复方罗布麻片联合天麻头痛片治疗原发性高血压病108例的临床观察

目的观察复方罗布麻片联合天麻头痛片治疗原发性高血压病的临床疗效。方法将200例原发性高血压病患者随机分为两组,其中观察组108例,给予口服复方罗布麻片联合天麻头痛片;对照组92例按西医门诊个体化方法选用常规西药治疗,以4周为1个疗程,观察2个疗程。结果观察组显效68例,有效30,无效10例,不良反应1例,总有效率为90.70%;对照组显效52例,有效20,无效20例,其中不良反应8例,总有效率为73.90%,两组疗效P<0.05,差异有统计学意义。结论复方罗布麻片联合天麻头痛片治疗原发性高血压病的临床疗效理想,可以在临床上推广应用。     

人参皂苷对制动所致大鼠骨骼肌萎缩的保护作用

目的探讨人参皂苷对制动所导致的萎缩骨骼肌结构及功能的影响。方法选用SD大鼠,随机分为3组,对照组(CON)、制动组(IMM)和制动加人参皂苷处理组(GIN),每组10只。制动组采用石膏固定后肢模型,人参皂苷处理组在制动基础上按50 mg/kg·d体重腹腔注射人参皂苷,同步对照组及后肢制动组每日腹腔注射同等体积的生理盐水,共3周。实验结束观察大鼠比目鱼肌重量变化;通过Western blot观察骨架蛋白dystrophin和desmin的表达变化;通过离体肌条实验观察骨骼肌收缩功能的变化。结果制动组比目鱼肌湿重显著低于对照组及人参皂苷处理组;制动组比目鱼肌dystrophin表达显著降低,desmin降解明显增强,人参皂苷处理后上述改变明显缓解;制动组比目鱼肌收缩功能显著低于对照组及人参皂苷处理组。结论人参皂苷可有效防护制动所导致的骨骼肌萎缩。     

不同方案治疗肺结核的疗效比较

目的 探讨不同方案治疗肺结核的临床疗效.方法 随机选取我院2011年5月-2012年5月收治的22例肺结核患者为观察组,选取2010年5月-2011年5月收治的肺结核患者22例为对照组,对照组采用标准短程化疗方案2HRZS/4HR,治疗组患者采用吡嗪酰胺联合标准化疗方案,并观察两组患者治疗结果.结果 治疗6个月后,治疗组22例患者中阴转率为90.90%,病灶吸收率为86.36%,均高于对照组的痰菌阴转率72.73%和病灶吸收率63.64%,差异有统计学意义（P＜0.05）.治疗组4例患者发生不良反应,不良反应发生率为18.18%,对照组有5例发生不良反应,不良反应发生率为22.72%;两组患者的不良反应发生率比较,差异无统计学意义（P＞0.05）.结论 临床对肺结核患者实施吡嗪酰胺联合标准化疗方案治疗有效率高,不良反应少,效果显著,值得临床推广使用.     

人性化护理在普外科护理中的应用

目的：分析人性化护理在普外科护理中的应用效果。方法将2010年3月~2014年5月期间本院收治的124例普外科手术患者,随机将其均分为观察组62例及对照组62例。其中观察组患者给予人性化护理,对照组患者给予常规护理,对比分析两组患者的护理满意度、并发症发生率以及住院时间。结果在经过不同护理模式后,观察组患者护理满意度为100.0%（62/62）,并发症发生率为4.8%（3/62）,住院时间为（7.5±1.6）d;对照组患者护理满意度为75.8%（47/62）,并发症发生率为14.5%（9/62）,住院时间为（14.3±2.1）d。两组间比较差异均有统计学意义（P<0.05）。结论在普外科手术中,对患者实行人性化护理能够进一步提升患者的护理满意度,提升患者对相关疾病知识的了解,从而降低患者的住院时间,在临床中值得推广使用。     

Older Chinese migrants in coronavirus pandemic: exploring risk and protective factors to increased loneliness

Older migrants may be one of the most vulnerable populations during the coronavirus pandemic, yet the degree of impact remains largely unknown. This study explores (1) the consequences of the coronavirus pandemic for older Chinese migrants in Belgium and the Netherlands in terms of increased loneliness and its risk factors (reduced in-person contact, decreased social participation, feelings of existential threat) and protective factors (increased non-in-person contact, more individual activities), and (2) which risk and protective factors have contributed to the incidence and prevention of higher loneliness levels. Using quantitative data of a survey among 98 Chinese migrants aged 50 years and older in Belgium (n = 84) and the Netherlands (n = 14), the findings first indicate that the coronavirus pandemic has a significant impact on older Chinese migrants’ lives. One in five experienced more loneliness. Second, reduced social participation (measured as less frequent participation in outdoor group activities) and financial insecurity (measured as experiencing financial difficulties) lead to higher than pre-pandemic loneliness levels. Problem-focused coping strategies (measured as increased non-in-person contact, via telephone or social media) and emotion-focused coping (measured as finding distraction through increased participation in individual activities) were not found to protect against increased loneliness in the pandemic. Two practical implications for loneliness interventions for older Chinese migrants are put forward. Organizing COVID-19-safe social participation activities and paying more attention to older Chinese migrants’ financial situation can be beneficial when addressing higher levels of loneliness due to the coronavirus pandemic.     

日本血吸虫23 kDa膜蛋白DNA疫苗和蛋白质疫苗联合应用免疫保护性作用的研究

目的　研究 Sj C2 3DNA疫苗和 Sj C2 3蛋白疫苗联合免疫对 C5 7BL/ 6感染小鼠的免疫保护作用。方法　分别构建和制备 Sj C2 3DNA疫苗 (pc DNA3.1- Sj C2 3)和蛋白疫苗 (Sj C2 3大亲水片段融合蛋白 GST- HD)。 4 8只 C5 7BL / 6小鼠随机分为 A、B、C、D 4组 ,每组 12只。 A组 (Sj C2 3DNA+GST- HD联合应用组 )每只小鼠分别在第 0、2周经股四头肌注射 10 0μg Sj C2 3质粒 DNA,在第 4周经背部皮下多点注射 5 0 μg GST- HD+5 0 μg CFA;B组 (Sj C2 3DNA组 )每只小鼠分别在第 0、2、4周经股四头肌注射 10 0 μg Sj C2 3质粒 DNA;C组 (蛋白疫苗组 )于第 4周每鼠经背部皮下多点注射5 0μg GST- HD+5 0μg CFA 1次 ;D组 (对照组 )每鼠分别在第 0、2、4周肌注 10 0μg pc DNA 3.1。各组在末次免疫后 30 d每鼠以 4 5± 2条 /只日本血吸虫尾蚴攻击感染 ,攻击后 4 5 d剖杀小鼠 ,计数成虫及肝内虫卵数。在免疫前、末次免疫后和攻击后从小鼠尾静脉采血 ,分离血清 ,用 Western- blot法检测抗体反应。结果　联合组与质粒对照组相比 ,获得了 36 .9%的减虫率和 30 .7%减卵率 ;而单独Sj C2 3DNA疫苗组的减虫率为 2 6 .9% ,显著低于联合组 (P<0 .0 5 )和 2 2 .2 %的减卵率 ,单独蛋白疫苗组 (GST- HD)为 15 .2 %和 12 .2 %     

中药复方金三莪治疗肝纤维化的临床研究

目的:观察中药复方金三莪在慢性乙型肝炎患者中抗肝纤维化的疗效.方法:选取147例慢性乙型肝炎患者,随机分为中药组、γ-IFN组及对照组,分别采用金三莪、γ-IFN及一般护肝治疗.观察治疗前后及随访3个月时,各组患者症状、体征、肝功能、肝纤维化指标及B超影像学变化,按非创伤性指标的肝纤维化疗效评估标准,评价中药复方金三莪抗肝纤维化疗效.结果:中药组患者治疗后总有效率为80.8%,与对照组(41.3%)比较差异有显著性意义(P＜0.01),而与γ-IFN组(81.6%)比较差异无显著性意义(P＞0.05);中药组患者治疗后肝功能指标、肝纤维化指标、B超影像学结果均较对照组有明显的改善(P＜0.01);与γ-IFN组患者比较差异无显著性意义(P＞0.05),随访3个月各项指标仍维持稳定,治疗过程中未出现不良反应.结论:中药复方金三莪能有效降低患者肝纤维化指标,改善患者肝功能,有明显的抗肝纤维化疗效,与γ-IFN组相比抗肝纤维化疗效差异无显著性意义,且无γ-IFN所易致的发热、骨髓抑制等副作用,值得在临床上广泛应用.     

Induction of 8-azaguanine-resistant mutation and neoplastic transformation of hamster embryonic cells by coadministration of sodium nitrite and aminopyrine

Summary Hamster embryos in utero on the 11th or 12th day of gestation were treated simultaneously with aminopyrine (Ap) and sodium nitrite (NaNO₂) by oral administration of the compounds to the mothers by stomach tube. For measurement of induction of 8 AG-resistant mutations, the embryonic cells from treated and control mothers were cultured in MEM plus 10% FBS for 72 h and then selected in medium containing 10 or 20 g/ml of 8 AG. The number of 8 AG-resistant colonies was markedly increased after co-administration of Ap and NaNO₂, and slight induction of mutations was also observed in cells from mothers given NaNO₂ alone. This treatment also caused morphological or malignant transformation of cultured cells. About 5-to 6-fold increase in the number of transformed colonies was observed in cells from mothers given Ap plus NaNO₂. Cells from the transformed colonies produced tumors when implanted into the cheek pouches of young golden hamsters. These tumors were diagnosed as pleomorphic fibrosarcomas. Similar results were obtained with cells from embryos treated transplacentally with NDMA as positive controls. A single transplacental oral application of Ap at 200 mg/kg or of NaNO₂ had only slight biological actions to the cultured embryonic cells. NDMA was produced in the stomach of animals treated simultaneously with Ap and NaNO₂. A small amount of NDMA was also detected in the stomach after a single dose of NaNO₂.Abbreviations 8 AG 8-azaguanine - NaNO₂ sodium nitrite - Ap aminopyrine - NDMA N-nitrosodimethylamine - MEM Eagle's minimal essential medium - FBS fetal bovine serum - DMEM Dulbecco's modified Eagle's medium - DCM dichloromethane This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science, and Culture, Japan, and a Grant from the Ministry of Health and Welfare, Japan     

日本血吸虫pcDNA3.1(+)/MLP核酸疫苗对家兔的保护性免疫作用

目的 观察日本血吸虫pcDNA3.1( )／MLP(SJP)核酸疫苗对家兔的保护性免疫作用。方法 将24只新西兰家兔随机分为2组，每组12只；实验组每只家兔SJP的免疫剂量为500μg／次，后腿肌肉注射，对照组注射空质粒pcDNA3.1( )；隔周免疫1次，共4次，最后1次免疫后2周进行日本血吸虫尾蚴攻击感染。尾蚴攻击感染60d后处死，观察肝脏病变，计算减虫率及减卵率，并做血清抗体及细胞因子分析。结果 SJP免疫家免可明显抑制肝脏虫卵肉芽肿的大小，肉芽肿数量减少，肝组织减卵率为28．10％，并可诱导IgA、IgG1变化，诱生INF-γ应答。结论 SJP免疫家兔对日本血吸虫尾蚴攻击感染可产生一定的保护作用。     

日本血吸虫(大陆株)14-3-3 epsilon同型信号转导蛋白诱导小鼠保护性免疫的研究

目的：探索日本血吸虫新的疫苗候选分子对小鼠的保护性免疫效果。方法：利用已构建的真核表达质粒pBK-Sj14-3-3在大肠杆菌ＢＬ２１内表达的日本血吸虫（大陆株）14－3－3epsilon同型融合蛋白质，免疫6周龄BALB／c雌性小鼠，免疫3次，每次间隔2周，第3次免疫后2周感染尾蚴。42d后剖杀小鼠，计算其减虫率和肝减卵率。结果：各免疫组与对照组相比，均获得了部分抗血吸虫保护性免疫力，第1、2、3各免疫组的减虫率分别为32．7％（P＜0．05）、30．7％（P＜0．05）、27．5％（P＜0．05）；其肝减卵率分别是48．5％（P＜0．05）、43．1％（P＜0．05）、28．2％（P＜0．05）。结论：首次证明日本血吸虫14－3－3epsilon同型重组融合蛋白可诱导小鼠抗血吸虫感染的部分保护性免疫力。