气候因素对青铜文物模拟材料大气腐蚀的影响

运用QCM反应性监测方法研究了暴露在博物馆模拟展柜中的青铜文物模拟材料的腐蚀行为及规律,并利用扫描电镜(SEM)、能谱仪(EDS)以及光电子能谱(XPS)分析手段,对青铜文物模拟材料在腐蚀60 d后的腐蚀形貌与产物进行了分析。结果表明:光照的存在以及温度、湿度的升高都会加速青铜文物模拟材料的腐蚀,其中ERCO LED灯在色温为4 000 K时对青铜文物模拟材料的腐蚀影响最严重,在腐蚀60 d后的主要腐蚀产物为Cu₂O、CuO、SnO、SnO₂,还有少量铜的碳酸盐、铜的硫酸盐与铜的硫化物、铜的硝酸盐与亚硝酸盐、铜的氯化物。     

强脉冲光治疗雀斑的临床探讨

目的:探讨强脉冲光对面部雀斑的治疗效果.方法:采用强脉冲光治疗仪波长为560nm～1 200nm滤光片治疗181例面部雀斑病变患者,治疗4次～5次,每次时间间隔为3周,每位患者进行对比拍照、记录参数并进行临床疗效评估.结果:181例雀斑患者的显效率为:99.44%(180/181).结论:强脉冲光对雀斑有良好的疗效.     

Applicability Assessment of Different Materials for Standards Ensuring Comparability of Optical and Tactile Coordinate Measurements

Multisensor CMMs are systems with an established position on the market, but their popularity still grows, as they provide access to the advantages offered by tactile and contactless measurement methods. Yet there are still questions of the comparability of results obtained using the optical and tactile operation modes of multisensor system. This phenomenon can be assessed by measuring appropriate gauges, most often reference rings or spheres. Due to the completely different nature of probing processes for tactile and contactless measurements, the material from which reference object is made may significantly affect measurement results. In order to assess the influence of this factor on measurement accuracy, three reference spheres made from different materials were measured on optical multisensor CMMs. Measurements involved tactile measurements as well as optical measurements made using different probing systems: a video probe and white light sensor. Results obtained from performed experiments show large differences depending on the material used for spherical standard production. On the basis of obtained results, it can be stated that the best material for a reference object that can be used for comparability tests of tactile and optical measurements is a composite of alumina with at least one oxidic additive.     

Targeting whole body metabolism and mitochondrial bioenergetics in the drug development for Alzheimer's disease

Aging is by far the most prominent risk factor for Alzheimer's disease (AD), and both aging and AD are associated with apparent metabolic alterations. As developing effective therapeutic interventions to treat AD is clearly in urgent need, the impact of modulating whole-body and intracellular metabolism in preclinical models and in human patients, on disease pathogenesis, have been explored. There is also an increasing awareness of differential risk and potential targeting strategies related to biological sex, microbiome, and circadian regulation. As a major part of intracellular metabolism, mitochondrial bioenergetics, mitochondrial quality-control mechanisms, and mitochondria-linked inflammatory responses have been considered for AD therapeutic interventions. This review summarizes and highlights these efforts.     

Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy

Parkin, an E3 ubiquitin ligase, plays a role in maintaining mitochondrial homeostasis through targeting damaged mitochondria for mitophagy. Accumulating evidence suggests that the acetylation modification of the key mitophagy machinery influences mitophagy level, but the underlying mechanism is poorly understood. Here, our study demonstrated that inhibition of histone deacetylase (HDAC) by treatment of HDACis activates mitophagy through mediating Parkin acetylation, leading to inhibition of cervical cancer cell proliferation. Bioinformatics analysis shows that Parkin expression is inversely correlated with HDAC2 expression in human cervical cancer, indicating the low acetylation level of Parkin. Using mass spectrometry, Parkin is identified to interact with two upstream molecules, acetylase acetyl-CoA acetyltransferase 1 (ACAT1) and deacetylase HDAC2. Under treatment of suberoylanilide hydroxamic acid (SAHA), Parkin is acetylated at lysine residues 129, 220 and 349, located in different domains of Parkin protein. In in vitro experiments, combined mutation of Parkin largely attenuate the interaction of Parkin with PTEN induced putative kinase 1 (PINK1) and the function of Parkin in mitophagy induction and tumor suppression. In tumor xenografts, the expression of mutant Parkin impairs the tumor suppressive effect of Parkin and decreases the anticancer activity of SAHA. Our results reveal an acetylation-dependent regulatory mechanism governing Parkin in mitophagy and cervical carcinogenesis, which offers a new mitophagy modulation strategy for cancer therapy.     

Free light chains as an emerging biomarker in saliva: Biological variability and comparisons with salivary IgA and steroid hormones

BackgroundSalivary free light chains (FLCs) are an emerging biomarker in health and behavioural research. However, little is known regarding biological variability of salivary FLCs and how they relate to other established salivary biomarkers. This study aimed to investigate the diurnal and day-to-day variation of salivary FLCs and their relationship with salivary IgA and steroid hormones.MethodsA total of 46 healthy adults participated in studies exploring the biological variability of FLCs. Diurnal variation was investigated by collecting saliva samples immediately upon waking, 0.5 h, 3 h, 6 h, 9 h and 14 h post-waking. Saliva samples were assessed for FLCs, IgA, cortisol and dehydroepiandrosterone (DHEA). Between-day variation in FLCs and IgA was assessed by collecting saliva samples immediately upon waking for seven consecutive days. Participants underwent a dental examination to exclude oral health as a potential confounding variable. Within and between-person day-to day variation was explored in relation to a range of different factors: awakening time, sleep, exercise, well-being and alcohol consumption.ResultsSalivary secretion rates of FLCs decreased following waking and up to 3 h post-waking and then plateaued. This same pattern was observed for IgA. DHEA was stable upon waking and higher levels were seen in the morning with significantly lower levels thereafter. Cortisol levels significantly increased 0.5 h post-waking then continued to decline across the day. FLCs were significantly correlated with IgA but not cortisol or DHEA. Both FLCs and IgA parameters showed day-to-day variability, with coefficients of variation ≥ 40%. Earlier waking time was significantly correlated with higher FLC and IgA secretion rates. Inter-person differences in saliva parameter variability were observed but the degree of variation in FLCs and IgA was related within person. Inter-person day-to-day variation appeared to be uninfluenced by lifestyle or behavioural factors.ConclusionsSaliva FLCs secretion exhibits diurnal fluctuation that mirrors IgA fluctuation. Findings strongly indicate salivary FLC secretion is orchestrated by local plasma cells. FLCs and IgA both showed notable variability day-to-day, which was similar within person and influenced by awakening time. FLCs offer a promising adjunct to IgA in the measurement of oral immune activation.     

HPLC-ELSD法同时测定知母药材中5种成分的含量

目的采用高效液相色谱-蒸发光散射检测方法(HPLC-ELSD)建立同时测定知母药材中新芒果苷、芒果苷、知母皂苷BⅡ、宝藿苷Ⅰ及知母皂苷AⅢ的含量测定方法。方法采用Agilent poroshell 120 EC-C₁₈柱,流动相采用乙腈-0.2%醋酸水系统,梯度洗脱;柱温为30℃,流速为0.7 ml/min;蒸发光散射检测器以氮气为雾化气,雾化气温度为40℃,漂移管温度为90℃,氮气体积流量为2.0 L/min;进样量为20μl。结果 5种成分均能达到基线分离,新芒果苷24.1～386μg/ml(r=0.999 3)、芒果苷23.2～371μg/ml(r=0.998 6)、知母皂苷BⅡ54.2～867.2μg/ml(r=0.995 6)、宝藿苷Ⅰ5.3～84.8μg/ml(r=0.996 8)、知母皂苷AⅢ10～160μg/ml(r=0.998 9)的浓度范围内呈现良好的线性关系。5种成分的平均加样回收率在101.8%～105.0%之间,重复性RSD小于2.4%,知母药材中上述5种成分含量分别为1.62%、0.82%、7.36%、0.07%、0.34%。结论该方法...     

CO2 点阵激光和强脉冲光治疗面部光老化的疗效比较

目的 对CO2点阵激光和强脉冲光治疗面部光老化的效果进行比较。方法 选取我院2017年 1月-2018年1月收治的面部光老化患者100例,随机分为对照组及观察组,各50例。对照组行强脉冲光治疗, 观察组行CO2点阵激光治疗,比较两组治疗效果、不良反应发生率及复发率。结果 观察组治疗总有效率为 96.00%,高于对照组的84.00%（P＜0.05）;观察组不良反应发生率为6.00%,低于对照组的20.00%（P＜0.05）; 观察组复发率为6.00%,低于对照组的22.00%（P＜0.05）。结论 与强脉冲光相比,采用点阵激光技术治疗面 部皮肤光老化,能够有效改善患者的满意程度,治疗后要注意使用抗生素避免皮肤感染,通过多种措施预防 不良反应,为治疗效果提供保证。     

2种方法治疗痤疮的疗效比较

目的观察强脉冲光联合抗生素治疗痤疮的疗效。方法125例中重度痤疮患者根据患者自愿选择治疗方式分为治疗组83例、对照组42例，治疗组强脉冲光联合局部使用抗生素治疗；对照组单纯维A酸药物治疗。治疗时间分别为各组1个疗程，治疗后观察2组的临床疗效情况。结果治疗组有效率高于对照组，差异有统计学意义（P〈0．05）。结论强脉冲光联合局部使用抗生素治疗痤疮有较好的临床疗效。     

血清Kappa与Lambda轻链的检测在恶性肿瘤中的表达

目的探讨血清中Kappa和Lambda轻链在恶性肿瘤患者中的表达情况。方法采用免疫比浊法对80例恶性肿瘤患者和20例健康体检者血清中Kappa和Lambda轻链进行检测。结果肝癌组患者血清中κ和λ值均显著高于健康对照组,且差异有统计学意义(P0.01);而κ/λ比值与健康对照组比较,差异无统计学意义(P0.05)。胃癌、肺癌、乳腺癌、食道癌、大肠癌组患者血清中κ、λ、κ/λ比值分别与健康对照组比较,差异均无统计学意义(P0.05)。结论血清中Kappa和Lambda轻链的测定,可作为原发性肝癌的诊断指标和对患者免疫功能的监测。