青皮对大鼠离体结肠平滑肌运动的影响

观察青皮对离体结肠平滑肌运动的影响。方法：用生理记录仪同时记录安置在各灌流肌槽中的大鼠结肠头端和尾端纵，环行肌肌条的收缩活动。结论：青皮可能通过对平滑肌的直接作用或其他途径来抑制大鼠离丛结肠平滑肌条的收缩活动。     

化学模式识别评价中药威灵仙质量的研究

本文按威灵仙所含化学成分对6个种、16个产地的21个样品进行了气相色谱分析;以抗炎药理活性作为评价质量的指标,将样品分为有抗炎作用和无抗炎作用两大类,一部分作训练集,一部分作试验集;用SIMCA选择特征并分类,再用PCA将选择的特征数据在二维平面上显示;根据分类和显示的结果,可以区分威灵仙样品有无抗炎作用即质量的优劣。     

Usefulness of 31P-MRS as a method of evaluating the viability of preserved and transplanted rat liver

We used phosphorus-31 magnetic resonance spectroscopy (³¹P-MRS) to evaluate the viability of transplanted rat liver. Wistar rats were used as donors and recipients. The donor livers were preserved in saline (group 1), Euro-Collins solution (group 2), or in University of Wisconsin (UW) solution (group 3) for 3 and 6 h in groups 1, 2 and 3 and for 9 h in groups 2 and 3. Thereafter the livers were orthotopically transplanted. ³¹P-MRS spectra were measured after portal reperfusion. Finally, all the recipients were divided into survivors and non-survivors. Survival rates were better in group 3 than in groups 1 and 2. In the 9-h-preserved livers, the livers in group 3 showed a significantly higher β-ATP/Pi ratio than those in group 2. Comparing survivors and non-survivors in the 6-h-preserved livers in group 2, survivors' livers showed significantly higher (3-ATP/Pi ratio than those of non-survivors. We concluded that ³¹P-MRS is a useful method for assessing viability of rat liver grafts.     

痿康口服液雄性激素样作用的研究

①目的为痿康口服液治疗阳痿提供动物实验资料，加速该方剂开发利用。②方法通过采用雏鸡冠法、大鼠包皮腺法及痿康口服液对正常大白鼠性器官重量的影响，测定痿康口服液的雄性激素样作用。③结果痿康口服液各剂量组均使动物附性器官湿重明显增加（ｔ＝４．２０～１１．１３，Ｐ＜０．０１）。④结论痿康口服液有显著的雄性激素样作用。     

Myocardial infarction before age 36 years in women: Predominance of apparent nonatherosclerotic events

To examine whether myocardial infarction (MI) in young women resulted from atherosclerosis, we studied 19 women aged 18 to 35 years who had documented MI at our institution from 1955 to 1979. They were compared to 20 similar men who were selected randomly. All 20 men had at least one major coronary risk factor; six of the women did not (p < 0.01). The risk factors were up to four times more common in men. Coronary anatomy, defined in 13 women and 13 men, demonstrated patent vessels or single stenotic disease in 10 (77%) women but in only one (8%) man, and multilesion disease in only three (23%) women but in 12 (92%) men (p < 0.005). All with patent coronaries were women. Thus atherosclerosis is more advanced and accounts for most myocardial infarcts in young men, but is less evident and accounts for only a minority of MI in young adult women, who have a greater preponderance of nonatherosclerotic related infarcts.     

健脾扶运汤的“健脾”作用研究

本研究表明，健脾扶运汤对家兔离体肠管运动有双向调节作用，当肠管强直性收缩时有解痉作用，当肠管活动处于抑制状态时则有兴奋作用。对小鼠小肠推进运动有促进作用。对脾虚模型小鼠能明显改善症状和增加体重。因此，调整胃肠运动功能可能是健脾扶运汤“健脾’作用的主要机理。     

脾虚型慢性萎缩性胃炎疗效影响因素的COX模型分析

目的：分析中药治疗脾虚型慢性萎缩性胃炎疗效的影响因素。方法：随访236例脾虚型慢性萎缩性胃炎患者应用COX危险比例模型分析疗效的影响因素。结果：单因素分层分析结果：年龄（X2）,职业（X3）,胃窦病理程度（X11）,疗程（X6）,不同辨证分型对本病疗效影响显著,将20个因素全部进入COX模型,应用逐步上升法筛选各显著因素,得实际较优模型,h(t,x)=h0(t)exp(0.5502x11-0.3247x1-0.0149x1-0.0149x2-0.3101x20),由模型可知性别（X1）,年龄（X2）,胃窦病理程度（X11）, 脾虚血瘀症（X20）对本病疗效影响较大。结论：通过以上分析,认为重视CAG的病理生理特点和合理应用活血化瘀疗法是提高本病疗效的重要途径之一。     

"阴火"辨惑

对李东垣《脾胃论》所提出的“阴火”之论,以及傅衍魁等主编《医方发挥》对“阴火”的解释,并结合明代诸多医家对“阴火”的理解,加以分析辨惑,强调元气盛衰与“阴火”关系最为密切。     

耳科玄府说及加味通气散开通玄府的微循环机制 --张志远学术经验系列(一)

目的探讨张志远教授治疗耳鸣、耳聋验效方加味通气散(JWTQS)开通听户玄府的微循环机制.方法解剖显微镜下观察记录JWTQS高、低两个剂量对大鼠脑膜微循环及肠系膜微循环的影响.结果JWTQS两剂量均可显著改善肾上腺素(AD)滴注后脑膜微循环的血色、血液流态和血液流速,与生理盐水组比较差异有显著性(P＜0.01);JWTQS两剂量对AD滴注前后对毛细血管交点数和管径无显著影响(P＞0.05),但可显著降低AD滴注前后毛细血网交点数和管径的差值,与生理盐水组比较差异有显著性(P＜0.01).结论加味通气散对微循环的调节作用可能是其治疗耳鸣、耳聋的主要治疗学机制之一,表明开通听户玄府法有其科学内涵.     

RP-HPLC法测定丰城鸡血藤提取物中大豆黄酮、染料木素、刺芒柄花素和美皂异黄酮

目的研究RP-HPLC法分离丰城鸡血藤提取物的条件并建立其中4种异黄酮活性成分的测定方法。方法以乙腈-0.1%磷酸为流动相,采用Spherisord ODS柱(250mm×4mm,5μm),梯度洗脱,体积流量0.8mL/min,检测波长260nm,研究流动相组成和梯度变化对丰城鸡血藤提取物分离效果的影响。结果大豆黄素、染料木素、刺芒柄花素和美皂异黄酮的线性范围、相关系数和平均加样回收率分别为6.24~31.2μg/mL、r=0.9999、99.32%;10.00~130.0μg/mL、r=0.9997、102.20%;5.99~125.9μg/mL、r=0.9999、100.08%和6.96~34.8μg/mL、r=0.9993、99.52%。各组分的加样回收率RSD分别为1.79%、1.51%、1.54%和2.01%(n=5)。结论该法可用于丰城鸡血藤提取物中成分的分离及其中4种异黄酮活性成分的测定。