微血管扩张法评价丹参不同提取工艺

目的:优选丹参的提取工艺。方法:应用Powerlab血管张力测定系统,采用药效学指标考察丹参的不同提取工艺对血管张力的影响,比较提取工艺的优劣。结果:优选出丹参水超声醇沉提取工艺,实验结果可靠。结论:水超声醇沉提取法能明显降低氯化钾(0.006mol.L-1)和重酒石酸去甲肾上腺素注射液(NE)(1×10-5mol.L-1)引起的收缩,舒张率分别达到(9.7±12.4)%,(7.9±10.7)%,和模型组比较差异有显著性(P<0.05)。     

柴胡剂对单克隆抗体5—1—6引起蛋白尿的实验研究

静脉一次性注射单克隆抗体5-1-6可引起实验大鼠大量的、一过性的蛋白尿。抗体注射后第1、3、5、8天,柴胡剂治疗组与PBS对照组比较有显著性差异。免疫荧光所见,柴胡剂治疗组中沿肾小球毛细血管壁颗粒样沉积明显减少。口腔灌药柴苓汤对蛋白尿的作用未能得到证实。此实验提示柴胡剂可能通过某种途径改变了大鼠肾小球毛细血管壁的通透性。     

Cardiovascular actions of Radix Stephaniae Tetrandrae: a comparison with its main component, tetrandrine

A comparison of the cardiovascular actions of the extract of Radix Stephaniae Tetrandrae (RST), the root of a Chinese herb Stephania tetrandra S Moore, in rats with those of tetrandrine (Tet), the best known active component of RST was reviewed. The RST extract inhibits Ca2 influx into the myocyte and reduces protein release during reperfusion with a Ca2 containing solution following perfusion with a Ca2 free solution (Ca2 paradox) , and arrhythmia during reperfusion in the isolated perfused heart. It also reduces the infarct size induced by ischemia/reperfusion in vitro and in vivo. In addition, the RST extract suppresses elevation of arterial blood pressure in DOCA-salt hypertensive rats. It does not further reduce the heart rate and coronary flow significantly during myocardial ischemia. The effects are similar to those of Tet. When compared with the same doses of Tet alone, the RST extract, of which 9 % is Tet, produces equally potent effects on infarction, arrhythmias, coronary flow and heart r     

地榆炒炭的组织结构及化学变化

通过组织结构及薄层层析等实验研究,证明地榆炒炭前后药材内部变化。地榆炭与原药材比较,草酸钙结晶明显减少,部分组织炭化,有效成分地榆皂甙及鞣质受到一定程度破坏,但又产生了一些新的成分。     

含大黄的中成药中蒽醌类化合物的多组分定性定量研究

本文采用反相－ＨＰＬＣ方法对含大黄的中成药之四种不同剂型（丸、片、汤和洗剂）共九个样品进行大黄蒽醌类化合物的多组分定性定量分析。结果表明，在本文所用的样品制备方法和色谱实验条件下，２０ｍｉｎ左右即能完成一次色谱分析，大黄中的五个蒽醌甙元均能获得满意的定性定量，方法简便易行，相对标准误和加样回收率均较好。     

板蓝根甲素的结构分析

板蓝根甲素的结构分析王树春（陕西医学高等专科学校，西安７１００６８）吕扬１）郑启泰吴寿金２）桂承会２）孔漫梁晓天（中国医学科学院中国协和医科大学药物研究所，北京１０００５０）关键词板蓝根甲素；Ｘ衍射；综合分析以新鲜的菘蓝根为原料，洗净、粉碎，用９５％...     

Suppressive effects of<Emphasis Type="Italic">Platycodon grandiflorum</Emphasis> on the progress of carbon tetrachloride-lnduced hepatic fibrosis

The suppressive effects of Platycodi Radix (Changkil: CK), the root of Platycodon grandiflorum A. DC (Campanulaceae), on the progress of acute carbon tetrachloride (CCl4)-induced hepatic fibrosis were investigated in the rat. CK significantly suppressed CCl4-induced hepatic necrosis and inflammation, as determined by the serum enzymatic activities of alanine and aspartate aminotransferase and serum tumor necrosis factor-alpha levels, in dose-dependent manners. In addition, the increased hepatic fibrosis after acute CCl4 treatment was suppressed by the administration of CK. CK also significantly prevented the elevation of hepatic alpha1 (I) procollagen (type I collagen) mRNA and alpha-smooth muscle actin (alpha-SMA) expressions in the liver of CCl4-intoxicated rats and also suppressed the induction of alpha-SMA and type I collagen in cultured hepatic stellate cells, in dose-dependent manners. These results suggest that the suppressive effects of CK against the progress of acute CCl4-induced hepatic fibrosis possibly involve mechanisms related to its ability to block both hepatic inflammation and the activation of hepatic stellate cells.     

十七种不同产地白头翁的质量考察

目的以白头翁皂苷B4对照品作为指标,考察不同产地白头翁的质量真伪优劣。方法TLC、UV、HPLC方法。结果白头翁皂苷B4含量≥5.0%有九种,≤5.0%有8种,其中伪品4种。结论安徽滁州产地道药材白头翁含量高达8.0%,从而证实了地道药材的名副其实。     

HPLC法测定红穿破石中大黄素的含量

目的建立红穿破石中大黄素含量的高效液相色谱测定方法。方法选用EclipseXDB-C18色谱柱(4.6mm×150mm,5μm),以甲醇-0.1%磷酸溶液(75∶25)为流动相,检测波长为285nm。结果大黄素在0.0063～0.063μg范围内呈良好的线性关系(r=0.9999),平均回收率为102.0%,RSD=1.58%(n=6)。结论本法简便,准确,可用于红穿破石中大黄素的含量测定。     

黄芪对低氧性肺动脉高压大鼠肺组织血栓素A_2及肺血管结构的影响

目的:探讨黄芪对低氧性肺动脉高压(HPH)大鼠肺腺泡内肺动脉结构重建及肺组织血栓素(TXA2)的影响。方法:雄性Wistar大鼠21只,随机分为3组,每组7只。对照组:室内空气正常饲养;低氧组:在常压低氧舱内(10±0.5)%饲养;治疗组:缺氧+黄芪(12g/kg)。低氧30天分别用右心导管法测定肺动脉平均压(mPAP),放射免疫法测定肺组织中TXA2含量,计算MA、PMA、NMA比例及肺中、小肌型动脉相对中膜厚度(RMT)与相对中膜面积(RMA)。结果:低氧组mPAP、RVHI、TXA2含量较对照组明显升高(P<0.01或P<0.05),治疗组较低氧组明显减少(P<0.05)。低氧组肺小动脉肌化程度、RMT(%)、RMA(%)较对照组明显增多(P<0.01),治疗组较低氧组明显减少(P<0.01)。结论:慢性低氧可引起大鼠肺血管结构重建,黄芪注射液可降低肺组织TXA2含量,可部分逆转肺血管结构重建,降低肺动脉高压。