　研究螺旋藻多糖中的均一组分SPPA 1的分离方法、糖组成、分子量及生物活性。方法　采用丙酮分部沉淀 ,三氯乙酸去蛋白 ,再经SephadexG 75和CM SephadexC 5 0柱色谱得到SPPA 1,用气相色谱法测定单糖组成 ,GPC法测定分子量 ,IR和NMR法确定糖苷键类型。结果　经高压液相色谱和毛细管电泳鉴定为均一性组分。其总糖含量为 91 70 % ,N含量为 0 96 % ,分子量为 6 9 0 0× 10 4 Da。糖组成分析表明其由单一的葡萄糖组成 ,红外光谱、核磁共振1HNMR谱分析表明 ,其单糖类型为α 吡喃型。ESR研究表明SPPA 1具有清除O·2 自由基活性。结论　SPPA 1为一具抗氧化活性的葡聚糖