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51.
Cecilia Nakid-Cordero Sylvain Choquet Nicolas Gauthier Noureddine Balegroune Nadine Tarantino Véronique Morel Nadia Arzouk Sonia Burrel Géraldine Rousseau Frédéric Charlotte Martin Larsen Vincent Vieillard Brigitte Autran Véronique Leblond Amélie Guihot for the K-VIROGREF Study Group 《American journal of transplantation》2021,21(8):2846-2863
EBV-positive and EBV-negative posttransplant lymphoproliferative disorders (PTLDs) arise in different immunovirological contexts and might have distinct pathophysiologies. To examine this hypothesis, we conducted a multicentric prospective study with 56 EBV-positive and 39 EBV-negative PTLD patients of the K-VIROGREF cohort, recruited at PTLD diagnosis and before treatment (2013–2019), and compared them to PTLD-free Transplant Controls (TC, n = 21). We measured absolute lymphocyte counts (n = 108), analyzed NK- and T cell phenotypes (n = 49 and 94), and performed EBV-specific functional assays (n = 16 and 42) by multiparameter flow cytometry and ELISpot-IFNγ assays (n = 50). EBV-negative PTLD patients, NK cells overexpressed Tim-3; the 2-year progression-free survival (PFS) was poorer in patients with a CD4 lymphopenia (CD4+<300 cells/mm3, p < .001). EBV-positive PTLD patients presented a profound NK-cell lymphopenia (median = 60 cells/mm3) and a high proportion of NK cells expressing PD-1 (vs. TC, p = .029) and apoptosis markers (vs. TC, p < .001). EBV-specific T cells of EBV-positive PTLD patients circulated in low proportions, showed immune exhaustion (p = .013 vs. TC) and poorly recognized the N-terminal portion of EBNA-3A viral protein. Altogether, this broad comparison of EBV-positive and EBV-negative PTLDs highlight distinct patterns of immunopathological mechanisms between these two diseases and provide new clues for immunotherapeutic strategies and PTLD prognosis. 相似文献
52.
Aurore Prunevieille Mohamed H. Babiker-Mohamed Colleen Aslami Bruno Gonzalez-Nolasco Nuala Mooney Gilles Benichou 《American journal of transplantation》2021,21(7):2583-2589
Extracellular vesicles, including exosomes, are regularly released by allogeneic cells after transplantation. Recipient antigen-presenting cells (APCs) capture these vesicles and subsequently display donor MHC molecules on their surface. Recent evidence suggests that activation of alloreactive T cells by the so-called cross-dressed APCs plays an important role in initiating the alloresponse associated with allograft rejection. On the other hand, whether allogeneic exosomes can bind to T cells on their own and activate them remains unclear. In this study, we showed that allogeneic exosomes can bind to T cells but do not stimulate them in vitro unless they are cultured with APCs. On the other hand, allogeneic exosomes activate T cells in vivo and sensitize mice to alloantigens but only when delivered in an inflammatory environment. 相似文献
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Jiang Bian Ting Wang Jijun Sun Xiaozhen He Zhijiao Wu Songmei Zhang Hao Chi Tingting Fan Shaowen Wang Weiyun Shi Qingguo Ruan 《American journal of transplantation》2021,21(12):3858-3870
The relevance of Tregs in the induction of tolerance against corneal allografts has been well established. Although it is well known that the conversion of Tregs into effector-like cells contributes to the loss of corneal immune privilege, the underlying mechanism is still not fully understood. Using heterologous penetrating keratoplasty model, we found that Tregs from corneal allograft rejected mice (inflam-Tregs) exhibit impaired function and characteristics of effector T cells. Further study showed that the expression of NF-κB c-Rel, a key mediator of effector T cell function, was significantly increased in inflam-Tregs. Mechanistic study revealed that elevated NF-κB c-Rel level in inflam-Tregs impaired Treg function through the promotion of inflammatory cytokine production and glycolysis. More importantly, we demonstrated that targeting NF-κB c-Rel was able to improve the immune suppressive function of inflam-Tregs in vitro and enhance the potential of them to suppress corneal transplantation rejection. Therefore, our current study identified NF-κB c-Rel as a key mediator of the conversion of Tregs into effector-like cells when under inflammatory environment. 相似文献
55.
我们的实验发现,磁处理水对环磷酰胺等五种抗癌药物的毒性具有不同程度的缓解作用,能增加小鼠的存活率,能降低药物引起的白细胞减少。对目前癌化疗中使用率最高的环磷酰胺,其作用最明显。 相似文献
56.
XU Xiu lin ZHU Nai shuo Department of medical instrumentation Shanghai medical instrumentation college China Lab.of molecular immunology School of life sciences Fudan University Shanghai China 《中国生物医学工程学报(英文版)》2002,(2)
INTRODUCTION From integrallevel and celllevel,the research of organism and diseasediagnosishave already entered molecular level.Molecular biology is the science to researchstructure and function of organism macromolecule.Based on differentgene code se-… 相似文献
57.
生态药理与生物药理—论中药药性理论的本质 总被引:1,自引:1,他引:0
目的:探讨中药药性理论的本质,方法:通过对医学人体理论,医学模式和药理理论内在关系的分析来剖析中药药性理论的本质,提出对中药药性理论本质的认识。结果:认为中药药性理论是传统中药药理理论,是适应中医学“生态-生物人体理论”的产物,中药药性理论是“生态药理”与“生物药理”相结合的产物即“生态-生物理”这是其本质所在,结论:传统中药药理即中药药性理论是“生态-生物药理”理论。 相似文献
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目的 :了解两型HLA B2 7分子在人细胞系内的合成、组装及转运过程。方法 :克隆到真核表达载体RSV 5neo的膜表面型与可溶型B2 7(sB2 7)cDNA以电转法转入人B淋巴母细胞突变细胞系C1R ,并使其稳定表达。以免疫沉淀、脉冲追踪及内切糖苷酶H消化实验观察两型B2 7分子在C1R细胞系内的细胞生物学特点。结果 :sB2 7分子与膜表面型B2 7分子一样 ,约需 30min完成细胞内的修饰过程 ,均与Calnexin及BiP这两种分子伴侣相关等 ,但与膜表面型B2 7分子相比 ,仅部分sB2 7分子被转运到细胞表面 ,相当一部分sB2 7分子 4h后仍滞留在内质网腔内。结论 :sB2 7分子在人C1R细胞内的合成、组装与转运过程与膜表面型B2 7分子相似 ,滞留在内质网腔的sB2 7分子的酶解片段有可能通过Ⅱ类分子而递呈给CD4 T细胞 ,这一现象将为研究HLA Ⅱ类分子与脊柱关节病的相关提供新线索 ,并为今后探讨B2 7及环境因素与脊柱关节病的相关、寻找早期诊断与早期治疗措施提供理论依据 相似文献
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