益肾乌发口服液对小鼠急性肝毒性"量-时-毒"关系研究

目的 研究益肾乌发口服液单次给药致小鼠急性肝毒性的"量-时-毒"关系.方法 "时-毒"关系研究:小鼠灌胃一定剂量的益肾乌发口服液,分别于给药后不同时间检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBI)水平及肝、脾、胸腺等脏器指数的变化."量-毒"关系研究:给小鼠灌胃不同剂量的益肾乌发口服液,于给药后2h按"时-毒"研究方法对小鼠进行相应处理.结果 小鼠血清ALT、AST、TBI水平均在灌胃较高剂量的益肾乌发口服液后2h达到高峰,给药后12h肝脏指数升至最高,给药后72h可恢复至正常值;剂量在(4.46～25)mL·kg-1之间肝脏可产生明显病理损伤,且随着剂量增大,ALT、AST、TBI水平升高显著.结论 小鼠单次灌胃一定剂量的益肾乌发口服液可造成急性肝毒性损伤,并呈现明显的"量-时-毒"关系.     

益柏外洗方的动物实验研究

目的：探讨益柏外洗方对动物皮肤的过敏性、刺激性及急性毒性。方法：大鼠30只,雌雄各半并随机分组;致敏接触受试药并观察大鼠机体免疫系统对益柏外洗方的反应,计算致敏率、评分,评估大鼠对益柏外洗方的过敏性。小鼠25只,雌雄兼用;两者均随机分组,刺激性实验用10只,急性毒性实验用15只。小鼠背部敷益柏外洗方后观察小鼠局部表现、全身中毒以及死亡情况,判断益柏外洗方对小鼠皮肤的刺激性和急性毒性。结果：大鼠敷药皮肤未见水肿红斑,其各项生理指标等均未见明显异常,表明益柏外洗方对大鼠免疫系统影响微小,无过敏性。两组用药小鼠敷药区皮肤及全身未见红斑和水肿等病变,证实益柏外洗方对小鼠皮肤急性毒性很小,无刺激性。结论：益柏外洗方对实验动物皮肤的急性毒性很小、无过敏性及刺激性,具有临床实用价值。     

《医验大成》脾胃病证治特色

《医验大成》是明代著名医家秦昌遇的著作之一,该书对秦氏一些精华医案进行整理,有极高的临床参考价值,特别是重视脾胃病的辨证治疗,临证有自己的独到体会。文章将就《医验大成》的脾胃病证治特色进行探讨。     

陈意运用加减不换金正气汤治疗湿阻经验

陈师根据多年临床经验,在不换金正气散基础上加减创立加减不换金正气汤,用于治疗慢性胃炎、胃溃疡、结肠炎、失眠等湿热阻滞中焦之证,并认为湿热之证,治疗当苦寒参以苦温,方能化其湿,泻其热,同时强调应慎用健脾益气之品,以防助湿生热。     

脂溢洗方外洗加当归苦参丸内服治疗头部脂溢性皮炎的疗效观察

目的:观察脂溢洗方外洗加当归苦参丸内服治疗头皮脂溢性皮炎的临床疗效。方法:将头部脂溢性皮炎患者102例随机分为两组。治疗组51例,用脂溢洗方外洗,中成药当归苦参丸内服;对照组51例,用采乐洗剂外洗,抗组胺药、B族维生素口服。结果:治疗组总有效率为86.3%,对照组为68.6%。两组比较,差异有显著性意义(P<0.05)。结论:脂溢洗方外洗加当归苦参丸口服治疗头部脂溢性皮炎的临床疗效较好。     

从《四部医典》看汉、藏医德学之渊源

《四部医典》是藏医学发展史上最伟大的成果之一,它辩证地吸收了汉、藏医学著作的精华,总结了藏医药的临床经验,与中医学有着密切的源流关系。其对医德学的论述与中医医德学思想之间也有着紧密的渊源关系,唐蕃和亲及文化交流和吐蕃统治者对医学发展的重视是二者渊源关系存在的主导性因素。     

四川凉山20-30岁彝族人群指长与身高的相关性研究

目的研究四川凉山彝族人群指长与身高的相关关系。方法按照人体骨骼测量方法,测量208例(男151,女57)20-30岁四川彝族健康成年人左手五指长度、五指末端指节长度及身高。结果与结论男性左手10个测量指标与身高均有显著相关性,其中中指长与身高相关性最大(r=0.372)。女性只有食指长、中指长、环指长、小指长及食指末节长与身高有显著相关性,以食指末节长与身高的相关性最大(r=0.454)。     

Allele frequencies of mitochondrial D-loop (CA)n repeat polymorphism in six Chinese ethnic groups

Allele frequencies of mitochondrial D-loop (CA)(n) repeat was carried out in six Chinese ethnic groups: Han ethnic group in Beijing, Uygur ethnic group in Kashi of Xinjiang province, Li ethnic group in Hainan province, Yao ethnic group in Junan of Guangxi province, Tibet ethnic group in Lasa of Xizang province and Yi ethnic group in Honghe of Yunnan province. A total of 542 individuals were typed. Comparative analyses between our population data and other populations gathered from the literature were performed.     

Hepatoprotective effects of Yi Guan Jian, an herbal medicine, in rats with dimethylnitrosamine-induced liver fibrosis

Aims of the study

Yi Guan Jian (YGJ) has long been employed clinically to treat liver fibrosis in traditional Chinese Medicine but the mechanism underlying the regulation has not been clarified in detail. The present investigation was designed to assess the involvement of the fibrosis pathway in dimethylnitrosamine (DMN)-induced liver fibrosis in rats.

Materials and methods

Liver fibrosis was induced by DMN injection (10 mg/kg, i.p., given three consecutive days each week) following 4 weeks. YGJ was oral administered (1.8 g/kg daily via gastrogavage for two weeks). Liver sample were subjected to histological and western blot studies. For evaluation of hepatic fibrosis-related factors, collagen α1-I, tissue inhibitor of metalloproteinase-1 (TIMP-1), and α-smooth muscle actin (α-SMA) mRNA and protein levels were analyzed.

Results

YGJ remarkably prevented body weight loss and DMN damage in the liver, and it inhibited the elevation of serum glutamate oxaloacetate transaminase (GOT), and glutamic pyruvic transaminase (GPT). Oral administration of YGJ extract significantly reduced the accumulation of collagen α1-I, TIMP-1, and α-SMA in liver tissues.

Conclusions

Taken together, these findings indicate that the YGJ Chinese herb showed hepatoprotective and anti-fibrogenic effects against DMN-induced hepatic injury. Our data suggest that the YGJ may be useful in reversing the development of hepatic fibrosis.     

补肾益脑汤治疗脑梗死的实验研究

目的探讨中药补肾益脑汤对实验性脑梗死大鼠脑细胞保护的作用机理。方法用线栓法制作大鼠大脑中动脉阻断（MCAO）模型,具有脑梗死临床特征者为脑梗死大鼠模型,共50只。对模型随机分为模型组、对照组、治疗高剂量组、治疗低剂量组各10只,同时设正常对照组10只,用药30天后取出大脑,观察梗塞灶周围脑组织中bFGF及VEGF表达情况。结果通过治疗实验性脑梗死大鼠的观察,该药对脑细胞保护已能达到治疗目的。结论补肾益脑汤对实验性脑梗死大鼠有治疗作用。