清痹片的质量标准研究

[目的]建立清痹片的质量控制标准。[方法]对处方中的全蝎进行显微鉴别;采用石油醚 (60～90℃) -乙酸乙酯 (1:1)为展开剂,以靛蓝、靛玉红为对照品,对处方中的青黛进行薄层鉴别。[结果]显微镜下观察到全蝎中刚毛和横纹肌纤维的显微特征;薄层色谱鉴别中,供试品 (清痹片)色谱与对照品色谱显相同颜色的斑点。[结论]该方法简单易行,重现性好,可以做为清痹片的质量控制标准。     

神乐片中钩藤碱的双波长薄层扫描定量法

采用双波长薄层扫描法测定了神乐片中钩藤碱的含量。钩藤碱的最大吸收波长是２５３ｎｍ，参比波长是３７０ｎｍ。本法操作简便，结果较好，为控制产品质量提供了科学依据。     

猪牙皂的薄层色谱鉴别研究

目的:确定猪牙皂薄层色谱鉴别的实验条件.方法:采用3种不同的展开剂分别进行猪牙皂薄层色谱鉴别研究.结果:猪牙皂薄层色谱条件为:采用硅胶G板,以甲苯-醋酸乙酯-甲酸(14∶4∶0.5)为展开剂展开,10%硫酸乙醇溶液为显色剂,在105℃加热至斑点显色清晰.结论:本实验精密度好且操作简便易行.     

Some binding properties of Omp T digested muscle tropomyosin

Cleavage of vertebrate muscle tropomyosin by bacterial Omp T produces an amino-terminally truncated product (residues 7–284). The proteolysed protein, which is resolved from the parent by electrophoresis in the presence of sodium dodecylsulphate, can be generated from a variety of striated and smooth muscle tropomyosins, including ones from mammal, bird and fish. Edman-based sequencing and mass analysis confirm that the main site of chain hydrolysis is the peptide bond between Lys 6 and Lys 7. Loss of the hexapeptide, together with the blocking group, from tropomyosin weakens its affinity for troponin. Compared to wild type, the shortened forms of rabbit skeletal tropomyosin and Atlantic salmon fast skeletal tropomyosin, as well as the unacetylated (full-length) version of the latter, all display reduced affinity for both troponin and the amino-terminal fragment of troponin-T (residues 1–158), as judged by affinity chromatography. This is consistent with the view that the amino terminal region is required for full interaction with troponin-T. Truncated tropomyosin fails to bind to F-actin at micromolar concentration, as expected. Interestingly, binding is restored by troponin in the presence of either added Ca²⁺ or EGTA. Digestion of muscle tropomyosin by Omp T, which can be carried out on quantitative amounts of protein, is concluded to yield a product that has useful biochemical applications.     

Liquid-based endometrial cytology in the management of sonographically thickened endometrium

Liquid-based cytology represents an opportunity to re-evaluate endometrial cytology. We evaluated the accuracy of liquid-based endometrial cytology as compared to biopsy in 670 women scheduled for histeroscopy because of thickened endometrium (>4 mm), as evaluated by transvaginal sonography. Endometrial biopsy detected pathology in 41 (6%) of cases (21 of which were adenocarcinomas). Cytologic study found pathology in 62 (9%) cases (19 of which were adenocarcinomas). Two hundred ninety-one biopsies (43%) and 28 (4%) cytologies were inadequate. The sensitivity and the specificity were estimated, respectively, at 95% and 98%; the positive and negative predictive values were estimated, respectively, at 83% and 99%. Cytology provided sufficient material more often than biopsy (P < 0.01). We consider endometrial cytology an efficacious diagnostic opportunity. It could be usefully applied in association with transvaginal sonography. The combination of these procedures might reduce more invasive and expensive diagnostic procedures.     

Violet Emission of ALD‐Grown ZnO Nanostructures on Confined Polymer Films: Defect Origins and Emission Control via Interface Engineering Based on Confinement of the Bottom Polymer Template

ZnO thin films on polymer templates (polystyrene, PST) show strong influence of the interface via degree of confinement of the bottom polymer template in introducing the controlled defect levels in the ZnO nanostructures and consequently yield significant variation at room temperature photoluminescence (PL). The thickness of the polymer thin film in terms of its radius of gyration (R _g) defines the degree of confinement of PST. Simultaneous growth of ZnO thin films by atomic layer deposition (ALD) with 150 cycles at 35 °C on spin‐coated polystyrene (PS) films of different thicknesses forms sets of ZnO/PST hybrid systems. The ZnO thin film exhibits a distinct/unusual violet emission (2.95 eV) along with the common near‐band‐edge and green emissions, when it grows on 6.5R _g PST. Such defect‐induced emissions disappear with increasing degree of confinement of the bottom PST from 6.5R _g to 1.5R _g, and sharper UV peak dominates the PL spectra along with a prominent redshift in near‐band‐edge. Furthermore, with increasing film thickness of the bottom PST template from 1.5R _g to 6.5R _g, the ALD growth rate of ZnO increases by 25%. The study offers new insights into the research on opto‐electronic applications of ZnO thin films in the emerging field of organic electronics.     

腹部超薄管状皮瓣修复术治疗手指脱套伤的疗效观察

目的：探讨腹部超薄管状皮瓣修复术（Abdominal thin tubular flap repair,ATTFR）治疗手指脱套伤的临床疗效。方法选择86例该院收治的手指脱套伤患者,按照数字表法随机分为两组,对照组43例采用腹部袋状皮瓣修复术（Abdominal pocket flap repair,APFR）治疗;观察组43例采用腹部超薄管状皮瓣修复术（ATTFR）治疗,并观察两组术后1d及术后1、6个月手部整体功能、掌指关节、近位指间关节活动度,治疗效果与术后并发症。结果观察组的总有效率为95.35%,明显高于对照组的79.07%（χ2=5.1079,P<0.05）,两组的手部整体功能评级随着术后时间延长均而显著地提高,掌指关节活动度与近位指间关节活动度随着时间延长均显著增大（P<0.05）,并发症发生率显著低于对照组（χ2=4.9143,P<0.05）。结论腹部超薄管状皮瓣修复术治疗手指脱套伤疗效肯定可靠,能够快速恢复患者术后手部整体功能、掌指关节及近位指间关节活动度,减少并发症,值得临床大力推广应用。     

普通CT2mm薄层骨算法扫描在肺部病变诊断中的应用

目的 评价普通CT 2 mm薄层骨算法扫描在肺部病变诊断及鉴别诊断中的价值。方法 本组135例患者存常规CT 10 mm标准重建扣描后对感兴趣区行2 mm薄层骨算法扫描。结果 本组135例患者通过10 mm标准重建与2 mm薄层骨算法重建扫描的CT征象对照(表1—3)认为2 mm薄层骨算法扫描可清晰显示肺部中轴间质及小叶间隔、胸膜下线影、蜂窝症、2～5mm小结节孤立结节内成分、边缘征象及支气管壁改变．结论 通过对135例患者肺部病变的影像分析、研究认为普通CT 2 mm薄层骨算法扫描较10 mm标准重建扫描能更好、更清晰的显示肺部病变的各种征象，在诊断肺部孤立或弥漫性结节病灶、肺部弥漫性间质病变及气道性病变的诊断中具有与高档螺旋CT高分辨扫描相似的价值，对基层医院CT检查工作的开展，具有实用性和推广性。