Preventive Effect of Matrix Metalloproteinase Inhibitor, R-94138, in Combination with Mitomycin C or Cisplatin on Peritoneal Dissemination of Human Gastric Cancer Cell Line TMK-1 in Nude Mice

R-94138, a matrix metalloproteinase inhibitor, was examined for the ability to prevent peritoneal dissemination of a human gastric cancer xenograft, TMK-1. When the supernatant of a co-culture of TMK-1 cells and human normal fibroblast cells was subjected to gelatin zymography, it was clear that the protein expression of MMP-2 had been inhibited by R-94138. When TMK-1 was injected intraperitoneally (i.p.) into nude mice at 5×10⁵ cells/body, the resulting peritoneal dissemination mimicked clinical carcinomatous peritonitis. When the maximum tolerated dose of mitomycin C (MMC) or cisplatin (DDP) was given 12 h after the tumor inoculation, peritoneal dissemination was completely inhibited, while the effect of R-94138 was limited when it was given i.p. at a dose of 20 mg/kg in a schedule of q.d. ×5 starting 12 h after tumor injection. MMC and DDP also suppressed peritoneal dissemination when they were administered 1 week after the tumor inoculation at a single dose of 2 and 3 mg/kg i.p., respectively. R-94138 inhibited peritoneal dissemination when it was administered i.p. at a dose of 30 mg/kg in a schedule of q.d. ×5 starting from 1 week after tumor injection. The combination of MMC and R-94138 increased the preventive effect on peritoneal dissemination. R-94138 seems to be a promising candidate to prevent peritoneal dissemination of gastric cancer.     

人胃癌细胞腹膜粘附影响因素的实验研究

目的 探讨影响人胃癌细胞在腹膜粘附的因素,为临床上采取有效措施,减少腹膜转移的发生提供依据。方法 用人胃癌细胞株在小鼠腹膜上培养,以ＭＴＴ法检测粘附细胞数的体外实验模型,观察腹膜损伤,血液成分和癌胚抗原（ＣＥＡ）对胃癌细胞在腹膜粘附的影响。结果 腹膜损伤组的粘附细胞数（ＡＤ）较对照组明显增高（Ｐ〈０．０１）,血液中的白细胞,血小板组ＡＤ值较对照组明显增高（Ｐ〈０．０１）,血浆组ＡＤ值亦较对照组增高     

Is there an oncological interest in the combination of CRS/HIPEC for peritoneal carcinomatosis of HCC? Results of a multicenter international study

Introduction

Peritoneal metastasis (PM) of hepatocellular carcinoma (HCC) without distant spread are rare. The related prognosis is poor without standard treatment available. The role of cytoreduction surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) is poorly documented.

Cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy (HIPEC) in patients with gastric cancer and peritoneal carcinomatosis

Background

Gastric Cancer (GC) with Peritoneal Carcinomatosis (PC) has long been regarded as a terminal disease. Over the past two decades, cytoreductive surgery (CRS) with hyperthermic intraperitoneal chemotherapy (HIPEC) has changed the traditional concept of peritoneal metastases from being a systemic disease, to being considered a locoregional dissemination.

Postoperative abdominal infections after resection of T4 colon cancer increase the risk of intra-abdominal recurrence

Introduction

Patients with pT4 colon cancer are at risk of developing intra-abdominal recurrence. Infectious complications have shown to negatively influence disease free survival (DFS) and overall survival (OS) in stage I-III colon cancer. The aim of this study was to determine whether surgical site infections (SSIs) also increase the risk of intra-abdominal recurrence in pT4 colon cancer patients.

序贯透析治疗肾功能衰竭合并急性左心力衰竭的临床应用

目的观察序贯透析对肾功能衰竭合并心力衰竭、肺水肿患者的治疗效果。方法对13例肾功能衰竭合并急性心力衰竭、肺水肿的患者行序贯透析疗法,观察其临床症状、体征,分析其疗效。结果痊愈7例,显效5例,有效1例,总有效率为100.0%。结论序贯透析能迅速缓解肾功能衰竭合并急性左心力衰竭患者的临床症状,改善患者的心功能状况,是抢救肾功能衰竭合并急性左心力衰竭的有效方法 。     

Characterization of biological activity of Scatophagus argus venom

Scatophagus argus of the family Scatophagidae inflicts painful wounds in fishermen while handling it. The venom induces prominent local tissue damage characterized by pain, edema and necrosis. The pathogenesis of acute muscle damage in gastrocnemius muscle induced by S. argus venom was studied in mice. The inflammatory response induced by S. argus venom in the mice hind paw was studied measuring paw edema. Intramuscular injection of S. argus venom induced motoxicity. The effect of S. argus venom on the cellular components of inflammatory response was investigated. Venom from S. argus were quantitatively analyzed for enzymic and biochemical activity. The biochemical changes induced by the sublethal concentration of S. argus venom and histopathological studies of effect of venom on mice were carried out. Venom induced a rapid increment in serum creatine kinase (CK) and lactate dehydrogenase (LDH) showing the myotoxicity of venom. Concomitant with this a reduction of muscle CK and LDH activity was observed, where as no increment in muscle lactate was detected. Our findings showed that the edematic activity was dose dependent and remained significantly elevated over 48 h after injection. Administration of S. argus venom caused a significant cell accumulation of neutrophils in to peritoneal cavity as well as foot pad up to 24 h with maximal being at 4-6 h. The venom components analyzed showed the presence of phosphodiesterase, acid phosphatases, alkaline phosphatases, proteinase, and caseinolytic activity. SDS PAGE revealed the presence of major and minor protein bands between 6.5 and 68 kDa. The biochemical changes induced by the sublethal concentration of S. argus venom showed reversible changes in the hematological (blood cell count, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin and platelet count) parameters which were significantly altered at 6 and 24 h (GLM repeated measures p < 0.05). Serum enzymes such as AST, ALT, ACP, ALP, LDH and urea were altered significantly which in turn confirmed the damage of vital organ tissue.     

腹膜透析短期内对高血压患者多项指标的影响

目的研究腹膜透析短期内对高血压患者生化指标及免疫指标的影响。方法将48例2级高血压患者分为2组：腹膜透析组（CAPD组）和对照组。对照组不进行腹膜透析。连续6个月后观察。结果 CAPD组和高血压组的血清白蛋白、内皮素、血肾素以及血管紧张素Ⅱ治疗前无统计学差异,治疗后有统计学差异。两组的血红蛋白、血清K＋、血清Na＋于治疗前后并无差异。结论对于有腹膜透析需要的高血压患者,可以在短期内治疗基础病的同时降低高血压相关的生理、生化以及免疫指标。     

胆道手术后腹腔引流液中炎性因子检测对胆漏的早期诊断

目的探讨分析胆道术后腹腔引流液中炎性因子检测对胆漏的早期诊断价值。方法将160例胆道术后24h、48h、72h的腹腔引流液分别进行CRP、TNF-α、IL-6的检测,同时对不同原因引起的胆漏患者,进行腹腔引流液检测,对结果进行分析总结。结果 160例胆道手术的患者,术后出现胆漏13例,占8.13%,未出现胆漏147例,占91.87%。13例不同胆漏患者进行术后3次腹腔引流液中炎性因子检测平均结果：CRP（33.76±1.28）mg/L、TNF-α（45.83±2.37）ng/L、IL-6（33.68±4.51）ng/L。胆道患者手术后腹腔引流液中炎性因子均会引起不同程度的升高,而胆漏组患者的腹腔引流液中炎性因子明显高于未出现胆漏的患者。结论胆道术后腹腔引流液炎性因子检测CRP、TNF-α、IL-6早期异常升高,可以作为对早期胆漏的诊断参考依据。     

胃癌腹腔冲洗液多巴脱羧酶mRNA的检测

目的: 通过检测胃癌腹腔冲洗液中DDC mRNA的表达,探讨多巴胶羟酶(DDC)与胃癌腹膜转移的关系,并为胃癌腹膜转移预测和亚临床转移的筛检提供新的方法。 方法: 用RNA提取和RT-PCR方法检测92例胃癌腹腔冲洗液中DDC mRNA的表达,同时做腹腔冲洗细胞学(PLC)检查;10例良性疾病的腹腔冲洗液作为阴性对照。 结果: 92例胃癌腹腔冲洗液中有57例检测到DDC mRNA的表达(62.0%),其表达阳性率与胃癌腹膜转移的PS因素密切相关(P<0.05);且胃癌腹腔冲洗液中DDC mRNA的表达水平还与胃癌浸润深度、浆膜类型、PLC检查结果以及是否存在肉眼腹膜转移有关,浸润深度深、浆膜受侵程度重、PLC检查结果阳性及存在肉眼腹膜转移病灶者的DDC mRNA表达相对值明显升高(P<0.05);而10例良性疾病腹腔冲洗液均未检测到DDC mRNA的表达。 结论: 胃癌腹腔液中DDC mRNA的表达与胃癌腹膜转移密切相关,通过RT-PCR来检测DDC mRNA适用于胃癌腹膜转移的预测和亚临床转移的筛检。