春季急性上呼吸道感染中医证型与sIgA相关性初步研究

目的:分析春季急性上呼吸道感染中医证型与呼吸道局部免疫分子SIgA之间的相关性。方法:选取时令病邪致病较典型的春季急性上呼吸道感染患者63例为研究对象,其中风寒型32例,风热型31例,并与30例健康人作对照,检测口咽部免疫分子SIgA状况。结果:疾病组SIgA含量较正常组显著降低(P<0.05),风热组患者SIgA含量较正常组显著降低(P<0.01),风寒组与正常组比较无显著差异。结论:春季急性上呼吸道感染患者中医证型与局部免疫分子SIgA之间存在一定相关性。     

贵阳地区不同污染环境中儿童唾液SIgA,溶菌酶含量的比较

为了解贵阳地区不同居住环境儿童的免疫功能状况,分别收集工业污染区92 例、商业交通污染区90 例、非污染区83 例儿童唾液,经平板打孔法、ELISA、单向琼脂扩散试验测定唾液溶菌酶及SIgA 含量。实验结果显示:工业污染区和商业交通污染区儿童唾液溶菌酶和SIgA 含量低于非污染区儿童,经统计学处理具有显著性差异( P< 0-05) ,证明环境污染可造成儿童免疫能力降低     

大黄对实验性大鼠坏死性胰腺炎肠道屏障保护作用

目的：观察大黄对急性坏死性胰腺炎（ANP）肠道机械屏障和免疫屏障的改变的影响，以生长押素组做对照。方法：用经胰胆管逆行注入牛磺胆酸钠复制Wistar大鼠ANP模型，观察ANP时，两种药物治疗后肠黏腱和胰腺组织结构变化，测定肠内容物中分泌型免疫球蛋白（SIgA），肠组织丙二醛（MDA），血浆淀粉酶（AMY）。结果：ANP时肠黏膜和胰腺发生病理损伤，肠内容物SIgA含量显著下降（P〈0．01）。肠组织中MDA，血中AMY含量显著升高（均为P〈0．01），且MDA与SIgA里显著负相关（r=-0．8818，r=-0．9236。P〈0．01）。大黄可降低肠组织MDA（P〈0．01），提高肠内容物SigA含量（P〈O．01）。结论：大黄和生长押素可减轻肠黏膜和胰腺组织损伤。提高肠内容物中SIgA含量。     

无龋和患龋儿童唾液中免疫组分的测定及意义

目的:探讨龋病与唾液中免疫成分的关系。方法:对130例4～6岁幼儿园儿童的患龋情况以及混合唾液的pH值、总蛋白、SIgA和溶菌酶的含量进行检查测定,并对无龋儿童和患龋儿童唾液成分进行比较。结果:130例儿童中,无龋45例,患龋85例,患龋率65.4%。患龋儿童唾液pH、总蛋白、SIgA和溶菌酶分别是(7.10±0.38)g/L、(1.68±0.40)g/L、(60.21±39.23)μg/ml和(19.33±21.20)μg/ml;无龋儿童唾液pH、总蛋白、SIgA和溶菌酶分别是(7.16±0.41)g/L、(1.46±0.35)g/L、(49.31±28.13)μg/ml和(15.43±13.25)μg/ml。无龋儿童和患龋儿童唾液pH值差异无显著性,患龋儿童唾液总蛋白、SIgA及溶菌酶含量明显高于无龋儿童,两者比较差异有显著性(P<0.05)。结论:唾液总蛋白、SIgA及溶菌酶在龋病发病中可能起重要作用。     

黄芪注射液穴位注射对慢性盆腔炎大鼠局部SIgA及病理形态学改变的影响

目的：观察黄芪注射液穴位注射对慢性盆腔炎大鼠模型局部分泌型IgA(SIgA)及病理形态学改变的影响。方法：50只Wistar大鼠随机分成6组,除正常组和假手术组外,其余造成慢性盆腔炎模型；黄芪注射液穴注组和生理盐水穴注组选用关元、足三里进行穴位注射；阳性药对照组用妇科千金片灌胃； 模型组、正常组和假手术组常规饲养,不予处理。观察各组大鼠子宫的组织形态学变化,检测阴道冲洗液中SIgA的含量。结果：模型组病理形态学检查显示慢性炎症改变,黄芪组和妇科千金片组改变不明显；黄芪组局部SIgA含量显著高于其他5组,模型组SIgA含量最低。结论：慢性盆腔炎反复发作与局部SIgA分泌不足、局部免疫力下降有关；黄芪针穴位注射治疗慢性盆腔炎可以刺激局部黏膜分泌SIgA,提高黏膜的局部免疫功能,防止炎症复发。     

大黄对实验性大鼠坏死性胰腺炎肠道屏障保护作用

目的:观察大黄对急性坏死性胰腺炎(ANP)肠道机械屏障和免疫屏障的改变的影响,以生长抑素组做对照。方法:用经胰胆管逆行注入牛磺胆酸钠复制W istar大鼠ANP模型,观察ANP时,两种药物治疗后肠黏膜和胰腺组织结构变化,测定肠内容物中分泌型免疫球蛋白(SIgA),肠组织丙二醛(MDA),血浆淀粉酶(AMY)。结果:ANP时肠黏膜和胰腺发生病理损伤,肠内容物SIgA含量显著下降(P<0.01),肠组织中MDA,血中AMY含量显著升高(均为P<0.01),且MDA与SIgA呈显著负相关(r=-0.8818,r=-0.9236,P<0.01)。大黄可降低肠组织MDA(P<0.01),提高肠内容物S igA含量(P<0.01)。结论:大黄和生长抑素可减轻肠黏膜和胰腺组织损伤,提高肠内容物中SIgA含量。     

Effects of lipopolysaccharide on the histomorphology and expression of toll-like receptor 4 in the chicken trachea and lung

Endotoxin or lipopolysaccharide (LPS) exposure can cause injury to the respiratory airways and in response, the respiratory epithelia express toll-like receptors (TLRs) in many species. However, its role in the innate immunity in the avian respiratory system is poorly understood. The aim of the present study was to evaluate the effects of LPS on the chicken trachea and lung. After intraperitoneal LPS or saline injection, the trachea and lungs were harvested at 0, 12, 36 and 72?h (n?=?6 at each time point) and histopathologically analysed using haematoxylin and eosin and periodic acid-Schiff staining, while TLR4 expression was determined by immunohistochemistry and secretory Immunoglobulin A (SIgA) levels by enzyme-linked immunosorbent assay. After LPS stimulation, we observed a remarkable decrease in the number of goblet cells along with obvious disruption and desquamation of the ciliated epithelium in the trachea, blurring of the boundary between pulmonary lobules, narrowed or indistinguishable lumen of the pulmonary atria and leukostasis in the lungs. Following LPS stimulation, TLR4 protein expression was up-regulated in both the trachea and the lungs and was found on the ciliated columnar cells as well as in the submucosa of the trachea, and in the lungs on parenchymal and immune cells. However, SIgA levels were only up-regulated in the trachea at 12?h following LPS stimulation. Hence, this report provides novel information about the effects of LPS on the microstructure of the lower respiratory tract and it is concluded that its intra-peritoneal administration leads to TLR4-mediated destruction of the tracheal epithelium and pulmonary inflammation along with increased SIgA expression in the tracheal mucosa.     

The effects of Lactobacillus pentosus strain b240 and appropriate physical training on salivary secretory IgA levels in elderly adults with low physical fitness: a randomized,double-blind,placebo-controlled trial

The purpose of the study was to evaluate the effects of Lactobacillus pentosus strain b240 (b240) intake and appropriate physical training on salivary secretory immunoglobulin A secretion in elderly adults with low physical fitness. Elderly adults with low physical fitness (daily step count below 3,500 steps) were divided into 2 groups: a b240 intake + exercise group (b240 group) and a placebo intake + exercise group (placebo group). Each subject continued intake of b240 or placebo and moderate-intensity resistance exercise for 12 weeks. Before and 4, 8, and 12 weeks after the start of intervention, each subject underwent saliva sampling. Before and after intervention, physical fitness tests and step count were measured. Our results showed that secretory immunoglobulin A secretion in 57 subjects during the b240/placebo intake period was significantly greater in the b240 group than in the placebo group (p<0.05). There were no significant changes in physical fitness tests before and after intervention in the 2 groups. The daily amount of walking increased significantly after intervention in both groups (p<0.05). These results suggest that in elderly adults with low physical activity and fitness, intake of b240 with appropriate physical exercise elevate salivary secretory immunoglobulin A secretion.     

清喉利咽颗粒联合克拉霉素治疗慢性咽炎的临床研究

目的探讨清喉利咽颗粒联合克拉霉素缓释片治疗慢性咽炎的临床疗效。方法选取2017年6月-2019年3月在天津医科大学中新生态城医院治疗的176例慢性咽炎患者,根据用药差别分为对照组(88例)和治疗组(88例)。对照组口服克拉霉素缓释片,0.5 g/次,1次/d;治疗组在对照组基础上口服清喉利咽颗粒,5 g/次,3次/d。两组患者均经1周治疗。观察两组患者临床疗效,同时比较治疗前后两组患者临床症状积分、血清C反应蛋白(CRP)、白细胞介素-1β(IL-1β)、血管内皮细胞黏附分子1(VCAM-1)、表皮生长因子(EGF)、白细胞介素-2(IL-2)和分泌型免疫球蛋白A(SIg A)水平。结果治疗后,对照组临床有效率为81.82%,显著低于治疗组的97.73%,两组比较差异有统计学意义(P<0.05)。经治疗,两组患者临床症状积分均显著降低(P<0.05),且治疗组积分明显低于对照组(P<0.05)。经治疗,两组患者血清CRP、IL-1β和VCAM-1水平明显降低(P<0.05),血清EGF、IL-2、SIg A水平明显升高(P<0.05),且治疗组这些血清细胞因子水平明显好于对照组(P<0.05)。结论清喉利咽颗粒联合克拉霉素缓释片治疗慢性咽炎可有效改善患者临床症状,降低机体炎症反应,具有一定的临床推广应用价值。     

Salivary anti-RSV IgA antibodies and respiratory infections during the first year of life in atopic and non-atopic infants

Salivary SIgA antibodies against RS virus were studied in 105 children during the first year of life. The infants were divided into groups according to their risk of atopy. At birth 13 neonates showed measurable amounts of SIgA to RS virus. In another 26 children specific antibodies were detected but in concentrations too low for quantitative analysis. During the first year of life this increased to 29 antibody-positive samples with measurable amounts of antibody and 39 with concentrations too low for quantitative determination. At this time 8 children of the high risk group had developed symptoms of allergy. None of these children had measurable amounts of SIgA anti-RSV in their saliva. In comparison, 10 of the remaining 26 high risk infants without symptoms of allergy did have such antibodies. Atopic infants had significantly more respiratory infections during the first year of life than nonatopic infants. The avidity of SIgA anti-RSV in neonatal samples was significantly higher than avidity determined in breast milk SIgA but comparable to the avidity of serum IgG. During the first year of life a continuing decrease of salivary SIgA avidity was observed.