肥胖人群脂联素、抵抗素和胰岛素抵抗水平的研究

目的 探讨肥胖患者血清脂联素、抵抗素和胰岛素抵抗水平的变化及意义.方法 分别测定106例肥胖者和107名健康对照者的体重指数(BMI)、腰围、臀围、空腹胰岛素、空腹血糖、血脂、脂联素和抵抗素水平,计算胰岛素抵抗指数(HOMA-IRI). 结果 肥胖者的体重指数(BMI)、体脂分布百分比(BF)、HOMA-IRI、抵抗素...     

颅脑外伤合并SIRS患者血清抵抗素与C-反应蛋白的动态变化

目的通过监测颅脑外伤合并全身炎症反应综合征(SIRS)患者血清抵抗素与C-反应蛋白(CRP)的动态变化,探讨其在观察颅脑外伤患者病情变化中的价值。方法 78例颅脑外伤患者分为两组,SIRS组(颅脑外伤合并SIRS)37例和非SIRS组(颅脑外伤不合并SIRS)41例,分别于伤后24h内、第3天、第5天、第7天上午8时抽取静脉血,分离血清后抵抗素采用酶联免疫吸附双抗体夹心方法测定,CRP采用免疫速率散射比浊法检测二者水平。结果两组颅脑损伤患者伤后24h血清抵抗素、CRP含量均显著高于对照组(P均0.01)。非SIRS组患者血清抵抗素、CRP水平在第3天开始下降。SIRS组患者血清抵抗素和CRP水平虽经治疗仍持续上升,两组上述指标同时间比较,差异有统计学意义(P0.01或P0.05),两组患者血清抵抗素与CRP呈正相关(r=0.689,P0.01)。结论抵抗素和CRP水平可以用于评估颅脑外伤是否合并SIRS,对于监测SIRS的发生具有较好的临床意义,并有助于准确及时地评估病情和判断预后。     

Resistin as a putative modulator of insulin action in the daily feeding/fasting rhythm

Resistin and adiponectin are adipokines with postulated opposite functions. Resistin has been related with insulin resistance in obesity, while adiponectin could be associated to higher insulin sensitivity. We have determined whether the production of these two adipokines during the day is related to the feeding rhythm in rats. Resistin mRNA levels in adipose tissue correlated positively with the gastric contents and serum insulin concentration, showing higher levels during the dark phase (period of the highest food intake), especially in the mesenteric depot, while levels decreased during the light phase. The diurnal pattern of resistin expression was not directly reflected in the circulating levels, but it showed a 6-h delay and correlated negatively with the gastric contents and serum insulin. Adiponectin expression followed an opposite pattern, not apparently related to feeding or insulin release, and not translated into changes in circulating levels. Moreover, considering that insulin stimulates resistin expression and that circulating resistin follows a contrary circadian pattern in comparison to insulin, resistin, apart from its role in the increased insulin resistance associated to obesity, could also act as a putative modulator of insulin in the daily feeding/fasting rhythm through a negative feedback regulation of its action.     

Decreased levels of plasma adiponectin associated with increased risk of colorectal cancer

AIM:To investigate the association between adiponectin levels and risk of colorectal adenoma and cancer (early and advanced).METHODS: A cross-sectional study in a cohort of hospital-based patients was conducted between January 2004 and March 2006 at Yamagata University Hospital. Male subjects, who had colorectal tumors detected by endoscopic examination, were enrolled according to inclusion and exclusion criteria. Based on the T factor of the TNM system, intraepithelial carcinoma and submucosally invasive c...     

2型糖尿病肾病与血浆抵抗素及胰岛素抵抗的关系

目的探讨血浆抵抗素及胰岛素抵抗与2型糖尿病肾病(DN)之间的相互关系。方法选取60例临床确诊的2型糖尿病患者,按有无DN分为2组:DN组30例和无DN组30例,以30例同期体检的健康志愿者作为对照组。采用双抗体夹心酶联免疫吸附试验测定所选90例实验对象的血浆抵抗素浓度,时间分辨分析法测定胰岛素,氧化酶法测定血糖,全自动生化分析仪检测血脂。结果①DN组血浆抵抗素浓度高于无DN组及对照组(P0.05),差异有统计学意义;②60例病例相关分析得出:血浆抵抗素浓度与空腹胰岛素(FINS)、糖化血红蛋白(HbAlc)、胰岛素抵抗指数(HOMA-IR)、胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)呈正相关,与空腹血糖(FPG)、甘油三酯(TG)、体重指数(BMI)、高密度酯蛋白胆固醇(HDL-C)均无相关性。结论空腹血浆抵抗素水平在2型糖尿病有DN者中显著升高,并与2型糖尿病患者血糖控制不良的程度有关、与2型糖尿病脂代谢紊乱相关,在2型DN的发生、发展中可能起一定作用。     

Obesity and colorectal cancer:Role of adipokines in tumor initiation and progression

Obesity-associated diseases account for a large portion of public health challenges.Among obesity-related disorders,a direct and independent relationship has been ascertained for colorectal cancer(CRC).The evidence that adipocyte hypertrophy and excessive adipose tissue accumulation(mainly visceral)can promote pathogenic adipocyte and adipose tissue-related diseases,has led to formulate the concept of"adiposopathy",defined as adipocyte and adipose tissue dysfunction that contributes to metabolic syndrome.Adipose tissue can,indeed,be regarded as an important and highly active player of the innate immune response,in which cytokine/adipokine secretion is responsible for a paracrine loop between adipocytes and macrophages,thus contributing to the systemic chronic low-grade inflammation associated with visceral obesity,which represents a favorable niche for tumor development.The adipocyte itself participates as a central mediator of this inflammatory response in obese individuals by secreting hormones,growth factors and proinflammatory cytokines,which are of particular relevance for the pathogenesis of CRC.Among adipocyte-secreted hormones,the most relevant to colorectal tumorigenesis are adiponectin,leptin,resistin and ghrelin.All these molecules have been involved in cell growth and proliferation,as well as tumor angiogenesis and it has been demonstrated that their expression changes from normal colonic mucosa to adenoma and adenocarcinoma,suggesting their involvement in multistep colorectal carcinogenesis.These findings have led to the hypothesis that an unfavorable adipokine profile,with a reduction of those with an anti-inflammatory and anti-cancerous activity,might serve as a prognostic factor in CRC patients and that adipokines or their analogues/antagonists might become useful agents in the management or chemoprevention of CRC.     

川崎病患儿治疗前后血清抵抗素和内脂素的变化

目的 检测急性期川崎病（KD）患儿静脉注射丙种球蛋白（IVIG）治疗前后血清中抵抗素和内脂素含量的变化及意义。方法 选择2011年1月至2013年1月确诊的KD患儿50例为研究对象,同时选取30例健康儿童和30例急性感染性疾病患儿作为对照。酶联免疫吸附法检测KD患儿IVIG治疗前后及对照儿童血清中抵抗素和内脂素的水平。结果 KD患儿血清抵抗素和内脂素含量均明显高于健康对照组和急性感染性疾病患儿（均P<0.05）;经过48 h治疗后,IVIG治疗有效KD患儿血清抵抗素含量较治疗前明显降低（P<0.05）,内脂素含量在IVIG治疗有效KD患儿治疗前后差异无统计学意义（P >0.05）;IVIG治疗无效KD患儿（n=12）治疗前血清抵抗素水平明显高于IVIG治疗有效组（n=38,P<0.05）,而内脂素含量在两组患儿治疗前差异无统计学意义（P >0.05）;KD合并冠脉损害与非冠脉损害患儿的抵抗素和内脂素水平差异均无统计学意义（P >0.05）。结论 KD患儿血清中高表达的抵抗素和内脂素可能参与了KD的发生和发展;血清抵抗素含量可能成为临床观察IVIG治疗效果的新监测指标。     

Resistin expression in breast cancer tissue as a marker of prognosis and hormone therapy stratification

Objective

Adipocytokines are adipocyte-derived hormones and well documented to be involved in carcinogenesis. The expression of resistin, a newly discovered adipocytokine, in breast cancer tissues was determined and correlated with patient clinicopathological variables.

Methods

Resistin expression in breast cancer tissues and the normal adjacent breast tissues was analyzed by immunohistochemistry and was correlated with clinicopathological variables as well as recurrence rates by the chi-square test. The prognostic value of resistin for disease-free and overall survival was determined by Kaplan-Meier estimates, and the significance of differences between curves was evaluated by the log-rank test.

Results

High resistin expression was predominantly observed in breast cancer tissues but not the adjacent normal breast tissues. High resistin expression in breast cancer tissues was correlated significantly with tumor stage, tumor size, lymph node metastasis and estrogen receptor status. Hormone therapy, but not radiotherapy or chemotherapy, decreased the recurrence rate in patients with high resistin expression. While high resistin expression was associated with poor disease-free and overall survival, Cox regression analysis also revealed that resistin was an independent predictor of disease-free and overall survival.

Conclusions

High resistin expression in breast cancer tissue is associated with a more malignant clinicopathological status as well as poor patient survival. Resistin may therefore hold promise as an independent prognosis predictor for breast cancer, as a marker for hormone therapy stratification and as a potential therapeutic target.     

Adipokines,cytokines and body fat stores in hepatitis C virus liver steatosis

AIM: To identify patients with or without liver steatosis and its severity in treatment-na?ve patients affected by hepatitis C virus(HCV) infection.METHODS: We included 56 HCV infected patients, and assessed the amount of liver fat by histomorphometry, and its relationships with fat and lean mass at different parts of the body(by densitometry), hormones [insulin, homeostatic model assessment(HOMA)], adipokines(resistin, adiponectin, leptin), and cytokines(tumor necrosis factor α, interleukin-6).RESULTS: Although the intensity of liver steatosis is related to trunk fat mass and HOMA, 33% of patients showed no liver steatosis, and this finding was not related to body mass index or genotype. Besides trunkfat mass, no other factor was related to the presence or not of liver steatosis, or to the intensity of it, by multivariate analysis. Lean mass was not related to liver steatosis. Adiponectin levels were lower among patients. No differences were observed in leptin and resistin.CONCLUSION: Steatosis in HCV infection is common(67.2%), and closely related to trunk fat, and insulin resistance, but not with leg fat mass or adipokines.