CK19、CyclinD1、HBME-1、34βE12、TPO、galectin-3在甲状腺乳头状癌组织中的表达及意义

目的探讨CK19、CyclinD1、HBME-1、34βE12、TPO、galectin-3在甲状腺乳头状癌（PTC）组织中的表达情况及其临床意义。方法应用免疫组化EnVision法,检测86例PTC、60例甲状腺滤泡性腺瘤（FTA）中CK19、CyclinD1、HBME-1、34βE12、TPO、galectin-3的表达情况。结果CK19和galectin-3表达PTC组织与滤泡性腺瘤比较无明显差异;PTC中CyclinD1、HBME-1、34βE12阳性表达率分别为93．02％、93．02％、90．69％,明显高于滤泡性腺瘤。PTC中TPO表达阳性率为2．33％,滤泡性腺瘤中其表达率为90．00％,两者比较有明显差异（P〈0．01）。结论HBME-1、CyclinD1、34βE12和TPO的联合检测,有助于提高PTC的确诊率,而CK19、galectin-3对鉴别PTC和FTA意义不明确。     

糖尿病肾病肾脏微小血管病变实验研究

目的:观察微血管损伤在糖尿病肾病中发病的关键作用。方法:以单侧肾切除并链脲佐菌素尾静脉注射建立糖尿病肾病模型,测定动态观察4、8、12、16、20周肾小管周围毛细血管网(PTC)、血管内膜厚度、肾小球硬化指数、肾小球毛细血管袢、肾小球体积、细胞外基质等指标。结果:随造模时间延长,糖尿病肾病组大鼠肾小球毛细血管袢开放面积减少,肾小球体积增大,肾间质PTC网减少,动脉内膜增厚,肾小球硬化指数、系膜基质增加。与假手术组比较均有统计学意义(P<0.05或P<0.01)。结论:肾间质PTC网与肾小球硬化呈明显负相关,PTC网越少,肾小球毛细血管褂开放面积减少,肾小球硬化更严重;同时PTC网减少导致肾缺血也是肾间质纤维化和肾乳头坏死的原因。     

LRRC52-AS1 is associated with clinical progression and regulates cell migration and invasion in papillary thyroid cancer

The incidence of thyroid cancer has increased in recent decades. The potential molecular mechanisms of papillary thyroid cancer (PTC) are still to be uncovered. In recent years, a number of studies reported that LRRC super family members are up-regulated in cancer cells. Cancer cells can experience a feature change from an epithelial to a mesenchymal phenotype, which is called epithelial–mesenchymal transition (EMT) during cancer progression. We found that LRRC52-AS1 is highly expressed in PTC cell lines rather than normal tissues and this observation was consistent with The Cancer Genome Atlas (TCGA) cohort. In a word, LRRC52-AS1 is associated with clinical progression in papillary thyroid cancer. In vitro experiments showed that knocking down LRRC52-AS1 significantly decreased the migration and invasion of the PTC cell lines (BCPAP and TPC). Meanwhile, LRRC52-AS1 may influence the progress of papillary thyroid cancer via mesenchymal markers N-cadherin, vimentin and TAZ. The LRRC52-AS1 gene is up-regulated in papillary thyroid cancer, and knockdown of LRRC52-AS1 could restrain cellular migration, and invasion in vitro. This study indicated that LRRC52-AS1 is a gene associated with PTC and might become a potential therapeutic target in PTC.     

Hepatobiliary tuberculosis

Tuberculosis is known to involve the liver in different ways. The term hepatobiliary tuberculosis refers to the localized form of hepatic tuberculosis as a distinct clinical entity, with signs and symptoms related to the hepatobiliary tract. Its clinical features and the different diagnostic aids used in its diagnosis are reviewed. Plain abdominal radiographs showing diffuse hepatic calcifications seen in approximately 50% of cases are almost diagnostic for hepatobiliary tuberculosis. Liver biopsies obtained either by ultrasound, computed tomography or laparoscopy, showing caseating granuloma usually establish the diagnosis. In the absence of caseation necrosis, a positive acid-fast bacillus (AFB) or culture for Mycobacterium tuberculosis is needed to establish the diagnosis. A polymerase chain reaction assay for the identification of Mycobacterium tuberculosis in liver biopsy specimens is a new development. Treatment is similar to that used for pulmonary tuberculosis. Quadruple therapy (using four anti-tuberculosis drugs) is recommended, generally for 1 year. For patients with obstructive jaundice, in addition to anti-tuberculous treatment, biliary decompression should be performed either by stent insertion during endoscopic retrograde cholangiopancreatology, by percutaneous transhepatic biliary drainage or by surgical decompression whenever feasible.     

1000例黑龙江地区汉族青年苯硫脲尝味能力的测定

本文通过黑龙江地区正常男,女学生各500人的PTC尝味能力的调查,统计出PTC尝味能力的表现型频率,并根据Hardy-weinberg定律计算出PTC尝味基因频率,隐性基因频率为0.378,显性基因频率为0.622。男女味盲比率分别为14.4％和12.4％差异不显著(P<0.05),总的味盲频率为13.4％,要比其它地区汉族人稍高,这可能是有少数民族的基因融合所致。     

相转移催化N-烷基化反应在药物合成中的应用

综述了相转移催化N-烷基化反应在药物合成上的应用.     

Histological analysis of late renal allografts of antidonor antibody positive patients with C4d deposits in peritubular capillaries

Abstract:  The association of humoral immunity with late renal allograft dysfunction has recently been recognized, and many reports have revealed C4d deposits in peritubular capillaries (C4d in PTC), and the presence of serum antidonor HLA antibody in patients suffering from graft dysfunction, long time after transplantation. In this study, morphological changes in renal allograft biopsies more than 1 year after transplantation in 14 patients with C4d in PTC and serum antidonor antibody were investigated for the presence of chronic rejection (CR). In addition to the light microscope study, an electron microscope study was done to evaluate the multilayering of the peritubular capillary basement membrane (MLPTC). Histologically, only seven of 14 patients met the criteria of CR, and 71.4% (5/7) of CR patients had episodes of acute humoral rejection (AHR), coexisting with acute tubulointerstitial rejection. Peritubular capillaritis was observed in all patients, although it differed in severity. Transplant glomerulitis and interstitial inflammation were also observed in many patients: 71.4% (10/14) and 92.9% (13/14) respectively. MLPTC was observed in 12 patients (85.7%), but the severity of the MLPTC did not reflect the severity of peritubular capillaritis or any other histological features. The long-term outcomes of the patients CR, especially those with episodes of AHR, were poor, and two of them lost their graft functions. On the other hand, patients without CR had relatively favourable outcomes. In conclusion, we confirmed the diverse morphological changes of late renal allografts, which cannot be categorized as chronic humoral rejection (CHR), and such patients who do not have typical morphological changes such as CHR, should be followed-up on a long-term basis in order to clarify the significance of C4d on PTC in late renal allografts.     

Postoperative imaging of living donor liver transplantation complications

Background

There is no doubt that the role of Different diagnostic imaging is well established in the evaluation of patients who are being evaluated for potential liver transplantation but it plays a huge role in the success of transplanted liver operations. Technical advances in imaging equipment and techniques allow more accurate assessment of postoperative living donor transplantation complications.

乳头状甲状腺癌中脂质代谢相关基因表达的筛选和验证

目的:探讨乳头状甲状腺癌（papillary thyroid carcinoma,PTC）中脂质代谢相关基因的变化。方法:应用3组新鲜的PTC癌组织及癌旁甲状腺组织,采用RT2 ProfilerTM PCR ARRAY(Qiagen)进行脂质代谢基因的筛选,探讨PTC癌及癌旁甲状腺组织中表达差异较大的基因。采用RT-PCR法在46对PTC癌及癌旁甲状腺组织中对筛选得到的基因表达差异较大的基因进行验证。结果:经过筛选及验证发现,SLC27A6(FATP6)、FABP3在PTC癌组织中的表达高于癌旁甲状腺组织（P＜0.05）。结论:PTC癌组织中FATP6及FABP3在mRNA水平上的表达高于癌旁甲状腺组织,上述分子及其参与的脂肪酸代谢可能成为PTC诊治的新靶点。