广西槐种质资源调查

目的:开展广西槐种质资源调查,为广西槐种质资源的综合评价及筛选优良种质奠定基础。方法:采用实地走访、文献资料查阅相结合的方法对广西槐树种质资源进行系统的调查研究。结果:(1)广西槐树主要分布在全州、兴安、阳朔、富川、恭城等县(市),位于北纬N24°28'23″~N26°22'15″,东经E109°22'06″~E111°16'21″的区域内,以海拔为104~502 m最为常见。(2)不同分布区中不同类型槐树的叶片性状指标,植株大小、槐米质量方面差异较大,其中全州七一栽培的双季金槐在形态大小、叶片性状、槐米产量等方面明显优于其他分布区。(3)广西槐树适应性强,对温度、土壤及降水量的适应范围较宽。(4)广西槐树伴生植物的种类较少,但优势科的组成较为突出,含种数最多的是菊科其次是禾本科、车前科、唇形科、大戟科等优势科较为突出。结论:基本清楚了广西槐树的地理分布特点、生长特性及栽培槐树的类型,为广西槐树良种选育提供科学依据。     

桃金娘叶的化学成分研究

目的研究桃金娘Rhodomyrtus tomentosa叶的化学成分及其抗菌活性。方法应用各种色谱技术对桃金娘叶的粗提物进行分离纯化,并利用质谱及核磁等波谱技术鉴定化合物结构;采用微量稀释法对所得化合物进行抗菌活性测试。结果从桃金娘叶95%乙醇提取物中分离得到21个化合物,分别鉴定为生育酚(1)、新生育酚(2)、生育酚-对苯醌(3)、生育酚A(4)、(-)-α-tocospirone(5)、rhodomyrtosone F(6)、rhodomyrtosone C(7)、watsonianone A(8)、rhodomyrtone(9)、2,6-二羟基-苯甲酸苯甲酯(10)、肉桂酸甲酯(11)、柚皮素(12)、槲皮素(13)、杨梅素(14)、3,7,3′-三甲氧基-5,4′,5′-三羟基黄酮(15)、5,7,3′,5′-四羟基黄酮(16)、艾纳香素(17)、7,4′-二甲氧基二氢槲皮素(18)、4′-二甲氧基二氢槲皮素(19)、2,4,7,8,9,10-六羟基-3-甲氧基蒽-6-O-β-L-吡喃鼠李糖苷(20)、4,8,9,10-四羟基-2,3,7-三甲氧基蒽-6-O-β-D-吡喃葡萄糖苷(21)。结论化合物6为新天然产物,化合物2~5,10~13及15~19为首次从该属植物中分离得到,抗菌实验结果显示化合物3及6~9具有一定的抗菌活性。     

槐花药效成分含量比较与相关性研究

正槐花系豆科植物槐Sophora japonica L.的干燥花,味苦,微寒,归肝、大肠经,具有凉血止血,清肝泻火等功效,用于便血、痔血、血痢、崩漏、吐血、衄血、肝热目赤、头痛眩晕等症~[1-3]。化学与药理学研究~[4-9]表明,槐花含有黄酮苷、脂肪酸、挥发油、氨基酸、糖类     

Anti-helicobacter pylori effect of total alkaloids of sophora alopecuroides in vivo

Background Helicobacterpylori （H.pylori） infection could lead to most gastroduodenal diseases and is even identified as a carcinogen of gastric cancer.Total alkaloids of sophora alopecuroides （TASA） is widely used in herbal remedies to treat various infectious diseases,including stomach-associated diseases.This study is aimed at evaluating the antimicrobial activity of TASA on H.pylori-infected BALB/c mice mouse gastritis.Methods Totally 120 BALB/c mice were orally inoculated with H.pylori Bacterial liquid to construct BALB/c mice H.pylori infection gastritis animal model,after the model was successfully created.We randomly assigned 100 infected mice into 10 treatment groups,the first group （normal saline）; the second group （bismuth pectin）; the third group （omeprazole）; the fourth group （TASA 2 mg/d）; the fifth group （TASA 4 mg/d）; the sixth group （TASA 5 mg/d）; the seventh group （TASA ＋ bismuth pectin）; the eighth group （TASA ＋ omeprazole）; the ninth group （bismuth pectin ＋ clarithromycin ＋ metronidazole）;the tenth group （omeprazole ＋ clarithromycin ＋ metronidazole）,5 other non-infected mice as negative control.Mice were orally inoculated twice a day and 7 days continuously.Then the mice were killed 4 weeks after treatment,we used realtime PCR to detect 16sDNA of H.pylori to test both the colonization and the clearance mice of bacteria of each treatment.We applied hematoxylin and eosin （HE） staining and immunostaining of mice gastric mucosa to observe the general inflammation and related factors interleukin 8 （IL-8）,cyclooxygenase 2 （COX-2）,and nuclear factor-kappa B （NF-KB） expression change after treatments.Results Firstly,we ensured that after 6-week intragastric administration,the bacteria colonization reached an exceed peak which is far higher than positive threshold （P ＜0.001）; secondly,after treatments,it is revealed that TASA combined with omeprazole or bismuth pectin showed promising antimicrobial activity against H.pylor     

HPLC 同时检测山豆根中苦参碱和氧化苦参碱含量简

目的建立离子交换树脂纯化．HPLC分析方法用于同时测定山豆根中苦参碱和氧化苦参碱含量。方法山豆根药材提取液经阳离子交换树脂柱纯化后,以C-18化学键合硅胶为固定相,流动相为乙腈-0．2％磷酸-三乙胺（8：92：0．01;V／V／V）,检测波长208nm。结果在本文建立的分析条件下。苦参碱和氧化苦参碱的色谱保留时间分别约为5．5min和8．1min,10min内可完成1次分离分析过程。苦参碱进样浓度在1．0～300．0μg·ml-1范围与峰面积线性关系良好（r2=0．9996）。氧化苦参碱进样量在2．0～600．0μg·ml-1范围与峰面积线性良好（r2=0．9998）。苦参碱、氧化苦参碱平均测定回收率分别为90．5％和91．6％,方法已应用于同时测定不同产地山豆根药材中苦参碱和氧化苦参碱含量。结论山豆根样品经纯化分离后,苦参碱、氧化苦参碱与药材中其余内源性物质分离完全,定量准确．方法适用于山豆根药材质量控制及其药品含量检测。     

苦参凝胶联合微波治疗宫颈糜烂的Meta分析

目的:系统评价苦参凝胶联合微波治疗宫颈糜烂的临床疗效。方法:计算机检索中国期刊全文数据库(CNKI)、中国优秀硕士/博士学位论文数据库、万方数据库、维普中文科技期刊数据库(VIP)、MEDLINE、EMBASE,查找2000年至2013年发表的有关苦参凝胶联合微波治疗宫颈糜烂的随机对照试验(RCT)。按照Cochrane系统评价方法进行筛选,采用Revman 5.2软件进行Meta分析。结果:共纳入7个研究,共计773例患者。Meta分析结果显示:苦参凝胶联合微波治疗宫颈糜烂的手术治愈率明显高于单纯采用微波治疗[OR=2.80,95%CI(1.95,4.03),P0.000 01],且采用苦参凝胶联合微波治疗患者的术后宫颈排液或出血时间较单纯采用微波治疗患者明显缩短[MD=-6.64,95%CI(-7.23,-6.04),P0.000 01]。结论:苦参凝胶联合微波治疗宫颈糜烂可明显提高患者的微波手术治愈率,显著缩短患者的术后宫颈排液或出血时间。     

Flavonoids from Sophora moorcroftiana and their Synergistic Antibacterial Effects on MRSA

Synergy is now a widely recognized approach that has direct applicability for new pharmaceuticals. The ethanolic extract of the aerial parts of the herb Sophora moorcroftiana showed significant antibacterial activity against drug‐resistant Staphylococcus aureus, and its minimum inhibitory concentration (MIC) was 8 µg/mL. In a phytochemical study of the extract, five flavonoids were obtained. However, the isolates exhibited antibacterial activity in the range of 32–128 µg/mL, which was weaker than the extract. In combination with antibiotics, the antibacterially inactive compound genistein (1) and diosmetin (4) showed significant synergistic activity against drug‐resistant S. aureus. In combination with norfloxacin, genistein (1) reduced the MIC to 16 µg/mL and showed synergy against strain SA1199B with a fractional inhibitory concentration index (FICI) of 0.38. With the antibiotics norfloxacin, streptomycin and ciprofloxacin, diosmetin (4) showed synergy against SA1199B, RN4220 and EMRSA‐15, with FICI values of 0.38, 0.38 and 0.09, respectively. In an efflux experiment to elucidate a plausible mechanism for the observed synergy, genistein showed marginal inhibition of the NorA efflux protein. Copyright © 2013 John Wiley & Sons, Ltd.     

苦参素治疗慢性荨麻疹患者疗效及外周血Foxp3+调节性T细胞的检测

8,P<0.01),而联合用药组与苦参素两组比较差异无统计学意义(t=0.529,P>0.05).注射苦参素能显著升高荨麻疹患者外周血中Foxp3+CD4+CD25+T细胞数量,苦参素各剂量组与正常人对照组及咪唑斯汀组比较差异有统计学意义(t=24.88,P<0.01).结论 苦参素对慢性荨麻疹患者疗效显著.苦参素能显著升高慢性荨麻疹患者外周血中Foxp3+CD4+CD25+T细胞数量.     

苦参总生物碱固体脂质纳米粒的制备工艺研究

目的 探索苦参总生物碱(TA)磷脂复合物固体脂质纳米粒(TA-PC-SLN)的制备工艺及处方优化,并对其进行体外评价.方法 建立高效液相色谱(HPLC)法同时测定TA中4个指标成分苦参碱(MT)、氧化苦参碱(OMT)、槐果碱(SC)、氧化槐果碱(OSC),并进行方法学验证.称取TA、卵磷脂共同溶于乙醇制备磷脂复合物;采...     

苦参半仿生提取法与水提取法的比较

目的:选择苦参药效物质较佳的提取方法。方法:以苦参碱、氧化苦参碱、苦参总碱、HPLC总面积、干浸膏为指标,综合评判,对苦参半仿生提取法与水提取法进行比较。结果:5种指标成分及综合评价Y值顺序皆为:半仿生提取法>水提取法。结论:苦参药材提取用半仿生提取法优于水提取法。