观音合剂治疗小儿反复呼吸道感染肺脾气虚证的临床研究

目的 观察观音合剂治疗小儿反复呼吸道感染（肺脾气虚证）的临床疗效及其对分泌型免疫球蛋白A（SIgA）的影响,从而初步探讨其作用机制.方法 将80例反复呼吸道感染（肺脾气虚型）患儿随机分为两组,每组各40例,观察组服用观音合剂,对照组服用脾氨肽,疗程1个月,随访6个月.观察各组治疗前后的发病情况及中医证候积分的变化;于用药前、后6个月测定唾液SIgA含量.结果 两组疾病疗效比较,观察组总有效率为85.0％,对照组为65.0％,差异有统计学意义（P＜0.05）;两组中医证候疗效比较,观察组总有效率为90.0％,对照组为62.5％,差异有统计学意义（P＜0.05）;治疗后两组唾液SIgA含量均较治疗前升高（P＜0.01）,且观察组升高更为显著（P＜0.01）.结论 观音合剂能减少反复呼吸道感染（肺脾气虚证）患儿呼吸道感染的次数,改善中医证候,初步推测其作用机制可能与提高患儿SIgA水平有关.     

小儿急性支气管肺炎唾液SIgA及血清IgA测定

采用放射免疫法测定３０例急性支气管肺炎患儿的唾液ＳＩｇＡ，结果：患儿组唾液ＳＩｇＡ明显低于对照组（７６．９±４２．９ｍｇ／Ｌ，３２０±３２．１ｍｇ／Ｌ，Ｐ＜０．０１），同时发现患儿唾液ＳＩｇＡ与血清ＩｇＡ之间呈正相关关系（ｒ＝０．９３，Ｐ＜０．０１）。提示急性支气管肺炎患儿大多有体液免疫紊乱，以ＳＩｇＡ及ＩｇＡ降低明显。     

宫颈分泌型免疫球蛋白A对慢性盆腔炎的诊断价值

目的：探讨测定宫颈分泌物中，分泌型免疫球蛋白Ａ（ＳＩｇＡ）含量对慢性盆腔炎的诊断价值。方法：抽取宫颈管内粘液作ＳＩｇＡ定量测定，分别对９８例慢性盆腔炎，７６例治愈病例和４６０例正常妇女作测定。结果：盆腔炎组９８例病人测定结果ｘ＝１６６．８ｍｇ／Ｌ，是正常妇女的１０例，治愈组测定７６例，ｘ＝１１．６７ｍｇ／Ｌ属正常范围，正常妇女４６０例测定结果ｘ＝９．８ｍｇ／Ｌ，结论：宫颈分泌物中ＳＩｇＡ的测定，可     

机械通气患者胃液SIgA含量、pH值与胃内细菌定植的关系

目的　探讨机械通气患者胃液SIgA含量和 pH值与胃内细菌定植的关系。 方法　用 pH精密试纸测定 4 5例机械通气患者胃液pH值每日至少 1次 ,并留取胃液做细菌培养隔日 1次 ,直到患者可疑发生呼吸机相关性肺炎、脱机或死亡 ;在患者使用呼吸机 2 4h内留取胃液测定SIgA含量 ;比较胃内细菌定植者和胃内无细菌定植者胃液pH值和胃液SIgA的差异。 结果　胃内细菌定植者胃液pH值为 4 .5 5± 0 .78,显著高于胃内无细菌定植者2 .82± 1.0 9(P <0 .0 0 1) ;胃内细菌定植者胃液SIgA含量为8.81± 8.74 μg/mL,显著低于胃内无细菌定植者 90 .3±10 0 .2 μg/mL(P =0 .0 0 5 )。结论　机械通气患者胃液pH值增高和SIgA含量降低是胃内细菌定植的潜在危险因素。     

轮状病毒肠炎和鼠伤寒杆菌肠炎患儿粪便中SIgA含量测定

用放射免疫法(双抗体—PEG法)测定正常婴幼儿和轮状病毒肠炎及鼠伤菌肠炎各40例患儿粪便中SIgA的含量。结果表明轮状病毒肠炎及鼠伤寒杆菌肠炎之间粪便中SIgA含量无明显差异,而二者粪便中SIgA含量明显高于正常对照组。提示肠道SIgA在婴幼儿轮状病毒肠炎及鼠伤寒杆菌肠炎恢复中起重要作用。     

The relationship between the levels of SigA,lactoferrin and alpha(1) proteinase inhibitor in saliva and permanent dentition caries in 15-year-olds

A group of 15-year-olds were clinically examined for general state of dentition and level of dental caries. Unstimulated whole saliva from the subjects was laboratory tested to determine the levels of lactoferrin, Secretory IgA (SIgA) and alpha1 proteinase inhibitor. A significant relationship was found between the decayed surface index and the levels of lactoferrin, SIgA and alpha1 proteinase inhibitor in the unstimulated saliva. The decayed surface index rose with increases in the levels of lactoferrin, SIgA and alpha1 proteinase inhibitor, the relationship with alpha1 proteinase inhibitor being strongest.     

Faecal SIgA secretion in infants fed on pre- or probiotic infant formula

Secretory immunoglobulin A (SIgA) plays an important role in the defence of the gastrointestinal tract. The level of faecal SIgA antibody is associated with increased neutralization and clearance of viruses. Formula-fed infants who lack the transfer of protective maternal SIgA from breast milk may benefit from strategies to support maturation of humoral immunity and endogenous production of SIgA. We aimed at studying the effects of standard, prebiotic and probiotic infant formulas on the faecal SIgA levels. At birth, infants of whom the mother had decided not to breastfeed were allocated to one of three formula groups in a randomized, double-blind fashion. Nineteen infants received standard infant formula; 19 received prebiotic formula containing a specific mixture of 0.6 g galacto-oligosaccharides (GOS)/fructo-oligosaccharides (FOS)/100 ml formula and 19 received probiotic formula containing 6.0 × 10⁹ cfu Bifidobacterium animalis /100 ml formula. Faecal samples were taken on postnatal day 5, day 10, wk 4 and every 4 wk thereafter until wk 32. SIgA in faeces was determined by an enzyme-linked immunosorbent assay. During the intervention, infants fed on prebiotic formula showed a trend towards higher faecal SIgA levels compared with the standard formula-fed infants reaching statistical significance at the age of 16 wk. In contrast, infants fed on the probiotic formula showed a highly variable faecal SIgA concentration with no statistically significant differences compared with the standard formula group. Formula-fed infants may benefit from infant formulas containing a prebiotic mixture of GOS and FOS because of the observed clear tendency to increase faecal SIgA secretion. Adding viable B. animalis strain Bb-12 to infant formula did not reveal any sign for such a trend.     

非癌性胃疾病粘膜组织分泌性免疫球蛋白A体外合成与分泌的观察

应用组织培养技术对非癌性胃疾病粘膜组织分泌性免疫球蛋白A(SIgA)体外合成分泌水平进行测定,发现慢性浅表性胃炎组(4.35±0.32μg/mg)和慢性萎缩性胃炎组(4.82±0.22μg/mg)SIgA分泌量均高于正常组(2.76±0.21μg/mg),P<0.01。正常组胃窦粘膜SIgA(3.31±0.32μg/mg)高于胃体分泌量(2.31±0.29μg/mg),P<0.05。慢性浅表性胃炎与萎缩性胃炎组间及其胃窦与胃体之间SIgA分泌量无明显差异。慢性胃炎患者SIgA体外合成分泌量增高提示胃粘膜炎症促使SIgA排泄增多,以加强胃粘膜的免疫保护作用。     

双歧杆菌活菌对肠道菌群及SIgA含量的影响

给３０名健康成人、３０名儿童、１５名血液病患儿、１５名新生儿住院患者服用自行配制的含双歧杆菌的活菌饮料，分析服用前后肠道菌群及ＳＩｇＡ含量，结果提示服用外源性双歧杆菌后，健康成人、儿童、新生儿住院患者肠道内类杆菌数量减少，成人粪便中分泌ＳＩｇＡ的水平上升，儿童肠道内双歧杆菌数量增加，血液病患儿因口服抗生素，肠道菌群及ＳＩｇＡ变化不显著。     

口服微生态制剂对直肠癌患者术后肠道菌群分布及SIgA的影响

目的探讨低位直肠癌患者行腹、会阴联合直肠癌根治术(Miles术)术后口服微生态制剂对肠道菌群及粪便分泌型免疫球蛋A(SigA)含量的影响。方法将126例Miles术后患者采用随机化原则分成观察组和对照组,每组各63例。观察组患者术后给予口服双歧三联活菌胶囊(培菲康胶囊),对照组常规治疗。分别于入院时、术后第一次排便、术后1个月收集两组患者的新鲜大便,比较两组患者的肠道菌群的改变及粪便SIgA含量。结果术后第一次排便及术后1月观察组双歧杆菌、乳酸杆菌、拟杆菌数量显著高于对照组(P0.05),而肠球菌、肠杆菌数量均显著低于对照组(P0.05)。术后1个月,观察组患者的肠道菌群与手术前相比,基本恢复(P0.05)。术前及术后第一次排便,两组患者粪便中的SIgA水平无显著差异(P0.05);而术后1个月,观察组粪便SIgA含量均显著高于对照组(P0.05),且恢复至术前水平,而对照组仍低于术前水平。结论直肠癌Miles手术后口服微生态制剂可有效纠正患者肠道菌群失调,并通过升高粪便SIgA含量恢复肠道屏障功能和局部免疫力。